{"id":"https://openalex.org/W2807445467","doi":"https://doi.org/10.1002/biot.201700596","title":"Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering","display_name":"Targeted Nucleotide Editing Technologies for Microbial Metabolic Engineering","publication_year":2018,"publication_date":"2018-06-04","ids":{"openalex":"https://openalex.org/W2807445467","doi":"https://doi.org/10.1002/biot.201700596","mag":"2807445467","pmid":"https://pubmed.ncbi.nlm.nih.gov/29862665"},"language":"en","primary_location":{"id":"doi:10.1002/biot.201700596","doi":"https://doi.org/10.1002/biot.201700596","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201700596","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085763416","display_name":"Takayuki Arazoe","orcid":"https://orcid.org/0000-0001-8804-315X","observed_orcids":["https://orcid.org/0000-0001-8804-315X"]},"institutions":[{"id":"https://openalex.org/I161296585","display_name":"Tokyo University of Science","ror":"https://ror.org/05sj3n476","country_code":"JP","type":"education","lineage":["https://openalex.org/I161296585"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takayuki Arazoe","raw_affiliation_strings":["Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Applied Biological Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510 Japan","institution_ids":["https://openalex.org/I161296585"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014256354","display_name":"Akihiko Kondo","orcid":"https://orcid.org/0000-0003-1527-5288","observed_orcids":["https://orcid.org/0000-0003-1527-5288"]},"institutions":[{"id":"https://openalex.org/I65837984","display_name":"Kobe University","ror":"https://ror.org/03tgsfw79","country_code":"JP","type":"education","lineage":["https://openalex.org/I65837984"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Akihiko Kondo","raw_affiliation_strings":["Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan","Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan","institution_ids":["https://openalex.org/I65837984"]},{"raw_affiliation_string":"Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan","institution_ids":["https://openalex.org/I65837984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037549250","display_name":"Keiji Nishida","orcid":"https://orcid.org/0000-0002-9371-4971","observed_orcids":["https://orcid.org/0000-0002-9371-4971"]},"institutions":[{"id":"https://openalex.org/I65837984","display_name":"Kobe University","ror":"https://ror.org/03tgsfw79","country_code":"JP","type":"education","lineage":["https://openalex.org/I65837984"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Keiji Nishida","raw_affiliation_strings":["Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501 Japan","institution_ids":["https://openalex.org/I65837984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5014256354","https://openalex.org/A5037549250"],"corresponding_institution_ids":["https://openalex.org/I65837984"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":null,"fwci":0.6152,"has_fulltext":false,"cited_by_count":54,"citation_normalized_percentile":{"value":0.52733473,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"13","issue":"9","first_page":"e1700596","last_page":"e1700596"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.0020000000949949026,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/base-editing","display_name":"base editing","score":0.6869999766349792},{"id":"https://openalex.org/keywords/crispr-cas-systems","display_name":"CRISPR-Cas","score":0.8019999861717224},{"id":"https://openalex.org/keywords/microbial-metabolic-engineering","display_name":"microbial metabolic engineering","score":0.7509999871253967},{"id":"https://openalex.org/keywords/deaminase","display_name":"deaminase","score":0.703000009059906},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.45899999141693115},{"id":"https://openalex.org/keywords/prokaryotes","display_name":"prokaryotes","score":0.718999981880188},{"id":"https://openalex.org/keywords/recombineering","display_name":"recombineering","score":0.8090000152587891},{"id":"https://openalex.org/keywords/negative-selection","display_name":"negative selection","score":0.5519999861717224}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.9207468628883362},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8810449838638306},{"id":"https://openalex.org/C70239388","wikidata":"https://www.wikidata.org/wiki/Q920203","display_name":"Recombineering","level":4,"score":0.8109172582626343},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.653045654296875},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.619679868221283},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5773727297782898},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5566907525062561},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.495707631111145},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.49339452385902405},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.4928124248981476},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.42481809854507446}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D053903","descriptor_name":"DNA Breaks, Double-Stranded","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/biot.201700596","doi":"https://doi.org/10.1002/biot.201700596","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201700596","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},{"id":"pmid:29862665","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29862665","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology journal","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2130288986","display_name":"Construction of filamentous fungal artificial chromosome and analysis of horizontal transfer and pathogenicity evolution mechanisms in the tomato wilt fungus.","funder_award_id":"18K19218","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G598213413","display_name":"Creation of self-evolving enzymatic system that enables semi rational design of genome","funder_award_id":"16K14654","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6132995996","display_name":"Integral analysis of infection and mutation mechanisms in the rice blast fungus","funder_award_id":"17H05021","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G6620779292","display_name":null,"funder_award_id":"26119710","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G96564276","display_name":"Creation of artificial enzymes that enable targeted editing of any DNA bases","funder_award_id":"17H04994","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320320912","display_name":"Ministry of Education, Culture, Sports, Science and Technology","ror":"https://ror.org/048rj2z13"},{"id":"https://openalex.org/F4320321680","display_name":"New Energy and Industrial Technology Development Organization","ror":"https://ror.org/0055k7a87"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":133,"referenced_works":["https://openalex.org/W193111756","https://openalex.org/W826124742","https://openalex.org/W1483198897","https://openalex.org/W1540505601","https://openalex.org/W1580639168","https://openalex.org/W1608084731","https://openalex.org/W1870558638","https://openalex.org/W1922040927","https://openalex.org/W1980262173","https://openalex.org/W1991818214","https://openalex.org/W2000004958","https://openalex.org/W2000046950","https://openalex.org/W2003171404","https://openalex.org/W2010659234","https://openalex.org/W2013532225","https://openalex.org/W2021688572","https://openalex.org/W2023600884","https://openalex.org/W2044022101","https://openalex.org/W2052024371","https://openalex.org/W2059223148","https://openalex.org/W2064815984","https://openalex.org/W2068427957","https://openalex.org/W2071103917","https://openalex.org/W2096261947","https://openalex.org/W2116137883","https://openalex.org/W2119633529","https://openalex.org/W2120263648","https://openalex.org/W2120932131","https://openalex.org/W2121695681","https://openalex.org/W2123060905","https://openalex.org/W2127862779","https://openalex.org/W2136670217","https://openalex.org/W2139891717","https://openalex.org/W2143999510","https://openalex.org/W2144779133","https://openalex.org/W2145782793","https://openalex.org/W2155208047","https://openalex.org/W2156252266","https://openalex.org/W2160051211","https://openalex.org/W2165608602","https://openalex.org/W2165869860","https://openalex.org/W2174662127","https://openalex.org/W2199695484","https://openalex.org/W2204786127","https://openalex.org/W2234629144","https://openalex.org/W2268557312","https://openalex.org/W2273981063","https://openalex.org/W2282184745","https://openalex.org/W2293809279","https://openalex.org/W2312592272","https://openalex.org/W2313697469","https://openalex.org/W2327895535","https://openalex.org/W2336828812","https://openalex.org/W2341319313","https://openalex.org/W2343655376","https://openalex.org/W2347725975","https://openalex.org/W2397457481","https://openalex.org/W2399213131","https://openalex.org/W2410277937","https://openalex.org/W2413011000","https://openalex.org/W2416098422","https://openalex.org/W2416874763","https://openalex.org/W2433199239","https://openalex.org/W2470889463","https://openalex.org/W2472136907","https://openalex.org/W2480829753","https://openalex.org/W2498972343","https://openalex.org/W2527387613","https://openalex.org/W2529420003","https://openalex.org/W2530994846","https://openalex.org/W2542621333","https://openalex.org/W2550728222","https://openalex.org/W2553845783","https://openalex.org/W2558343263","https://openalex.org/W2560199174","https://openalex.org/W2560407750","https://openalex.org/W2565169990","https://openalex.org/W2579839460","https://openalex.org/W2583283187","https://openalex.org/W2586637020","https://openalex.org/W2588419087","https://openalex.org/W2588556918","https://openalex.org/W2590355459","https://openalex.org/W2593264565","https://openalex.org/W2594473698","https://openalex.org/W2594532403","https://openalex.org/W2594683972","https://openalex.org/W2598674316","https://openalex.org/W2605598748","https://openalex.org/W2607273379","https://openalex.org/W2607417244","https://openalex.org/W2609834193","https://openalex.org/W2610391087","https://openalex.org/W2610984386","https://openalex.org/W2613730347","https://openalex.org/W2621647941","https://openalex.org/W2622049469","https://openalex.org/W2626905039","https://openalex.org/W2673445789","https://openalex.org/W2713682953","https://openalex.org/W2736435199","https://openalex.org/W2737091810","https://openalex.org/W2738205911","https://openalex.org/W2740482107","https://openalex.org/W2744490757","https://openalex.org/W2745718557","https://openalex.org/W2747777260","https://openalex.org/W2750827051","https://openalex.org/W2751474071","https://openalex.org/W2752680598","https://openalex.org/W2752929029","https://openalex.org/W2753116183","https://openalex.org/W2755256267","https://openalex.org/W2761894131","https://openalex.org/W2765214919","https://openalex.org/W2765938576","https://openalex.org/W2766599608","https://openalex.org/W2769160949","https://openalex.org/W2769413246","https://openalex.org/W2769919647","https://openalex.org/W2789818578","https://openalex.org/W2790533646","https://openalex.org/W2793309516","https://openalex.org/W2924195738","https://openalex.org/W2946567891","https://openalex.org/W2950497661","https://openalex.org/W2950826032","https://openalex.org/W2952813308","https://openalex.org/W2969197547","https://openalex.org/W2989710008","https://openalex.org/W2994523664","https://openalex.org/W4238526656","https://openalex.org/W4245053291"],"related_works":["https://openalex.org/W3112489873","https://openalex.org/W3042827624","https://openalex.org/W7037042252","https://openalex.org/W4224294434","https://openalex.org/W4409632689","https://openalex.org/W2015607962","https://openalex.org/W2789818578","https://openalex.org/W4381325555","https://openalex.org/W7165022014","https://openalex.org/W3148614975"],"abstract_inverted_index":{"Since":[0],"the":[1,65,84,132,141,146],"emergence":[2],"of":[3,134,173],"programmable":[4],"RNA-guided":[5],"nucleases":[6],"based":[7],"on":[8,131],"clustered":[9],"regularly":[10],"interspaced":[11],"short":[12],"palindromic":[13],"repeats":[14],"(CRISPR)/CRISPR-associated":[15],"protein":[16],"(Cas)":[17],"systems,":[18],"genome":[19,30,39,43,152],"editing":[20,31,40,156],"technologies":[21,106,129,166],"have":[22,107],"become":[23],"a":[24,91],"simplified":[25],"and":[26,35,51,104,118,124,149,158],"versatile":[27],"tool":[28],"for":[29],"in":[32,60,77,95,170,183],"various":[33,171],"organisms":[34],"cell":[36],"types.":[37],"Although":[38],"enables":[41],"efficient":[42],"manipulations,":[44],"such":[45],"as":[46,90],"gene":[47,49],"disruptions,":[48],"knockins,":[50],"chromosomal":[52],"translocations":[53],"via":[54],"DNA":[55,126],"double-strand":[56],"break":[57],"(DSB)":[58],"repair":[59],"eukaryotes,":[61],"DSBs":[62,123],"induced":[63],"by":[64],"CRISPR/Cas":[66,85,143],"system":[67,86],"are":[68],"lethal":[69],"or":[70,99],"severely":[71],"toxic":[72],"to":[73,111,116,120,177,180],"many":[74,78],"microorganisms.":[75],"Therefore,":[76],"prokaryotes,":[79],"including":[80],"industrially":[81],"useful":[82],"microbes,":[83],"is":[87],"often":[88],"used":[89],"negative":[92],"selection":[93],"component":[94],"combination":[96],"with":[97],"recombineering":[98],"other":[100],"related":[101],"strategies.":[102],"Novel":[103],"revolutionary":[105],"been":[108],"recently":[109],"developed":[110],"re-write":[112],"targeted":[113],"nucleotides":[114],"(C:G":[115],"T:A":[117],"A:T":[119],"G:C)":[121],"without":[122],"donor":[125],"templates.":[127],"These":[128],"rely":[130],"recruitment":[133],"deaminases":[135],"at":[136],"specific":[137],"target":[138],"loci":[139],"using":[140],"nuclease-deficient":[142],"system.":[144],"Here,":[145],"authors":[147,162],"review":[148],"compare":[150],"CRISPR-based":[151],"editing,":[153],"current":[154],"base":[155],"platforms":[157],"their":[159],"spectra.":[160],"The":[161],"discuss":[163],"how":[164],"these":[165],"can":[167],"be":[168],"applied":[169],"aspects":[172],"microbial":[174],"metabolic":[175],"engineering":[176],"overcome":[178],"barriers":[179],"cellular":[181],"regulation":[182],"prokaryotes.":[184]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":10},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2018-06-13T00:00:00"}