{"id":"https://openalex.org/W2889229694","doi":"https://doi.org/10.1055/s-0038-1643280","title":"REGULATION OF FACTOR V (FV) EXPRESSION IN HUMAN MEGAKARYOCYTES","display_name":"REGULATION OF FACTOR V (FV) EXPRESSION IN HUMAN MEGAKARYOCYTES","publication_year":1987,"publication_date":"1987-01-01","ids":{"openalex":"https://openalex.org/W2889229694","doi":"https://doi.org/10.1055/s-0038-1643280","mag":"2889229694"},"language":"en","primary_location":{"id":"doi:10.1055/s-0038-1643280","doi":"https://doi.org/10.1055/s-0038-1643280","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1643280","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109984754","display_name":"Alan M. Gewirtz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"A Gewirtz","raw_affiliation_strings":["Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066870257","display_name":"C L shepiro","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C L shepiro","raw_affiliation_strings":["Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054459036","display_name":"R Bovd","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R Bovd","raw_affiliation_strings":["Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A","institution_ids":["https://openalex.org/I84392919"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5104652394","display_name":"Robert W. Colman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I84392919","display_name":"Temple University","ror":"https://ror.org/00kx1jb78","country_code":"US","type":"education","lineage":["https://openalex.org/I84392919"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R W Colman","raw_affiliation_strings":["Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dept. of Med., and Thrombosis Res. Ctr., Temple Univ. Sch. of Med., Philadelphia, Pennsylvania, U.S.A","institution_ids":["https://openalex.org/I84392919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I84392919"],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.36879433,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.9419000148773193,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10746","display_name":"Platelet Disorders and Treatments","score":0.9419000148773193,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10881","display_name":"Blood Coagulation and Thrombosis Mechanisms","score":0.042500000447034836,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11280","display_name":"Hemophilia Treatment and Research","score":0.008299999870359898,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/factor-v","display_name":"factor V","score":0.8379999995231628},{"id":"https://openalex.org/keywords/megakaryocytes","display_name":"megakaryocytes","score":0.949999988079071},{"id":"https://openalex.org/keywords/megakaryocyte-maturation","display_name":"megakaryocyte maturation","score":0.9269999861717224},{"id":"https://openalex.org/keywords/tetradecanoyl-phorbol-acetate","display_name":"tetradecanoyl phorbol acetate","score":0.8500000238418579},{"id":"https://openalex.org/keywords/cell-size","display_name":"cell size","score":0.6420000195503235},{"id":"https://openalex.org/keywords/dna-ploidy","display_name":"DNA ploidy","score":0.890999972820282},{"id":"https://openalex.org/keywords/megakaryopoiesis","display_name":"megakaryopoiesis","score":0.8100000023841858},{"id":"https://openalex.org/keywords/megakaryocyte-proliferation","display_name":"megakaryocyte proliferation","score":0.4519999921321869},{"id":"https://openalex.org/keywords/megakaryocyte-apoptosis","display_name":"megakaryocyte apoptosis","score":0.4959999918937683}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5991010665893555},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5643254518508911},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4366067945957184},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4259258508682251},{"id":"https://openalex.org/C204628709","wikidata":"https://www.wikidata.org/wiki/Q864854","display_name":"Biotinylation","level":2,"score":0.41142353415489197},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.3325273394584656},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10868939757347107},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1055/s-0038-1643280","doi":"https://doi.org/10.1055/s-0038-1643280","is_oa":false,"landing_page_url":"https://doi.org/10.1055/s-0038-1643280","pdf_url":null,"source":{"id":"https://openalex.org/S206235894","display_name":"Thrombosis and Haemostasis","issn_l":"0340-6245","issn":["0340-6245","2567-689X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320000","host_organization_name":"Thieme Medical Publishers (Germany)","host_organization_lineage":["https://openalex.org/P4310320000"],"host_organization_lineage_names":["Thieme Medical Publishers (Germany)"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Thrombosis and Haemostasis","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2040789492","https://openalex.org/W167595690","https://openalex.org/W2021504120","https://openalex.org/W2085740929","https://openalex.org/W4253952251","https://openalex.org/W2409307674","https://openalex.org/W2404676799","https://openalex.org/W226237940","https://openalex.org/W2429600651","https://openalex.org/W2112700347"],"abstract_inverted_index":{"We":[0,316],"have":[1],"suggested":[2],"that":[3,22,321,346],"synthesis":[4,54,324,328,349],"of":[5,138,198,222,226,274,285,303,310],"FY":[6,33,36,53,74,127,169,209,247,257,311,327],"by":[7,96,104,147,187,280,355],"human":[8,88],"megakaryocytes":[9],"(MEGS),":[10],"is":[11,17,37,325],"a":[12,135,151,160,220],"maturation":[13,71,80,213,277],"relrted":[14],"event.":[15],"This":[16],"based":[18],"on":[19,123],"our":[20],"finding":[21],"when":[23,244],"MEGS":[24,56,89,304],"are":[25,57,62,339],"cloned":[26],"from":[27,92,318],"progenitor":[28],"cells":[29,45,112],"in":[30,32,41,55,109,134,143,189,219,251,353],"vitro":[31],"depleted":[34],"medium,":[35],"immunochemically":[38],"detectable":[39],"only":[40],"morphologically":[42],"recognizable":[43],"(mature)":[44],"(Blood":[46],"67:1639,":[47],"'86).":[48],"The":[49],"stimuli":[50],"which":[51,68],"induce":[52],"unknown,":[58],"but":[59],"if":[60],"they":[61],"related":[63],"or":[64,216],"linked":[65],"to":[66,181,204,270,332],"factors":[67],"regulate":[69],"terminal":[70],"processes":[72],"cellular":[73],"levels":[75,130,258],"might":[76],"increase":[77],"as":[78,278],"the":[79,139,167,190,227,301,308,356],"routine":[81],"proceeds.":[82],"To":[83],"test":[84],"this":[85],"hypothesis,":[86],"mature":[87,275],"were":[90,113,175,201],"isolated":[91],"normal":[93],"bone":[94],"marrow":[95],"counterflow":[97],"centrifugel":[98],"elutrietion,":[99],"deposited":[100],"onto":[101],"gloss":[102],"slides":[103],"cytocentri-fugation,":[105],"and":[106,159,196,211,229,264,266,307,329,336],"then":[107,114,176],"fixed":[108],"methanol:acetone.":[110],"Individual":[111],"staged,":[115],"geometric":[116],"meon":[117],"cell":[118,262,334],"diometer":[119],"(size)":[120],"determined":[121,279],"with":[122,178,261,272],"opticol":[124],"fylor,":[125],"end":[126,128],"DNA":[129,185],"measured.":[131],"FV":[132,233,323,348],"expression":[133],"large":[136],"area":[137],"MEG":[140,212,232,246,256,276,347],"(38":[141],"urn":[142],"diameter)":[144],"was":[145,249],"semi-quantitated":[146],"microspectrophotometry":[148],"(MSPEC)":[149],"using":[150],"sensitive":[152],"Texas":[153],"Red":[154],"labeled":[155],"streptavidin-biotin":[156],"detection":[157],"system":[158],"monoclonal":[161],"antibody":[162],"probe":[163],"(B10)":[164],"directed":[165],"against":[166],"non-binding":[168],"activation":[170],"peptide":[171],"(150,000":[172],"kd).":[173],"Cells":[174],"reacted":[177],"Chromomycin":[179],"A3":[180],"allow":[182],"for":[183],"simultaneous":[184],"quantitation":[186],"MSPEC":[188],"same":[191],"cell.":[192,315],"Correlation":[193],"coefficients":[194],"(r)":[195],"coefficient":[197],"determination":[199],"[r2]":[200],"computer":[202],"generated":[203],"discern":[205],"potential":[206],"relationships":[207],"between":[208],"expression,":[210],"stage,":[214],"size":[215,263,335],"ploidy":[217,337],"level":[218,309],"total":[221,245],"532":[223],"MEGS.":[224,254],"Characteristics":[225],"population,":[228],"r/r2":[230,238],"vs.":[231],"content":[234,248],"ere":[235],"shown":[236],"below:":[237],"values":[239],"did":[240,267],"not":[241,268],"significantly":[242],"change":[243],"measured":[250],"100":[252],"additional":[253],"Therefore,":[255],"varied":[259],"independently":[260],"ploidy,":[265],"appear":[269],"correlate":[271],"degree":[273],"standard":[281],"critera.":[282],"In":[283],"two":[284],"four":[286],"preliminary":[287],"experiments,":[288],"low":[289],"dose":[290],"(8nM":[291],"ql2h":[292],"\u00d7":[293],"4":[294],"doses)":[295],"tetradecanoyl":[296],"phorbol":[297],"acetate":[298],"augmented":[299],"both":[300],"number":[302],"expressing":[305],"FV,":[306],"expressed":[312],"per":[313],"individual":[314],"conclude":[317],"these":[319],"data":[320,343],"once":[322],"initiated,":[326],"events":[330],"relating":[331],"final":[333],"development":[338],"regulated":[340,352],"independently.":[341],"Our":[342],"also":[344],"suggest":[345],"may":[350],"be":[351],"part":[354],"phosphoinositide-protein":[357],"kinase":[358],"C":[359],"system.":[360]},"counts_by_year":[],"updated_date":"2026-07-29T14:22:42.915294","created_date":"2018-09-07T00:00:00"}