{"id":"https://openalex.org/W2886524403","doi":"https://doi.org/10.3791/58061","title":"Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow","display_name":"Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow","publication_year":2018,"publication_date":"2018-07-29","ids":{"openalex":"https://openalex.org/W2886524403","doi":"https://doi.org/10.3791/58061","mag":"2886524403","pmid":"https://pubmed.ncbi.nlm.nih.gov/30102291","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6126592"},"language":"en","primary_location":{"id":"doi:10.3791/58061","doi":"https://doi.org/10.3791/58061","is_oa":false,"landing_page_url":"https://doi.org/10.3791/58061","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6126592","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042417204","display_name":"Alberto Y\u00e1\u00f1ez","orcid":"https://orcid.org/0000-0002-7719-1963","observed_orcids":["https://orcid.org/0000-0002-7719-1963"]},"institutions":[{"id":"https://openalex.org/I1282927834","display_name":"Cedars-Sinai Medical Center","ror":"https://ror.org/02pammg90","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1282927834"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Alberto Y\u00e1\u00f1ez","raw_affiliation_strings":["Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center; Research Division of Immunology, Cedars-Sinai Medical Center","Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center","Research Division of Immunology, Cedars-Sinai Medical Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center; Research Division of Immunology, Cedars-Sinai Medical Center","institution_ids":["https://openalex.org/I1282927834"]},{"raw_affiliation_string":"Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center","institution_ids":["https://openalex.org/I1282927834"]},{"raw_affiliation_string":"Research Division of Immunology, Cedars-Sinai Medical Center","institution_ids":["https://openalex.org/I1282927834"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042050280","display_name":"Helen S. Goodridge","orcid":"https://orcid.org/0000-0001-8097-2413","observed_orcids":["https://orcid.org/0000-0001-8097-2413"]},"institutions":[{"id":"https://openalex.org/I1282927834","display_name":"Cedars-Sinai Medical Center","ror":"https://ror.org/02pammg90","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1282927834"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Helen S. Goodridge","raw_affiliation_strings":["Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center; Research Division of Immunology, Cedars-Sinai Medical Center; helen.goodridge@csmc.edu","Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center","Research Division of Immunology, Cedars-Sinai Medical Center"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center; Research Division of Immunology, Cedars-Sinai Medical Center; helen.goodridge@csmc.edu","institution_ids":["https://openalex.org/I1282927834"]},{"raw_affiliation_string":"Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center","institution_ids":["https://openalex.org/I1282927834"]},{"raw_affiliation_string":"Research Division of Immunology, Cedars-Sinai Medical Center","institution_ids":["https://openalex.org/I1282927834"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5042050280"],"corresponding_institution_ids":["https://openalex.org/I1282927834"],"apc_list":null,"apc_paid":null,"fwci":0.1792,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.54042858,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":null,"issue":"137","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9223999977111816,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.9223999977111816,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11313","display_name":"Immune cells in cancer","score":0.022199999541044235,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12919","display_name":"Blood disorders and treatments","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/myeloid-progenitor-cells","display_name":"myeloid progenitor cells","score":0.9950000047683716},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.9700000286102295},{"id":"https://openalex.org/keywords/magnetic-activated-cell-sorting","display_name":"magnetic cell separation","score":0.890999972820282},{"id":"https://openalex.org/keywords/flow-cytometry-gating-strategy","display_name":"flow cytometry gating strategy","score":0.7609999775886536},{"id":"https://openalex.org/keywords/granulocytic-differentiation","display_name":"granulocytic differentiation","score":0.902999997138977},{"id":"https://openalex.org/keywords/monocyte-differentiation","display_name":"monocyte differentiation","score":0.9980000257492065},{"id":"https://openalex.org/keywords/dendritic-cell-differentiation","display_name":"dendritic cell differentiation","score":0.9890000224113464},{"id":"https://openalex.org/keywords/leukemic-transformation","display_name":"leukemic transformation","score":0.800000011920929},{"id":"https://openalex.org/keywords/mice","display_name":"mice","score":0.3880000114440918}],"concepts":[{"id":"https://openalex.org/C201750760","wikidata":"https://www.wikidata.org/wiki/Q1896218","display_name":"Progenitor cell","level":3,"score":0.8015280365943909},{"id":"https://openalex.org/C2779282312","wikidata":"https://www.wikidata.org/wiki/Q1956561","display_name":"Myeloid","level":2,"score":0.7579355835914612},{"id":"https://openalex.org/C2780007613","wikidata":"https://www.wikidata.org/wiki/Q546523","display_name":"Bone marrow","level":2,"score":0.7071173787117004},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6596734523773193},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.6339723467826843},{"id":"https://openalex.org/C109159458","wikidata":"https://www.wikidata.org/wiki/Q919283","display_name":"Haematopoiesis","level":3,"score":0.5358608365058899},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5294727087020874},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.5208274126052856},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.4894348680973053},{"id":"https://openalex.org/C15729860","wikidata":"https://www.wikidata.org/wiki/Q2329919","display_name":"Progenitor","level":4,"score":0.44328951835632324},{"id":"https://openalex.org/C2781184567","wikidata":"https://www.wikidata.org/wiki/Q107244","display_name":"Monocyte","level":2,"score":0.41166719794273376},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.36700239777565}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001853","descriptor_name":"Bone Marrow","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023461","descriptor_name":"Myeloid Progenitor Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.3791/58061","doi":"https://doi.org/10.3791/58061","is_oa":false,"landing_page_url":"https://doi.org/10.3791/58061","pdf_url":null,"source":{"id":"https://openalex.org/S199738284","display_name":"Journal of Visualized Experiments","issn_l":"1940-087X","issn":["1940-087X","2834-8400"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320416","host_organization_name":"MyJOVE","host_organization_lineage":["https://openalex.org/P4310320416"],"host_organization_lineage_names":["MyJOVE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6126592","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6126592","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},{"id":"pmid:30102291","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30102291","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of visualized experiments : JoVE","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6126592","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6126592","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Vis Exp","raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306571","display_name":"American Society of Hematology","ror":"https://ror.org/02nw48b86"},{"id":"https://openalex.org/F4320307136","display_name":"American Association of Immunologists","ror":"https://ror.org/04q9fhm49"},{"id":"https://openalex.org/F4320313996","display_name":"Cedars-Sinai Medical Center","ror":"https://ror.org/02pammg90"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W2048793063","https://openalex.org/W2050988558","https://openalex.org/W2054581136","https://openalex.org/W2090786449","https://openalex.org/W2094930641","https://openalex.org/W2110981938","https://openalex.org/W2142750845","https://openalex.org/W2342126943","https://openalex.org/W2411332746","https://openalex.org/W2509315794","https://openalex.org/W2515907404","https://openalex.org/W2769350174"],"related_works":["https://openalex.org/W2331065883","https://openalex.org/W4206447508","https://openalex.org/W2885529756","https://openalex.org/W4285219205","https://openalex.org/W137866042","https://openalex.org/W1495894459","https://openalex.org/W2903905952","https://openalex.org/W1522736440","https://openalex.org/W2473789245","https://openalex.org/W2027509917"],"abstract_inverted_index":{"Myeloid":[0],"progenitors":[1,131,154],"that":[2,90],"yield":[3],"neutrophils,":[4],"monocytes":[5],"and":[6,14,24,33,114,128,163,185,193,197,205,210],"dendritic":[7],"cells":[8],"(DCs)":[9],"can":[10,40],"be":[11],"identified":[12],"in":[13,69,191,194],"isolated":[15],"from":[16,132],"the":[17,31,49,73,94,115,121],"bone":[18,87,136,147],"marrow":[19,88,137,148],"of":[20,30,36,102,111,123,126,146,166,190,207],"mice":[21],"for":[22,61,93,152,187],"hematological":[23],"immunological":[25],"analyses.":[26],"For":[27],"example,":[28],"studies":[29,107],"cellular":[32],"molecular":[34],"properties":[35],"myeloid":[37,62,130,167],"progenitor":[38,63,168,183],"populations":[39],"reveal":[41],"mechanisms":[42,206],"underlying":[43],"leukemic":[44],"transformation,":[45],"or":[46],"demonstrate":[47],"how":[48],"immune":[50],"system":[51],"responds":[52],"to":[53],"pathogen":[54],"exposure.":[55],"Previously":[56],"described":[57,135],"flow":[58,171],"cytometry":[59,172],"strategies":[60,85],"identification":[64,165],"have":[65,108],"enabled":[66],"significant":[67],"advances":[68],"many":[70],"fields,":[71],"but":[72,97],"fractions":[74,89],"they":[75],"identify":[76],"are":[77,91],"very":[78],"heterogeneous.":[79],"The":[80,139],"most":[81],"commonly":[82],"used":[83],"gating":[84],"define":[86],"enriched":[92],"desired":[95],"populations,":[96],"also":[98],"contain":[99],"large":[100],"numbers":[101],"\"contaminating\"":[103],"progenitors.":[104],"Our":[105],"recent":[106],"resolved":[109],"much":[110],"this":[112],"heterogeneity,":[113],"protocol":[116,140],"we":[117],"present":[118],"here":[119],"permits":[120,182],"isolation":[122,145,186],"6":[124],"subpopulations":[125],"oligopotent":[127],"lineage-committed":[129],"2":[133],"previously":[134],"fractions.":[138],"describes":[141],"3":[142],"stages:":[143],"1)":[144],"cells,":[149],"2)":[150],"enrichment":[151],"hematopoietic":[153],"by":[155,161,170],"magnetic-activated":[156],"cell":[157,175],"sorting":[158],"(lineage":[159],"depletion":[160],"MACS),":[162],"3)":[164],"subsets":[169],"(including":[173],"fluorescence-activated":[174],"sorting,":[176],"FACS,":[177],"if":[178],"desired).":[179],"This":[180],"approach":[181],"quantification":[184],"a":[188],"variety":[189],"vitro":[192],"vivo":[195],"applications,":[196],"has":[198],"already":[199],"yielded":[200],"novel":[201],"insight":[202],"into":[203],"pathways":[204],"neutrophil,":[208],"monocyte,":[209],"DC":[211],"differentiation.":[212]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-08-22T00:00:00"}