{"id":"https://openalex.org/W2887543326","doi":"https://doi.org/10.1158/1538-7445.am2018-862","title":"Abstract 862: Effects of fixation and antigen retrieval on the RNA integrity number","display_name":"Abstract 862: Effects of fixation and antigen retrieval on the RNA integrity number","publication_year":2018,"publication_date":"2018-07-01","ids":{"openalex":"https://openalex.org/W2887543326","doi":"https://doi.org/10.1158/1538-7445.am2018-862","mag":"2887543326"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2018-862","doi":"https://doi.org/10.1158/1538-7445.am2018-862","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2018-862","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038977436","display_name":"Dennis Otali","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dennis Otali","raw_affiliation_strings":["1Univ. of Alabama at Birmingham, Birmingham, AL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Univ. of Alabama at Birmingham, Birmingham, AL;","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036423966","display_name":"Maitlyn Mullen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I83321563","display_name":"Samford University","ror":"https://ror.org/0040cz635","country_code":"US","type":"education","lineage":["https://openalex.org/I83321563"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Maitlyn Mullen","raw_affiliation_strings":["2Samford University, Birmingham, AL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2Samford University, Birmingham, AL;","institution_ids":["https://openalex.org/I83321563"]}]},{"author_position":"middle","author":{"id":null,"display_name":"He Qinghua","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I82497590","display_name":"Auburn University","ror":"https://ror.org/02v80fc35","country_code":"US","type":"education","lineage":["https://openalex.org/I82497590"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"He Qinghua","raw_affiliation_strings":["3Auburn University, Auburn, AL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3Auburn University, Auburn, AL","institution_ids":["https://openalex.org/I82497590"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057213757","display_name":"Denise K. Oelschlager","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Denise Oelschlager","raw_affiliation_strings":["1Univ. of Alabama at Birmingham, Birmingham, AL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Univ. of Alabama at Birmingham, Birmingham, AL;","institution_ids":["https://openalex.org/I32389192"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5076077003","display_name":"William E. Grizzle","orcid":"https://orcid.org/0000-0001-9245-0401","observed_orcids":["https://orcid.org/0000-0001-9245-0401"]},"institutions":[{"id":"https://openalex.org/I32389192","display_name":"University of Alabama at Birmingham","ror":"https://ror.org/008s83205","country_code":"US","type":"education","lineage":["https://openalex.org/I32389192"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"William E. Grizzle","raw_affiliation_strings":["1Univ. of Alabama at Birmingham, Birmingham, AL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Univ. of Alabama at Birmingham, Birmingham, AL;","institution_ids":["https://openalex.org/I32389192"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"78","issue":"13_Supplement","first_page":"862","last_page":"862"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10885","display_name":"Gene expression and cancer classification","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rin","display_name":"RNA integrity number","score":0.9879999756813049},{"id":"https://openalex.org/keywords/formalin-fixation","display_name":"formalin fixation","score":0.7839999794960022},{"id":"https://openalex.org/keywords/antigen-retrieval","display_name":"antigen retrieval","score":0.968999981880188},{"id":"https://openalex.org/keywords/fixation-duration","display_name":"fixation duration","score":0.5809999704360962},{"id":"https://openalex.org/keywords/formaldehyde-crosslinking","display_name":"formaldehyde crosslinking","score":0.6899999976158142},{"id":"https://openalex.org/keywords/rna-degradation","display_name":"RNA degradation","score":0.625},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.878000020980835},{"id":"https://openalex.org/keywords/mrna-stability","display_name":"mRNA stability","score":0.5550000071525574},{"id":"https://openalex.org/keywords/cancer-cell-lines","display_name":"cancer cell lines","score":0.7450000047683716},{"id":"https://openalex.org/keywords/prostate-cancer","display_name":"prostate cancer","score":0.5720000267028809},{"id":"https://openalex.org/keywords/breast-cancer","display_name":"breast cancer","score":0.9990000128746033},{"id":"https://openalex.org/keywords/pancreatic-cancer","display_name":"pancreatic cancer","score":0.9369999766349792},{"id":"https://openalex.org/keywords/rt-qpcr","display_name":"RT-qPCR","score":0.37599998712539673},{"id":"https://openalex.org/keywords/agilent-2100-bioanalyzer","display_name":"Agilent 2100 Bioanalyzer","score":0.8880000114440918},{"id":"https://openalex.org/keywords/ribosomal-rna","display_name":"ribosomal RNA","score":0.39800000190734863}],"concepts":[{"id":"https://openalex.org/C2779484032","wikidata":"https://www.wikidata.org/wiki/Q25098668","display_name":"Antigen retrieval","level":3,"score":0.8247547149658203},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.7162684202194214},{"id":"https://openalex.org/C146249460","wikidata":"https://www.wikidata.org/wiki/Q2914991","display_name":"Fixation (population genetics)","level":3,"score":0.5975161194801331},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.5314176678657532},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.47076988220214844},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.47010013461112976},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.45028501749038696},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.4307720959186554},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42572274804115295},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3862173557281494},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.35063809156417847},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.20835742354393005},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1987549066543579},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11395370960235596},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.11326876282691956}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2018-862","doi":"https://doi.org/10.1158/1538-7445.am2018-862","is_oa":false,"landing_page_url":"https://doi.org/10.1158/1538-7445.am2018-862","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.504800021648407}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2047355957","https://openalex.org/W7096182097","https://openalex.org/W2137530145","https://openalex.org/W880729474","https://openalex.org/W2149960285","https://openalex.org/W7095084251","https://openalex.org/W2774646088","https://openalex.org/W2076475433","https://openalex.org/W2107061369","https://openalex.org/W1992425156"],"abstract_inverted_index":{"Abstract":[0],"The":[1,187,226,260],"effect":[2],"of":[3,33,41,44,52,80,99,105,117,128,145,183,196,235,237,268,370,393,410,419,441,454],"fixation":[4,92,236,248,291,303,346,369,394,442],"and":[5,47,87,149,165,212,224,255,280,290,297,308,314,349,398,443],"antigen":[6,111,444],"retrieval":[7,112,445],"(AR)":[8,113],"on":[9,272,285,446],"RNA":[10,15,46,164,311,420,448],"as":[11,140,190],"measured":[12,169],"by":[13],"the":[14,42,103,142,194,217,233,266,286,318,326,336,380,404,447,455],"integrity":[16,449],"number":[17,450],"(RIN)":[18],"has":[19],"not":[20,48,388,401,415],"been":[21],"adequately":[22],"explored.":[23],"RIN":[24,36,54,181,197,223,315,322,343,376,386,399,413],"is":[25,37,131,193,414],"an":[26,38,49],"index":[27],"(pseudo-measure)":[28],"used":[29,123,203,284],"to":[30,62,83,124,191,215,230,264,339,377],"measure":[31,40,51,418],"stability":[32,43,421],"mRNA;":[34],"however,":[35],"actual":[39,50],"ribosomal":[45],"mRNA.":[53],"values":[55,182,323,344,406],"range":[56],"from":[57,325,403],"1":[58],"for":[59,64,152,355,383,458],"highly":[60],"degraded":[61],"10":[63],"intact":[65,146],"RNA.":[66],"Most":[67],"surgical":[68],"specimens":[69,138],"are":[70,122,352,361],"fixed":[71,172,384,405,425],"in":[72,95,170,198,239,245,342,372,395,422],"10%":[73,220,240,373,396,423],"neutral":[74],"buffered":[75],"formalin":[76,171],"(3.7%":[77],"formaldehyde)":[78],"because":[79],"its":[81],"ability":[82],"preserve":[84],"morphological":[85],"detail":[86],"prevent":[88],"degradation.":[89],"However,":[90,155],"formaldehyde":[91],"causes":[93],"changes":[94],"proteins":[96],"through":[97],"formation":[98],"crosslinks,":[100],"which":[101,178],"masks":[102],"immunorecognition":[104],"some":[106],"antigens.":[107],"In":[108],"immunohistochemical":[109],"evaluations,":[110],"approaches":[114,271],"e.g.,":[115],"boiling":[116],"tissue":[118,176],"sections":[119],"under":[120],"pressure":[121],"reverse":[125],"these":[126],"effects":[127,267],"fixation.":[129],"It":[130],"believed":[132],"that":[133,163,335,368],"fresh":[134],"or":[135,185,302],"snap":[136],"frozen":[137],"serve":[139],"most":[141],"suitable":[143],"source":[144],"mRNA,":[147],"DNA":[148,166],"other":[150],"macromolecules":[151],"molecular":[153],"analysis.":[154],"more":[156],"recent":[157],"studies":[158],"using":[159,317],"rt-Q":[160],"PCR":[161],"indicate":[162],"can":[167],"be":[168],"paraffin":[173],"embedded":[174],"(FFPE)":[175],"samples":[177],"usually":[179],"have":[180],"3":[184,341,379],"less.":[186],"question":[188],"arises":[189],"what":[192],"usefulness":[195],"FFPE":[199],"tissues.":[200,385],"This":[201,365],"study":[202,366],"three":[204],"cancer":[205],"cell":[206,288,295,357],"lines":[207,289],"DU145":[208],"(prostate),":[209],"MDA231":[210],"(breast)":[211],"S2VP10":[213],"(pancreas)":[214],"examine":[216],"relationship":[218],"between":[219],"NBF":[221,241,374,397,424],"fixation,":[222,301],"AR.":[225,411],"first":[227],"objective":[228,262],"was":[229,263,312],"analyze":[231],"whether":[232],"duration":[234],"cells":[238,371],"correlated":[242],"with":[243,391],"decreases":[244,338],"RIN.":[246,273],"Three":[247],"times":[249,306],"i.e.,":[250,299],"5":[251],"minutes,":[252],"20":[253,348],"minutes":[254,257],"120":[256,350],"were":[258,283,330],"studied.":[259],"second":[261],"investigate":[265],"two":[269],"AR":[270,275],"Two":[274],"conditions":[276],"at":[277,304],"pH":[278,281],"6":[279],"9":[282],"same":[287],"times.":[292],"For":[293],"each":[294],"line":[296],"treatment":[298],"no":[300],"different":[305],"(with":[307],"without":[309],"AR),":[310],"extracted":[313],"analyzed":[316],"Agilent":[319,327],"2100":[320,328],"Bioanalyzer.":[321],"obtained":[324],"Bioanalyzer":[329],"statistically":[331,353],"analyzed.":[332],"T-tests":[333],"show":[334],"large":[337],"about":[340,378],"after":[345,407],"(5,":[347],"minutes)":[351],"significant":[354],"all":[356],"lines.":[358],"All":[359],"p-values":[360],"less":[362],"than":[363],"0.0001.":[364],"confirms":[367],"lowers":[375],"value":[381],"observed":[382],"did":[387,400],"vary":[389],"significantly":[390],"time":[392],"change":[402],"either":[408],"condition":[409],"Thus":[412],"a":[416],"useful":[417],"tissue.":[426],"Citation":[427],"Format:":[428],"Dennis":[429],"Otali,":[430],"Maitlyn":[431],"Mullen,":[432],"He":[433],"Qinghua,":[434],"Denise":[435],"Oelschlager,":[436],"William":[437],"E.":[438],"Grizzle.":[439],"Effects":[440],"[abstract].":[451],"In:":[452],"Proceedings":[453],"American":[456],"Association":[457],"Cancer":[459,472],"Research":[460],"Annual":[461],"Meeting":[462],"2018;":[463],"2018":[464],"Apr":[465],"14-18;":[466],"Chicago,":[467],"IL.":[468],"Philadelphia":[469],"(PA):":[470],"AACR;":[471],"Res":[473],"2018;78(13":[474],"Suppl):Abstract":[475],"nr":[476],"862.":[477]},"counts_by_year":[],"updated_date":"2026-09-27T08:07:27.782135","created_date":"2018-08-22T00:00:00"}
