{"id":"https://openalex.org/W2804842328","doi":"https://doi.org/10.1002/jssc.201701473","title":"Application of denaturing capillary electrophoresis for the detection of prognostic mutations in isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 genes in brain tumors","display_name":"Application of denaturing capillary electrophoresis for the detection of prognostic mutations in isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 genes in brain tumors","publication_year":2018,"publication_date":"2018-05-23","ids":{"openalex":"https://openalex.org/W2804842328","doi":"https://doi.org/10.1002/jssc.201701473","mag":"2804842328","pmid":"https://pubmed.ncbi.nlm.nih.gov/29791066"},"language":"en","primary_location":{"id":"doi:10.1002/jssc.201701473","doi":"https://doi.org/10.1002/jssc.201701473","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jssc.201701473","pdf_url":null,"source":{"id":"https://openalex.org/S45406943","display_name":"Journal of Separation Science","issn_l":"1615-9306","issn":["1615-9306","1615-9314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Separation Science","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5065070816","display_name":"Lucie Bene\u0161ov\u00e1","orcid":"https://orcid.org/0000-0001-7664-0520","observed_orcids":["https://orcid.org/0000-0001-7664-0520"]},"institutions":[{"id":"https://openalex.org/I4210127241","display_name":"Genomac","ror":"https://ror.org/02z71hx86","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210127241"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Lucie Benesova","raw_affiliation_strings":["Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210127241"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022284593","display_name":"Barbora Bel\u0161\u00e1nov\u00e1","orcid":"https://orcid.org/0009-0008-1586-0405","observed_orcids":["https://orcid.org/0009-0008-1586-0405"]},"institutions":[{"id":"https://openalex.org/I4210127241","display_name":"Genomac","ror":"https://ror.org/02z71hx86","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210127241"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Barbora Belsanova","raw_affiliation_strings":["Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210127241"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059948286","display_name":"Filip Kram\u00e1\u0159","orcid":"https://orcid.org/0009-0007-2452-588X","observed_orcids":["https://orcid.org/0009-0007-2452-588X","https://orcid.org/0000-0003-0833-2360"]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]},{"id":"https://openalex.org/I4210130875","display_name":"Military University Hospital Prague","ror":"https://ror.org/03a8sgj63","country_code":"CZ","type":"education","lineage":["https://openalex.org/I4210130875"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Filip Kramar","raw_affiliation_strings":["Department of Neurosurgery, First Faculty of Medicine, Military University Hospital and Charles University, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, First Faculty of Medicine, Military University Hospital and Charles University, Prague, Czech Republic","institution_ids":["https://openalex.org/I21250087","https://openalex.org/I4210130875"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033228752","display_name":"Tereza H\u00e1lkov\u00e1","orcid":"https://orcid.org/0009-0000-2985-7145","observed_orcids":["https://orcid.org/0009-0000-2985-7145","https://orcid.org/0000-0001-9320-4210"]},"institutions":[{"id":"https://openalex.org/I4210127241","display_name":"Genomac","ror":"https://ror.org/02z71hx86","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210127241"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Tereza Halkova","raw_affiliation_strings":["Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210127241"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090708085","display_name":"Vladim\u00edr Bene\u0161","orcid":"https://orcid.org/0000-0002-6889-857X","observed_orcids":["https://orcid.org/0000-0002-6889-857X"]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]},{"id":"https://openalex.org/I4210130875","display_name":"Military University Hospital Prague","ror":"https://ror.org/03a8sgj63","country_code":"CZ","type":"education","lineage":["https://openalex.org/I4210130875"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Vladimir Benes","raw_affiliation_strings":["Department of Neurosurgery, First Faculty of Medicine, Military University Hospital and Charles University, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Neurosurgery, First Faculty of Medicine, Military University Hospital and Charles University, Prague, Czech Republic","institution_ids":["https://openalex.org/I21250087","https://openalex.org/I4210130875"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5052949409","display_name":"Marek Min\u00e1rik","orcid":"https://orcid.org/0000-0002-0615-6116","observed_orcids":["https://orcid.org/0000-0002-0615-6116"]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]},{"id":"https://openalex.org/I4210127241","display_name":"Genomac","ror":"https://ror.org/02z71hx86","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210127241"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"Marek Minarik","raw_affiliation_strings":["Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic","Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic"],"raw_orcid":"https://orcid.org/0000-0002-0615-6116","affiliations":[{"raw_affiliation_string":"Center for Applied Genomics of Solid Tumors (CEGES), Genomac Research Institute, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210127241"]},{"raw_affiliation_string":"Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic","institution_ids":["https://openalex.org/I21250087"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5052949409"],"corresponding_institution_ids":["https://openalex.org/I21250087","https://openalex.org/I4210127241"],"apc_list":{"value":4030,"currency":"USD","value_usd":4030},"apc_paid":null,"fwci":0.1043,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.44923021,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"41","issue":"13","first_page":"2819","last_page":"2827"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.7310000061988831,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.7310000061988831,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.19130000472068787,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.015399999916553497,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/idh1","display_name":"IDH1","score":0.9459999799728394},{"id":"https://openalex.org/keywords/idh2","display_name":"IDH2","score":0.9959999918937683},{"id":"https://openalex.org/keywords/idh-mutation","display_name":"IDH mutation","score":0.7929999828338623},{"id":"https://openalex.org/keywords/prognostic-factors","display_name":"prognostic factors","score":0.7540000081062317},{"id":"https://openalex.org/keywords/brain-tumors","display_name":"brain tumors","score":0.7950000166893005},{"id":"https://openalex.org/keywords/glioma","display_name":"glioma","score":0.8539999723434448},{"id":"https://openalex.org/keywords/mutation-detection-assay","display_name":"mutation detection assay","score":0.9160000085830688},{"id":"https://openalex.org/keywords/multiplex-ligation-dependent-probe-amplification","display_name":"multiplex ligation-dependent probe amplification","score":0.8920000195503235},{"id":"https://openalex.org/keywords/assay-validation","display_name":"assay validation","score":0.597000002861023},{"id":"https://openalex.org/keywords/detection-sensitivity","display_name":"detection sensitivity","score":0.5529999732971191}],"concepts":[{"id":"https://openalex.org/C2777150147","wikidata":"https://www.wikidata.org/wiki/Q261968","display_name":"Isocitrate dehydrogenase","level":3,"score":0.9721212387084961},{"id":"https://openalex.org/C2900643","wikidata":"https://www.wikidata.org/wiki/Q747468","display_name":"Capillary electrophoresis","level":2,"score":0.7184610962867737},{"id":"https://openalex.org/C127848430","wikidata":"https://www.wikidata.org/wiki/Q18027543","display_name":"IDH1","level":4,"score":0.5110934376716614},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.48066437244415283},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4742649793624878},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.46066537499427795},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.4352220892906189},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.42951256036758423},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.375146746635437},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36997973918914795},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3038349151611328},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2927277684211731}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001932","descriptor_name":"Brain Neoplasms","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":false},{"descriptor_ui":"D001932","descriptor_name":"Brain Neoplasms","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001932","descriptor_name":"Brain Neoplasms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007521","descriptor_name":"Isocitrate Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019075","descriptor_name":"Electrophoresis, Capillary","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/jssc.201701473","doi":"https://doi.org/10.1002/jssc.201701473","is_oa":false,"landing_page_url":"https://doi.org/10.1002/jssc.201701473","pdf_url":null,"source":{"id":"https://openalex.org/S45406943","display_name":"Journal of Separation Science","issn_l":"1615-9306","issn":["1615-9306","1615-9314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Separation Science","raw_type":"journal-article"},{"id":"pmid:29791066","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29791066","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of separation science","raw_type":"Evaluation Study"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320328734","display_name":"Ministerstvo Obrany \u010cesk\u00e9 Republiky","ror":"https://ror.org/00r7raa16"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W1574091519","https://openalex.org/W1757407923","https://openalex.org/W1968391269","https://openalex.org/W1968916503","https://openalex.org/W1980811022","https://openalex.org/W1981242670","https://openalex.org/W2003059099","https://openalex.org/W2024605144","https://openalex.org/W2025183726","https://openalex.org/W2028508804","https://openalex.org/W2040827479","https://openalex.org/W2048661839","https://openalex.org/W2064809931","https://openalex.org/W2065185874","https://openalex.org/W2069059773","https://openalex.org/W2074803464","https://openalex.org/W2092609843","https://openalex.org/W2111988427","https://openalex.org/W2112495947","https://openalex.org/W2118151336","https://openalex.org/W2123299008","https://openalex.org/W2126817554","https://openalex.org/W2127096071","https://openalex.org/W2137881268","https://openalex.org/W2160300695","https://openalex.org/W2187257739","https://openalex.org/W2329357574","https://openalex.org/W2395251640","https://openalex.org/W2472638159","https://openalex.org/W2606116537","https://openalex.org/W2621915991"],"related_works":["https://openalex.org/W2800268572","https://openalex.org/W1911924650","https://openalex.org/W7169852216","https://openalex.org/W2567150820","https://openalex.org/W2912969415","https://openalex.org/W3182108023","https://openalex.org/W1589188602","https://openalex.org/W2291733974","https://openalex.org/W2916392296","https://openalex.org/W4386022989"],"abstract_inverted_index":{"Malignant":[0],"transformation":[1],"in":[2,10,25,40,50,123],"gliomas":[3],"is":[4],"frequently":[5],"supplemented":[6],"by":[7,145,150],"somatic":[8],"mutations":[9,24],"isocitrate":[11,15,56,108],"dehydrogenase":[12,16,57,109],"1":[13,58,110],"and":[14,33,59,91,102,106,111,197],"2":[17,60,112],"genes.":[18],"It":[19],"has":[20],"recently":[21],"emerged":[22],"that":[23],"these":[26,63],"genes":[27],"are":[28,47],"associated":[29],"with":[30,71],"prolonged":[31],"survival":[32],"should":[34],"be":[35,95],"used":[36],"as":[37,68],"prognostic":[38],"factor":[39],"management":[41],"of":[42,55,85,104,125,136,164,170,184,190],"brain":[43,166],"cancer":[44],"patients.":[45],"There":[46],"several":[48],"approaches":[49],"use":[51],"for":[52],"the":[53,126,156],"detection":[54,114,121,154],"mutations;":[61],"however,":[62],"often":[64],"exhibit":[65],"shortcomings":[66],"such":[67],"convoluted":[69],"protocols":[70],"long":[72],"processing":[73],"time,":[74],"complex":[75],"(and":[76],"costly)":[77],"dedicated":[78],"fluorescent":[79],"probes,":[80],"and/or":[81],"demand":[82],"on":[83,161],"amounts":[84],"input":[86,183],"DNA.":[87],"Therefore,":[88],"a":[89,134,151,162],"simple":[90,105],"rapid":[92],"method":[93,142],"would":[94],"highly":[96],"desired.":[97],"Here,":[98],"we":[99],"present":[100],"development":[101],"validation":[103],"reliable":[107],"mutation":[113],"assay":[115,179],"using":[116],"denaturing":[117,176],"capillary":[118,177],"electrophoresis.":[119],"The":[120,141,168],"sensitivity":[122],"terms":[124],"limiting":[127],"mutated":[128],"allele":[129],"fraction":[130],"detectable":[131],"estimated":[132],"from":[133],"series":[135],"dilution":[137],"runs":[138],"was":[139,143,173],"2.9%.":[140],"validated":[144],"comparing":[146],"to":[147],"results":[148],"obtained":[149],"widely":[152],"accepted":[153],"technique,":[155],"multiplex":[157],"ligation-dependent":[158],"probe":[159],"amplification,":[160],"set":[163],"85":[165],"tumors.":[167],"concordance":[169],"both":[171],"methods":[172],"100%,":[174],"but":[175],"electrophoresis":[178],"required":[180],"fivefold":[181],"lower":[182],"DNA":[185,191],"(1":[186],"versus":[187],"5":[188],"\u03bcL":[189],"at":[192],"concentrations":[193],"typically":[194],"between":[195],"10":[196],"30":[198],"ng/\u03bcL).":[199]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2019,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2018-06-01T00:00:00"}
