{"id":"https://openalex.org/W2806478209","doi":"https://doi.org/10.1088/1755-1315/157/1/012044","title":"Effect of selection agents to Chrysanthemum (Chrysanthemum morifolium) callus growth after Agrobacterium-mediated genetic transformation","display_name":"Effect of selection agents to Chrysanthemum (Chrysanthemum morifolium) callus growth after Agrobacterium-mediated genetic transformation","publication_year":2018,"publication_date":"2018-05-01","ids":{"openalex":"https://openalex.org/W2806478209","doi":"https://doi.org/10.1088/1755-1315/157/1/012044","mag":"2806478209"},"language":"en","primary_location":{"id":"doi:10.1088/1755-1315/157/1/012044","doi":"https://doi.org/10.1088/1755-1315/157/1/012044","is_oa":true,"landing_page_url":"https://doi.org/10.1088/1755-1315/157/1/012044","pdf_url":"https://iopscience.iop.org/article/10.1088/1755-1315/157/1/012044/pdf","source":{"id":"https://openalex.org/S4210195883","display_name":"IOP Conference Series Earth and Environmental Science","issn_l":"1755-1315","issn":["1755-1315","1755-1307"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320083","host_organization_name":"IOP Publishing","host_organization_lineage":["https://openalex.org/P4310320083","https://openalex.org/P4310311669"],"host_organization_lineage_names":["IOP Publishing","Institute of Physics"],"type":"conference"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IOP Conference Series: Earth and Environmental Science","raw_type":"journal-article"},"volume":null,"type":"conference-paper","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://iopscience.iop.org/article/10.1088/1755-1315/157/1/012044/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5013633593","display_name":"Rinaldi Sjahril","orcid":"https://orcid.org/0000-0001-8645-4828","observed_orcids":["https://orcid.org/0000-0001-8645-4828"]},"institutions":[{"id":"https://openalex.org/I98301867","display_name":"Hasanuddin University","ror":"https://ror.org/00da1gf19","country_code":"ID","type":"education","lineage":["https://openalex.org/I98301867"]}],"countries":["ID"],"is_corresponding":true,"raw_author_name":"R Sjahril","raw_affiliation_strings":["Laboratory of Plant Biosciences and Reproduction Biotechnology, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratory of Plant Biosciences and Reproduction Biotechnology, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia","institution_ids":["https://openalex.org/I98301867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113957676","display_name":"I Jamaluddin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98301867","display_name":"Hasanuddin University","ror":"https://ror.org/00da1gf19","country_code":"ID","type":"education","lineage":["https://openalex.org/I98301867"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"I Jamaluddin","raw_affiliation_strings":["Agro-technology, Graduate School of Agriculture, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Agro-technology, Graduate School of Agriculture, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia","institution_ids":["https://openalex.org/I98301867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113951252","display_name":"M Nadir","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98301867","display_name":"Hasanuddin University","ror":"https://ror.org/00da1gf19","country_code":"ID","type":"education","lineage":["https://openalex.org/I98301867"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"M Nadir","raw_affiliation_strings":["Animal Feed Chemistry Laboratory, Faculty of Animal Husbandry, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Animal Feed Chemistry Laboratory, Faculty of Animal Husbandry, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia","institution_ids":["https://openalex.org/I98301867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022749451","display_name":"Asman Asman","orcid":"https://orcid.org/0000-0001-6405-2844","observed_orcids":["https://orcid.org/0000-0001-6405-2844"]},"institutions":[{"id":"https://openalex.org/I98301867","display_name":"Hasanuddin University","ror":"https://ror.org/00da1gf19","country_code":"ID","type":"education","lineage":["https://openalex.org/I98301867"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"Asman","raw_affiliation_strings":["Plant Pest and Disease Laboratory, Faculty of Agriculture, Hasanuddin University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Pest and Disease Laboratory, Faculty of Agriculture, Hasanuddin University","institution_ids":["https://openalex.org/I98301867"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059635222","display_name":"Novaty Eny Dungga","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98301867","display_name":"Hasanuddin University","ror":"https://ror.org/00da1gf19","country_code":"ID","type":"education","lineage":["https://openalex.org/I98301867"]}],"countries":["ID"],"is_corresponding":false,"raw_author_name":"N E Dungga","raw_affiliation_strings":["Department of Agronomy, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Agronomy, Faculty of Agriculture, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar, 90245, Indonesia","institution_ids":["https://openalex.org/I98301867"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5013633593"],"corresponding_institution_ids":["https://openalex.org/I98301867"],"apc_list":null,"apc_paid":null,"fwci":2.0514,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.8803617,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"157","issue":null,"first_page":"012044","last_page":"012044"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11578","display_name":"Plant Pathogenic Bacteria Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chrysanthemum-morifolium","display_name":"Chrysanthemum morifolium","score":0.9919999837875366},{"id":"https://openalex.org/keywords/agrobacterium-mediated-transformation","display_name":"Agrobacterium-mediated transformation","score":0.9340000152587891},{"id":"https://openalex.org/keywords/antibiotic-selection","display_name":"antibiotic selection","score":0.675000011920929},{"id":"https://openalex.org/keywords/kanamycin","display_name":"kanamycin","score":0.9539999961853027},{"id":"https://openalex.org/keywords/hygromycin","display_name":"hygromycin","score":1.0},{"id":"https://openalex.org/keywords/paromomycin","display_name":"paromomycin","score":0.9869999885559082},{"id":"https://openalex.org/keywords/callus-growth","display_name":"callus growth","score":0.8790000081062317},{"id":"https://openalex.org/keywords/leaf-explants","display_name":"leaf explants","score":0.7250000238418579},{"id":"https://openalex.org/keywords/disease-resistance","display_name":"disease resistance","score":0.6759999990463257},{"id":"https://openalex.org/keywords/co-culture","display_name":"co-culture","score":0.7009999752044678},{"id":"https://openalex.org/keywords/transformation-protocol","display_name":"transformation protocol","score":0.4860000014305115}],"concepts":[{"id":"https://openalex.org/C2778874994","wikidata":"https://www.wikidata.org/wiki/Q15530181","display_name":"Chrysanthemum morifolium","level":2,"score":0.9642334580421448},{"id":"https://openalex.org/C9549007","wikidata":"https://www.wikidata.org/wiki/Q1072909","display_name":"Callus","level":2,"score":0.8015817403793335},{"id":"https://openalex.org/C2779656422","wikidata":"https://www.wikidata.org/wiki/Q2700446","display_name":"Agrobacterium","level":4,"score":0.7556383609771729},{"id":"https://openalex.org/C204241405","wikidata":"https://www.wikidata.org/wiki/Q461499","display_name":"Transformation (genetics)","level":3,"score":0.720291018486023},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6202586889266968},{"id":"https://openalex.org/C81917197","wikidata":"https://www.wikidata.org/wiki/Q628760","display_name":"Selection (genetic algorithm)","level":2,"score":0.48609161376953125},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.47714564204216003},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.399597704410553},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.38829588890075684},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11046496033668518},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.09984704852104187},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07478198409080505},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1088/1755-1315/157/1/012044","doi":"https://doi.org/10.1088/1755-1315/157/1/012044","is_oa":true,"landing_page_url":"https://doi.org/10.1088/1755-1315/157/1/012044","pdf_url":"https://iopscience.iop.org/article/10.1088/1755-1315/157/1/012044/pdf","source":{"id":"https://openalex.org/S4210195883","display_name":"IOP Conference Series Earth and Environmental Science","issn_l":"1755-1315","issn":["1755-1315","1755-1307"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320083","host_organization_name":"IOP Publishing","host_organization_lineage":["https://openalex.org/P4310320083","https://openalex.org/P4310311669"],"host_organization_lineage_names":["IOP Publishing","Institute of Physics"],"type":"conference"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IOP Conference Series: Earth and Environmental Science","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1088/1755-1315/157/1/012044","doi":"https://doi.org/10.1088/1755-1315/157/1/012044","is_oa":true,"landing_page_url":"https://doi.org/10.1088/1755-1315/157/1/012044","pdf_url":"https://iopscience.iop.org/article/10.1088/1755-1315/157/1/012044/pdf","source":{"id":"https://openalex.org/S4210195883","display_name":"IOP Conference Series Earth and Environmental Science","issn_l":"1755-1315","issn":["1755-1315","1755-1307"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320083","host_organization_name":"IOP Publishing","host_organization_lineage":["https://openalex.org/P4310320083","https://openalex.org/P4310311669"],"host_organization_lineage_names":["IOP Publishing","Institute of Physics"],"type":"conference"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"IOP Conference Series: Earth and Environmental Science","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.6875,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2806478209.pdf","grobid_xml":"https://content.openalex.org/works/W2806478209.grobid-xml"},"referenced_works_count":13,"referenced_works":["https://openalex.org/W1543032633","https://openalex.org/W1964972616","https://openalex.org/W1973008533","https://openalex.org/W1996517771","https://openalex.org/W2011733259","https://openalex.org/W2043196539","https://openalex.org/W2052285585","https://openalex.org/W2065103871","https://openalex.org/W2071788546","https://openalex.org/W2085263778","https://openalex.org/W2091130986","https://openalex.org/W2108439094","https://openalex.org/W2142605443"],"related_works":["https://openalex.org/W2188578082","https://openalex.org/W2053809080","https://openalex.org/W2184352435","https://openalex.org/W2903851177","https://openalex.org/W2344215007","https://openalex.org/W2016523014","https://openalex.org/W283269213","https://openalex.org/W2287306097","https://openalex.org/W2385637679","https://openalex.org/W1983565901"],"abstract_inverted_index":{"Genetic":[0],"transformation":[1,32,218],"mediated":[2,47],"by":[3,48,57],"Agrobacterium":[4,49,223],"tumefaciens":[5,50],"requires":[6],"an":[7],"efficient":[8,232],"selection":[9,28,156,209],"method":[10],"for":[11,162],"successful":[12],"progress":[13],"of":[14,25,37,42,81,133,140,143,149,221,239],"transformation.":[15],"This":[16],"study":[17],"aims":[18],"to":[19,33,192,244],"determine":[20],"the":[21,40,208,236],"concentration":[22,220],"and":[23,27,87,98,109,168],"kind":[24],"antibiotics":[26],"agents":[29],"used":[30,206],"during":[31],"formulate":[34],"standard":[35],"protocol":[36],"chrysanthemum":[38,59],"in":[39,118,147],"process":[41],"propagating":[43],"disease":[44],"resistant":[45],"Chrysanthemum":[46],"EHA105":[51],"(pEKB-WD).":[52],"The":[53,152,216],"experiments":[54],"were":[55,135],"performed":[56],"planting":[58],"explants":[60],"leaf":[61,144],"cutting":[62],"(5":[63],"mm":[64],"diameter":[65],"on":[66],"NAA":[67],"medium":[68],"2":[69,74],"mg":[70,75,165,171,186],"L":[71,76,90,101,112,166,172,187],"-1":[72,77,91,102,113,167,173,188],"BAP":[73],")":[78],"with":[79,219,225],"addition":[80],"Kanamycin:":[82],"25,":[83,96,106],"50,":[84,107],"100,":[85],"150":[86],"200":[88],"(mg":[89,100,111],");":[92,103],"Hygromycin:":[93],"5,":[94],"10,":[95,105],"50":[97],"75":[99,108,131],"Paromomycin:":[104],"100":[110,170,185],").":[114],"Experiment":[115],"was":[116,126,189,242],"arranged":[117],"a":[119,159],"Completely":[120],"Randomized":[121],"Design":[122],"(CRD).":[123],"Each":[124],"treatment":[125],"repeated":[127],"five":[128],"times":[129],"thus":[130],"bottles":[132],"culture":[134],"used;":[136],"each":[137],"bottle":[138],"consists":[139],"5":[141],"pieces":[142],"cuttings,":[145],"resulted":[146],"total":[148],"375":[150],"pieces.":[151],"results":[153],"showed":[154],"that":[155,204],"agent":[157,210],"had":[158],"critical":[160],"value":[161],"Hygromycin":[163],"25":[164],"Kanamycin":[169,246],"which":[174],"can":[175,229],"make":[176],"explant":[177],"experienced":[178],"necrosis":[179],"better":[180],"than":[181,201],"Paromomycin.":[182],"Paromomycin":[183],"at":[184],"only":[190],"able":[191],"kill":[193],"explant\u2019s":[194],"periphery.":[195],"Remained":[196],"callus":[197],"stayed":[198],"fresh":[199],"more":[200,213,231],"50%":[202],"so":[203],"when":[205],"as":[207,247],"could":[211],"produce":[212,230],"escape":[214],"cell.":[215],"optimum":[217],"10%":[222],"(vol/vol)":[224],"30":[226],"minutes":[227],"co-cultivation":[228],"transformed":[233],"callus.":[234],"Considering":[235],"high":[237],"price":[238],"Hygromycin,":[240],"it":[241],"best":[243],"use":[245],"selective":[248],"agents.":[249]},"counts_by_year":[{"year":2022,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2018-06-13T00:00:00"}