{"id":"https://openalex.org/W2889405641","doi":"https://doi.org/10.1055/s-0038-1642924","title":"AMINO ACID SEQUENCE OF THE THROMBIN-BINDING SITE ON PLATELET GLYCOPROTEIN Ib","display_name":"AMINO ACID SEQUENCE OF THE THROMBIN-BINDING SITE ON PLATELET GLYCOPROTEIN Ib","publication_year":1987,"publication_date":"1987-01-01","ids":{"openalex":"https://openalex.org/W2889405641","doi":"https://doi.org/10.1055/s-0038-1642924","mag":"2889405641"},"language":"en","primary_location":{"id":"doi:10.1055/s-0038-1642924","doi":"https://doi.org/10.1055/s-0038-1642924","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1642924","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086388019","display_name":"Kazuo Yamamoto","orcid":"https://orcid.org/0000-0001-7466-2443","observed_orcids":["https://orcid.org/0000-0001-7466-2443"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Yamamoto","raw_affiliation_strings":["Depts. of Cardiovascular Res, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Depts. of Cardiovascular Res, Tokyo 113 Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066586146","display_name":"Hiroshi Kawasaki","orcid":"https://orcid.org/0000-0002-2517-159X","observed_orcids":["https://orcid.org/0000-0002-2517-159X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Kawasaki","raw_affiliation_strings":["Molecular Biol, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biol, Tokyo 113 Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050949810","display_name":"Kenji Suzuki","orcid":"https://orcid.org/0000-0002-3993-8309","observed_orcids":["https://orcid.org/0000-0002-3993-8309"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Suzuki","raw_affiliation_strings":["Molecular Biol, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Molecular Biol, Tokyo 113 Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005757550","display_name":"Kenjiro Tanoue","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"K Tanoue","raw_affiliation_strings":["Depts. of Cardiovascular Res, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Depts. of Cardiovascular Res, Tokyo 113 Japan","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109314228","display_name":"G K\u014dsaki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3020605285","display_name":"Tokyo Metropolitan Komagome Hospital","ror":"https://ror.org/04eqd2f30","country_code":"JP","type":"healthcare","lineage":["https://openalex.org/I3020605285"]},{"id":"https://openalex.org/I4210098918","display_name":"Tokyo Metropolitan Institute of Medical Science","ror":"https://ror.org/00vya8493","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4210098918"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"G Kosaki","raw_affiliation_strings":["Tokyo Metropolitan Inst, of Med. Sci., and Tokyo Metropolitan Komagome Hospital, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Metropolitan Inst, of Med. Sci., and Tokyo Metropolitan Komagome Hospital, Tokyo 113 Japan","institution_ids":["https://openalex.org/I3020605285","https://openalex.org/I4210098918"]}]},{"author_position":"last","author":{"id":null,"display_name":"H Yamazaki","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Yamazaki","raw_affiliation_strings":["Depts. of Cardiovascular Res, Tokyo 113 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Depts. of Cardiovascular Res, Tokyo 113 Japan","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.50748621,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.006300000008195639,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10831","display_name":"Cell Adhesion Molecules Research","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2723","display_name":"Immunology and Allergy"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/platelet-glycoprotein-ib","display_name":"platelet glycoprotein Ib","score":0.8519999980926514},{"id":"https://openalex.org/keywords/thrombin-binding-site","display_name":"thrombin binding site","score":0.972000002861023},{"id":"https://openalex.org/keywords/amino-acid-sequence","display_name":"amino acid sequence","score":0.8840000033378601},{"id":"https://openalex.org/keywords/thrombin-induced-platelet-aggregation","display_name":"thrombin-induced platelet aggregation","score":0.8970000147819519},{"id":"https://openalex.org/keywords/peptide-analogs","display_name":"peptide analogues","score":0.5889999866485596},{"id":"https://openalex.org/keywords/thrombin-receptor-antagonist","display_name":"thrombin receptor antagonist","score":0.5040000081062317},{"id":"https://openalex.org/keywords/platelet-membrane-glycoproteins","display_name":"platelet membrane glycoproteins","score":0.6179999709129333},{"id":"https://openalex.org/keywords/platelet-inhibition","display_name":"platelet aggregation inhibition","score":0.5009999871253967},{"id":"https://openalex.org/keywords/human-platelets","display_name":"human platelets","score":0.4390000104904175}],"concepts":[{"id":"https://openalex.org/C2777292125","wikidata":"https://www.wikidata.org/wiki/Q409166","display_name":"Thrombin","level":3,"score":0.7912936210632324},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6643308401107788},{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.5989512801170349},{"id":"https://openalex.org/C2779881004","wikidata":"https://www.wikidata.org/wiki/Q7451876","display_name":"Sepharose","level":3,"score":0.5863914489746094},{"id":"https://openalex.org/C2778401398","wikidata":"https://www.wikidata.org/wiki/Q383836","display_name":"Chymotrypsin","level":4,"score":0.5816546678543091},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.540225625038147},{"id":"https://openalex.org/C179247698","wikidata":"https://www.wikidata.org/wiki/Q382799","display_name":"Affinity chromatography","level":3,"score":0.4842071533203125},{"id":"https://openalex.org/C107824862","wikidata":"https://www.wikidata.org/wiki/Q616005","display_name":"Binding site","level":2,"score":0.4636795222759247},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.45523881912231445},{"id":"https://openalex.org/C2777824588","wikidata":"https://www.wikidata.org/wiki/Q414783","display_name":"Dithiothreitol","level":3,"score":0.44844067096710205},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.42395615577697754},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.37613314390182495},{"id":"https://openalex.org/C89560881","wikidata":"https://www.wikidata.org/wiki/Q101026","display_name":"Platelet","level":2,"score":0.2690293788909912},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.23487010598182678},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1519627869129181},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1055/s-0038-1642924","doi":"https://doi.org/10.1055/s-0038-1642924","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1642924","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/6","score":0.49000000953674316,"display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2410107450","https://openalex.org/W1548847180","https://openalex.org/W2438199954","https://openalex.org/W2587018063","https://openalex.org/W1969303122","https://openalex.org/W7097389772","https://openalex.org/W1974933326","https://openalex.org/W2018382750","https://openalex.org/W2109935864","https://openalex.org/W2463800070"],"abstract_inverted_index":{"Amino":[0],"acid":[1,235,243,250],"sequence":[2,236,244],"of":[3,17,89,127,217,267,279,283],"the":[4,15,18,71,100,132,142,162,190,201,208,214,277],"thrombin-binding":[5,209],"site":[6,210],"on":[7,22,213,220,247],"platelet":[8,28],"glycoprotein":[9],"(GP)":[10],"Ib":[11,94],"was":[12,39,68,74,104,119,145,179,229,237],"determined":[13],"and":[14,35,73,112,129,153,159,166,184,231],"effects":[16],"synthesized":[19],"analogous":[20,253],"peptides":[21,258],"thrombin-induced":[23,265],"aggregation":[24],"were":[25,47,114,155,168,255,275],"studied.":[26],"Crude":[27],"membrane":[29],"fraction":[30],"solubilized":[31],"with":[32,49,76,106,194,196],"5mM":[33],"dithiothreitol":[34],"0.2%":[36,81],"Tween":[37,82],"20":[38],"applied":[40,53],"to":[41,54,70,92,121,123,138,170],"a":[42,55,58,86,197,221],"WGA-Sepharose.":[43],"The":[44,116],"bound":[45,69,169,185],"fractions":[46],"eluted":[48,75],"0.3M":[50],"N-actylglucosamine,":[51],"then":[52],"TM60":[56],"(":[57],"monoclonal":[59],"antibody":[60],"against":[61],"GP":[62,67,93,102],"Ib)-Affi-gel":[63],"column.":[64],"Only":[65],"one":[66],"column":[72],"50mM":[77],"glycine-HCl,":[78],"pH3.0,":[79],"containing":[80],"20.":[83],"SDS-PAGE":[84],"showed":[85,188],"single":[87],"band":[88],"130kDa,":[90],"corresponding":[91],"alpha":[95],"chain":[96],"(GP":[97],"Ib\u03b1).":[98],"When":[99,141],"purified":[101,230],"Ib\u03b1":[103],"digested":[105],"trypsin,":[107],"two":[108],"fragments":[109,150,164],"(94kDa":[110],".":[111],"43kDa)":[113],"obtained.":[115],"43kDa":[117,226],"fragment":[118,134,178,192,228],"shown":[120],"bind":[122,137],"both":[124,171,182],"affinity":[125,199],"colomns":[126],"TM60-":[128,158],"thrombin-Affi-gel,":[130],"while":[131],"94kDa":[133],"did":[135],"not":[136],"either":[139],"Affi-gel.":[140],"same":[143],"experiment":[144],"performed":[146],"using":[147,239],"chymotrypsin,":[148],"three":[149],"(94kDa,":[151],"45kDa":[152,191],"39kDa)":[154,167],"observed.":[156],"On":[157],"thrombin-Affi-Gel":[160,175],"columns,":[161],"smaller":[163],"(45kDa":[165],"columns.":[172],"However,":[173],"on.":[174],"column,":[176],"39kDa":[177,202],"found":[180],"in":[181,271,276],"unbound":[183],"fractions.":[186],"It":[187],"that":[189,207],"interacts":[193],"thrombin":[195],"higher":[198],"than":[200],"fragment.":[203],"These":[204],"results":[205],"indicate":[206],"is":[211],"located":[212],"\"tail\"":[215],"portion":[216],"GPIba,":[218],"especially":[219],"chymotryptic":[222],"cleavage":[223],"site.":[224],"Then":[225],"tryptic":[227],"its":[232,248],"partial":[233],"amino":[234,242,249],"analyzed":[238],"gas":[240],"phase":[241],"analyzer.":[245],"Based":[246],"sequence,":[251],"several":[252],"oligopeptides":[254],"synthesized.":[256],"Three":[257],"(#1-10,":[259],"#11-23,":[260],"#17-28)":[261],"inhibited":[262],"0.05":[263],"U/ml":[264],"aggregations":[266],"washed":[268],"human":[269],"platelets":[270],"dose-dependent":[272],"manners.":[273],"IC50":[274],"range":[278],"L50-550\u03bcM":[280],"for":[281],"each":[282],"these":[284],"peptides.":[285]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2018-09-07T00:00:00"}