{"id":"https://openalex.org/W2891237199","doi":"https://doi.org/10.1111/jfbc.12686","title":"Purification and characterization of alpha\u2010amylase inhibitor from the seeds of underutilized legume,Mucuna pruriens","display_name":"Purification and characterization of alpha\u2010amylase inhibitor from the seeds of underutilized legume,Mucuna pruriens","publication_year":2018,"publication_date":"2018-09-19","ids":{"openalex":"https://openalex.org/W2891237199","doi":"https://doi.org/10.1111/jfbc.12686","mag":"2891237199"},"language":"en","primary_location":{"id":"doi:10.1111/jfbc.12686","doi":"https://doi.org/10.1111/jfbc.12686","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jfbc.12686","pdf_url":null,"source":{"id":"https://openalex.org/S53703007","display_name":"Journal of Food Biochemistry","issn_l":"0145-8884","issn":["0145-8884","1745-4514"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Food Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5083230587","display_name":"Rajiv P. Bharadwaj","orcid":"https://orcid.org/0000-0001-5095-1726","observed_orcids":["https://orcid.org/0000-0001-5095-1726"]},"institutions":[{"id":"https://openalex.org/I19008122","display_name":"Mangalore University","ror":"https://ror.org/05fep3933","country_code":"IN","type":"education","lineage":["https://openalex.org/I19008122"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rajiv Pranesh Bharadwaj","raw_affiliation_strings":["Department of Biochemistry, Mangalore University PG Centre, Chikka Aluvara, India"],"raw_orcid":"https://orcid.org/0000-0001-5095-1726","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Mangalore University PG Centre, Chikka Aluvara, India","institution_ids":["https://openalex.org/I19008122"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004228566","display_name":"Narayanappa Govinda Raju","orcid":"https://orcid.org/0000-0003-3613-0889","observed_orcids":["https://orcid.org/0000-0003-3613-0889"]},"institutions":[{"id":"https://openalex.org/I342246540","display_name":"Karnataka State Open University","ror":"https://ror.org/00ecztp88","country_code":"IN","type":"education","lineage":["https://openalex.org/I342246540"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Narayanappa Govinda Raju","raw_affiliation_strings":["Department of Biotechnology, Karnataka State Open University, Mysuru, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Karnataka State Open University, Mysuru, India","institution_ids":["https://openalex.org/I342246540"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030352205","display_name":"Kempohalli Sayanna Chandrashekharaiah","orcid":"https://orcid.org/0000-0002-5855-6779","observed_orcids":["https://orcid.org/0000-0002-5855-6779"]},"institutions":[{"id":"https://openalex.org/I19008122","display_name":"Mangalore University","ror":"https://ror.org/05fep3933","country_code":"IN","type":"education","lineage":["https://openalex.org/I19008122"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Kempohalli Sayanna Chandrashekharaiah","raw_affiliation_strings":["Department of Biochemistry, Mangalore University PG Centre, Chikka Aluvara, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Mangalore University PG Centre, Chikka Aluvara, India","institution_ids":["https://openalex.org/I19008122"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5030352205"],"corresponding_institution_ids":["https://openalex.org/I19008122"],"apc_list":{"value":2700,"currency":"USD","value_usd":2700},"apc_paid":null,"fwci":1.0908,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.7633539,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"42","issue":"6","first_page":"e12686","last_page":"e12686"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11140","display_name":"Natural Antidiabetic Agents Studies","score":0.9535999894142151,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11140","display_name":"Natural Antidiabetic Agents Studies","score":0.9535999894142151,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.017500000074505806,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12257","display_name":"Bioactive natural compounds","score":0.011099999770522118,"subfield":{"id":"https://openalex.org/subfields/3002","display_name":"Drug Discovery"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha-amylase-inhibitors","display_name":"alpha-amylase inhibitors","score":0.9760000109672546},{"id":"https://openalex.org/keywords/mucuna-pruriens","display_name":"Mucuna pruriens","score":0.996999979019165},{"id":"https://openalex.org/keywords/human-salivary-alpha-amylase","display_name":"human salivary alpha-amylase","score":0.871999979019165},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.5950000286102295},{"id":"https://openalex.org/keywords/ph-stability","display_name":"pH stability","score":0.6549999713897705},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.6010000109672546},{"id":"https://openalex.org/keywords/antidiabetic-drugs","display_name":"antidiabetic drugs","score":0.6539999842643738},{"id":"https://openalex.org/keywords/obesity","display_name":"obesity","score":0.5789999961853027},{"id":"https://openalex.org/keywords/underutilized-legumes","display_name":"underutilized legumes","score":0.8050000071525574},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.5669999718666077}],"concepts":[{"id":"https://openalex.org/C170835558","wikidata":"https://www.wikidata.org/wiki/Q17153","display_name":"Amylase","level":3,"score":0.7645381689071655},{"id":"https://openalex.org/C163994747","wikidata":"https://www.wikidata.org/wiki/Q2916358","display_name":"Sephadex","level":3,"score":0.6965333223342896},{"id":"https://openalex.org/C2776201759","wikidata":"https://www.wikidata.org/wiki/Q953611","display_name":"Mucuna pruriens","level":2,"score":0.6684020757675171},{"id":"https://openalex.org/C173959671","wikidata":"https://www.wikidata.org/wiki/Q106951023","display_name":"Alpha-amylase","level":4,"score":0.6354031562805176},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.514917254447937},{"id":"https://openalex.org/C2776632002","wikidata":"https://www.wikidata.org/wiki/Q145909","display_name":"Legume","level":2,"score":0.5084549188613892},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.502112627029419},{"id":"https://openalex.org/C97428945","wikidata":"https://www.wikidata.org/wiki/Q15305109","display_name":"Fractionation","level":2,"score":0.4396134316921234},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.41384172439575195},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.313160240650177},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.27739977836608887},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.1613900363445282}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1111/jfbc.12686","doi":"https://doi.org/10.1111/jfbc.12686","is_oa":false,"landing_page_url":"https://doi.org/10.1111/jfbc.12686","pdf_url":null,"source":{"id":"https://openalex.org/S53703007","display_name":"Journal of Food Biochemistry","issn_l":"0145-8884","issn":["0145-8884","1745-4514"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Food Biochemistry","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.5683000087738037,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W194912786","https://openalex.org/W1494154021","https://openalex.org/W1497665647","https://openalex.org/W1501568305","https://openalex.org/W1517995104","https://openalex.org/W1775749144","https://openalex.org/W1906057256","https://openalex.org/W1982544261","https://openalex.org/W1987718964","https://openalex.org/W1991484014","https://openalex.org/W1995737779","https://openalex.org/W1995774023","https://openalex.org/W2003328401","https://openalex.org/W2012898950","https://openalex.org/W2018048818","https://openalex.org/W2018564776","https://openalex.org/W2019521836","https://openalex.org/W2030707876","https://openalex.org/W2032186668","https://openalex.org/W2032461580","https://openalex.org/W2040298027","https://openalex.org/W2043211061","https://openalex.org/W2050335943","https://openalex.org/W2058271302","https://openalex.org/W2058397950","https://openalex.org/W2059684897","https://openalex.org/W2079637435","https://openalex.org/W2113180930","https://openalex.org/W2115707978","https://openalex.org/W2116393128","https://openalex.org/W2132876055","https://openalex.org/W2138942193","https://openalex.org/W2142369423","https://openalex.org/W2180208960","https://openalex.org/W2187182683","https://openalex.org/W2208451345","https://openalex.org/W2765412986","https://openalex.org/W2792825461"],"related_works":["https://openalex.org/W2133034244","https://openalex.org/W2023157388","https://openalex.org/W7064446612","https://openalex.org/W1972329744","https://openalex.org/W1994340387","https://openalex.org/W4404058639","https://openalex.org/W2375525667","https://openalex.org/W1968956861","https://openalex.org/W2056904149","https://openalex.org/W2363698168"],"abstract_inverted_index":{"An":[0],"\u03b1-amylase":[1,135,179],"inhibitor":[2,61,65,82,121,136,161],"(EC":[3],"3.2.1.1)":[4],"was":[5,46,77,83,95,108,122,162],"purified":[6,57,80,117],"by":[7,29,212],"buffer":[8],"extraction,":[9],"ammonium":[10],"sulfate":[11],"fractionation,":[12],"CM-cellulose,":[13],"and":[14,36,42,52,73,88,148,196,207,217],"sephadex":[15],"G-75":[16],"chromatography":[17,32],"from":[18,178],"the":[19,40,74,113,126,166,183,192],"soaked":[20],"seeds":[21],"of":[22,44,67,71,101,106,116,128,134,146,150,168,174,194,228],"Mucuna":[23,58],"pruriens.":[24],"The":[25,56,79,172],"molecular":[26],"weight":[27],"determined":[28],"gel":[30],"permeation":[31],"on":[33,112],"Sephadex":[34],"G-100":[35],"SDS-PAGE,":[37],"both":[38],"in":[39,144,155,182,191,204,215,234,239],"presence":[41],"absence":[43],"2-mercaptoethanol,":[45],"found":[47,84,96,123,163,203],"to":[48,85,97,124,164],"be":[49,86,220],"27.24":[50],"kDa":[51],"25.6":[53],"kDa,":[54],"respectively.":[55],"pruriens":[59],"amylase":[60,81,120,160],"showed":[62],"a":[63,223],"specific":[64],"activity":[66,91,127,133,167,177],"61.18,":[68],"fold":[69],"purity":[70],"36.68,":[72],"yield":[75],"obtained":[76],"14.01%.":[78],"heat-stable":[87],"retained":[89],"80.50%":[90],"at":[92],"65\u00b0C.":[93],"Inhibitor":[94],"have":[98],"pH":[99],"optima":[100],"6.9.":[102],"Hundred":[103],"percent":[104],"zone":[105],"inhibition":[107],"observed":[109],"when":[110],"added":[111],"plated":[114],"organisms":[115],"inhibitor.":[118],"Purified":[119,159],"inhibit":[125,165],"human":[129,169,241],"salivary":[130,170],"\u03b1-amylase.":[131,171],"Inhibitory":[132],"against":[137],"mammalian":[138,184],"amylases":[139],"could":[140,186],"suggest":[141],"its":[142,237],"potential":[143,173,224],"treatment":[145],"diabetes":[147],"cure":[149],"nutritional":[151,195],"problems,":[152],"which":[153,233],"results":[154],"obesity.":[156],"Practical":[157],"applications":[158,238],"this":[175],"inhibitory":[176],"inhibitors,":[180,232],"especially":[181],"\u03b1-amylase,":[185],"play":[187],"an":[188,200],"important":[189],"role":[190],"management":[193],"diabetes-related":[197],"disorders.":[198],"Mucuna,":[199],"underutilized":[201],"legume":[202],"tropical":[205],"region":[206],"also":[208],"cultivated":[209],"as":[210,222],"food":[211],"various":[213,240],"tribal's":[214],"Asia":[216],"Africa":[218],"can":[219],"used":[221],"source":[225],"for":[226],"extraction":[227],"these":[229],"beneficiary":[230],"protease":[231],"turn":[235],"finds":[236],"therapeutic":[242],"and/or":[243],"disorder":[244],"management.":[245]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2018-09-27T00:00:00"}