{"id":"https://openalex.org/W2803019401","doi":"https://doi.org/10.1002/cpsc.50","title":"CRISPR/Cas9\u2010based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells","display_name":"CRISPR/Cas9\u2010based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells","publication_year":2018,"publication_date":"2018-04-26","ids":{"openalex":"https://openalex.org/W2803019401","doi":"https://doi.org/10.1002/cpsc.50","mag":"2803019401","pmid":"https://pubmed.ncbi.nlm.nih.gov/30040245","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/6060633"},"language":"en","primary_location":{"id":"doi:10.1002/cpsc.50","doi":"https://doi.org/10.1002/cpsc.50","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cpsc.50","pdf_url":null,"source":{"id":"https://openalex.org/S2764651103","display_name":"Current Protocols in Stem Cell Biology","issn_l":"1938-8969","issn":["1938-8969","1941-7322"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1","poland-70"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Stem Cell Biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6060633","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081692636","display_name":"Navin R. Gupta","orcid":"https://orcid.org/0000-0001-6445-3496","observed_orcids":["https://orcid.org/0000-0001-6445-3496"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Navin Gupta","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","Harvard Medical School, Boston, Massachusetts","Co-first authors"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Co-first authors","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081692861","display_name":"Koichiro Susa","orcid":"https://orcid.org/0000-0002-0100-4527","observed_orcids":["https://orcid.org/0000-0002-0100-4527"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Koichiro Susa","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","Harvard Medical School, Boston, Massachusetts","Co-first authors"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Co-first authors","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079574884","display_name":"Yoko Yoda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoko Yoda","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089781525","display_name":"Joseph Vincent Bonventre","orcid":"https://orcid.org/0000-0001-7144-386X","observed_orcids":["https://orcid.org/0000-0001-7144-386X"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210157861","display_name":"Harvard Stem Cell Institute","ror":"https://ror.org/04kj1hn59","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph V. Bonventre","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","Harvard Medical School, Boston, Massachusetts","Harvard Stem Cell Institute, Cambridge, Massachusetts"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Stem Cell Institute, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023793227","display_name":"M. Todd Valerius","orcid":"https://orcid.org/0000-0001-8143-9231","observed_orcids":["https://orcid.org/0000-0001-8143-9231"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210157861","display_name":"Harvard Stem Cell Institute","ror":"https://ror.org/04kj1hn59","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"M. Todd Valerius","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","Harvard Medical School, Boston, Massachusetts","Harvard Stem Cell Institute, Cambridge, Massachusetts","Co-last authors"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Stem Cell Institute, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]},{"raw_affiliation_string":"Co-last authors","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5032821089","display_name":"Ryuji Morizane","orcid":"https://orcid.org/0000-0002-0377-5667","observed_orcids":["https://orcid.org/0000-0002-0377-5667"]},"institutions":[{"id":"https://openalex.org/I1283280774","display_name":"Brigham and Women's Hospital","ror":"https://ror.org/04b6nzv94","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I121934306","https://openalex.org/I1283280774","https://openalex.org/I48633490"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]},{"id":"https://openalex.org/I4210157861","display_name":"Harvard Stem Cell Institute","ror":"https://ror.org/04kj1hn59","country_code":"US","type":"facility","lineage":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ryuji Morizane","raw_affiliation_strings":["Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","Harvard Medical School, Boston, Massachusetts","Harvard Stem Cell Institute, Cambridge, Massachusetts","Co-last authors"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts","institution_ids":["https://openalex.org/I1283280774"]},{"raw_affiliation_string":"Harvard Medical School, Boston, Massachusetts","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Harvard Stem Cell Institute, Cambridge, Massachusetts","institution_ids":["https://openalex.org/I136199984","https://openalex.org/I4210157861"]},{"raw_affiliation_string":"Co-last authors","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":0.5332,"has_fulltext":false,"cited_by_count":19,"citation_normalized_percentile":{"value":0.60763646,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"45","issue":"1","first_page":"e50","last_page":"e50"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.5249000191688538,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.5249000191688538,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.47440001368522644,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9-genome-editing","display_name":"CRISPR/Cas9 genome editing","score":0.9300000071525574},{"id":"https://openalex.org/keywords/human-pluripotent-stem-cells","display_name":"human pluripotent stem cells","score":0.9890000224113464},{"id":"https://openalex.org/keywords/transfection-efficiency","display_name":"transfection efficiency","score":0.9649999737739563},{"id":"https://openalex.org/keywords/pluripotency-maintenance","display_name":"pluripotency maintenance","score":0.8579999804496765},{"id":"https://openalex.org/keywords/gene-knockout","display_name":"gene knockout","score":0.43299999833106995},{"id":"https://openalex.org/keywords/feeder-cell-free-culture","display_name":"feeder cell-free culture","score":0.6869999766349792},{"id":"https://openalex.org/keywords/disease-modeling","display_name":"disease modeling","score":0.5410000085830688}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8911279439926147},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.8590441942214966},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8479399681091309},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8016005754470825},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.6195600628852844},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.536617636680603},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4652702808380127},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.44855839014053345},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.43800097703933716},{"id":"https://openalex.org/C10854531","wikidata":"https://www.wikidata.org/wiki/Q1061415","display_name":"Regenerative medicine","level":3,"score":0.4311257004737854},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.39094147086143494},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3495789170265198},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.32145628333091736}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000076987","descriptor_name":"CRISPR-Associated Protein 9","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005615","descriptor_name":"Freezing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008954","descriptor_name":"Models, Biological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012326","descriptor_name":"RNA Splicing","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D015894","descriptor_name":"Genome, Human","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D039904","descriptor_name":"Pluripotent Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D049109","descriptor_name":"Cell Proliferation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1002/cpsc.50","doi":"https://doi.org/10.1002/cpsc.50","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cpsc.50","pdf_url":null,"source":{"id":"https://openalex.org/S2764651103","display_name":"Current Protocols in Stem Cell Biology","issn_l":"1938-8969","issn":["1938-8969","1941-7322"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1","poland-70"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Stem Cell Biology","raw_type":"journal-article"},{"id":"pmid:30040245","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30040245","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in stem cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:6060633","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6060633","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Protoc Stem Cell Biol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:6060633","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6060633","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Protoc Stem Cell Biol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1591357002","display_name":"Mechanisms of Ischemic Kidney Injury and Repair","funder_award_id":"R37DK039773","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G204946639","display_name":"Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease","funder_award_id":"UH3TR002155","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G3349364425","display_name":"The USC (Re)Building the Kidney Coordinating Center","funder_award_id":"U01DK107350","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G3676009097","display_name":"Training in Academic Nephrology","funder_award_id":"T32DK007527","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G8932233666","display_name":"Kidney Microphysiological Analysis Platforms (MAP) to Optimize Function and Model Disease","funder_award_id":"UG3TR002155","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"},{"id":"https://openalex.org/G9000861971","display_name":"Kidney Injury Molecule-1 in Epithelial Repair","funder_award_id":"R01DK072381","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G9000903649","display_name":"Mechanisms of Ischemic Kidney Injury and Repair","funder_award_id":"R01DK039773","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"},{"id":"https://openalex.org/F4320337472","display_name":"National Center for Advancing Translational Sciences","ror":"https://ror.org/04pw6fb54"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W1599568627","https://openalex.org/W1608084731","https://openalex.org/W1904073439","https://openalex.org/W1905883997","https://openalex.org/W1915704240","https://openalex.org/W1969193847","https://openalex.org/W1969471153","https://openalex.org/W1972564906","https://openalex.org/W1974343902","https://openalex.org/W1977709885","https://openalex.org/W1982048287","https://openalex.org/W1982969769","https://openalex.org/W2000151960","https://openalex.org/W2002791185","https://openalex.org/W2009138958","https://openalex.org/W2013848373","https://openalex.org/W2018286352","https://openalex.org/W2025074364","https://openalex.org/W2025569286","https://openalex.org/W2031522785","https://openalex.org/W2032472678","https://openalex.org/W2036505824","https://openalex.org/W2046591218","https://openalex.org/W2050160133","https://openalex.org/W2050345170","https://openalex.org/W2051255304","https://openalex.org/W2083431317","https://openalex.org/W2084648002","https://openalex.org/W2086943348","https://openalex.org/W2108038398","https://openalex.org/W2110260408","https://openalex.org/W2111900629","https://openalex.org/W2112444085","https://openalex.org/W2125987139","https://openalex.org/W2145782793","https://openalex.org/W2156862506","https://openalex.org/W2164995160","https://openalex.org/W2169567789","https://openalex.org/W2176166968","https://openalex.org/W2192723508","https://openalex.org/W2314225431","https://openalex.org/W2514410885","https://openalex.org/W2564377385","https://openalex.org/W2565256058","https://openalex.org/W2587426589","https://openalex.org/W4234557871","https://openalex.org/W4242353234","https://openalex.org/W4245053291"],"related_works":["https://openalex.org/W2514410885","https://openalex.org/W4310080626","https://openalex.org/W2781815848","https://openalex.org/W1905883997","https://openalex.org/W3194124984","https://openalex.org/W2223567094","https://openalex.org/W2058187928","https://openalex.org/W4411718238","https://openalex.org/W2976267078","https://openalex.org/W2124758693"],"abstract_inverted_index":{"Human":[0],"pluripotent":[1,93],"stem":[2,139],"cells":[3,19,108],"(hPSCs)":[4],"represent":[5],"a":[6,98],"formidable":[7],"tool":[8],"for":[9,35,86],"disease":[10],"modeling,":[11],"drug":[12],"discovery,":[13],"and":[14,20,46,61,89,109,133],"regenerative":[15],"medicine":[16],"using":[17],"human":[18,138],"tissues":[21],"in":[22],"vitro.":[23],"Evolving":[24],"techniques":[25],"of":[26,38,63,103],"targeted":[27],"genome":[28],"editing,":[29],"specifically":[30],"the":[31,36,56],"CRISPR/Cas9":[32],"system,":[33],"allow":[34],"generation":[37],"cell":[39,59,140],"lines":[40,95,141],"bearing":[41],"gene-specific":[42,144],"knock-outs,":[43],"knock-in":[44],"reporters,":[45],"precise":[47],"mutations.":[48,145],"However,":[49],"there":[50],"are":[51,121],"increasing":[52],"concerns":[53],"related":[54],"to":[55,114,136],"transfection":[57,80],"efficiency,":[58,81],"viability,":[60,88],"maintenance":[62],"pluripotency":[64],"provided":[65],"by":[66,148],"genome-editing":[67],"techniques.":[68],"The":[69,124],"procedure":[70],"presented":[71],"here":[72],"employs":[73],"transient":[74],"antibiotic":[75],"selection":[76],"that":[77],"overcomes":[78],"reduced":[79,110],"avoids":[82],"cytotoxic":[83],"flow":[84],"sorting":[85],"increased":[87],"generates":[90],"multiple":[91],"genome-edited":[92],"hPSC":[94],"expanded":[96],"from":[97,106],"single":[99],"parent":[100],"cell.":[101],"Avoidance":[102],"xenogeneic":[104],"contamination":[105],"feeder":[107],"operator":[111],"workload,":[112],"owing":[113],"single-cell":[115],"passaging":[116],"rather":[117],"than":[118],"clump":[119],"passaging,":[120],"additional":[122],"benefits.":[123],"outlined":[125],"methods":[126],"may":[127],"enable":[128],"researchers":[129],"with":[130],"limited":[131],"means":[132],"technical":[134],"experience":[135],"create":[137],"containing":[142],"desired":[143],"\u00a9":[146],"2018":[147],"John":[149],"Wiley":[150],"&":[151],"Sons,":[152],"Inc.":[153]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-05-17T00:00:00"}