{"id":"https://openalex.org/W2886782802","doi":"https://doi.org/10.1002/cbic.201800350","title":"Probing Backbone Hydrogen Bonds in Proteins by Amide\u2010to\u2010Ester Mutations","display_name":"Probing Backbone Hydrogen Bonds in Proteins by Amide\u2010to\u2010Ester Mutations","publication_year":2018,"publication_date":"2018-08-07","ids":{"openalex":"https://openalex.org/W2886782802","doi":"https://doi.org/10.1002/cbic.201800350","mag":"2886782802","pmid":"https://pubmed.ncbi.nlm.nih.gov/30073762"},"language":"en","primary_location":{"id":"doi:10.1002/cbic.201800350","doi":"https://doi.org/10.1002/cbic.201800350","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.201800350","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5045372915","display_name":"Vita Sereikait\u00e9","orcid":"https://orcid.org/0000-0001-7497-4965","observed_orcids":["https://orcid.org/0000-0001-7497-4965"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Vita Sereikait\u0117","raw_affiliation_strings":["Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Thomas M. T. Jensen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Thomas M. T. Jensen","raw_affiliation_strings":["Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062421025","display_name":"Christian R. O. Bartling","orcid":"https://orcid.org/0000-0002-0602-4738","observed_orcids":["https://orcid.org/0000-0002-0602-4738"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Christian R. O. Bartling","raw_affiliation_strings":["Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066198767","display_name":"Per Jemth","orcid":"https://orcid.org/0000-0003-1516-7228","observed_orcids":["https://orcid.org/0000-0003-1516-7228"]},"institutions":[{"id":"https://openalex.org/I123387679","display_name":"Uppsala University","ror":"https://ror.org/048a87296","country_code":"SE","type":"education","lineage":["https://openalex.org/I123387679"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Per Jemth","raw_affiliation_strings":["Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 75123 Uppsala, Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Biochemistry and Microbiology, Uppsala University, BMC, Box 582, 75123 Uppsala, Sweden","institution_ids":["https://openalex.org/I123387679"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045259945","display_name":"Stephan Alexander Pless","orcid":"https://orcid.org/0000-0001-6654-114X","observed_orcids":["https://orcid.org/0000-0001-6654-114X"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":false,"raw_author_name":"Stephan A. Pless","raw_affiliation_strings":["Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark","institution_ids":["https://openalex.org/I124055696"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5060992391","display_name":"Kristian Str\u00f8mgaard","orcid":"https://orcid.org/0000-0003-2206-4737","observed_orcids":["https://orcid.org/0000-0003-2206-4737"]},"institutions":[{"id":"https://openalex.org/I124055696","display_name":"University of Copenhagen","ror":"https://ror.org/035b05819","country_code":"DK","type":"education","lineage":["https://openalex.org/I124055696"]}],"countries":["DK"],"is_corresponding":true,"raw_author_name":"Kristian Str\u00f8mgaard","raw_affiliation_strings":["Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark"],"raw_orcid":"https://orcid.org/0000-0003-2206-4737","affiliations":[{"raw_affiliation_string":"Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Universitetsparken 2, 2200 Copenhagen, Denmark","institution_ids":["https://openalex.org/I124055696"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5060992391"],"corresponding_institution_ids":["https://openalex.org/I124055696"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":0.142,"has_fulltext":false,"cited_by_count":21,"citation_normalized_percentile":{"value":0.10460895,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"19","issue":"20","first_page":"2136","last_page":"2145"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9336000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.9336000084877014,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.04989999905228615,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/backbone-hydrogen-bonds","display_name":"backbone hydrogen bonds","score":0.906000018119812},{"id":"https://openalex.org/keywords/ester-substitution","display_name":"ester substitution","score":0.49000000953674316},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.7049999833106995},{"id":"https://openalex.org/keywords/protein-recognition","display_name":"protein recognition","score":0.7929999828338623},{"id":"https://openalex.org/keywords/protein-structure","display_name":"protein structure","score":0.6119999885559082},{"id":"https://openalex.org/keywords/protein-function","display_name":"protein function","score":0.6340000033378601},{"id":"https://openalex.org/keywords/protein-ligation","display_name":"protein ligation","score":0.9819999933242798}],"concepts":[{"id":"https://openalex.org/C2778439535","wikidata":"https://www.wikidata.org/wiki/Q188777","display_name":"Amide","level":2,"score":0.7527028322219849},{"id":"https://openalex.org/C112887158","wikidata":"https://www.wikidata.org/wiki/Q169324","display_name":"Hydrogen bond","level":3,"score":0.7488784790039062},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5819953083992004},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.45945388078689575},{"id":"https://openalex.org/C31684184","wikidata":"https://www.wikidata.org/wiki/Q33293","display_name":"Peptide bond","level":3,"score":0.44328221678733826},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.4012068212032318},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.27469250559806824},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.24346783757209778},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.2032308578491211},{"id":"https://openalex.org/C32909587","wikidata":"https://www.wikidata.org/wiki/Q11369","display_name":"Molecule","level":2,"score":0.14522114396095276}],"mesh":[{"descriptor_ui":"D000577","descriptor_name":"Amides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004952","descriptor_name":"Esters","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006860","descriptor_name":"Hydrogen Bonding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011506","descriptor_name":"Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D016296","descriptor_name":"Mutagenesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018389","descriptor_name":"Codon, Nonsense","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/cbic.201800350","doi":"https://doi.org/10.1002/cbic.201800350","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.201800350","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},{"id":"pmid:30073762","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30073762","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chembiochem : a European journal of chemical biology","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:openaire_cris_publications/dad735a6-d6c0-44e8-a831-c64319d17811","doi":null,"is_oa":false,"landing_page_url":"https://researchprofiles.ku.dk/da/publications/dad735a6-d6c0-44e8-a831-c64319d17811","pdf_url":null,"source":{"id":"https://openalex.org/S4306401983","display_name":"Research at the University of Copenhagen (University of Copenhagen)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I124055696","host_organization_name":"University of Copenhagen","host_organization_lineage":["https://openalex.org/I124055696"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Sereikait\u0117, V, Jensen, T M T, Bartling, C R O, Jemth, P, Pless, S A & Str\u00f8mgaard, K 2018, 'Probing Backbone Hydrogen Bonds in Proteins by Amide-to-Ester Mutations', ChemBioChem, vol. 19, no. 20, pp. 2136-2145. https://doi.org/10.1002/cbic.201800350","raw_type":"info:eu-repo/semantics/review"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4802889375","display_name":null,"funder_award_id":"R139\u20102012\u201012390","funder_id":"https://openalex.org/F4320321999","funder_display_name":"Lundbeckfonden"}],"funders":[{"id":"https://openalex.org/F4320321999","display_name":"Lundbeckfonden","ror":"https://ror.org/03hz8wd80"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":113,"referenced_works":["https://openalex.org/W114527085","https://openalex.org/W157808733","https://openalex.org/W165066620","https://openalex.org/W193624147","https://openalex.org/W1483942114","https://openalex.org/W1485099091","https://openalex.org/W1485287758","https://openalex.org/W1515586503","https://openalex.org/W1522477379","https://openalex.org/W1841858761","https://openalex.org/W1963842427","https://openalex.org/W1963995993","https://openalex.org/W1965903509","https://openalex.org/W1967415448","https://openalex.org/W1969303801","https://openalex.org/W1970108447","https://openalex.org/W1971780683","https://openalex.org/W1974548955","https://openalex.org/W1974925207","https://openalex.org/W1975744163","https://openalex.org/W1975832531","https://openalex.org/W1983082406","https://openalex.org/W1983863167","https://openalex.org/W1985854865","https://openalex.org/W1987544957","https://openalex.org/W1991958052","https://openalex.org/W1993854766","https://openalex.org/W2001429268","https://openalex.org/W2001579035","https://openalex.org/W2001608530","https://openalex.org/W2002139917","https://openalex.org/W2005181420","https://openalex.org/W2006786639","https://openalex.org/W2007747833","https://openalex.org/W2011196844","https://openalex.org/W2014235532","https://openalex.org/W2017776794","https://openalex.org/W2023223425","https://openalex.org/W2024223377","https://openalex.org/W2024351982","https://openalex.org/W2029300076","https://openalex.org/W2032121822","https://openalex.org/W2033041174","https://openalex.org/W2040863157","https://openalex.org/W2041459336","https://openalex.org/W2041755933","https://openalex.org/W2043172087","https://openalex.org/W2043227266","https://openalex.org/W2043259878","https://openalex.org/W2045529907","https://openalex.org/W2047815622","https://openalex.org/W2051145405","https://openalex.org/W2055483261","https://openalex.org/W2057962001","https://openalex.org/W2059187210","https://openalex.org/W2067013502","https://openalex.org/W2068858946","https://openalex.org/W2069506919","https://openalex.org/W2071938141","https://openalex.org/W2074570440","https://openalex.org/W2074662484","https://openalex.org/W2076845200","https://openalex.org/W2077410458","https://openalex.org/W2080616775","https://openalex.org/W2081446858","https://openalex.org/W2082460632","https://openalex.org/W2083433988","https://openalex.org/W2085095736","https://openalex.org/W2086824482","https://openalex.org/W2086895725","https://openalex.org/W2091485628","https://openalex.org/W2101298197","https://openalex.org/W2105199969","https://openalex.org/W2107167859","https://openalex.org/W2114589487","https://openalex.org/W2116179149","https://openalex.org/W2120597250","https://openalex.org/W2122294662","https://openalex.org/W2125059729","https://openalex.org/W2130190017","https://openalex.org/W2131061181","https://openalex.org/W2134699260","https://openalex.org/W2137250659","https://openalex.org/W2137990147","https://openalex.org/W2138070139","https://openalex.org/W2138854816","https://openalex.org/W2140992384","https://openalex.org/W2143070723","https://openalex.org/W2143185570","https://openalex.org/W2148405034","https://openalex.org/W2148946251","https://openalex.org/W2149793392","https://openalex.org/W2159215032","https://openalex.org/W2160480536","https://openalex.org/W2160583363","https://openalex.org/W2167064566","https://openalex.org/W2211918045","https://openalex.org/W2329336071","https://openalex.org/W2340625992","https://openalex.org/W2346810207","https://openalex.org/W2394679183","https://openalex.org/W2549897046","https://openalex.org/W2612541225","https://openalex.org/W2735227557","https://openalex.org/W2763278454","https://openalex.org/W2887987298","https://openalex.org/W2951064874","https://openalex.org/W4213262935","https://openalex.org/W4230408684","https://openalex.org/W4242435936","https://openalex.org/W4244195823","https://openalex.org/W4251658274","https://openalex.org/W4252028569"],"related_works":["https://openalex.org/W1485287758","https://openalex.org/W2036033596","https://openalex.org/W2082460632","https://openalex.org/W2041459336","https://openalex.org/W2068858946","https://openalex.org/W2051145405","https://openalex.org/W2027886505","https://openalex.org/W1965903509","https://openalex.org/W2070091907","https://openalex.org/W2040863157"],"abstract_inverted_index":{"All":[0],"proteins":[1],"contain":[2],"characteristic":[3],"backbones":[4],"formed":[5],"of":[6,19,48,81,92],"consecutive":[7],"amide":[8,57],"bonds,":[9],"which":[10],"can":[11,85],"engage":[12],"in":[13,96],"hydrogen":[14,73,94],"bonds.":[15,74],"However,":[16],"the":[17,49,55],"importance":[18],"these":[20],"is":[21,66],"not":[22],"easily":[23],"addressed":[24],"by":[25],"conventional":[26],"technologies":[27,36],"that":[28,62],"only":[29],"allow":[30,45],"for":[31,46],"side-chain":[32],"substitutions.":[33],"By":[34],"contrast,":[35],"such":[37],"as":[38],"nonsense":[39],"suppression":[40],"mutagenesis":[41],"and":[42,101],"protein":[43,50,97],"ligation":[44],"manipulation":[47],"backbone.":[51],"In":[52,75],"particular,":[53],"replacing":[54],"backbone":[56],"groups":[58],"with":[59],"ester":[60],"groups,":[61],"is,":[63],"amide-to-ester":[64,83],"mutations,":[65],"a":[67],"powerful":[68],"tool":[69],"to":[70,88],"examine":[71],"backbone-mediated":[72,93],"this":[76],"minireview,":[77],"we":[78],"showcase":[79],"examples":[80],"how":[82],"mutations":[84],"be":[86],"used":[87],"uncover":[89],"pivotal":[90],"roles":[91],"bonds":[95],"recognition,":[98],"folding,":[99],"function,":[100],"structure.":[102]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2018-08-22T00:00:00"}