{"id":"https://openalex.org/W28859424","doi":null,"title":"Molecular and biochemical analyses of iodopsin in rd chick retina.","display_name":"Molecular and biochemical analyses of iodopsin in rd chick retina.","publication_year":1994,"publication_date":"1994-04-01","ids":{"openalex":"https://openalex.org/W28859424","mag":"28859424","pmid":"https://pubmed.ncbi.nlm.nih.gov/7512946"},"language":"en","primary_location":{"id":"pmid:7512946","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7512946","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082544318","display_name":"Susan L. Semple\u2010Rowland","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800717037","display_name":"University of Florida Health","ror":"https://ror.org/04tk2gy88","country_code":"US","type":"education","lineage":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]},{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S L Semple-Rowland","raw_affiliation_strings":["Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244","institution_ids":["https://openalex.org/I2800717037","https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034884540","display_name":"DANIEL A. GREEN","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"D A Green","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.4077,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.56316737,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"35","issue":"5","first_page":"2550","last_page":"7"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12236","display_name":"Photoreceptor and optogenetics research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10170","display_name":"Retinal Diseases and Treatments","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/iodopsin","display_name":"iodopsin","score":0.9959999918937683},{"id":"https://openalex.org/keywords/retinal-degeneration","display_name":"retinal degeneration","score":0.8949999809265137},{"id":"https://openalex.org/keywords/cone-visual-pigments","display_name":"cone visual pigments","score":0.8399999737739563},{"id":"https://openalex.org/keywords/inherited-retinal-degeneration","display_name":"inherited retinal degeneration","score":0.8420000076293945},{"id":"https://openalex.org/keywords/cdna-sequence","display_name":"cDNA sequence","score":0.574999988079071},{"id":"https://openalex.org/keywords/gene-transcription","display_name":"gene transcription","score":0.753000020980835},{"id":"https://openalex.org/keywords/post-translational-processing","display_name":"post-translational processing","score":0.9710000157356262},{"id":"https://openalex.org/keywords/chicken-retina","display_name":"chicken retina","score":0.550000011920929}],"concepts":[{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.880505383014679},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.7986834049224854},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7598724365234375},{"id":"https://openalex.org/C189482833","wikidata":"https://www.wikidata.org/wiki/Q869146","display_name":"cDNA library","level":4,"score":0.6474384665489197},{"id":"https://openalex.org/C177707886","wikidata":"https://www.wikidata.org/wiki/Q48520","display_name":"Northern blot","level":4,"score":0.5876567959785461},{"id":"https://openalex.org/C85265743","wikidata":"https://www.wikidata.org/wiki/Q885458","display_name":"Blot","level":3,"score":0.5808069705963135},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5435121655464172},{"id":"https://openalex.org/C2780827179","wikidata":"https://www.wikidata.org/wiki/Q422001","display_name":"Retinal","level":2,"score":0.4747393727302551},{"id":"https://openalex.org/C81439078","wikidata":"https://www.wikidata.org/wiki/Q2304142","display_name":"In situ hybridization","level":4,"score":0.4326850473880768},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.42378681898117065},{"id":"https://openalex.org/C133307689","wikidata":"https://www.wikidata.org/wiki/Q48749","display_name":"Southern blot","level":3,"score":0.41796964406967163},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3182866871356964},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1577615737915039},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1400023102760315}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009693","descriptor_name":"Nucleic Acid Hybridization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012160","descriptor_name":"Retina","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D012162","descriptor_name":"Retinal Degeneration","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012162","descriptor_name":"Retinal Degeneration","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012168","descriptor_name":"Retinal Pigments","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012168","descriptor_name":"Retinal Pigments","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015152","descriptor_name":"Blotting, Northern","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015153","descriptor_name":"Blotting, Western","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015342","descriptor_name":"DNA Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017299","descriptor_name":"Rod Opsins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"pmid:7512946","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7512946","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Investigative ophthalmology & visual science","raw_type":"Journal Article"},{"id":"mag:28859424","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7512946","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8895164824","display_name":"MODEL OF HEREDITARY BLINDNESS--PROTEIN ANALYSES","funder_award_id":"R01EY008340","funder_id":"https://openalex.org/F4320337350","funder_display_name":"National Eye Institute"}],"funders":[{"id":"https://openalex.org/F4320337350","display_name":"National Eye Institute","ror":"https://ror.org/03wkg3b53"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":45,"referenced_works":["https://openalex.org/W31827621","https://openalex.org/W103335656","https://openalex.org/W200675400","https://openalex.org/W1772681111","https://openalex.org/W1892420087","https://openalex.org/W1970286448","https://openalex.org/W1987248659","https://openalex.org/W1989837406","https://openalex.org/W2002875034","https://openalex.org/W2006760921","https://openalex.org/W2016537683","https://openalex.org/W2025692747","https://openalex.org/W2028803712","https://openalex.org/W2033578411","https://openalex.org/W2035629144","https://openalex.org/W2041102000","https://openalex.org/W2045292852","https://openalex.org/W2048408401","https://openalex.org/W2054622667","https://openalex.org/W2056023749","https://openalex.org/W2064304960","https://openalex.org/W2064851008","https://openalex.org/W2068155640","https://openalex.org/W2073469631","https://openalex.org/W2078123421","https://openalex.org/W2084259243","https://openalex.org/W2089037068","https://openalex.org/W2100837269","https://openalex.org/W2107177848","https://openalex.org/W2110356839","https://openalex.org/W2113626142","https://openalex.org/W2116669982","https://openalex.org/W2125105889","https://openalex.org/W2126800506","https://openalex.org/W2127259632","https://openalex.org/W2133304293","https://openalex.org/W2136727656","https://openalex.org/W2141435397","https://openalex.org/W2170412674","https://openalex.org/W2234885627","https://openalex.org/W2270778178","https://openalex.org/W2576734941","https://openalex.org/W2742977456","https://openalex.org/W2900778024","https://openalex.org/W4294185218"],"related_works":["https://openalex.org/W2012244059","https://openalex.org/W2006760921","https://openalex.org/W28536690","https://openalex.org/W2046571685","https://openalex.org/W4231007405","https://openalex.org/W2056023749","https://openalex.org/W1222827037","https://openalex.org/W2052494812","https://openalex.org/W1128584736","https://openalex.org/W2086230896"],"abstract_inverted_index":{"PURPOSE:":[0],"The":[1,29,262],"results":[2,234,263],"of":[3,9,11,31,43,52,112,128,136,140,176,188,209,235,253,264,269,276],"previous":[4],"immunocytochemical":[5],"and":[6,41,60,83,116,163,191,227,239,251],"electrophysiological":[7],"studies":[8],"retinas":[10],"rd":[12,260],"(retinal":[13],"degeneration)":[14],"chicks":[15],"suggest":[16,272],"that":[17,101,164,222,246,273],"the":[18,38,113,126,137,153,173,177,197,210,215,225,233,236,243,247,259,265],"iodopsin":[19,72,122,161,168,182,250,271,277],"cone":[20],"visual":[21],"pigment":[22],"may":[23,278],"be":[24,279],"defective":[25],"in":[26,48,134,172,201,214,224,258,281],"this":[27,32,44,49,254,282],"mutant.":[28,261,283],"goal":[30],"study":[33,66],"was":[34,218],"to":[35,65,105],"determine":[36],"if":[37],"primary":[39],"structure":[40],"synthesis":[42],"protein":[45],"is":[46],"normal":[47,159,257],"animal":[50],"model":[51],"inherited":[53],"retinal":[54,80,193],"degeneration.":[55],"METHODS:":[56],"Northern":[57],"cDNA":[58,79,123,162,237],"sequence":[59,238],"western":[61,186,266],"analyses":[62,268],"were":[63,73,90,142,150,183],"used":[64],"rd/rd":[67,71,78,117,167,192,216,270],"iodopsin.":[68,109],"cDNAs":[69],"encoding":[70,249],"obtained":[74],"by":[75,84],"screening":[76],"an":[77,121],"expression":[81],"library":[82],"reverse":[85],"transcription":[86,252],"PCR.":[87],"Western":[88],"blots":[89,187],"probed":[91],"with":[92,120,144,204],"either":[93],"R4":[94,195],"or":[95],"COS-1,":[96],"two":[97],"different":[98],"monoclonal":[99],"antibodies":[100],"have":[102],"been":[103],"shown":[104],"specifically":[106],"recognize":[107],"chicken":[108,160],"RESULTS:":[110],"Hybridization":[111],"+/+,":[114,189],"+/rd,":[115,190],"poly(A)+":[118],"RNA":[119],"probe":[124],"revealed":[125],"presence":[127],"a":[129],"single":[130],"1.5":[131],"kb":[132],"band":[133,180,200,213],"each":[135,202],"samples,":[138],"all":[139],"which":[141],"labeled":[143],"equal":[145,205],"intensity.":[146,206],"No":[147],"significant":[148],"differences":[149,171],"found":[151],"between":[152],"published":[154],"nucleic":[155],"acid":[156],"sequences":[157],"for":[158,166],"determined":[165],"cDNA.":[169],"Antibody-dependent":[170],"staining":[174],"intensity":[175],"34":[178,198,211],"kDa":[179,199,212],"containing":[181],"observed":[184,223],"on":[185,232],"protein.":[194],"stained":[196],"sample":[203,217],"COS-1":[207],"labeling":[208],"less":[219],"intense":[220],"than":[221],"+/+":[226],"+/rd":[228],"samples.":[229],"CONCLUSIONS:":[230],"Based":[231],"northern":[240],"blot":[241,267],"experiments,":[242],"authors":[244],"conclude":[245],"gene":[248,255],"are":[256],"post-translational":[274],"processing":[275],"abnormal":[280]},"counts_by_year":[{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}