{"id":"https://openalex.org/W2810692685","doi":null,"title":"CRISPR/Cas9-based genome editing in mice: state of the art and future perspectives","display_name":"CRISPR/Cas9-based genome editing in mice: state of the art and future perspectives","publication_year":2018,"publication_date":"2018-06-06","ids":{"openalex":"https://openalex.org/W2810692685","mag":"2810692685"},"language":"en","primary_location":{"id":"pmh:oai:invenio.nusl.cz:380887","doi":null,"is_oa":false,"landing_page_url":"http://www.nusl.cz/ntk/nusl-380887","pdf_url":null,"source":{"id":"https://openalex.org/S4306402266","display_name":"Digital Repository (National Repository of Grey Literature)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79620101","host_organization_name":"University of Arkansas for Medical Sciences","host_organization_lineage":["https://openalex.org/I79620101"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Jan Eli\u00e1\u0161","orcid":"https://orcid.org/0009-0006-8792-3968","observed_orcids":["https://orcid.org/0009-0006-8792-3968"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Jan Eli\u00e1\u0161","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0006-8792-3968","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9980000257492065},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.984000027179718},{"id":"https://openalex.org/keywords/mouse-mutants","display_name":"mouse mutants","score":0.7450000047683716},{"id":"https://openalex.org/keywords/gene-targeting","display_name":"gene targeting","score":0.5199999809265137},{"id":"https://openalex.org/keywords/homologous-recombination","display_name":"homologous recombination","score":0.8980000019073486},{"id":"https://openalex.org/keywords/transgenesis","display_name":"transgenesis","score":0.8610000014305115},{"id":"https://openalex.org/keywords/genetic-ablation","display_name":"gene ablation","score":0.7139999866485596},{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.7210000157356262},{"id":"https://openalex.org/keywords/programmable-nucleases","display_name":"programmable nucleases","score":0.7850000262260437},{"id":"https://openalex.org/keywords/embryonic-stem-cells","display_name":"embryonic stem cells","score":0.6639999747276306},{"id":"https://openalex.org/keywords/animal-models","display_name":"animal models","score":0.5080000162124634}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9225138425827026},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8343350291252136},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5042656660079956},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.47471606731414795},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.4629192650318146},{"id":"https://openalex.org/C48103436","wikidata":"https://www.wikidata.org/wiki/Q599031","display_name":"State (computer science)","level":2,"score":0.4600197672843933},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4437849819660187},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4420396685600281},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2944689095020294},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.22037392854690552},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17677909135818481}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:invenio.nusl.cz:380887","doi":null,"is_oa":false,"landing_page_url":"http://www.nusl.cz/ntk/nusl-380887","pdf_url":null,"source":{"id":"https://openalex.org/S4306402266","display_name":"Digital Repository (National Repository of Grey Literature)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79620101","host_organization_name":"University of Arkansas for Medical Sciences","host_organization_lineage":["https://openalex.org/I79620101"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},{"id":"pmh:oai:invenio.nusl.cz:464769","doi":null,"is_oa":false,"landing_page_url":"http://www.nusl.cz/ntk/nusl-464769","pdf_url":null,"source":{"id":"https://openalex.org/S4306402266","display_name":"Digital Repository (National Repository of Grey Literature)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I79620101","host_organization_name":"University of Arkansas for Medical Sciences","host_organization_lineage":["https://openalex.org/I79620101"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/bachelorThesis"},{"id":"mag:2810692685","doi":null,"is_oa":false,"landing_page_url":"https://dspace.cuni.cz/handle/20.500.11956/98669","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2390994276","https://openalex.org/W2294897294","https://openalex.org/W2110565227","https://openalex.org/W2917268350","https://openalex.org/W2257147990","https://openalex.org/W2259042290","https://openalex.org/W2149107615","https://openalex.org/W2781470625","https://openalex.org/W2798156675","https://openalex.org/W2529467023"],"abstract_inverted_index":{"Mutant":[0],"mice":[1,137],"are":[2,116],"crucial":[3],"tools":[4],"for":[5],"understanding":[6],"gene":[7,104],"functions":[8],"in":[9,133],"vivo.":[10],"Recently,":[11],"generation":[12,46],"of":[13,21,34,47,61,68,79,88,96,102,109,128,135,141,148],"mouse":[14,40],"mutants":[15],"was":[16],"revolutionized":[17],"by":[18,25,85,93],"rapid":[19],"developement":[20],"programmable":[22],"nucleases,":[23],"predominantly":[24],"the":[26,55,89,97,103,142,146],"CRISPR/Cas9":[27,35,69,129],"system.":[28],"Genome":[29],"editing":[30],"based":[31,58],"on":[32,59],"introduction":[33],"components":[36],"into":[37,113],"early":[38],"stage":[39],"embyros":[41],"allows":[42],"fast":[43],"and":[44,138],"inexpensive":[45],"gene-deficient":[48],"animal":[49],"models,":[50],"especially":[51],"when":[52],"compared":[53],"to":[54,70,119,144],"traditional":[56],"techniques":[57],"modification":[60,87,101],"embryonic":[62],"stem":[63],"cells":[64],"(ESCs).":[65],"The":[66],"ability":[67],"induce":[71],"double-strand":[72],"break":[73],"(DSB)":[74],"at":[75],"a":[76,110,126],"given":[77],"location":[78],"genomic":[80],"DNA":[81,111],"enables":[82],"effective":[83],"gene-ablation":[84],"random":[86],"coding":[90],"sequences":[91],"or":[92],"complete":[94],"ablation":[95],"gene.":[98],"However,":[99],"precise":[100,149],"sequences,":[105],"such":[106],"as":[107],"incorporation":[108],"fragment":[112],"specific":[114],"loci,":[115],"still":[117],"difficult":[118],"make.":[120],"In":[121],"this":[122],"work,":[123],"I":[124],"present":[125],"review":[127],"system,":[130],"its":[131],"use":[132],"production":[134],"mutant":[136],"possible":[139],"modifications":[140],"system":[143],"increase":[145],"efficiency":[147],"gene-targeting.":[150],"Keywords:":[151],"CRISPR/Cas9,":[152],"mouse,":[153],"transgenesis,":[154],"homologous":[155],"recombination":[156]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2018-07-10T00:00:00"}