{"id":"https://openalex.org/W2894741101","doi":null,"title":"Identification and Characterization of Effector Proteins and Genomic Analysis of the Cucurbit Powdery Mildew Golovinomyces cichoracearum","display_name":"Identification and Characterization of Effector Proteins and Genomic Analysis of the Cucurbit Powdery Mildew Golovinomyces cichoracearum","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2894741101","mag":"2894741101"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt7903s3kd","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/7903s3kd","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5057375792","display_name":"Kate Elizabeth Scheibel","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Kate Elizabeth Scheibel","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5057375792"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13214","display_name":"Powdery Mildew Fungal Diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T13214","display_name":"Powdery Mildew Fungal Diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10076","display_name":"Plant-Microbe Interactions and Immunity","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10733","display_name":"Wheat and Barley Genetics and Pathology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/golovinomyces-cichoracearum","display_name":"Golovinomyces cichoracearum","score":0.9990000128746033},{"id":"https://openalex.org/keywords/powdery-mildew","display_name":"powdery mildew","score":0.902999997138977},{"id":"https://openalex.org/keywords/effectors","display_name":"effectors","score":0.6639999747276306},{"id":"https://openalex.org/keywords/haustorium-formation","display_name":"haustorium formation","score":0.8040000200271606},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.8119999766349792},{"id":"https://openalex.org/keywords/plant-immune-suppression","display_name":"plant immune suppression","score":0.7990000247955322},{"id":"https://openalex.org/keywords/hypersensitive-response","display_name":"hypersensitive response","score":0.8209999799728394},{"id":"https://openalex.org/keywords/fungal-virulence","display_name":"fungal virulence","score":0.628000020980835},{"id":"https://openalex.org/keywords/fungal-genome","display_name":"fungal genome","score":0.3109999895095825}],"concepts":[{"id":"https://openalex.org/C2779336322","wikidata":"https://www.wikidata.org/wiki/Q934008","display_name":"Powdery mildew","level":2,"score":0.9862096905708313},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.789201021194458},{"id":"https://openalex.org/C2779669290","wikidata":"https://www.wikidata.org/wiki/Q1220207","display_name":"Mildew","level":2,"score":0.642560601234436},{"id":"https://openalex.org/C2779678110","wikidata":"https://www.wikidata.org/wiki/Q764","display_name":"Fungus","level":2,"score":0.5904746055603027},{"id":"https://openalex.org/C2779491563","wikidata":"https://www.wikidata.org/wiki/Q157892","display_name":"Arabidopsis","level":4,"score":0.5868669152259827},{"id":"https://openalex.org/C93678976","wikidata":"https://www.wikidata.org/wiki/Q4215946","display_name":"Plant disease resistance","level":3,"score":0.5282924175262451},{"id":"https://openalex.org/C126831891","wikidata":"https://www.wikidata.org/wiki/Q221673","display_name":"Host (biology)","level":2,"score":0.44348084926605225},{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.43682241439819336},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.407754123210907},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.40411263704299927},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3401505649089813},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt7903s3kd","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/7903s3kd","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:2894741101","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/7903s3kd","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Zero hunger","score":0.9208999872207642,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7057495848","https://openalex.org/W1894078792","https://openalex.org/W7084124339","https://openalex.org/W2150010045","https://openalex.org/W3092052076","https://openalex.org/W4254529370","https://openalex.org/W4406292181","https://openalex.org/W2913936290","https://openalex.org/W2806974693","https://openalex.org/W2100516809"],"abstract_inverted_index":{"Powdery":[0],"mildew,":[1,42],"a":[2,27,85,91,97,106,359,442,487,572,661,701],"fungal":[3,237,247],"disease":[4,16,52,431,713],"of":[5,9,14,24,30,49,59,74,77,81,160,171,179,191,194,200,213,228,280,287,299,401,486,503,515,533,579,599,611,617,644,665,672,682,705],"plants,":[6],"is":[7,45,130,154,305,312,437,441,448,456],"one":[8],"the":[10,46,64,72,75,101,133,140,149,158,161,166,172,177,189,198,225,285,297,300,315,333,345,351,366,372,402,409,424,434,459,513,519,525,531,557,561,577,597,600,609,615,642,645,673,677,683,706],"most":[11],"significant":[12],"causes":[13,712],"crop":[15],"and":[17,37,118,126,135,147,168,176,181,232,245,262,293,311,318,327,375,405,447,462,469,553,596,636,651,679],"yield":[18],"loss":[19],"worldwide.":[20],"Collectively,":[21],"this":[22,60,78,192,218,252,605,710],"group":[23],"pathogens":[25,83],"infects":[26],"diverse":[28],"set":[29],"plant":[31,66,82,102,113,150,288,301,316,334,352,367,389,410,425,460,685],"hosts,":[32],"including":[33],"wheat,":[34],"barley,":[35],"grape,":[36],"ornamental":[38],"species.":[39,56,548],"One":[40],"such":[41],"Golovinomyces":[43,637],"cichoracearum,":[44,505],"causative":[47],"agent":[48],"powdery":[50,173,229,378,403,444,493,546,621],"mildew":[51,61,174,230,379,445,494,547,622],"on":[53,269,714],"susceptible":[54,92],"cucurbit":[55],"The":[57,108],"ability":[58,514],"to":[62,90,121,142,157,163,254,274,337,371,377,429,473,512,517,575,614],"infect":[63],"model":[65,361,488],"Arabidopsis":[67,324,466],"thaliana":[68],"has":[69,539],"allowed":[70],"for":[71,123,188,205,267,489,530,589],"study":[73,190],"biology":[76,681],"important":[79],"class":[80,193],"in":[84,112,241,278,296,314,332,362,458,544,560,649],"laboratory":[86],"setting.":[87],"When":[88],"exposed":[89],"host,":[93],"G.":[94,208,257,504,586,612,687],"cichoracearum":[95,209,258,587,613],"forms":[96],"feeding":[98],"structure":[99],"within":[100],"cell,":[103],"known":[104,131],"as":[105,307,451],"haustorium.":[107],"fungus":[109,141,162,711],"initiates":[110],"changes":[111,146],"cell":[114],"structure,":[115],"gene":[116,281,652],"expression,":[117,282],"nutrient":[119],"transport":[120],"allow":[122,529],"its":[124,715],"survival":[125],"reproduction.":[127],"Relatively":[128],"little":[129],"about":[132],"molecular":[134,581,692],"cellular":[136,145],"mechanisms":[137,707],"employed":[138,223],"by":[139,350,708],"elicit":[143],"these":[144,276],"evade":[148],"immune":[151,302,335,368],"response.":[152,303],"This":[153,527],"partially":[155],"due":[156],"recalcitrance":[159],"genetic":[164,180,536],"manipulation,":[165],"large":[167],"complicated":[169],"nature":[170],"genome,":[175],"lack":[178],"genomic":[182,583],"tools":[183],"that":[184,217,233,264,341,419,563,641,657],"have":[185,482,700],"been":[186,542],"developed":[187,574],"organisms.Here,":[195],"we":[196,481,507,555,570,655,698],"describe":[197,272,497,571],"development":[199],"an":[201,235,308,413,452],"Agrobacterium":[202],"tumefaciens-mediated":[203],"tool":[204],"transiently":[206],"silencing":[207,234],"genes":[210],"during":[211,492],"infection":[212],"Arabidopsis.":[214],"We":[215,249,339,357,417,607,639],"demonstrate":[216],"technique":[219,253],"can":[220],"be":[221],"successfully":[222],"at":[224],"early":[226],"stages":[227],"infection,":[231],"essential":[236],"gene,":[238],"GcCYP51,":[239],"results":[240],"reduced":[242],"haustorial":[243],"formation":[244],"subsequent":[246],"growth.":[248],"then":[250,271],"use":[251],"identify":[255],"three":[256],"effectors,":[259],"GcEC8,":[260],"GcEC10,":[261],"GcEC17,":[263],"are":[265,647],"required":[266],"virulence":[268],"Arabidopsis.We":[270],"efforts":[273,498,550],"characterize":[275],"effectors":[277],"terms":[279],"sub-cellular":[283],"localization,":[284],"identification":[286],"interacting":[289],"partners,":[290],"bioinformatic":[291],"prediction,":[292],"their":[294],"roles":[295],"suppression":[298],"GcEC10":[304,320,342,364],"characterized":[306,450],"RNase-like":[309,453],"protein":[310,384,411],"localized":[313,457],"cytosol":[317],"nucleus.":[319],"may":[321,406,421,566],"interact":[322,407,474],"with":[323,382,388,397,408,423,475,694],"proteins":[325,330,399],"AtEDR4":[326],"AtPHOS32,":[328],"two":[329],"implicated":[331],"response":[336,347,369,374],"pathogens.":[338],"determined":[340],"expression":[343],"suppresses":[344],"hypersensitive":[346,373],"(HR)":[348],"elicited":[349],"resistance":[353],"gene/effector":[354],"pair":[355],"Bs2/AvrBs2.":[356],"propose":[358,418],"potential":[360],"which":[363,506,538,709],"attenuates":[365],"leading":[370,428],"immunity":[376],"via":[380,477],"interference":[381],"mitogen-activated":[383],"kinase":[385],"cascades":[386],"associated":[387],"immunity.GcEC8":[390],"shares":[391],"no":[392],"sequence":[393,602],"or":[394],"domain":[395],"homology":[396],"any":[398,545],"outside":[400],"mildews,":[404],"AXR3,":[412],"auxin-responsive":[414],"transcription":[415],"factor.":[416],"GcEC8":[420],"interfere":[422],"auxin":[426],"response,":[427],"increased":[430],"resistance,":[432],"although":[433],"specific":[435],"mechanism":[436],"not":[438,483,540],"yet":[439,484,541],"known.GcEC17":[440],"highly-conserved":[443],"effector,":[446],"also":[449],"protein.":[454],"GcEc17":[455],"nucleus":[461],"cytosol.":[463],"Two":[464],"hypothetical":[465],"genes,":[467],"At4g29905":[468],"At3g32930,":[470],"were":[471,551],"predicted":[472,666],"GcEC17":[476,490],"yeast":[478],"two-hybrid,":[479],"however":[480,654],"conceived":[485],"action":[491],"infection.We":[495],"further":[496],"towards":[499],"transient":[500],"plasmid":[501],"transformation":[502],"hope":[508],"will":[509],"eventually":[510],"lead":[511],"researchers":[516,565],"introduce":[518],"Cas9":[520],"genome":[521,601,610,678],"editing":[522],"system":[523],"into":[524,676],"fungus.":[526],"might":[528],"creation":[532],"stable,":[534],"targeted":[535],"mutants,":[537],"achieved":[543],"Our":[549],"unsuccessful,":[552],"here":[554],"detail":[556],"attempted":[558],"methodologies":[559],"hopes":[562],"future":[564],"find":[567],"more":[568,702],"success.Finally,":[569],"method":[573],"achieve":[576],"purification":[578],"high":[580],"weight":[582],"DNA":[584],"from":[585],"suitable":[588],"Pacific":[590],"Biosciences":[591],"Single":[592],"Molecule":[593],"Long-Read":[594],"Sequencing,":[595],"analysis":[598],"obtained":[603],"using":[604],"method.":[606],"compare":[608],"genomes":[616,643],"four":[618],"other":[619],"sequenced":[620],"species,":[623],"Blumeria":[624],"graminis":[625,630],"f.":[626,631],"sp.":[627,632],"hordei,":[628,633],"B.":[629],"Erysiphe":[634],"pisi,":[635],"orontii.":[638],"found":[640,656],"mildews":[646],"similar":[648],"size":[650],"content,":[653],"each":[658],"species":[659],"encodes":[660],"large,":[662],"unique":[663],"repertoire":[664],"effector":[667,680],"proteins.These":[668],"experiments":[669],"provide":[670],"some":[671],"first":[674],"insights":[675],"enigmatic":[684],"pathogen":[686],"cichoracearum.":[688],"By":[689],"combining":[690],"novel":[691],"techniques":[693],"next-generation":[695],"sequencing":[696],"approaches,":[697],"now":[699],"complete":[703],"idea":[704],"host":[716],"plants.":[717]},"counts_by_year":[],"updated_date":"2026-09-09T07:26:56.047234","created_date":"2018-10-12T00:00:00"}