{"id":"https://openalex.org/W2886444263","doi":"https://doi.org/10.1002/iub.1873","title":"Mass spectrometry\u2010based proteomic analysis reveals the interacting partners of lipin1","display_name":"Mass spectrometry\u2010based proteomic analysis reveals the interacting partners of lipin1","publication_year":2018,"publication_date":"2018-08-01","ids":{"openalex":"https://openalex.org/W2886444263","doi":"https://doi.org/10.1002/iub.1873","mag":"2886444263","pmid":"https://pubmed.ncbi.nlm.nih.gov/30092116"},"language":"en","primary_location":{"id":"doi:10.1002/iub.1873","doi":"https://doi.org/10.1002/iub.1873","is_oa":false,"landing_page_url":"https://doi.org/10.1002/iub.1873","pdf_url":null,"source":{"id":"https://openalex.org/S203723150","display_name":"IUBMB Life","issn_l":"1521-6543","issn":["1521-6543","1521-6551"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IUBMB Life","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Jian Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jian Wang","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101809188","display_name":"Song Wang","orcid":"https://orcid.org/0000-0002-4764-1417","observed_orcids":["https://orcid.org/0000-0002-4764-1417"]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Song Wang","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036761110","display_name":"Chongjia Yan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chongjia Yan","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032557475","display_name":"Yunxia Deng","orcid":"https://orcid.org/0000-0001-5861-3895","observed_orcids":["https://orcid.org/0000-0001-5861-3895"]},"institutions":[{"id":"https://openalex.org/I181326427","display_name":"Donghua University","ror":"https://ror.org/035psfh38","country_code":"CN","type":"education","lineage":["https://openalex.org/I181326427"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yunxia Deng","raw_affiliation_strings":["College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road, Shanghai, 201620 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road, Shanghai, 201620 China","institution_ids":["https://openalex.org/I181326427"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101484181","display_name":"Zhiwei Huang","orcid":"https://orcid.org/0000-0002-7140-5115","observed_orcids":["https://orcid.org/0000-0002-7140-5115"]},"institutions":[{"id":"https://openalex.org/I181326427","display_name":"Donghua University","ror":"https://ror.org/035psfh38","country_code":"CN","type":"education","lineage":["https://openalex.org/I181326427"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhiwei Huang","raw_affiliation_strings":["College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road, Shanghai, 201620 China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 Renmin Road, Shanghai, 201620 China","institution_ids":["https://openalex.org/I181326427"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059032368","display_name":"Ping Shi","orcid":"https://orcid.org/0000-0002-6904-2802","observed_orcids":["https://orcid.org/0000-0002-6904-2802"]},"institutions":[{"id":"https://openalex.org/I143593769","display_name":"East China University of Science and Technology","ror":"https://ror.org/01vyrm377","country_code":"CN","type":"education","lineage":["https://openalex.org/I143593769"]},{"id":"https://openalex.org/I4391768089","display_name":"State Key Laboratory of Bioreactor Engineering","ror":"https://ror.org/02hqczw19","country_code":"CN","type":"facility","lineage":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Ping Shi","raw_affiliation_strings":["State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China"],"raw_orcid":"https://orcid.org/0000-0002-6904-2802","affiliations":[{"raw_affiliation_string":"State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237 China","institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5059032368"],"corresponding_institution_ids":["https://openalex.org/I143593769","https://openalex.org/I4391768089"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":0.108,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.39789729,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"70","issue":"8","first_page":"753","last_page":"762"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.8446999788284302,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.8446999788284302,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.0820000022649765,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12465","display_name":"Cancer, Lipids, and Metabolism","score":0.032499998807907104,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lipin-1","display_name":"LPIN1","score":0.9980000257492065},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.9559999704360962},{"id":"https://openalex.org/keywords/phosphatidic-acid-phosphatase","display_name":"phosphatidate phosphohydrolase","score":0.8539999723434448},{"id":"https://openalex.org/keywords/lipid-metabolism","display_name":"lipid metabolism","score":0.6959999799728394},{"id":"https://openalex.org/keywords/eef1a1","display_name":"eEF1A1","score":0.9259999990463257},{"id":"https://openalex.org/keywords/co-transcriptional-regulation","display_name":"co-transcriptional regulation","score":0.8360000252723694},{"id":"https://openalex.org/keywords/protein-protein-interaction-network","display_name":"protein-protein interaction network","score":0.6420000195503235},{"id":"https://openalex.org/keywords/hepatocytes","display_name":"hepatocytes","score":0.6100000143051147},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.7720000147819519}],"concepts":[{"id":"https://openalex.org/C71829478","wikidata":"https://www.wikidata.org/wiki/Q901305","display_name":"Immunoprecipitation","level":3,"score":0.8385323286056519},{"id":"https://openalex.org/C174510640","wikidata":"https://www.wikidata.org/wiki/Q489618","display_name":"HEK 293 cells","level":3,"score":0.7766218185424805},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.6617183685302734},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5034648776054382},{"id":"https://openalex.org/C104397665","wikidata":"https://www.wikidata.org/wiki/Q860947","display_name":"Proteome","level":2,"score":0.4936593472957611},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.45892268419265747},{"id":"https://openalex.org/C46111723","wikidata":"https://www.wikidata.org/wiki/Q471857","display_name":"Proteomics","level":3,"score":0.420803427696228},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4041884243488312},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.39795851707458496},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31164735555648804},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18330147862434387},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.11205601692199707}],"mesh":[{"descriptor_ui":"D006657","descriptor_name":"Histones","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010711","descriptor_name":"Phosphatidate Phosphatase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013058","descriptor_name":"Mass Spectrometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020648","descriptor_name":"Peptide Elongation Factor 1","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D022781","descriptor_name":"Hepatocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D040901","descriptor_name":"Proteomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D047468","descriptor_name":"Immunoprecipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D050356","descriptor_name":"Lipid Metabolism","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060066","descriptor_name":"Protein Interaction Maps","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/iub.1873","doi":"https://doi.org/10.1002/iub.1873","is_oa":false,"landing_page_url":"https://doi.org/10.1002/iub.1873","pdf_url":null,"source":{"id":"https://openalex.org/S203723150","display_name":"IUBMB Life","issn_l":"1521-6543","issn":["1521-6543","1521-6551"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IUBMB Life","raw_type":"journal-article"},{"id":"pmid:30092116","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30092116","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IUBMB life","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G7317135814","display_name":"\u55b9\u5549\u884d\u751f\u7269\u62ee\u6297VB1\u8bf1\u5bfcTD\u6027\u72b6\u7684\u5206\u5b50\u673a\u5236\u7814\u7a76","funder_award_id":"31671309","funder_id":"https://openalex.org/F4320321001","funder_display_name":"National Natural Science Foundation of China"}],"funders":[{"id":"https://openalex.org/F4320321001","display_name":"National Natural Science Foundation of China","ror":"https://ror.org/01h0zpd94"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":47,"referenced_works":["https://openalex.org/W1501141911","https://openalex.org/W1523366628","https://openalex.org/W1525867888","https://openalex.org/W1560901632","https://openalex.org/W1584487774","https://openalex.org/W1964480889","https://openalex.org/W1990784348","https://openalex.org/W1992579613","https://openalex.org/W1998384937","https://openalex.org/W2000371661","https://openalex.org/W2002999352","https://openalex.org/W2006417571","https://openalex.org/W2011874398","https://openalex.org/W2014719123","https://openalex.org/W2015510902","https://openalex.org/W2024544617","https://openalex.org/W2026046421","https://openalex.org/W2029636772","https://openalex.org/W2037199116","https://openalex.org/W2063001525","https://openalex.org/W2065394512","https://openalex.org/W2076710572","https://openalex.org/W2081731386","https://openalex.org/W2084619201","https://openalex.org/W2088621072","https://openalex.org/W2089441009","https://openalex.org/W2098999612","https://openalex.org/W2103214205","https://openalex.org/W2108321390","https://openalex.org/W2116117181","https://openalex.org/W2126214612","https://openalex.org/W2128036243","https://openalex.org/W2132468746","https://openalex.org/W2140431789","https://openalex.org/W2144899103","https://openalex.org/W2149060516","https://openalex.org/W2149186189","https://openalex.org/W2149187763","https://openalex.org/W2160662703","https://openalex.org/W2164136158","https://openalex.org/W2170329898","https://openalex.org/W2183562820","https://openalex.org/W2184997735","https://openalex.org/W2273117071","https://openalex.org/W2289141937","https://openalex.org/W2321862995","https://openalex.org/W2625766497"],"related_works":["https://openalex.org/W2995384181","https://openalex.org/W7200882895","https://openalex.org/W1995682421","https://openalex.org/W7159765325","https://openalex.org/W2038438556","https://openalex.org/W2265745671","https://openalex.org/W2806593976","https://openalex.org/W2499835880","https://openalex.org/W4386529738","https://openalex.org/W4298088972"],"abstract_inverted_index":{"Abstract":[0],"As":[1],"a":[2,12,17],"lipin":[3],"family":[4],"founding":[5],"member,":[6],"lipin1":[7,53,70,81,113,126,155],"exerts":[8],"dual":[9],"functions":[10],"as":[11],"phosphatidate":[13],"phosphatase":[14],"enzyme":[15],"and/or":[16],"co\u2010transcriptional":[18],"regulator":[19],"in":[20,29,54,119,145,156,159],"lipid":[21],"metabolism.":[22],"In":[23,34],"fact,":[24],"it":[25],"is":[26],"also":[27,138],"involved":[28],"many":[30],"other":[31],"cell":[32],"processes.":[33,162],"this":[35],"study,":[36],"we":[37],"utilized":[38],"pull":[39],"down":[40],"assay":[41,61],"coupled":[42],"with":[43,76,123,140],"mass":[44,88],"spectrometry":[45],"(MS)":[46],"to":[47,68],"unravel":[48],"protein\u2013protein":[49],"interaction":[50,111],"networks":[51],"of":[52,92,101],"293T":[55,73,120],"human":[56],"embryonic":[57],"kidney":[58],"cells.":[59],"Pull\u2010down":[60],"on":[62,85],"the":[63,102],"Ni2+\u2010chelating":[64],"column":[65],"was":[66,116,143],"used":[67],"isolate":[69],"complexes":[71,82],"from":[72,97],"cells":[74,121,130,147],"transfected":[75,122],"6\u2010His":[77,124],"tagged":[78,125],"lipin1.":[79],"The":[80,109],"were":[83,95],"analyzed":[84],"Q":[86],"Exactive":[87],"spectrometer.":[89],"A":[90],"total":[91],"30":[93],"proteins":[94],"identified":[96],"label":[98],"free":[99],"quantitation":[100],"MS":[103],"data":[104],"by":[105,131,148],"Proteome":[106],"Discoverer":[107],"platform.":[108],"physical":[110],"between":[112],"and":[114,127,134],"eEF1A1":[115],"further":[117],"affirmed":[118],"hepatocyte":[128],"SMMC7721":[129,146],"protein":[132,149],"immunoprecipitation":[133],"immunofluorescence":[135],"microscopy.":[136],"Lipin1":[137],"interacted":[139],"HIST1H2BK,":[141],"which":[142],"confirmed":[144],"immunoprecipitation.":[150],"Our":[151],"proteomic":[152],"analysis":[153],"implicated":[154],"novel":[157],"roles":[158],"various":[160],"cellular":[161],"\u00a9":[163],"2018":[164,168],"IUBMB":[165],"Life,":[166],"70(8):753\u2013762,":[167]},"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-08-22T00:00:00"}