{"id":"https://openalex.org/W2803273233","doi":"https://doi.org/10.1039/c8cs00085a","title":"Engineering enzyme microenvironments for enhanced biocatalysis","display_name":"Engineering enzyme microenvironments for enhanced biocatalysis","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2803273233","doi":"https://doi.org/10.1039/c8cs00085a","mag":"2803273233","pmid":"https://pubmed.ncbi.nlm.nih.gov/29796541"},"language":"en","primary_location":{"id":"doi:10.1039/c8cs00085a","doi":"https://doi.org/10.1039/c8cs00085a","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c8cs00085a","pdf_url":null,"source":{"id":"https://openalex.org/S316438","display_name":"Chemical Society Reviews","issn_l":"0306-0012","issn":["0306-0012","1460-4744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical Society Reviews","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071708056","display_name":"Louis Lancaster","orcid":"https://orcid.org/0000-0002-1272-6488","observed_orcids":["https://orcid.org/0000-0002-1272-6488"]},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Louis Lancaster","raw_affiliation_strings":["Department of Chemical and Environmental Engineering","Riverside","USA","University of California, Riverside","Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA 92521, USA"],"raw_orcid":"https://orcid.org/0000-0002-1272-6488","affiliations":[{"raw_affiliation_string":"Department of Chemical and Environmental Engineering","institution_ids":[]},{"raw_affiliation_string":"Riverside","institution_ids":[]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"University of California, Riverside","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073014396","display_name":"Walaa Abdallah","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Walaa Abdallah","raw_affiliation_strings":["Columbia University","Department of Chemical Engineering","New York","USA","Department of Chemical Engineering, Columbia University, New York, New York 10027, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Columbia University","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Chemical Engineering","institution_ids":[]},{"raw_affiliation_string":"New York","institution_ids":[]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063109716","display_name":"Scott A. Banta","orcid":"https://orcid.org/0000-0001-7885-0150","observed_orcids":["https://orcid.org/0000-0001-7885-0150"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Scott Banta","raw_affiliation_strings":["Columbia University","Department of Chemical Engineering","New York","USA","Department of Chemical Engineering, Columbia University, New York, New York 10027, USA"],"raw_orcid":"https://orcid.org/0000-0001-7885-0150","affiliations":[{"raw_affiliation_string":"Columbia University","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Chemical Engineering","institution_ids":[]},{"raw_affiliation_string":"New York","institution_ids":[]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"Department of Chemical Engineering, Columbia University, New York, New York 10027, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051470799","display_name":"Ian R. Wheeldon","orcid":"https://orcid.org/0000-0002-3492-7539","observed_orcids":["https://orcid.org/0000-0002-3492-7539"]},"institutions":[{"id":"https://openalex.org/I103635307","display_name":"University of California, Riverside","ror":"https://ror.org/03nawhv43","country_code":"US","type":"education","lineage":["https://openalex.org/I103635307"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ian Wheeldon","raw_affiliation_strings":["Department of Chemical and Environmental Engineering","Riverside","USA","University of California, Riverside","Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA 92521, USA"],"raw_orcid":"https://orcid.org/0000-0002-3492-7539","affiliations":[{"raw_affiliation_string":"Department of Chemical and Environmental Engineering","institution_ids":[]},{"raw_affiliation_string":"Riverside","institution_ids":[]},{"raw_affiliation_string":"USA","institution_ids":[]},{"raw_affiliation_string":"University of California, Riverside","institution_ids":["https://openalex.org/I103635307"]},{"raw_affiliation_string":"Department of Chemical and Environmental Engineering, University of California, Riverside, Riverside, CA 92521, USA","institution_ids":["https://openalex.org/I103635307"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5051470799","https://openalex.org/A5063109716"],"corresponding_institution_ids":["https://openalex.org/I103635307","https://openalex.org/I78577930"],"apc_list":null,"apc_paid":null,"fwci":1.6429,"has_fulltext":false,"cited_by_count":184,"citation_normalized_percentile":{"value":0.85611363,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"47","issue":"14","first_page":"5177","last_page":"5186"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-engineering","display_name":"protein engineering","score":0.8240000009536743},{"id":"https://openalex.org/keywords/biocatalysis","display_name":"biocatalysis","score":0.8930000066757202},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7369999885559082},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.9629999995231628},{"id":"https://openalex.org/keywords/ph-tolerance","display_name":"pH tolerance","score":0.7070000171661377}],"concepts":[{"id":"https://openalex.org/C191503008","wikidata":"https://www.wikidata.org/wiki/Q16007026","display_name":"Biocatalysis","level":4,"score":0.8908276557922363},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5256938934326172},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.5163692235946655},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.4917481243610382},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4108927249908447},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2618686556816101},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.23087862133979797},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.1571362316608429},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.15344616770744324},{"id":"https://openalex.org/C65024703","wikidata":"https://www.wikidata.org/wiki/Q373406","display_name":"Reaction mechanism","level":3,"score":0.06449893116950989}],"mesh":[{"descriptor_ui":"D000426","descriptor_name":"Alcohol Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004798","descriptor_name":"Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006735","descriptor_name":"Horseradish Peroxidase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1039/c8cs00085a","doi":"https://doi.org/10.1039/c8cs00085a","is_oa":false,"landing_page_url":"https://doi.org/10.1039/c8cs00085a","pdf_url":null,"source":{"id":"https://openalex.org/S316438","display_name":"Chemical Society Reviews","issn_l":"0306-0012","issn":["0306-0012","1460-4744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-3","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320556","host_organization_name":"Royal Society of Chemistry","host_organization_lineage":["https://openalex.org/P4310320556"],"host_organization_lineage_names":["Royal Society of Chemistry"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical Society Reviews","raw_type":"journal-article"},{"id":"pmid:29796541","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29796541","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chemical Society reviews","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5783512236","display_name":null,"funder_award_id":"MURI W911NF1410263","funder_id":"https://openalex.org/F4320338281","funder_display_name":"Army Research Office"}],"funders":[{"id":"https://openalex.org/F4320333591","display_name":"Multidisciplinary University Research Initiative","ror":null},{"id":"https://openalex.org/F4320338281","display_name":"Army Research Office","ror":"https://ror.org/05epdh915"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":55,"referenced_works":["https://openalex.org/W1965560011","https://openalex.org/W1969941075","https://openalex.org/W1973254329","https://openalex.org/W1975977260","https://openalex.org/W1976236461","https://openalex.org/W1983164158","https://openalex.org/W1984180361","https://openalex.org/W1989548144","https://openalex.org/W1990533573","https://openalex.org/W2001290593","https://openalex.org/W2003504234","https://openalex.org/W2015115860","https://openalex.org/W2019403558","https://openalex.org/W2031447561","https://openalex.org/W2041710436","https://openalex.org/W2042994271","https://openalex.org/W2048464086","https://openalex.org/W2064030623","https://openalex.org/W2073016734","https://openalex.org/W2087366584","https://openalex.org/W2095753590","https://openalex.org/W2103385859","https://openalex.org/W2116499195","https://openalex.org/W2120549005","https://openalex.org/W2121725915","https://openalex.org/W2133265349","https://openalex.org/W2144794655","https://openalex.org/W2151052950","https://openalex.org/W2163986426","https://openalex.org/W2164697359","https://openalex.org/W2166587421","https://openalex.org/W2206621017","https://openalex.org/W2249095822","https://openalex.org/W2283818235","https://openalex.org/W2301507491","https://openalex.org/W2303875806","https://openalex.org/W2312277996","https://openalex.org/W2317402410","https://openalex.org/W2320321428","https://openalex.org/W2322844285","https://openalex.org/W2331719657","https://openalex.org/W2365746119","https://openalex.org/W2406900093","https://openalex.org/W2510669564","https://openalex.org/W2566722304","https://openalex.org/W2586562809","https://openalex.org/W2587180351","https://openalex.org/W2738129447","https://openalex.org/W2754808391","https://openalex.org/W2770827559","https://openalex.org/W2771343295","https://openalex.org/W2776200197","https://openalex.org/W2782486101","https://openalex.org/W4210980745","https://openalex.org/W4250838544"],"related_works":["https://openalex.org/W4386179313","https://openalex.org/W4283258939","https://openalex.org/W3023267415","https://openalex.org/W3012429034","https://openalex.org/W3169299531","https://openalex.org/W2055212608","https://openalex.org/W4400845423","https://openalex.org/W2790653442","https://openalex.org/W2915091113","https://openalex.org/W2021259617"],"abstract_inverted_index":{"Protein":[0],"engineering":[1,19,35,55,102,112],"provides":[2],"a":[3],"means":[4],"to":[5,10,24,46,86,119],"alter":[6],"protein":[7,101],"structure":[8],"leading":[9],"new":[11,98,121],"functions.":[12,126],"Much":[13],"work":[14],"has":[15],"focused":[16],"on":[17],"the":[18,64],"of":[20,38,66,92],"enzyme":[21,68,88],"active":[22,110],"sites":[23],"enhance":[25],"catalytic":[26],"activity,":[27],"however":[28],"there":[29],"is":[30],"an":[31,56],"increasing":[32],"trend":[33],"towards":[34],"other":[36],"aspects":[37],"biocatalysts":[39],"as":[40],"these":[41,87],"efforts":[42],"can":[43,114],"also":[44,94],"lead":[45],"useful":[47],"improvements.":[48],"This":[49],"tutorial":[50],"discusses":[51],"recent":[52],"advances":[53],"in":[54,75],"enzyme's":[57],"local":[58],"chemical":[59],"and":[60,73,100,106,113],"physical":[61],"environment,":[62],"with":[63,124],"goal":[65],"enhancing":[67],"reaction":[69],"kinetics,":[70],"substrate":[71],"selectivity,":[72],"activity":[74,93],"harsh":[76],"conditions":[77],"(e.g.,":[78],"low":[79],"or":[80],"high":[81],"pH).":[82],"By":[83],"introducing":[84],"stimuli-responsiveness":[85],"modifications,":[89],"dynamic":[90],"control":[91],"becomes":[95],"possible.":[96],"These":[97],"biomolecular":[99],"techniques":[103],"are":[104],"separate":[105],"independent":[107],"from":[108],"traditional":[109],"site":[111],"therefore":[115],"be":[116],"applied":[117],"synergistically":[118],"create":[120],"biocatalyst":[122],"technologies":[123],"novel":[125]},"counts_by_year":[{"year":2026,"cited_by_count":26},{"year":2025,"cited_by_count":17},{"year":2024,"cited_by_count":22},{"year":2023,"cited_by_count":22},{"year":2022,"cited_by_count":30},{"year":2021,"cited_by_count":24},{"year":2020,"cited_by_count":24},{"year":2019,"cited_by_count":15},{"year":2018,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-06-01T00:00:00"}