{"id":"https://openalex.org/W2883126546","doi":null,"title":"Engineering of Acyl-CoA Metabolism for Production of Branched Chain Fatty Acids and Pikromycin in Streptomyces","display_name":"Engineering of Acyl-CoA Metabolism for Production of Branched Chain Fatty Acids and Pikromycin in Streptomyces","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2883126546","mag":"2883126546"},"language":"en","primary_location":{"id":"pmh:oai:s-space.snu.ac.kr:10371/140749","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10371/140749","pdf_url":null,"source":{"id":"https://openalex.org/S4306401345","display_name":"Seoul National University Open Repository (Seoul National University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139264467","host_organization_name":"Seoul National University","host_organization_lineage":["https://openalex.org/I139264467"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5009036959","display_name":"\uc774\uc815\uc0c1","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"\uc774\uc815\uc0c1","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5009036959"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.8895999789237976,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.8895999789237976,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.10189999639987946,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/branched-chain-fatty-acids","display_name":"branched-chain fatty acids","score":0.9380000233650208},{"id":"https://openalex.org/keywords/pikromycin","display_name":"pikromycin","score":0.9779999852180481},{"id":"https://openalex.org/keywords/streptomyces-coelicolor","display_name":"Streptomyces coelicolor","score":0.9169999957084656},{"id":"https://openalex.org/keywords/streptomyces-venezuelae","display_name":"Streptomyces venezuelae","score":1.0},{"id":"https://openalex.org/keywords/branched-chain-amino-acid-catabolism","display_name":"branched-chain amino acid catabolism","score":0.9629999995231628},{"id":"https://openalex.org/keywords/branched-chain-alpha-keto-acid-dehydrogenase","display_name":"branched-chain ketoacid dehydrogenase","score":0.8820000290870667},{"id":"https://openalex.org/keywords/acyl-coa-dehydrogenase","display_name":"acyl-CoA dehydrogenase","score":0.8640000224113464},{"id":"https://openalex.org/keywords/methylmalonyl-coa","display_name":"methylmalonyl-CoA","score":0.8629999756813049},{"id":"https://openalex.org/keywords/methylmalonyl-coa-mutase","display_name":"methylmalonyl-CoA mutase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/fatty-acid-beta-oxidation","display_name":"fatty acid beta-oxidation","score":0.718999981880188},{"id":"https://openalex.org/keywords/polyketide-synthase-gene-clusters","display_name":"polyketide synthase gene clusters","score":0.7710000276565552},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.5849999785423279},{"id":"https://openalex.org/keywords/polyketide-antibiotics","display_name":"polyketide antibiotics","score":0.6050000190734863}],"concepts":[{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.5843967199325562},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5323472619056702},{"id":"https://openalex.org/C2779920670","wikidata":"https://www.wikidata.org/wiki/Q1144013","display_name":"Streptomyces","level":3,"score":0.4269421398639679},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.41066673398017883},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3131706714630127},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11221253871917725},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.11206242442131042}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:s-space.snu.ac.kr:10371/140749","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10371/140749","pdf_url":null,"source":{"id":"https://openalex.org/S4306401345","display_name":"Seoul National University Open Repository (Seoul National University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139264467","host_organization_name":"Seoul National University","host_organization_lineage":["https://openalex.org/I139264467"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2883126546","doi":null,"is_oa":false,"landing_page_url":"https://s-space.snu.ac.kr/bitstream/10371/140749/1/000000149682.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W1599984390","https://openalex.org/W1681807331","https://openalex.org/W1938494640","https://openalex.org/W1975415070","https://openalex.org/W1976924054","https://openalex.org/W1991985821","https://openalex.org/W1994656768","https://openalex.org/W2009156161","https://openalex.org/W2019715038","https://openalex.org/W2025263880","https://openalex.org/W2029262835","https://openalex.org/W2034480830","https://openalex.org/W2035181180","https://openalex.org/W2041824793","https://openalex.org/W2052461535","https://openalex.org/W2057804106","https://openalex.org/W2065554136","https://openalex.org/W2079890831","https://openalex.org/W2084447564","https://openalex.org/W2104982324","https://openalex.org/W2106233702","https://openalex.org/W2111007558","https://openalex.org/W2131446858","https://openalex.org/W2142314320","https://openalex.org/W2143748029","https://openalex.org/W2161155802","https://openalex.org/W2319487633"],"related_works":["https://openalex.org/W7098514042","https://openalex.org/W2792308146","https://openalex.org/W3087873241","https://openalex.org/W7099822031","https://openalex.org/W2474456089","https://openalex.org/W2986486499","https://openalex.org/W3045679009","https://openalex.org/W2474750633","https://openalex.org/W4405906278","https://openalex.org/W7039492378"],"abstract_inverted_index":{"In":[0],"this":[1,222],"thesis,":[2],"branched":[3,19,50],"chain":[4,20,51],"fatty":[5,122,239],"acids":[6,22],"(BCFA)":[7],"and":[8,27,36,56,103,110,153,198,238],"polyketide":[9,65,129,236],"antibiotics,":[10],"pikromycin":[11],"(PKM),":[12],"were":[13,25,46,105,155,200],"overproduced":[14],"in":[15,33,38,42,64,96,114,257],"Streptomyces":[16,34,39,259],"species.":[17],"Primarily,":[18],"amino":[21],"(BCAA)":[23],"catabolism":[24],"examined":[26],"engineered":[28],"for":[29,249],"synthesis":[30,130,217],"of":[31,44,74,76,84,88,212,218,227,235,251,255],"BCFA":[32],"coelicolor,":[35,116],"PKM":[37,89,146,168],"venezuelae.":[40],"\\nIncreases":[41],"productions":[43,87,169,183,250],"BCFAs":[45],"achieved":[47],"by":[48,230],"overexpressing":[49],"\u03b1-keto":[52],"acid":[53,123],"dehydrogenase":[54,101],"(BCDH)":[55],"3-ketoacyl":[57],"acyl":[58],"carrier":[59],"protein":[60],"synthase":[61,66],"III":[62],"(KASIII)":[63],"gene":[67],"clusters":[68],"deleted":[69],"S.":[70,91,115],"coelicolor":[71],"strain.":[72],"Deletion":[73],"fadD":[75],"\u03b2-oxidation":[77],"was":[78,118,141,165,188,205],"also":[79],"performed":[80],"to":[81,120,127,157,167,232,246],"block":[82],"degradation":[83],"BCFAs.For":[85],"enhancing":[86],"from":[90,138,170,184,193],"venezuelae,":[92],"three":[93],"key":[94],"enzymes":[95],"BCAA":[97,194,228],"catabolism,":[98],"KASIII,":[99],"acyl-CoA":[100],"(ACAD),":[102],"BCDH":[104,152,197],"manipulated.":[106],"First,":[107],"using":[108],"promoter":[109],"5UTR":[111],"sets":[112],"developed":[113],"KASIII":[117,139],"repressed":[119],"lower":[121],"(FA)":[124],"synthesis,":[125],"but":[126,210],"increase":[128,158],"on":[131],"the":[132,145,159],"other":[133],"hand.":[134],"Unfortunately,":[135],"growth":[136],"defects":[137],"repression":[140],"too":[142],"severe":[143],"that":[144,181,192],"production":[147],"decreased.":[148],"As":[149],"a":[150,208],"result,":[151],"ACAD":[154],"overexpressed":[156,201],"fluxes":[160],"towards":[161],"methylmalonyl-CoA":[162,182],"productions.":[163],"It":[164,179,241],"compared":[166],"central":[171,185],"carbon":[172,186],"metabolism":[173,187,229],"with":[174],"Methylmalonyl-CoA":[175],"mutase":[176],"(MCM)":[177],"overexpression.":[178],"appeared":[180],"more":[189],"effective":[190],"than":[191],"catabolism.":[195],"When":[196],"MCM":[199],"together,":[202],"precursor":[203],"supplementation":[204],"no":[206],"longer":[207],"bottleneck,":[209],"activities":[211],"pikC":[213],"P450":[214],"monooxygenase":[215],"limited":[216],"pikromycin.":[219],"\\nIn":[220],"short,":[221],"study":[223],"explored":[224],"less-assessed":[225],"area":[226],"engineering,":[231],"generate":[233],"precursors":[234],"antibiotics":[237],"acids.":[240],"would":[242],"provide":[243],"general":[244],"insights":[245],"design":[247],"methodologies":[248],"various":[252,258],"metabolites":[253],"composed":[254],"acyl-CoAs":[256],"sp..":[260]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2018-08-03T00:00:00"}