{"id":"https://openalex.org/W2888598366","doi":"https://doi.org/10.1021/acssynbio.8b00260","title":"A Robust and Quantitative Reporter System To Evaluate Noncanonical Amino Acid Incorporation in Yeast","display_name":"A Robust and Quantitative Reporter System To Evaluate Noncanonical Amino Acid Incorporation in Yeast","publication_year":2018,"publication_date":"2018-08-23","ids":{"openalex":"https://openalex.org/W2888598366","doi":"https://doi.org/10.1021/acssynbio.8b00260","mag":"2888598366","pmid":"https://pubmed.ncbi.nlm.nih.gov/30139255","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6214617"},"language":"en","primary_location":{"id":"doi:10.1021/acssynbio.8b00260","doi":"https://doi.org/10.1021/acssynbio.8b00260","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssynbio.8b00260","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6214617","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5059232605","display_name":"Jessica T. Stieglitz","orcid":"https://orcid.org/0000-0003-3845-0712","observed_orcids":["https://orcid.org/0000-0003-3845-0712"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica T. Stieglitz","raw_affiliation_strings":["Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0003-3845-0712","affiliations":[{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050344968","display_name":"Haixing P. Kehoe","orcid":"https://orcid.org/0000-0002-3598-3700","observed_orcids":["https://orcid.org/0000-0002-3598-3700"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haixing P. Kehoe","raw_affiliation_strings":["Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0002-3598-3700","affiliations":[{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5071648598","display_name":"Ming Lei","orcid":"https://orcid.org/0000-0002-0275-5605","observed_orcids":["https://orcid.org/0000-0002-0275-5605"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ming Lei","raw_affiliation_strings":["Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0002-0275-5605","affiliations":[{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062531357","display_name":"James A. Van Deventer","orcid":"https://orcid.org/0000-0003-4343-6157","observed_orcids":["https://orcid.org/0000-0003-4343-6157"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James A. Van Deventer","raw_affiliation_strings":["Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States"],"raw_orcid":"https://orcid.org/0000-0003-4343-6157","affiliations":[{"raw_affiliation_string":"Biomedical Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Chemical and Biological Engineering Department, Tufts University, Medford, Massachusetts 02155, United States","institution_ids":["https://openalex.org/I121934306"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5062531357"],"corresponding_institution_ids":["https://openalex.org/I121934306"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.9731,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.73746501,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"7","issue":"9","first_page":"2256","last_page":"2269"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/amber-codon-suppression","display_name":"amber codon suppression","score":0.968999981880188},{"id":"https://openalex.org/keywords/orthogonal-translation-systems","display_name":"orthogonal translation systems","score":0.8190000057220459},{"id":"https://openalex.org/keywords/antibody-fragments","display_name":"antibody fragments","score":0.4169999957084656},{"id":"https://openalex.org/keywords/leucyl-trna-synthetase","display_name":"leucyl-tRNA synthetase","score":0.8999999761581421},{"id":"https://openalex.org/keywords/genetic-code-expansion","display_name":"genetic code expansion","score":0.6890000104904175},{"id":"https://openalex.org/keywords/stop-codon-suppression","display_name":"stop codon suppression","score":0.7310000061988831},{"id":"https://openalex.org/keywords/single-cell-analysis","display_name":"single-cell analysis","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C10853874","wikidata":"https://www.wikidata.org/wiki/Q180618","display_name":"Genetic code","level":3,"score":0.7354245781898499},{"id":"https://openalex.org/C20580545","wikidata":"https://www.wikidata.org/wiki/Q28354","display_name":"Stop codon","level":3,"score":0.7106457352638245},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.629929780960083},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.6073386669158936},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.5538173317909241},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.5488888621330261},{"id":"https://openalex.org/C2779315393","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Aminoacyl tRNA synthetase","level":5,"score":0.5319505333900452},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5093094110488892},{"id":"https://openalex.org/C2910418148","wikidata":"https://www.wikidata.org/wiki/Q415968","display_name":"Amino Acyl-tRNA Synthetases","level":5,"score":0.5044988393783569},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.48311519622802734},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.46726933121681213},{"id":"https://openalex.org/C191908910","wikidata":"https://www.wikidata.org/wiki/Q862838","display_name":"Synthetic biology","level":2,"score":0.4669220447540283},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.44815242290496826},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3931884169578552},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.31706517934799194},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.25978899002075195},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.22803980112075806},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2060033082962036},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1562524139881134}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000604","descriptor_name":"Amino Acyl-tRNA Synthetases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012343","descriptor_name":"RNA, Transfer","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018388","descriptor_name":"Codon, Terminator","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1021/acssynbio.8b00260","doi":"https://doi.org/10.1021/acssynbio.8b00260","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssynbio.8b00260","pdf_url":null,"source":{"id":"https://openalex.org/S54167817","display_name":"ACS Synthetic Biology","issn_l":"2161-5063","issn":["2161-5063"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Synthetic Biology","raw_type":"journal-article"},{"id":"pmid:30139255","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30139255","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS synthetic biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6214617","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6214617","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"ACS Synth Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6214617","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6214617","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"ACS Synth Biol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1059077631","display_name":null,"funder_award_id":"2016231237","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G529895842","display_name":null,"funder_award_id":"W911NF-16-1-0175","funder_id":"https://openalex.org/F4320338281","funder_display_name":"Army Research Office"},{"id":"https://openalex.org/G5313451042","display_name":"A Machine-Learning Based Software Widget for Resolving Metabolite Identities","funder_award_id":"R03CA211839","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320310245","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320338281","display_name":"Army Research Office","ror":"https://ror.org/05epdh915"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":75,"referenced_works":["https://openalex.org/W914903399","https://openalex.org/W1965347340","https://openalex.org/W1966287798","https://openalex.org/W1971596340","https://openalex.org/W1973268713","https://openalex.org/W1976230851","https://openalex.org/W1978858109","https://openalex.org/W1978880815","https://openalex.org/W1981156768","https://openalex.org/W1983754329","https://openalex.org/W1985650731","https://openalex.org/W1986561802","https://openalex.org/W1987056309","https://openalex.org/W1990608083","https://openalex.org/W1990789777","https://openalex.org/W1992502609","https://openalex.org/W1996935079","https://openalex.org/W2004411392","https://openalex.org/W2011218110","https://openalex.org/W2013321787","https://openalex.org/W2019670700","https://openalex.org/W2020030845","https://openalex.org/W2024156903","https://openalex.org/W2036078885","https://openalex.org/W2039295326","https://openalex.org/W2041292513","https://openalex.org/W2048452070","https://openalex.org/W2057434657","https://openalex.org/W2060391020","https://openalex.org/W2062660287","https://openalex.org/W2065600580","https://openalex.org/W2074244626","https://openalex.org/W2077148282","https://openalex.org/W2082845995","https://openalex.org/W2089268815","https://openalex.org/W2091512138","https://openalex.org/W2092846883","https://openalex.org/W2093040180","https://openalex.org/W2094654556","https://openalex.org/W2094688585","https://openalex.org/W2101358681","https://openalex.org/W2102755926","https://openalex.org/W2107167859","https://openalex.org/W2110062500","https://openalex.org/W2126576163","https://openalex.org/W2128660010","https://openalex.org/W2138750321","https://openalex.org/W2140665471","https://openalex.org/W2146913917","https://openalex.org/W2148313902","https://openalex.org/W2163266294","https://openalex.org/W2165286214","https://openalex.org/W2172121653","https://openalex.org/W2193229373","https://openalex.org/W2197946614","https://openalex.org/W2261688117","https://openalex.org/W2270783611","https://openalex.org/W2332024781","https://openalex.org/W2412132105","https://openalex.org/W2499777615","https://openalex.org/W2513614261","https://openalex.org/W2522501918","https://openalex.org/W2548431358","https://openalex.org/W2562682295","https://openalex.org/W2606822983","https://openalex.org/W2762868004","https://openalex.org/W2763278454","https://openalex.org/W2767673635","https://openalex.org/W2769554577","https://openalex.org/W2783185708","https://openalex.org/W2790405891","https://openalex.org/W2802984935","https://openalex.org/W2806189034","https://openalex.org/W2969596202","https://openalex.org/W4231832388"],"related_works":["https://openalex.org/W3001997065","https://openalex.org/W4308531248","https://openalex.org/W4284894834","https://openalex.org/W3135431829","https://openalex.org/W2604888580","https://openalex.org/W4382344905","https://openalex.org/W2522501918","https://openalex.org/W4399935898","https://openalex.org/W6950542240","https://openalex.org/W2061541382"],"abstract_inverted_index":{"Engineering":[0],"protein":[1],"translation":[2,77],"machinery":[3],"to":[4,18,31,42,48,131,160,185,204,244],"incorporate":[5,132],"noncanonical":[6],"amino":[7],"acids":[8],"(ncAAs)":[9],"into":[10],"proteins":[11,236],"has":[12],"advanced":[13],"applications":[14,22,238],"ranging":[15,239],"from":[16,127,240],"proteomics":[17],"single-molecule":[19],"studies.":[20],"As":[21],"of":[23,94,146,149,156,189,198,218,231,248],"ncAAs":[24],"emerge,":[25],"efficient":[26,144,192],"ncAA":[27,44,70],"incorporation":[28,45,145],"is":[29,71,80,183],"crucial":[30],"exploiting":[32],"unique":[33],"chemistries.":[34],"We":[35],"have":[36],"established":[37],"a":[38,69,147,161],"quantitative":[39,187],"reporter":[40,58],"platform":[41,182],"evaluate":[43],"in":[46,53,210,235],"response":[47],"the":[49,74,111,220,229],"TAG":[50],"(amber)":[51],"codon":[52,66,113,194,201],"yeast.":[54],"This":[55,181],"yeast":[56],"display-based":[57],"utilizes":[59],"an":[60,64],"antibody":[61],"fragment":[62],"containing":[63],"amber":[65,108,112],"at":[67,84],"which":[68],"incorporated":[72],"when":[73],"appropriate":[75],"orthogonal":[76],"system":[78],"(OTS)":[79],"present.":[81],"Epitope":[82],"tags":[83],"both":[85],"termini":[86],"allow":[87,186],"for":[88,237],"flow":[89],"cytometry-based":[90],"end":[91],"point":[92],"readouts":[93,176],"OTS":[95],"efficiency":[96],"and":[97,115,151,196],"fidelity.":[98],"Using":[99],"this":[100],"reporter,":[101,164],"we":[102,152],"evaluated":[103],"several":[104,157],"factors":[105],"that":[106,125],"influence":[107],"suppression,":[109],"including":[110],"position":[114],"different":[116,128,136],"aminoacyl-tRNA":[117],"synthetase/tRNA":[118],"(aaRS/tRNA)":[119],"pairs.":[120],"Interestingly,":[121],"previously":[122],"described":[123],"aaRSs":[124],"evolved":[126,226],"parent":[129],"enzymes":[130],"O-methyl-l-tyrosine":[133],"exhibit":[134],"vastly":[135],"behavior.":[137],"Escherichia":[138],"coli":[139],"leucyl-tRNA":[140],"synthetase":[141],"variants":[142],"demonstrated":[143],"range":[148,230],"ncAAs,":[150],"discovered":[153],"unreported":[154],"activities":[155],"variants.":[158],"Compared":[159],"plate":[162],"reader-based":[163],"our":[165,216],"assay":[166],"yields":[167],"more":[168],"precise":[169],"bulk-level":[170],"measurements":[171],"while":[172,227],"also":[173],"supporting":[174],"single-cell":[175],"compatible":[177],"with":[178],"cell":[179],"sorting.":[180],"expected":[184],"elucidation":[188],"principles":[190],"dictating":[191],"stop":[193,200],"suppression":[195,202],"evolution":[197],"next-generation":[199],"systems":[203],"further":[205,225],"enhance":[206],"genetic":[207,221],"code":[208,222],"manipulation":[209],"eukaryotes.":[211],"These":[212],"efforts":[213],"will":[214],"improve":[215],"understanding":[217],"how":[219],"can":[223],"be":[224],"expanding":[228],"chemical":[232],"diversity":[233],"available":[234],"fundamental":[241],"epigenetics":[242],"studies":[243],"engineering":[245],"new":[246],"classes":[247],"therapeutics.":[249]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":11},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":12},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2016,"cited_by_count":1}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2018-08-31T00:00:00"}
