{"id":"https://openalex.org/W2887106926","doi":"https://doi.org/10.1161/circ.132.suppl_3.19973","title":"Abstract 19973: Decellularized Extracellular Matrix Exhibits Reduced Stiffness and Immature Collagen Deposition in a Mouse Model of Left Heart Failure","display_name":"Abstract 19973: Decellularized Extracellular Matrix Exhibits Reduced Stiffness and Immature Collagen Deposition in a Mouse Model of Left Heart Failure","publication_year":2015,"publication_date":"2015-11-10","ids":{"openalex":"https://openalex.org/W2887106926","doi":"https://doi.org/10.1161/circ.132.suppl_3.19973","mag":"2887106926"},"language":"en","primary_location":{"id":"doi:10.1161/circ.132.suppl_3.19973","doi":"https://doi.org/10.1161/circ.132.suppl_3.19973","is_oa":false,"landing_page_url":"https://doi.org/10.1161/circ.132.suppl_3.19973","pdf_url":null,"source":{"id":"https://openalex.org/S116251202","display_name":"Circulation","issn_l":"0009-7322","issn":["0009-7322","1524-4539"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5084212280","display_name":"Kevin Morine","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1290900354","display_name":"Tufts Medical Center","ror":"https://ror.org/002hsbm82","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1290900354"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kevin Morine","raw_affiliation_strings":["Cardiology, Tufts Med Cntr MCRI, Boston, MA","Kevin Morine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiology, Tufts Med Cntr MCRI, Boston, MA","institution_ids":["https://openalex.org/I1290900354"]},{"raw_affiliation_string":"Kevin Morine","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065943008","display_name":"Kyle P. Quinn","orcid":"https://orcid.org/0000-0002-6876-3608","observed_orcids":["https://orcid.org/0000-0002-6876-3608"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kyle Quinn","raw_affiliation_strings":["Biomedical Engineering, Tufts Univ, Boston, MA","Kyle Quinn"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering, Tufts Univ, Boston, MA","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Kyle Quinn","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072319946","display_name":"Lauren M. Baugh","orcid":"https://orcid.org/0000-0002-0029-5318","observed_orcids":["https://orcid.org/0000-0002-0029-5318"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren Baugh","raw_affiliation_strings":["Biomedical Engineering, Tufts Univ, Boston, MA","Lauren Baugh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering, Tufts Univ, Boston, MA","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Lauren Baugh","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081332186","display_name":"Mark A. Aronovitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1290900354","display_name":"Tufts Medical Center","ror":"https://ror.org/002hsbm82","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1290900354"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mark Aronovitz","raw_affiliation_strings":["Cardiology, Tufts Med Cntr MCRI, Boston, MA","Mark Aronovitz"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiology, Tufts Med Cntr MCRI, Boston, MA","institution_ids":["https://openalex.org/I1290900354"]},{"raw_affiliation_string":"Mark Aronovitz","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079542108","display_name":"Irene Georgakoudi","orcid":"https://orcid.org/0000-0002-0049-6697","observed_orcids":["https://orcid.org/0000-0002-0049-6697"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Irene Georgakoudi","raw_affiliation_strings":["Biomedical Engineering, Tufts Univ, Boston, MA","Irene Georgakoudi"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering, Tufts Univ, Boston, MA","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Irene Georgakoudi","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102904713","display_name":"Richard H. Karas","orcid":"https://orcid.org/0000-0001-7799-091X","observed_orcids":["https://orcid.org/0000-0001-7799-091X"]},"institutions":[{"id":"https://openalex.org/I1290900354","display_name":"Tufts Medical Center","ror":"https://ror.org/002hsbm82","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1290900354"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard H Karas","raw_affiliation_strings":["Cardiology, Tufts Med Cntr MCRI, Boston, MA","Richard H Karas"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiology, Tufts Med Cntr MCRI, Boston, MA","institution_ids":["https://openalex.org/I1290900354"]},{"raw_affiliation_string":"Richard H Karas","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038610918","display_name":"Lauren Deems Black","orcid":"https://orcid.org/0000-0002-7486-5811","observed_orcids":["https://orcid.org/0000-0002-7486-5811"]},"institutions":[{"id":"https://openalex.org/I121934306","display_name":"Tufts University","ror":"https://ror.org/05wvpxv85","country_code":"US","type":"education","lineage":["https://openalex.org/I121934306"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lauren Black","raw_affiliation_strings":["Biomedical Engineering, Tufts Univ, Boston, MA","Lauren Black"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering, Tufts Univ, Boston, MA","institution_ids":["https://openalex.org/I121934306"]},{"raw_affiliation_string":"Lauren Black","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5000653485","display_name":"Navin Kumar Kapur","orcid":"https://orcid.org/0000-0002-8302-6796","observed_orcids":["https://orcid.org/0000-0002-8302-6796"]},"institutions":[{"id":"https://openalex.org/I1290900354","display_name":"Tufts Medical Center","ror":"https://ror.org/002hsbm82","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1290900354"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Navin Kapur","raw_affiliation_strings":["Cardiology, Tufts Med Cntr MCRI, Boston, MA","Navin Kapur"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cardiology, Tufts Med Cntr MCRI, Boston, MA","institution_ids":["https://openalex.org/I1290900354"]},{"raw_affiliation_string":"Navin Kapur","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"132","issue":"suppl_3","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.8432999849319458,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.8432999849319458,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11962","display_name":"Cardiac Fibrosis and Remodeling","score":0.12229999899864197,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.012600000016391277,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/left-heart-failure","display_name":"left heart failure","score":0.6970000267028809},{"id":"https://openalex.org/keywords/decellularized-extracellular-matrix","display_name":"decellularized extracellular matrix","score":0.8859999775886536},{"id":"https://openalex.org/keywords/myocardial-stiffness","display_name":"myocardial stiffness","score":0.8539999723434448},{"id":"https://openalex.org/keywords/collagen-cross-linking","display_name":"collagen cross-linking","score":0.921999990940094},{"id":"https://openalex.org/keywords/transverse-aortic-constriction","display_name":"transverse aortic constriction","score":0.8320000171661377},{"id":"https://openalex.org/keywords/myocardial-fibrosis","display_name":"myocardial fibrosis","score":0.7549999952316284},{"id":"https://openalex.org/keywords/type-i-collagen","display_name":"type I collagen","score":0.800000011920929},{"id":"https://openalex.org/keywords/collagen-fiber-organization","display_name":"collagen fiber organization","score":0.7910000085830688},{"id":"https://openalex.org/keywords/second-harmonic-generation","display_name":"second harmonic generation","score":0.8109999895095825},{"id":"https://openalex.org/keywords/two-photon-fluorescence","display_name":"two-photon fluorescence","score":0.6740000247955322},{"id":"https://openalex.org/keywords/myocardial-mechanics","display_name":"myocardial mechanics","score":0.6190000176429749},{"id":"https://openalex.org/keywords/elastic-modulus","display_name":"elastic modulus","score":0.7509999871253967}],"concepts":[{"id":"https://openalex.org/C190672674","wikidata":"https://www.wikidata.org/wiki/Q3866591","display_name":"Decellularization","level":3,"score":0.8354318737983704},{"id":"https://openalex.org/C189165786","wikidata":"https://www.wikidata.org/wiki/Q193825","display_name":"Extracellular matrix","level":2,"score":0.741970419883728},{"id":"https://openalex.org/C2779770810","wikidata":"https://www.wikidata.org/wiki/Q23069155","display_name":"Sirius Red","level":3,"score":0.6209020018577576},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5307652354240417},{"id":"https://openalex.org/C2778198053","wikidata":"https://www.wikidata.org/wiki/Q181754","display_name":"Heart failure","level":2,"score":0.5300400257110596},{"id":"https://openalex.org/C2780644872","wikidata":"https://www.wikidata.org/wiki/Q14881714","display_name":"Type I collagen","level":2,"score":0.5257628560066223},{"id":"https://openalex.org/C2779869378","wikidata":"https://www.wikidata.org/wiki/Q409747","display_name":"Elastin","level":2,"score":0.512225329875946},{"id":"https://openalex.org/C2993866533","wikidata":"https://www.wikidata.org/wiki/Q26868","display_name":"Collagen fiber","level":2,"score":0.49309346079826355},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4325883686542511},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.37438052892684937},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.36273616552352905},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.3451384902000427},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.33047372102737427},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.25773078203201294},{"id":"https://openalex.org/C2780559512","wikidata":"https://www.wikidata.org/wiki/Q605709","display_name":"Fibrosis","level":2,"score":0.24977949261665344},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.13139018416404724}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1161/circ.132.suppl_3.19973","doi":"https://doi.org/10.1161/circ.132.suppl_3.19973","is_oa":false,"landing_page_url":"https://doi.org/10.1161/circ.132.suppl_3.19973","pdf_url":null,"source":{"id":"https://openalex.org/S116251202","display_name":"Circulation","issn_l":"0009-7322","issn":["0009-7322","1524-4539"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Circulation","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9537000060081482,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2549332164","https://openalex.org/W2283620037","https://openalex.org/W2886395580","https://openalex.org/W1966596110","https://openalex.org/W2023335850","https://openalex.org/W2050630020","https://openalex.org/W3094370103","https://openalex.org/W3122536846","https://openalex.org/W2152832086","https://openalex.org/W2318399695"],"abstract_inverted_index":{"The":[0],"extracellular":[1],"matrix":[2],"(ECM)":[3],"is":[4,231],"a":[5,27,37,155,240],"major":[6],"determinant":[7],"of":[8,33,40,54,144,236,243,267,274,282],"myocardial":[9],"mechanics":[10],"in":[11,36,104,167,197,212,239],"heart":[12,285],"failure.":[13,245,286],"Existing":[14],"methods":[15],"to":[16,25,51,61,68,157,184,191],"evaluate":[17],"ECM":[18,35,214,238,259,276],"composition":[19,260],"are":[20],"limited.":[21],"Our":[22],"objective":[23],"was":[24,102,165,182,195,218],"perform":[26],"non-destructive":[28],"microstructural":[29],"and":[30,45,59,75,87,111,116,123,137,253],"mechanical":[31,208,234],"analysis":[32,235,261],"de-cellularized":[34],"mouse":[38],"model":[39,242],"left":[41],"ventricular":[42],"(LV)":[43],"failure.Methods":[44],"Results:":[46],"Wild-type":[47],"mice":[48,71,106,169,199],"were":[49,119,139],"subjected":[50],"8":[52],"weeks":[53],"trans-aortic":[55],"constriction":[56],"(TAC;":[57],"n=6)":[58],"compared":[60],"sham":[62,225],"operated":[63],"controls":[64],"(Sham;":[65],"n=5).":[66],"Compared":[67,190],"sham,":[69,192],"TAC":[70,105,168,198],"had":[72],"increased":[73,78,120,247],"LV":[74,79,95,146,244],"lung":[76],"weights,":[77],"end-diastolic":[80],"pressure":[81],"(4\u00b12":[82],"vs":[83,91,108,171,223,226],"25\u00b15":[84],"mmHg,":[85],"p<0.01)":[86],"reduced":[88,193,263],"dP/dT":[89],"(8249\u00b1596":[90],"4786\u00b1984":[92],"mmHg/sec,":[93],"p<0.01).":[94],"collagen":[96,114,133,160,188,250,264,270],"abundance":[97],"by":[98,121],"picrosirius":[99],"red":[100],"staining":[101],"higher":[103,158],"(1\u00b10.1":[107],"35\u00b112%,":[109],"p<0.05)":[110],"Type":[112,248],"I":[113,249],"mRNA":[115],"protein":[117,252],"levels":[118],"80-fold":[122],"2-fold,":[124],"respectively":[125],"(p<0.05":[126],"for":[127,284],"both).":[128],"Using":[129,149],"non-linear":[130],"optical":[131],"microscopy,":[132],"fiber":[134],"organization,":[135],"content":[136,161],"cross-linking":[138,186,194,265],"quantified":[140],"from":[141],"decellularized":[142,237],"sections":[143],"the":[145,232,258,275],"free":[147],"wall.":[148],"cumulative":[150],"second":[151],"harmonic":[152],"generation":[153],"(SHG),":[154],"trend":[156],"total":[159],"per":[162,187],"tissue":[163],"volume":[164],"observed":[166,196,219],"(0.02\u00b10.01":[170],"0.05\u00b10.3,":[172],"p=0.09).":[173],"Two":[174],"photon":[175],"excited":[176],"fluorescence":[177],"within":[178],"SHG":[179],"positive":[180],"voxels":[181],"used":[183],"measure":[185],"fiber.":[189],"(0.2":[200],"\u00b10.1":[201],"vs.":[202],"0.11\u00b10.04,":[203],"p=0.02).":[204],"Next,":[205],"using":[206],"tensile":[207],"testing":[209],"no":[210],"difference":[211],"average":[213],"stiffness":[215],"(elastic":[216],"modulus)":[217],"between":[220],"groups":[221],"(31\u00b115":[222],"24\u00b17,":[224],"TAC,":[227,257],"p=0.19).":[228],"Conclusions:":[229],"This":[230],"first":[233],"murine":[241],"Despite":[246],"mRNA,":[251],"histologic":[254],"fibrosis":[255],"after":[256],"showed":[262],"suggestive":[266],"functionally":[268],"immature":[269],"deposition.":[271],"Functional":[272],"maturation":[273],"may":[277],"be":[278],"an":[279],"important":[280],"target":[281],"therapy":[283]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2018-08-22T00:00:00"}