{"id":"https://openalex.org/W2886858643","doi":"https://doi.org/10.1080/20477724.2018.1480137","title":"Simplified MLST scheme for direct typing of Leptospira human clinical samples","display_name":"Simplified MLST scheme for direct typing of Leptospira human clinical samples","publication_year":2018,"publication_date":"2018-05-19","ids":{"openalex":"https://openalex.org/W2886858643","doi":"https://doi.org/10.1080/20477724.2018.1480137","mag":"2886858643","pmid":"https://pubmed.ncbi.nlm.nih.gov/30064347","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6147079"},"language":"en","primary_location":{"id":"doi:10.1080/20477724.2018.1480137","doi":"https://doi.org/10.1080/20477724.2018.1480137","is_oa":false,"landing_page_url":"https://doi.org/10.1080/20477724.2018.1480137","pdf_url":null,"source":{"id":"https://openalex.org/S2505153014","display_name":"Pathogens and Global Health","issn_l":"2047-7724","issn":["2047-7724","2047-7732"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens and Global Health","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6147079","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033342904","display_name":"Vanina Varni","orcid":"https://orcid.org/0009-0003-6021-916X","observed_orcids":["https://orcid.org/0009-0003-6021-916X"]},"institutions":[{"id":"https://openalex.org/I4210153948","display_name":"National Agricultural Technology Institute","ror":"https://ror.org/04wm52x94","country_code":"AR","type":"government","lineage":["https://openalex.org/I4210153948"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Vanina Varni","raw_affiliation_strings":["Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina","institution_ids":["https://openalex.org/I4210153948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064191038","display_name":"Yosena Chiani","orcid":"https://orcid.org/0000-0002-0406-4461","observed_orcids":["https://orcid.org/0000-0002-0406-4461"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yosena Chiani","raw_affiliation_strings":["National Institute of Respiratory Diseases, Instituto Nacional de Enfermedades Respiratorias, Santa F\u00e9, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Respiratory Diseases, Instituto Nacional de Enfermedades Respiratorias, Santa F\u00e9, Argentina","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030642157","display_name":"Ariel Nagel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210153948","display_name":"National Agricultural Technology Institute","ror":"https://ror.org/04wm52x94","country_code":"AR","type":"government","lineage":["https://openalex.org/I4210153948"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Ariel Nagel","raw_affiliation_strings":["Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina","institution_ids":["https://openalex.org/I4210153948"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011275750","display_name":"Paula Ruybal","orcid":"https://orcid.org/0000-0002-2365-7000","observed_orcids":["https://orcid.org/0000-0002-2365-7000"]},"institutions":[{"id":"https://openalex.org/I24354313","display_name":"Universidad de Buenos Aires","ror":"https://ror.org/0081fs513","country_code":"AR","type":"education","lineage":["https://openalex.org/I24354313"]}],"countries":["AR"],"is_corresponding":false,"raw_author_name":"Paula Ruybal","raw_affiliation_strings":["Institute of Research in Microbiology and Medical Parasitology (IMPAM), School of Medicine, University of Buenos Aires, Buenos Aires, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Research in Microbiology and Medical Parasitology (IMPAM), School of Medicine, University of Buenos Aires, Buenos Aires, Argentina","institution_ids":["https://openalex.org/I24354313"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004983754","display_name":"Norma B. Vanasco","orcid":"https://orcid.org/0000-0002-6134-1031","observed_orcids":["https://orcid.org/0000-0002-6134-1031"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Norma Bibiana Vanasco","raw_affiliation_strings":["National Institute of Respiratory Diseases, Instituto Nacional de Enfermedades Respiratorias, Santa F\u00e9, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Respiratory Diseases, Instituto Nacional de Enfermedades Respiratorias, Santa F\u00e9, Argentina","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5002986605","display_name":"Karina C. Caimi","orcid":"https://orcid.org/0000-0002-3788-9064","observed_orcids":["https://orcid.org/0000-0002-3788-9064"]},"institutions":[{"id":"https://openalex.org/I4210153948","display_name":"National Agricultural Technology Institute","ror":"https://ror.org/04wm52x94","country_code":"AR","type":"government","lineage":["https://openalex.org/I4210153948"]}],"countries":["AR"],"is_corresponding":true,"raw_author_name":"Karina Caimi","raw_affiliation_strings":["Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biotechnology Institute, National Institute of Agropecuarian Technology (INTA), Hurlingham, Buenos Aires, Argentina","institution_ids":["https://openalex.org/I4210153948"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5002986605"],"corresponding_institution_ids":["https://openalex.org/I4210153948"],"apc_list":null,"apc_paid":null,"fwci":0.9781,"has_fulltext":false,"cited_by_count":13,"citation_normalized_percentile":{"value":0.69503389,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"112","issue":"4","first_page":"203","last_page":"209"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12223","display_name":"Leptospirosis research and findings","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10823","display_name":"Research on Leishmaniasis Studies","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10486","display_name":"Salmonella and Campylobacter epidemiology","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/leptospira","display_name":"Leptospira","score":0.9890000224113464},{"id":"https://openalex.org/keywords/leptospirosis","display_name":"leptospirosis","score":0.9570000171661377},{"id":"https://openalex.org/keywords/multilocus-sequence-typing","display_name":"multilocus sequence typing","score":0.9929999709129333},{"id":"https://openalex.org/keywords/serogroups","display_name":"serogroups","score":0.6240000128746033},{"id":"https://openalex.org/keywords/allelic-profiles","display_name":"allelic profiles","score":0.8339999914169312},{"id":"https://openalex.org/keywords/strain-characterization","display_name":"strain characterization","score":0.9240000247955322},{"id":"https://openalex.org/keywords/disease-surveillance","display_name":"disease surveillance","score":0.6200000047683716},{"id":"https://openalex.org/keywords/zoonoses","display_name":"zoonoses","score":0.7440000176429749}],"concepts":[{"id":"https://openalex.org/C77434933","wikidata":"https://www.wikidata.org/wiki/Q4043440","display_name":"Multilocus sequence typing","level":4,"score":0.9154529571533203},{"id":"https://openalex.org/C2780542935","wikidata":"https://www.wikidata.org/wiki/Q133841","display_name":"Leptospira","level":3,"score":0.8599532842636108},{"id":"https://openalex.org/C2781209916","wikidata":"https://www.wikidata.org/wiki/Q3215756","display_name":"Typing","level":2,"score":0.8123276233673096},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.5937521457672119},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5736629962921143},{"id":"https://openalex.org/C2778033228","wikidata":"https://www.wikidata.org/wiki/Q155098","display_name":"Leptospirosis","level":2,"score":0.5417571067810059},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.4472092092037201},{"id":"https://openalex.org/C2776353676","wikidata":"https://www.wikidata.org/wiki/Q182672","display_name":"Zoonosis","level":2,"score":0.4433676600456238},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4206876754760742},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.40300828218460083},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.2869005799293518},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.16070160269737244},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09866580367088318}],"mesh":[{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007919","descriptor_name":"Leptospira","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D007922","descriptor_name":"Leptospirosis","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017720","descriptor_name":"Molecular Epidemiology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D058885","descriptor_name":"Multilocus Sequence Typing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1080/20477724.2018.1480137","doi":"https://doi.org/10.1080/20477724.2018.1480137","is_oa":false,"landing_page_url":"https://doi.org/10.1080/20477724.2018.1480137","pdf_url":null,"source":{"id":"https://openalex.org/S2505153014","display_name":"Pathogens and Global Health","issn_l":"2047-7724","issn":["2047-7724","2047-7732"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens and Global Health","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6147079","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6147079","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathog Glob Health","raw_type":"Text"},{"id":"pmid:30064347","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30064347","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathogens and global health","raw_type":"Evaluation Study"},{"id":"pmh:oai:ri.conicet.gov.ar:11336/165617","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/11336/165617","pdf_url":null,"source":{"id":"https://openalex.org/S4306402455","display_name":"CONICET Digital (CONICET)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210148385","host_organization_name":"Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society","host_organization_lineage":["https://openalex.org/I4210148385"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6147079","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6147079","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Pathog Glob Health","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1279688354","display_name":null,"funder_award_id":"PICT-2012","funder_id":"https://openalex.org/F4320334832","funder_display_name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"},{"id":"https://openalex.org/G7184247445","display_name":"Genotipos mayoritarios de Leptospira sp en Argentina. An\u00e1lisis transcript\u00f3mico de su proceso infectivo in vivo.","funder_award_id":"PICT-2012-0369","funder_id":"https://openalex.org/F4320334832","funder_display_name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica"}],"funders":[{"id":"https://openalex.org/F4320334832","display_name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","ror":"https://ror.org/03stxzb56"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":21,"referenced_works":["https://openalex.org/W1508716285","https://openalex.org/W1815051871","https://openalex.org/W1969042907","https://openalex.org/W1969905354","https://openalex.org/W1999113291","https://openalex.org/W2016523018","https://openalex.org/W2030988668","https://openalex.org/W2068504968","https://openalex.org/W2093129871","https://openalex.org/W2103546861","https://openalex.org/W2130121207","https://openalex.org/W2132632499","https://openalex.org/W2141559777","https://openalex.org/W2144728133","https://openalex.org/W2157801459","https://openalex.org/W2171251530","https://openalex.org/W2182844073","https://openalex.org/W2218406220","https://openalex.org/W2407029875","https://openalex.org/W2523690304","https://openalex.org/W2749693261"],"related_works":["https://openalex.org/W3207149835","https://openalex.org/W2523690304","https://openalex.org/W2182844073","https://openalex.org/W1815051871","https://openalex.org/W2412686429","https://openalex.org/W2030988668","https://openalex.org/W3205230745","https://openalex.org/W2141559777","https://openalex.org/W2171251530","https://openalex.org/W4312142628"],"abstract_inverted_index":{"Leptospirosis":[0],"is":[1],"a":[2,28,119],"globally":[3],"distributed":[4],"zoonosis.":[5],"Epidemiological":[6],"data":[7],"are":[8],"scarce":[9],"and":[10,69,111,115],"present":[11,117],"major":[12],"challenge":[13],"because":[14],"of":[15,49,62,72,78,80,100,132,145],"the":[16,47,73,76,81,88,97,103,126,143],"varied":[17],"clinical":[18,58,107,138],"presentations.":[19],"Multilocus":[20],"Sequence":[21],"Typing":[22],"has":[23],"already":[24],"proven":[25],"to":[26,51,87,96,129],"be":[27],"robust":[29],"molecular":[30],"typing":[31,54,105],"method":[32],"providing":[33],"accurate":[34],"results":[35,94],"for":[36,147],"strain":[37],"characterization.":[38],"We":[39],"have":[40],"adapted":[41],"our":[42],"MLST":[43],"scheme":[44,65,123],"by":[45],"reducing":[46],"set":[48],"loci":[50],"facilitate":[52],"Leptospira":[53,67],"directly":[55],"from":[56],"human":[57],"samples.":[59],"The":[60,121],"application":[61],"this":[63],"3-locus":[64],"provides":[66],"species":[68],"allelic":[70,113],"profiles":[71],"samples":[74,139],"retaining":[75],"power":[77],"discrimination":[79],"whole":[82],"scheme.":[83],"Moreover,":[84],"an":[85],"approach":[86],"serogroups":[89,116],"was":[90],"also":[91],"achieved.":[92],"Our":[93],"contribute":[95],"epidemiological":[98,148],"study":[99],"Leptospirosis,":[101],"since":[102],"direct":[104],"on":[106],"specimens":[108],"could":[109],"detect":[110],"update":[112],"variants":[114],"in":[118,137],"region.":[120],"simplified":[122],"allowed":[124],"at":[125],"same":[127],"time":[128],"take":[130],"advantage":[131],"limited":[133],"genetic":[134],"material":[135],"available":[136],"that":[140],"may":[141],"increase":[142],"sources":[144],"information":[146],"monitoring.":[149]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2018-08-22T00:00:00"}