{"id":"https://openalex.org/W2803828233","doi":"https://doi.org/10.1002/cpmb.52","title":"Pooled Lentiviral\u2010Delivery Genetic Screens","display_name":"Pooled Lentiviral\u2010Delivery Genetic Screens","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2803828233","doi":"https://doi.org/10.1002/cpmb.52","mag":"2803828233","pmid":"https://pubmed.ncbi.nlm.nih.gov/29337374"},"language":"en","primary_location":{"id":"doi:10.1002/cpmb.52","doi":"https://doi.org/10.1002/cpmb.52","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cpmb.52","pdf_url":null,"source":{"id":"https://openalex.org/S2755386604","display_name":"Current Protocols in Molecular Biology","issn_l":"1934-3639","issn":["1934-3639","1934-3647"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Molecular Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000745052","display_name":"Federica Piccioni","orcid":"https://orcid.org/0000-0003-1210-3210","observed_orcids":["https://orcid.org/0000-0003-1210-3210"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Federica Piccioni","raw_affiliation_strings":["Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I107606265"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041456482","display_name":"Scott T. Younger","orcid":"https://orcid.org/0000-0002-6237-445X","observed_orcids":["https://orcid.org/0000-0002-6237-445X"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott T. Younger","raw_affiliation_strings":["Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I107606265"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5086771961","display_name":"David E. Root","orcid":"https://orcid.org/0000-0001-5122-861X","observed_orcids":["https://orcid.org/0000-0001-5122-861X","https://orcid.org/0000-0003-0096-4830"]},"institutions":[{"id":"https://openalex.org/I107606265","display_name":"Broad Institute","ror":"https://ror.org/05a0ya142","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I107606265"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"David E. Root","raw_affiliation_strings":["Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Genetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I107606265"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I107606265"],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":1.9627,"has_fulltext":false,"cited_by_count":29,"citation_normalized_percentile":{"value":0.8600917,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":98},"biblio":{"volume":"121","issue":"1","first_page":"32.1.1","last_page":"32.1.21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.5985999703407288,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.5985999703407288,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.34619998931884766,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.013000000268220901,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.7490000128746033},{"id":"https://openalex.org/keywords/viral-transduction","display_name":"viral transduction","score":0.5649999976158142},{"id":"https://openalex.org/keywords/rna-interference","display_name":"RNA interference","score":0.8349999785423279},{"id":"https://openalex.org/keywords/ectopic-expression","display_name":"ectopic expression","score":0.9169999957084656},{"id":"https://openalex.org/keywords/barcode-sequencing","display_name":"barcode sequencing","score":0.5270000100135803},{"id":"https://openalex.org/keywords/gene-function","display_name":"gene function","score":0.4959999918937683}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7468650341033936},{"id":"https://openalex.org/C207177822","wikidata":"https://www.wikidata.org/wiki/Q5050812","display_name":"Genetic screen","level":4,"score":0.7351706624031067},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7273521423339844},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6308281421661377},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.628673255443573},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.5909797549247742},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5522350668907166},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.520512044429779},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.5153629183769226},{"id":"https://openalex.org/C189819185","wikidata":"https://www.wikidata.org/wiki/Q384283","display_name":"Subgenomic mRNA","level":4,"score":0.44621074199676514},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4321153461933136},{"id":"https://openalex.org/C15152581","wikidata":"https://www.wikidata.org/wiki/Q7833966","display_name":"Transduction (biophysics)","level":2,"score":0.4273435175418854},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.36018988490104675},{"id":"https://openalex.org/C144024400","wikidata":"https://www.wikidata.org/wiki/Q21201","display_name":"Sociology","level":0,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0},{"id":"https://openalex.org/C149923435","wikidata":"https://www.wikidata.org/wiki/Q37732","display_name":"Demography","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000066630","descriptor_name":"Ectopic Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D034622","descriptor_name":"RNA Interference","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055786","descriptor_name":"Gene Knockout Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/cpmb.52","doi":"https://doi.org/10.1002/cpmb.52","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cpmb.52","pdf_url":null,"source":{"id":"https://openalex.org/S2755386604","display_name":"Current Protocols in Molecular Biology","issn_l":"1934-3639","issn":["1934-3639","1934-3647"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","poland-100"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Molecular Biology","raw_type":"journal-article"},{"id":"pmid:29337374","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29337374","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in molecular biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":9,"referenced_works":["https://openalex.org/W1965440903","https://openalex.org/W1986028255","https://openalex.org/W2100122648","https://openalex.org/W2115718128","https://openalex.org/W2131539955","https://openalex.org/W2174849886","https://openalex.org/W2252568502","https://openalex.org/W2412521064","https://openalex.org/W2601885162"],"related_works":["https://openalex.org/W4206247338","https://openalex.org/W2896929167","https://openalex.org/W4385718412","https://openalex.org/W3189387552","https://openalex.org/W2416331553","https://openalex.org/W2460103355","https://openalex.org/W2561754210","https://openalex.org/W6982342174","https://openalex.org/W1982873976","https://openalex.org/W2584408647"],"abstract_inverted_index":{"Pooled":[0,16],"cell-based":[1],"screens":[2,17,113],"of":[3,13,41,57,63,97,100,107,134],"mammalian":[4],"genetic":[5,19,82],"perturbations":[6,20,43,83],"enable":[7],"systematic":[8],"large-scale,":[9],"even":[10],"genome-scale,":[11],"evaluation":[12],"gene":[14,88,91],"function.":[15],"introduce":[18],"into":[21,33],"a":[22,36,140],"cell":[23,31,108],"population":[24],"through":[25],"viral":[26],"transduction":[27],"such":[28,112],"that":[29,59],"each":[30],"integrates":[32],"its":[34],"DNA":[35],"single":[37],"or":[38,76,104],"small":[39],"number":[40],"library":[42,101],"with":[44],"barcodes":[45,67],"identifying":[46],"the":[47,55,61,66,69,78,98,105,127,135],"perturbations.":[48],"One":[49],"then":[50],"selects":[51],"and":[52,93,118,130],"physically":[53],"isolates":[54],"subset":[56],"cells":[58,71],"exhibit":[60],"phenotype":[62],"interest.":[64],"Sequencing":[65],"in":[68],"hit":[70,79],"reveals":[72],"which":[73],"genes":[74],"favored":[75],"inhibited":[77],"phenotype.":[80],"Various":[81],"are":[84],"possible,":[85],"including":[86],"CRISPR":[87],"knockout,":[89],"ectopic":[90],"expression,":[92],"RNA":[94],"interference.":[95],"Regardless":[96],"type":[99,106],"being":[102,110],"screened":[103],"model":[109],"tested,":[111],"involve":[114],"many":[115],"common":[116],"steps":[117],"procedures.":[119],"This":[120],"unit":[121],"describes":[122],"detailed":[123],"experimental":[124],"protocols":[125],"for":[126],"key":[128,136],"steps,":[129],"also":[131],"highlights":[132],"some":[133],"factors":[137],"to":[138],"achieving":[139],"well-powered,":[141],"reproducible":[142],"screen":[143],"result.":[144],"\u00a9":[145],"2018":[146],"by":[147],"John":[148],"Wiley":[149],"&":[150],"Sons,":[151],"Inc.":[152]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":4},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":5}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2018-06-01T00:00:00"}