{"id":"https://openalex.org/W2802002261","doi":"https://doi.org/10.1002/biot.201700582","title":"Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae","display_name":"Modular Ligation Extension of Guide RNA Operons (LEGO) for Multiplexed dCas9 Regulation of Metabolic Pathways in Saccharomyces cerevisiae","publication_year":2018,"publication_date":"2018-04-16","ids":{"openalex":"https://openalex.org/W2802002261","doi":"https://doi.org/10.1002/biot.201700582","mag":"2802002261","pmid":"https://pubmed.ncbi.nlm.nih.gov/29663663"},"language":"en","primary_location":{"id":"doi:10.1002/biot.201700582","doi":"https://doi.org/10.1002/biot.201700582","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201700582","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086162151","display_name":"Matthew Deaner","orcid":"https://orcid.org/0000-0001-9490-8295","observed_orcids":["https://orcid.org/0000-0001-9490-8295"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Matthew Deaner","raw_affiliation_strings":["McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075867003","display_name":"Allison Holzman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Allison Holzman","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5023917549","display_name":"Hal S. Alper","orcid":"https://orcid.org/0000-0002-8246-8605","observed_orcids":["https://orcid.org/0000-0002-8246-8605"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Hal S. Alper","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712","McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, The University of Texas at Austin, 2500 Speedway Avenue, Austin, TX 78712","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"McKetta Department of Chemical Engineering, The University of Texas at Austin, 200 E Dean Keeton St. Stop C0400, Austin, TX 78712","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5023917549"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":4780,"currency":"USD","value_usd":4780},"apc_paid":null,"fwci":1.2226,"has_fulltext":false,"cited_by_count":34,"citation_normalized_percentile":{"value":0.78000163,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"13","issue":"9","first_page":"e1700582","last_page":"e1700582"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8705999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.8705999851226807,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.12430000305175781,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0013000000035390258,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dcas9","display_name":"dCas9","score":0.7039999961853027},{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.7429999709129333},{"id":"https://openalex.org/keywords/type-iis-restriction-enzymes","display_name":"type IIS restriction enzymes","score":0.6069999933242798},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.6539999842643738},{"id":"https://openalex.org/keywords/2-3-butanediol-production","display_name":"2,3-butanediol production","score":0.9419999718666077},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9660000205039978}],"concepts":[{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5898981690406799},{"id":"https://openalex.org/C76346623","wikidata":"https://www.wikidata.org/wiki/Q205858","display_name":"Ribozyme","level":4,"score":0.5690020322799683},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.5515810251235962},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.49624544382095337},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.48532170057296753},{"id":"https://openalex.org/C145290725","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome engineering","level":5,"score":0.4313572645187378},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.4263862073421478},{"id":"https://openalex.org/C153957851","wikidata":"https://www.wikidata.org/wiki/Q201448","display_name":"Transfer RNA","level":4,"score":0.4111662805080414},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.3984626829624176},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3732442259788513},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3432316780090332},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3036451041698456}],"mesh":[{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009876","descriptor_name":"Operon","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012441","descriptor_name":"Saccharomyces cerevisiae","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D053858","descriptor_name":"Metabolic Networks and Pathways","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D060847","descriptor_name":"Metabolic Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/biot.201700582","doi":"https://doi.org/10.1002/biot.201700582","is_oa":false,"landing_page_url":"https://doi.org/10.1002/biot.201700582","pdf_url":null,"source":{"id":"https://openalex.org/S31411064","display_name":"Biotechnology Journal","issn_l":"1860-6768","issn":["1860-6768","1860-7314"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology Journal","raw_type":"journal-article"},{"id":"pmid:29663663","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29663663","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology journal","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1224080286","display_name":null,"funder_award_id":"FA9550\u201014\u20101\u20100089","funder_id":"https://openalex.org/F4320338279","funder_display_name":"Air Force Office of Scientific Research"}],"funders":[{"id":"https://openalex.org/F4320338279","display_name":"Air Force Office of Scientific Research","ror":"https://ror.org/011e9bt93"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1553706797","https://openalex.org/W1594078235","https://openalex.org/W1731173485","https://openalex.org/W1968165219","https://openalex.org/W1975833723","https://openalex.org/W1975837950","https://openalex.org/W1990226486","https://openalex.org/W1992783297","https://openalex.org/W2028543816","https://openalex.org/W2035420256","https://openalex.org/W2087846512","https://openalex.org/W2097666362","https://openalex.org/W2101749257","https://openalex.org/W2119635233","https://openalex.org/W2120070569","https://openalex.org/W2127862779","https://openalex.org/W2136536485","https://openalex.org/W2160051211","https://openalex.org/W2163173465","https://openalex.org/W2196670136","https://openalex.org/W2217013086","https://openalex.org/W2238012416","https://openalex.org/W2295025783","https://openalex.org/W2305149643","https://openalex.org/W2313697469","https://openalex.org/W2319568809","https://openalex.org/W2506034145","https://openalex.org/W2526680245","https://openalex.org/W2549316307","https://openalex.org/W2565333691","https://openalex.org/W2587930348","https://openalex.org/W2588990408","https://openalex.org/W2599297560","https://openalex.org/W2608740692","https://openalex.org/W2623910725","https://openalex.org/W2735008767","https://openalex.org/W2769160949","https://openalex.org/W2791931465"],"related_works":["https://openalex.org/W3205454981","https://openalex.org/W4250686445","https://openalex.org/W4362062054","https://openalex.org/W3159035331","https://openalex.org/W2587930348","https://openalex.org/W2800078449","https://openalex.org/W2794081335","https://openalex.org/W7073942989","https://openalex.org/W3047419816","https://openalex.org/W2920169518"],"abstract_inverted_index":{"Metabolic":[0],"engineering":[1],"typically":[2],"utilizes":[3],"a":[4,13,62,89,102,142,164,171],"suboptimal":[5],"step-wise":[6],"gene":[7,27,42],"target":[8],"optimization":[9],"approach":[10,128,160],"to":[11,38,47,50,104],"parse":[12],"highly":[14],"connected":[15],"and":[16,52,66,85,94,100],"regulated":[17],"cellular":[18],"metabolism.":[19],"While":[20],"the":[21,69],"endonuclease-null":[22],"CRISPR/Cas":[23],"system":[24,65],"has":[25,34],"enabled":[26],"expression":[28,64,87],"perturbations":[29],"without":[30],"genetic":[31,168],"modification,":[32],"it":[33],"been":[35],"mostly":[36],"limited":[37],"small":[39],"sets":[40],"of":[41,71,91,108,123,132],"targets":[43],"in":[44,149],"eukaryotes":[45],"due":[46],"inefficient":[48],"methods":[49],"assemble":[51],"express":[53],"large":[54,133],"sgRNA":[55,93,124],"operons.":[56,135],"In":[57],"this":[58,138],"work,":[59],"we":[60,98],"develop":[61],"TEF1p-tRNA":[63],"demonstrate":[67,137,157],"that":[68,158],"use":[70],"tRNAs":[72],"as":[73,163],"splicing":[74],"elements":[75],"flanking":[76],"sgRNAs":[77],"provides":[78],"higher":[79],"efficiency":[80],"than":[81],"both":[82],"Pol":[83],"III":[84],"ribozyme-based":[86],"across":[88],"variety":[90],"single":[92],"multiplexed":[95],"contexts.":[96],"Next,":[97],"devise":[99],"validate":[101],"scheme":[103],"allow":[105],"modular":[106],"construction":[107,131],"tRNA-sgRNA":[109],"(TST)":[110],"operons":[111],"using":[112],"an":[113],"iterative":[114],"Type":[115],"IIs":[116],"digestion/ligation":[117],"extension":[118],"approach,":[119],"termed":[120],"CRISPR-Ligation":[121],"Extension":[122],"Operons":[125],"(LEGO).":[126],"This":[127],"enables":[129],"facile":[130],"TST":[134],"We":[136],"utility":[139],"by":[140],"constructing":[141],"metabolic":[143],"rewiring":[144],"prototype":[145],"for":[146,166],"2,3-butanediol":[147],"production":[148],"2":[150],"distinct":[151],"yeast":[152],"strain":[153],"backgrounds.":[154],"These":[155],"results":[156],"our":[159],"can":[161],"act":[162],"surrogate":[165],"traditional":[167],"modification":[169],"on":[170],"much":[172],"shorter":[173],"design-cycle":[174],"timescale.":[175]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2018-05-17T00:00:00"}