{"id":"https://openalex.org/W2808530735","doi":null,"title":"The characterization of 17\u03b2-hydroxysteroid dehydrogenase type 2 (17\u03b2HSD2) activity towards novel C19 substrates.","display_name":"The characterization of 17\u03b2-hydroxysteroid dehydrogenase type 2 (17\u03b2HSD2) activity towards novel C19 substrates.","publication_year":2017,"publication_date":"2017-03-01","ids":{"openalex":"https://openalex.org/W2808530735","mag":"2808530735"},"language":"en","primary_location":{"id":"pmh:oai:scholar.sun.ac.za:10019.1/101459","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/101459","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5072116309","display_name":"Monique Barnard","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Monique Barnard","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5072116309"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.9491999745368958,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.9491999745368958,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13736","display_name":"Steroid Chemistry and Biochemistry","score":0.021900000050663948,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11146","display_name":"Hormonal and reproductive studies","score":0.005100000184029341,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/17beta-hydroxysteroid-dehydrogenase-type-2","display_name":"17\u03b2-hydroxysteroid dehydrogenase type 2","score":0.984000027179718},{"id":"https://openalex.org/keywords/akr1c3","display_name":"AKR1C3","score":0.9340000152587891},{"id":"https://openalex.org/keywords/11-ketotestosterone","display_name":"11-ketotestosterone","score":0.9079999923706055},{"id":"https://openalex.org/keywords/androgen-metabolism","display_name":"androgen metabolism","score":0.6639999747276306},{"id":"https://openalex.org/keywords/castration-resistant-prostate-cancer","display_name":"castration-resistant prostate cancer","score":0.9490000009536743},{"id":"https://openalex.org/keywords/enzyme-catalytic-efficiency","display_name":"enzyme catalytic efficiency","score":0.5770000219345093},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.515999972820282},{"id":"https://openalex.org/keywords/hek293-cells","display_name":"HEK293 cells","score":0.6819999814033508},{"id":"https://openalex.org/keywords/transient-transfection","display_name":"transient transfection","score":0.5239999890327454}],"concepts":[{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.5868426561355591},{"id":"https://openalex.org/C2780841128","wikidata":"https://www.wikidata.org/wiki/Q5073781","display_name":"Characterization (materials science)","level":2,"score":0.5193729996681213},{"id":"https://openalex.org/C2781067048","wikidata":"https://www.wikidata.org/wiki/Q5955660","display_name":"Hydroxysteroid dehydrogenase","level":4,"score":0.467923641204834},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3594164252281189},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3180893063545227},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.21035701036453247},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.19762766361236572},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.19097113609313965}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholar.sun.ac.za:10019.1/101459","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/101459","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2808530735","doi":null,"is_oa":false,"landing_page_url":"https://scholar.sun.ac.za/handle/10019.1/101459","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7246000170707703}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2809641829","https://openalex.org/W2171708792","https://openalex.org/W1977666529","https://openalex.org/W2916717528","https://openalex.org/W1976460651","https://openalex.org/W2060524703","https://openalex.org/W2027697866","https://openalex.org/W2501835417","https://openalex.org/W2006788503","https://openalex.org/W6982552354"],"abstract_inverted_index":{"ENGLISH":[0],"ABSTRACT:":[1],"Castration":[2],"resistant":[3],"prostate":[4,374],"cancer":[5,375],"(CRPC)":[6],"is":[7,87],"an":[8,395],"androgen":[9,19,39,120,401],"dependent":[10],"disease":[11],"driven":[12],"by":[13],"the":[14,29,37,42,49,54,75,93,116,130,138,144,154,157,166,175,193,204,212,220,265,291,304,307,312,325,348,364,385,409,412],"intratumoural":[15,400],"metabolism":[16],"of":[17,77,95,108,118,124,132,146,149,195,214,222,234,237,283,297,314,341,350],"adrenal":[18,30,50],"precursors":[20],"to":[21,36,79,98,128,142,183,199,208,217,224,260,285,311,338,345,352],"potent":[22,38,55,100,316],"androgens.":[23,101],"The":[24,102,122,186,232],"alternative":[25,158],"5\u03b1-dione":[26,159],"pathway":[27,47,140],"converts":[28,48],"steroids":[31,136,168],"DHEA":[32],"and":[33,59,105,141,151,160,170,197,264,334,362,403],"androstenedione":[34],"(A4)":[35],"DHT,":[40],"while":[41,84,347],"recently":[43],"identified":[44],"11\u03b2-hydroxyandrostenedione":[45],"(11OHA4)":[46],"steroid":[51],"11OHA4":[52,139,161,308,413],"into":[53],"androgens":[56,78,97],"11-ketotestosterone":[57],"(11KT)":[58],"11-ketodihydrotestosterone":[60],"(11KDHT).":[61],"Two":[62],"17\u03b2-hydroxysteroid":[63],"dehydrogenase":[64],"(17\u03b2HSD)":[65],"enzymes":[66,110],"catalyse":[67],"vital":[68,113],"reactions":[69],"in":[70,114,269,331,372,398],"both":[71,332],"pathways.":[72,162,327],"17\u03b2HSD2":[73,133,152,163,190,392],"catalyses":[74,92],"oxidation":[76],"their":[80,200],"less":[81],"active":[82,119],"form,":[83],"17\u03b2HSD5,":[85],"which":[86,239,248,274],"better":[88],"known":[89],"as":[90],"AKR1C3,":[91],"reduction":[94],"weak":[96],"more":[99,315],"relative":[103],"activity":[104,131,164],"expression":[106,296,330],"levels":[107,340,402],"these":[109],"are":[111],"therefore":[112],"regulating":[115,399],"amount":[117],"produced.":[121],"aim":[123],"this":[125,227,388],"project":[126],"was":[127,229,359],"characterise":[129],"towards":[134,165],"11-oxygenated":[135,167],"from":[137,387],"investigate":[143],"effect":[145,233,323],"different":[147,270,287,380],"ratios":[148,236,407],"AKR1C3":[150,254,284,298],"on":[153,303,324],"flux":[155,305,410],"through":[156,306,411],"11KT":[169,196,223,346],"11KDHT":[171,198],"were":[172,243,257,278,369],"characterised":[173],"for":[174,211],"first":[176],"time":[177],"using":[178],"HEK293":[179,246,333],"cells":[180,266,336],"transiently":[181,258],"transfected":[182,259,279],"express":[184,252,261,276],"17\u03b2HSD2.":[185],"results":[187,292,368,386],"showed":[188,293],"that":[189,294,391,404],"efficiently":[191],"catalysed":[192],"conversion":[194,213,221,349],"respective":[201],"products.":[202],"Interestingly":[203],"catalytic":[205],"efficiency":[206],"tended":[207],"be":[209],"higher":[210],"testosterone":[215],"(T)":[216],"A4":[218,351],"than":[219],"11KA4,":[225],"although":[226],"difference":[228],"not":[230,250],"significant.":[231],"increasing":[235,281],"AKR1C3:17\u03b2HSD2,":[238],"occur":[240],"during":[241],"CRPC,":[242],"subsequently":[244,267,360],"investigated.":[245],"cells,":[247,273],"do":[249],"endogenously":[251,275],"either":[253],"or":[255,321],"17\u03b2HSD2,":[256,277],"each":[262,378],"enzyme,":[263],"combined":[268],"ratios.":[271,289,382],"PC3":[272,335],"with":[280],"amounts":[282],"obtain":[286],"AKR1C3:17\u03b2HSD2":[288,329,381,406],"Collectively":[290],"increased":[295,339,405],"had":[299,319],"a":[300],"significant":[301],"influence":[302],"pathway,":[309],"leading":[310],"production":[313],"androgens,":[317],"but":[318],"little":[320],"no":[322],"classical":[326],"Increasing":[328],"lead":[337],"11KA4":[342],"being":[343],"converted":[344],"T":[353],"remained":[354],"low.":[355],"A":[356],"mathematical":[357],"model":[358],"constructed":[361],"confirmed":[363],"experimental":[365],"findings.":[366],"These":[367],"further":[370],"validated":[371],"three":[373],"cell":[376],"lines":[377],"expressing":[379],"Taken":[383],"together,":[384],"study":[389],"show":[390],"likely":[393],"plays":[394],"important":[396],"role":[397],"favour":[408],"pathway.":[414]},"counts_by_year":[],"updated_date":"2026-08-18T07:49:30.821534","created_date":"2018-06-21T00:00:00"}