{"id":"https://openalex.org/W2808948286","doi":"https://doi.org/10.2144/btn-2018-0012","title":"A PAGE screening approach for identifying CRISPR-Cas9-induced mutations in zebrafish","display_name":"A PAGE screening approach for identifying CRISPR-Cas9-induced mutations in zebrafish","publication_year":2018,"publication_date":"2018-06-01","ids":{"openalex":"https://openalex.org/W2808948286","doi":"https://doi.org/10.2144/btn-2018-0012","mag":"2808948286","pmid":"https://pubmed.ncbi.nlm.nih.gov/29939088","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/9580339"},"language":"en","primary_location":{"id":"doi:10.2144/btn-2018-0012","doi":"https://doi.org/10.2144/btn-2018-0012","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2018-0012","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/btn-2018-0012","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068213512","display_name":"Ariel J. VanLeuven","orcid":"https://orcid.org/0000-0002-7725-9534","observed_orcids":["https://orcid.org/0000-0002-7725-9534"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ariel J VanLeuven","raw_affiliation_strings":["Department of Cellular Biology, University of Georgia, 724 Biological Sciences Building, Athens, GA 30602-2607, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular Biology, University of Georgia, 724 Biological Sciences Building, Athens, GA 30602-2607, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075211129","display_name":"Sungdae Park","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sungdae Park","raw_affiliation_strings":["Department of Genetics, University of Georgia, Paul D Coverdell Center, 500 D.W. Brooks Drive, Room 250, Athens, GA 30602, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, University of Georgia, Paul D Coverdell Center, 500 D.W. Brooks Drive, Room 250, Athens, GA 30602, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019780225","display_name":"Douglas B. Menke","orcid":"https://orcid.org/0000-0002-7109-1451","observed_orcids":["https://orcid.org/0000-0002-7109-1451"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Douglas B Menke","raw_affiliation_strings":["Department of Genetics, University of Georgia, Paul D Coverdell Center, 500 D.W. Brooks Drive, Room 250, Athens, GA 30602, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics, University of Georgia, Paul D Coverdell Center, 500 D.W. Brooks Drive, Room 250, Athens, GA 30602, USA","institution_ids":["https://openalex.org/I165733156"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069049550","display_name":"James D. Lauderdale","orcid":"https://orcid.org/0000-0001-7503-0528","observed_orcids":["https://orcid.org/0000-0001-7503-0528"]},"institutions":[{"id":"https://openalex.org/I165733156","display_name":"University of Georgia","ror":"https://ror.org/00te3t702","country_code":"US","type":"education","lineage":["https://openalex.org/I165733156"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James D Lauderdale","raw_affiliation_strings":["Department of Cellular Biology,University of Georgia,\r724 Biological Sciences Building,\rAthens,\rGA 30602-2607,\rUSA","Department of Genetics, University of Georgia,Paul D Coverdell Center,\r500 D.W. Brooks Drive, Room 250,\rAthens,\rGA 30602,\rUSA","Faculty in the Neuroscience Division of the Biomedical & Health Sciences Institute (BHSI),University of Georgia,Paul D Coverdell Center,\r500 D.W. Brooks Drive,\rAthens,\rGA 30602-7394,\rUSA","Fax: +1 706 542 4271"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cellular Biology,University of Georgia,\r724 Biological Sciences Building,\rAthens,\rGA 30602-2607,\rUSA","institution_ids":["https://openalex.org/I165733156"]},{"raw_affiliation_string":"Department of Genetics, University of Georgia,Paul D Coverdell Center,\r500 D.W. Brooks Drive, Room 250,\rAthens,\rGA 30602,\rUSA","institution_ids":["https://openalex.org/I165733156"]},{"raw_affiliation_string":"Faculty in the Neuroscience Division of the Biomedical & Health Sciences Institute (BHSI),University of Georgia,Paul D Coverdell Center,\r500 D.W. Brooks Drive,\rAthens,\rGA 30602-7394,\rUSA","institution_ids":["https://openalex.org/I165733156"]},{"raw_affiliation_string":"Fax: +1 706 542 4271","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I165733156"],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":0.8,"has_fulltext":false,"cited_by_count":18,"citation_normalized_percentile":{"value":0.69208853,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"64","issue":"6","first_page":"275","last_page":"278"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11685","display_name":"Zebrafish Biomedical Research Applications","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9739999771118164},{"id":"https://openalex.org/keywords/zebrafish","display_name":"zebrafish","score":0.8220000267028809},{"id":"https://openalex.org/keywords/mutation-detection","display_name":"mutation detection","score":0.6610000133514404},{"id":"https://openalex.org/keywords/pcr-based-genotyping","display_name":"PCR genotyping","score":0.7630000114440918},{"id":"https://openalex.org/keywords/targeted-mutagenesis","display_name":"targeted mutagenesis","score":0.8309999704360962},{"id":"https://openalex.org/keywords/heteroduplex-detection","display_name":"heteroduplex detection","score":0.7360000014305115},{"id":"https://openalex.org/keywords/reverse-genetics","display_name":"reverse genetics","score":0.5809999704360962},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.8009999990463257}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9190511703491211},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6904488205909729},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.669156014919281},{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.6688548922538757},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5624389052391052},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5459164977073669},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.527100682258606},{"id":"https://openalex.org/C64869954","wikidata":"https://www.wikidata.org/wiki/Q1859747","display_name":"False positive paradox","level":2,"score":0.4923802614212036},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.4878070652484894},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3741663098335266},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.2809094786643982},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19479256868362427},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.1729409098625183},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0634111762046814}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015027","descriptor_name":"Zebrafish","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D060005","descriptor_name":"Genotyping Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D063347","descriptor_name":"Native Polyacrylamide Gel Electrophoresis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.2144/btn-2018-0012","doi":"https://doi.org/10.2144/btn-2018-0012","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2018-0012","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:29939088","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29939088","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:9580339","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9580339","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Biotechniques","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:95be96648bf74e28b4770c05d12d9f9c","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/95be96648bf74e28b4770c05d12d9f9c","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 64, Iss 6, Pp 275-278 (2018)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/btn-2018-0012","doi":"https://doi.org/10.2144/btn-2018-0012","is_oa":true,"landing_page_url":"https://doi.org/10.2144/btn-2018-0012","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1093913137","display_name":"CRCNS: Quantum of Network Dysfunction in Epilepsy-Understanding the Inhibitory Restraint","funder_award_id":"R01NS090645","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G5386797595","display_name":"Developmental context and the control of appendage cis-regulatory networks","funder_award_id":"R01HD081034","funder_id":"https://openalex.org/F4320337611","funder_display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development"}],"funders":[{"id":"https://openalex.org/F4320310010","display_name":"University of Georgia","ror":"https://ror.org/00te3t702"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"},{"id":"https://openalex.org/F4320337611","display_name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","ror":"https://ror.org/04byxyr05"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":16,"referenced_works":["https://openalex.org/W1912701812","https://openalex.org/W1970549334","https://openalex.org/W1998416188","https://openalex.org/W2003911538","https://openalex.org/W2008205228","https://openalex.org/W2017224987","https://openalex.org/W2031120035","https://openalex.org/W2048010932","https://openalex.org/W2134095170","https://openalex.org/W2215279579","https://openalex.org/W2343277111","https://openalex.org/W2473953377","https://openalex.org/W2494669249","https://openalex.org/W2604748431","https://openalex.org/W3215043601","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2305674065","https://openalex.org/W7097512085","https://openalex.org/W2604748431","https://openalex.org/W2907490082","https://openalex.org/W2031120035","https://openalex.org/W2087875447","https://openalex.org/W4210907391","https://openalex.org/W3199069219","https://openalex.org/W2215279579","https://openalex.org/W2497831899"],"abstract_inverted_index":{"The":[0],"introduction":[1],"of":[2,24,37,81],"CRISPR-Cas9":[3,44],"technology":[4],"for":[5,32,42],"targeted":[6],"mutagenesis":[7],"has":[8],"revolutionized":[9],"reverse":[10],"genetics":[11],"and":[12,83,110],"made":[13],"genome":[14],"editing":[15],"a":[16,66,115],"realistic":[17],"option":[18],"in":[19,34,50,93],"many":[20],"model":[21],"organisms.":[22],"One":[23],"the":[25,79,98],"difficulties":[26],"with":[27],"this":[28],"technique":[29],"is":[30,111],"screening":[31,40,68],"mutations":[33,109],"large":[35],"numbers":[36],"samples.":[38],"Many":[39],"approaches":[41],"identifying":[43],"mutants":[45],"have":[46],"been":[47],"published;":[48],"however,":[49],"practice":[51],"these":[52],"methods":[53],"are":[54],"time":[55],"consuming,":[56],"expensive,":[57],"or":[58],"often":[59],"yield":[60],"false":[61],"positives.":[62],"This":[63,73,101],"report":[64],"describes":[65],"PCR-based":[67],"approach":[69,74,102],"using":[70],"non-denaturing":[71],"PAGE.":[72],"does":[75],"not":[76],"depend":[77],"on":[78],"formation":[80],"heteroduplexes":[82],"reliably":[84],"detects":[85],"changes":[86],"as":[87,89,114],"small":[88],"1":[90],"base-pair":[91],"(bp)":[92],"nucleic":[94],"acid":[95],"length":[96],"at":[97],"target":[99],"site.":[100],"can":[103],"be":[104],"used":[105],"to":[106],"identify":[107],"novel":[108],"also":[112],"useful":[113],"routine":[116],"genotyping":[117],"method.":[118]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-06-29T00:00:00"}