{"id":"https://openalex.org/W28192206","doi":null,"title":"Group II intron thermophilic reverse transcriptases","display_name":"Group II intron thermophilic reverse transcriptases","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W28192206","mag":"28192206"},"language":"en","primary_location":{"id":"pmh:oai:opus.bath.ac.uk:27218","doi":null,"is_oa":false,"landing_page_url":"http://opus.bath.ac.uk/27218/1/Univ_Bath_PhD_2011_N_Voina.pdf","pdf_url":"http://opus.bath.ac.uk/27218/1/Univ_Bath_PhD_2011_N_Voina.pdf","source":{"id":"https://openalex.org/S4306401631","display_name":"The University of Bath Online Publications Store (The University of Bath)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I51601045","host_organization_name":"University of Bath","host_organization_lineage":["https://openalex.org/I51601045"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021478405","display_name":"Natasha J. Voina","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Natasha J. Voina","raw_affiliation_strings":["Department of Biology & Biochemistry"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biology & Biochemistry","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5021478405"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.7773000001907349,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.7773000001907349,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.0723000019788742,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0406000018119812,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/reverse-transcriptase","display_name":"reverse transcriptase","score":0.8199999928474426},{"id":"https://openalex.org/keywords/thermophilic-bacteria","display_name":"thermophilic bacteria","score":0.7749999761581421},{"id":"https://openalex.org/keywords/enzyme-engineering","display_name":"enzyme engineering","score":0.5789999961853027},{"id":"https://openalex.org/keywords/rna-secondary-structure","display_name":"RNA secondary structure","score":0.6949999928474426},{"id":"https://openalex.org/keywords/cdna-synthesis","display_name":"cDNA synthesis","score":0.7129999995231628},{"id":"https://openalex.org/keywords/enzyme-processivity","display_name":"enzyme processivity","score":0.6129999756813049}],"concepts":[{"id":"https://openalex.org/C156719811","wikidata":"https://www.wikidata.org/wiki/Q28774","display_name":"Reverse transcriptase","level":4,"score":0.7502102851867676},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.5982028245925903},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5264138579368591},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.4881756901741028},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.48577097058296204},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.4752759039402008},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4694359302520752},{"id":"https://openalex.org/C2756471","wikidata":"https://www.wikidata.org/wiki/Q206286","display_name":"DNA polymerase","level":3,"score":0.46387043595314026},{"id":"https://openalex.org/C27911776","wikidata":"https://www.wikidata.org/wiki/Q834023","display_name":"Thermophile","level":3,"score":0.4545920491218567},{"id":"https://openalex.org/C123894998","wikidata":"https://www.wikidata.org/wiki/Q425158","display_name":"Fusion protein","level":4,"score":0.44805407524108887},{"id":"https://openalex.org/C2780048736","wikidata":"https://www.wikidata.org/wiki/Q418713","display_name":"T7 RNA polymerase","level":5,"score":0.4272244870662689},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4223659634590149},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.4117940068244934},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.40413007140159607},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3923332095146179},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.38969284296035767},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.28230345249176025},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.16773444414138794},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.15529611706733704},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.09000372886657715},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:opus.bath.ac.uk:27218","doi":null,"is_oa":false,"landing_page_url":"http://opus.bath.ac.uk/27218/1/Univ_Bath_PhD_2011_N_Voina.pdf","pdf_url":"http://opus.bath.ac.uk/27218/1/Univ_Bath_PhD_2011_N_Voina.pdf","source":{"id":"https://openalex.org/S4306401631","display_name":"The University of Bath Online Publications Store (The University of Bath)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I51601045","host_organization_name":"University of Bath","host_organization_lineage":["https://openalex.org/I51601045"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:hdl:10068/983770","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/983770","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"mag:28192206","doi":null,"is_oa":false,"landing_page_url":"https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.538289","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1584506915","https://openalex.org/W1880181670","https://openalex.org/W1999455027","https://openalex.org/W2004773817","https://openalex.org/W2008518693","https://openalex.org/W2014976263","https://openalex.org/W2046165401","https://openalex.org/W2050717506","https://openalex.org/W2054036000","https://openalex.org/W2083648157","https://openalex.org/W2126436625","https://openalex.org/W2142581892","https://openalex.org/W2157890550","https://openalex.org/W2187158086","https://openalex.org/W2334397963"],"related_works":["https://openalex.org/W2088956868","https://openalex.org/W2167676559","https://openalex.org/W4390402945","https://openalex.org/W2135034945","https://openalex.org/W2111034160","https://openalex.org/W2769865067","https://openalex.org/W2767788891","https://openalex.org/W2013712884","https://openalex.org/W2147345802","https://openalex.org/W7172402958"],"abstract_inverted_index":{"A":[0],"reverse":[1,20],"transcription":[2],"reaction":[3,311],"allows":[4],"the":[5,40,43,59,78,90,110,187,218,221,242,263,271,274,292],"production":[6],"of":[7,42,85,92,109,131,151,189,238,245,273,279,285],"complementary":[8],"DNA":[9,64,226],"(cDNA)":[10],"using":[11,62,322],"an":[12,159,167,246,288,317],"RNA":[13,46,324],"template":[14],"and":[15,32,88,113,140,316],"relies":[16],"on":[17,116,212,269],"polymerases":[18,65],"displaying":[19],"transcriptase":[21],"(RT)":[22],"activity.":[23,228],"This":[24,75],"process,":[25],"with":[26,66,101,203],"major":[27,300],"applications":[28],"in":[29,33,71,236,283,295],"both":[30],"research":[31],"medical":[34],"diagnostics,":[35],"is":[36],"often":[37,69],"limited":[38],"by":[39],"nature":[41],"RTs":[44,55,87],"available.":[45],"secondary":[47],"structure":[48],"can":[49],"prove":[50,258],"problematic":[51],"where":[52],"mesophilic":[53],"retroviral":[54,219],"are":[56],"used":[57],"while":[58],"alternative":[60,289],"approach,":[61],"thermophilic":[63,86,105,290],"RT":[67,102,147,199,243,280],"activity,":[68],"results":[70],"error-prone":[72],"cDNA":[73,303,321],"production.":[74],"project":[76],"recognised":[77],"need":[79],"to":[80,145,165,196,241,259,262,319],"study":[81,91],"other":[82],"possible":[83,239],"sources":[84],"outlines":[89],"four":[93],"previously":[94],"uncharacterised":[95],"Group":[96],"II":[97],"Intronencoded":[98],"proteins":[99],"(IEP),":[100],"domains,":[103],"from":[104,137,161],"bacteria.":[106],"While":[107],"cloning":[108],"IEP":[111,160],"genes":[112],"their":[114],"expression":[115],"a":[117,129,183],"small":[118],"scale":[119],"proved":[120,164],"successful,":[121],"difficulties":[122],"were":[123,143,194],"encountered":[124],"when":[125],"attempting":[126],"purification.":[127],"Despite":[128],"lack":[130],"overall":[132],"purity,":[133],"samples":[134],"containing":[135],"IEPs":[136,153,222,293],"Thermosinus":[138],"carboxydivorans":[139],"Petrotoga":[141],"mobilis":[142],"shown":[144,195],"have":[146,197],"activity":[148,200,204,244],"but":[149],"characterisation":[150,171],"these":[152,213],"was":[154,175,179,233],"not":[155,257],"carried":[156],"out.":[157],"However,":[158,248],"Bacillus":[162],"caldovelox":[163],"be":[166,260],"excellent":[168],"candidate":[169],"for":[170],"as":[172,287],"successful":[173],"purification":[174],"achieved.":[176],"Enzyme":[177],"engineering":[178],"also":[180,234],"performed,":[181],"fusing":[182],"Sac7d":[184,230],"domain":[185,255],"onto":[186],"C-terminus":[188],"this":[190,254,277,296],"protein.":[191],"These":[192],"enzymes":[193,214],"optimum":[198,310],"at":[201,208],"54\u00baC":[202],"still":[205],"being":[206],"displayed":[207,223],"76\u00baC.":[209],"Other":[210],"studies":[211,250],"showed":[215,251],"that,":[216,252],"unlike":[217],"RTs,":[220],"no":[224],"DNA-dependent":[225],"polymerase":[227],"The":[229],"fusion":[231],"protein":[232],"studied":[235,294],"terms":[237,284],"enhancements":[240],"IEP.":[247],"preliminary":[249],"although":[253],"did":[256,298],"detrimental":[261],"enzyme,":[264],"it":[265],"had":[266],"little":[267],"effect":[268],"improving":[270],"processivity":[272],"RTs.":[275],"Although":[276],"class":[278],"looks":[281],"promising":[282],"use":[286],"RT,":[291],"report":[297],"incur":[299],"limitations":[301],"during":[302],"synthesis,":[304],"which":[305],"included":[306],"lower":[307],"than":[308],"expected":[309],"temperatures,":[312],"very":[313],"low":[314],"fidelity":[315],"inability":[318],"synthesise":[320],"complex":[323],"templates.":[325]},"counts_by_year":[],"updated_date":"2026-08-21T09:56:20.448147","created_date":"2016-06-24T00:00:00"}