{"id":"https://openalex.org/W2803075469","doi":"https://doi.org/10.1094/asbcj-2016-2300-01","title":"AN-PEP, Proline-Specific Endopeptidase, Degrades All Known Immunostimulatory Gluten Peptides in Beer Made from Barley Malt","display_name":"AN-PEP, Proline-Specific Endopeptidase, Degrades All Known Immunostimulatory Gluten Peptides in Beer Made from Barley Malt","publication_year":2016,"publication_date":"2016-04-01","ids":{"openalex":"https://openalex.org/W2803075469","doi":"https://doi.org/10.1094/asbcj-2016-2300-01","mag":"2803075469"},"language":"en","primary_location":{"id":"doi:10.1094/asbcj-2016-2300-01","doi":"https://doi.org/10.1094/asbcj-2016-2300-01","is_oa":false,"landing_page_url":"https://doi.org/10.1094/asbcj-2016-2300-01","pdf_url":null,"source":{"id":"https://openalex.org/S192057034","display_name":"Journal of the American Society of Brewing Chemists","issn_l":"0361-0470","issn":["0361-0470","1943-7854"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Brewing Chemists","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5061855942","display_name":"Michiel Akeroyd","orcid":"https://orcid.org/0000-0002-3526-5821","observed_orcids":["https://orcid.org/0000-0002-3526-5821"]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":true,"raw_author_name":"Michiel Akeroyd","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012399122","display_name":"Sylvie. Van Zandycke","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sylvie van Zandycke","raw_affiliation_strings":["DSM Food Specialties, South Bend, IN, U.S.A"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Food Specialties, South Bend, IN, U.S.A","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085834558","display_name":"Joost den Hartog","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Joost den Hartog","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080655512","display_name":"Joz\u00e9 Mutsaers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Joz\u00e9 Mutsaers","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073362659","display_name":"Luppo Edens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Luppo Edens","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091438247","display_name":"Marco A. van den Berg","orcid":"https://orcid.org/0000-0003-2770-9944","observed_orcids":["https://orcid.org/0000-0003-2770-9944"]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Marco van den Berg","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005075316","display_name":"Chantal Christis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I127558574","display_name":"DSM (Netherlands)","ror":"https://ror.org/00k01kt20","country_code":"NL","type":"company","lineage":["https://openalex.org/I127558574"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Chantal Christis","raw_affiliation_strings":["DSM Biotechnology Center, Delft, The Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"DSM Biotechnology Center, Delft, The Netherlands","institution_ids":["https://openalex.org/I127558574"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5061855942"],"corresponding_institution_ids":["https://openalex.org/I127558574"],"apc_list":null,"apc_paid":null,"fwci":3.0221,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.91401869,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"74","issue":"2","first_page":"91","last_page":"99"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11348","display_name":"Celiac Disease Research and Management","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2715","display_name":"Gastroenterology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11348","display_name":"Celiac Disease Research and Management","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2715","display_name":"Gastroenterology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10411","display_name":"Proteins in Food Systems","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11561","display_name":"Protein Hydrolysis and Bioactive Peptides","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/1106","display_name":"Food Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/gluten-degradation","display_name":"gluten degradation","score":0.6600000262260437},{"id":"https://openalex.org/keywords/beer-haze","display_name":"beer haze","score":0.7160000205039978},{"id":"https://openalex.org/keywords/celiac-disease","display_name":"celiac disease","score":0.6899999976158142},{"id":"https://openalex.org/keywords/proline-rich-peptides","display_name":"proline-rich peptides","score":0.6549999713897705},{"id":"https://openalex.org/keywords/brewing-enzymes","display_name":"brewing enzymes","score":0.3240000009536743}],"concepts":[{"id":"https://openalex.org/C2776336767","wikidata":"https://www.wikidata.org/wiki/Q188251","display_name":"Gluten","level":2,"score":0.8905227780342102},{"id":"https://openalex.org/C39699697","wikidata":"https://www.wikidata.org/wiki/Q869095","display_name":"Brewing","level":3,"score":0.8587856292724609},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6788968443870544},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.6054263710975647},{"id":"https://openalex.org/C195616568","wikidata":"https://www.wikidata.org/wiki/Q128711","display_name":"Epitope","level":3,"score":0.5681774020195007},{"id":"https://openalex.org/C2779815552","wikidata":"https://www.wikidata.org/wiki/Q484583","display_name":"Proline","level":3,"score":0.550049901008606},{"id":"https://openalex.org/C2780985968","wikidata":"https://www.wikidata.org/wiki/Q1276781","display_name":"Mashing","level":2,"score":0.5260405540466309},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.5009219646453857},{"id":"https://openalex.org/C3019118998","wikidata":"https://www.wikidata.org/wiki/Q2632776","display_name":"Gluten free","level":3,"score":0.4465672969818115},{"id":"https://openalex.org/C2776775109","wikidata":"https://www.wikidata.org/wiki/Q993804","display_name":"Prolyl endopeptidase","level":3,"score":0.43801575899124146},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3981020450592041},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.3446784019470215},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2207868993282318},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2117706537246704},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.18786346912384033},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.11728432774543762},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1094/asbcj-2016-2300-01","doi":"https://doi.org/10.1094/asbcj-2016-2300-01","is_oa":false,"landing_page_url":"https://doi.org/10.1094/asbcj-2016-2300-01","pdf_url":null,"source":{"id":"https://openalex.org/S192057034","display_name":"Journal of the American Society of Brewing Chemists","issn_l":"0361-0470","issn":["0361-0470","1943-7854"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Brewing Chemists","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":74,"referenced_works":["https://openalex.org/W1505668954","https://openalex.org/W1522850665","https://openalex.org/W1585085258","https://openalex.org/W1900239629","https://openalex.org/W1902709718","https://openalex.org/W1966082986","https://openalex.org/W1967954749","https://openalex.org/W1968933996","https://openalex.org/W1972376000","https://openalex.org/W1978413893","https://openalex.org/W1980517296","https://openalex.org/W1983678635","https://openalex.org/W1983905576","https://openalex.org/W1984970449","https://openalex.org/W2002382362","https://openalex.org/W2005127740","https://openalex.org/W2008829781","https://openalex.org/W2009820702","https://openalex.org/W2011150002","https://openalex.org/W2014088357","https://openalex.org/W2019578712","https://openalex.org/W2024070220","https://openalex.org/W2024216641","https://openalex.org/W2030810018","https://openalex.org/W2038814063","https://openalex.org/W2040741541","https://openalex.org/W2047250985","https://openalex.org/W2052763389","https://openalex.org/W2053291064","https://openalex.org/W2057509300","https://openalex.org/W2058760025","https://openalex.org/W2064283406","https://openalex.org/W2064565544","https://openalex.org/W2064574008","https://openalex.org/W2070095525","https://openalex.org/W2072735598","https://openalex.org/W2073471201","https://openalex.org/W2075259020","https://openalex.org/W2076063685","https://openalex.org/W2076389815","https://openalex.org/W2076637305","https://openalex.org/W2078462317","https://openalex.org/W2078759091","https://openalex.org/W2081437475","https://openalex.org/W2082220059","https://openalex.org/W2083562009","https://openalex.org/W2087682176","https://openalex.org/W2089068068","https://openalex.org/W2096941822","https://openalex.org/W2100748913","https://openalex.org/W2102760911","https://openalex.org/W2107555364","https://openalex.org/W2109997679","https://openalex.org/W2113819336","https://openalex.org/W2119520032","https://openalex.org/W2121047105","https://openalex.org/W2124247925","https://openalex.org/W2128310785","https://openalex.org/W2128649796","https://openalex.org/W2133942458","https://openalex.org/W2138732864","https://openalex.org/W2139127272","https://openalex.org/W2141820146","https://openalex.org/W2154128794","https://openalex.org/W2156369485","https://openalex.org/W2158758405","https://openalex.org/W2162143225","https://openalex.org/W2164137002","https://openalex.org/W2314943030","https://openalex.org/W2319702923","https://openalex.org/W2332518103","https://openalex.org/W2410393672","https://openalex.org/W2799946726","https://openalex.org/W4313333826"],"related_works":["https://openalex.org/W2783196317","https://openalex.org/W2264879409","https://openalex.org/W2766180443","https://openalex.org/W2948304478","https://openalex.org/W2410393672","https://openalex.org/W2991910594","https://openalex.org/W2603913820","https://openalex.org/W4398233342","https://openalex.org/W2332518103","https://openalex.org/W3138015584"],"abstract_inverted_index":{"The":[0,72],"haze-forming":[1,65],"peptides":[2,66],"and":[3,18,67,164],"proteins":[4,68],"present":[5,32],"in":[6,33,55,78,96,129,142,195],"beer":[7,46,70,207],"mainly":[8],"originate":[9],"from":[10,201],"the":[11,34,52,110,126,154,159,174,211],"grains":[12],"used.":[13],"Such":[14],"grains,":[15],"typically":[16],"barley":[17],"wheat,":[19],"also":[20],"incorporate":[21],"gluten.":[22],"Although":[23],"partially":[24],"hydrolyzed":[25],"during":[26,69,178],"brewing,":[27],"glutencontaining":[28],"protein":[29],"material":[30],"remains":[31],"final":[35],"beers.":[36],"Glutensensitive":[37],"people":[38],"might":[39],"therefore":[40],"not":[41,165],"be":[42],"able":[43,187],"to":[44,61,124,188],"consume":[45],"without":[47],"an":[48],"adverse":[49],"response.":[50],"AN-PEP,":[51,100],"proline-specific":[53],"peptidase":[54],"Brewers":[56],"Clarex,":[57],"is":[58,162,186],"commercially":[59],"applied":[60],"selectively":[62],"degrade":[63,189],"proline-rich,":[64],"fermentation.":[71],"unusually":[73],"high":[74],"percentage":[75],"of":[76,150,173,176,214,217],"proline":[77],"known":[79,191],"celiac-related":[80],"gluten":[81,101,151,177,193,221],"epitopes":[82,194],"suggests":[83],"that":[84,95,144,184,199],"they":[85],"form":[86],"ideal":[87],"substrates":[88],"for":[89],"AN-PEP":[90,185,209],"as":[91,205],"well.":[92],"We":[93,116,197],"confirmed":[94],"beers":[97,130,143],"prepared":[98],"with":[99],"levels":[102],"were":[103],"well":[104],"below":[105],"20":[106,148],"mg/kg":[107,149],"based":[108,152],"on":[109,153],"established":[111],"competitive":[112],"R5":[113],"ELISA":[114],"method.":[115],"used":[117],"a":[118,133,169,206],"sensitive":[119],"LC-MS/MS":[120],"method":[121,161],"developed":[122],"in-house":[123],"identify":[125],"peptides.":[127],"Only":[128],"brewed":[131],"through":[132],"conventional":[134],"process":[135],"could":[136],"we":[137],"find":[138],"epitope-containing":[139],"peptides,":[140],"even":[141,157],"contained":[145],"less":[146],"than":[147],"ELISA.":[155],"So":[156],"though":[158],"MS":[160],"qualitative":[163],"quantitative,":[166],"it":[167],"provided":[168],"more":[170],"detailed":[171],"view":[172],"fate":[175],"brewing.":[179],"Specifically,":[180],"these":[181],"data":[182],"indicate":[183],"all":[190,218],"immunogenic":[192],"beer.":[196],"conclude":[198],"apart":[200],"its":[202],"current":[203],"application":[204],"stabilizer,":[208],"has":[210],"additional":[212],"benefit":[213],"exhaustive":[215],"degradation":[216],"potentially":[219],"problematic":[220],"epitopes.":[222]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":4}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2018-05-17T00:00:00"}
