{"id":"https://openalex.org/W2797697010","doi":"https://doi.org/10.1149/ma2018-01/35/2060","title":"Bioinspired Electrochemical Mesoporous Membrane Platform Enabling Continuous Protein Separation","display_name":"Bioinspired Electrochemical Mesoporous Membrane Platform Enabling Continuous Protein Separation","publication_year":2018,"publication_date":"2018-04-13","ids":{"openalex":"https://openalex.org/W2797697010","doi":"https://doi.org/10.1149/ma2018-01/35/2060","mag":"2797697010"},"language":"en","primary_location":{"id":"doi:10.1149/ma2018-01/35/2060","doi":"https://doi.org/10.1149/ma2018-01/35/2060","is_oa":false,"landing_page_url":"https://doi.org/10.1149/ma2018-01/35/2060","pdf_url":null,"source":{"id":"https://openalex.org/S4210168594","display_name":"ECS Meeting Abstracts","issn_l":"1091-8213","issn":["1091-8213","2151-2035","2151-2043","2152-8365"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311669","host_organization_name":"Institute of Physics","host_organization_lineage":["https://openalex.org/P4310311669"],"host_organization_lineage_names":["Institute of Physics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ECS Meeting Abstracts","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2797697010"],"child_works_count":0,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022411638","display_name":"Daniel E. Shea","orcid":"https://orcid.org/0000-0002-0351-1629","observed_orcids":["https://orcid.org/0000-0002-0351-1629"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel E Shea","raw_affiliation_strings":["Univ. of Washington"],"raw_orcid":"https://orcid.org/0000-0002-0351-1629","affiliations":[{"raw_affiliation_string":"Univ. of Washington","institution_ids":["https://openalex.org/I201448701"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028652380","display_name":"Bruce Jackson Hinds","orcid":"https://orcid.org/0000-0001-9551-6584","observed_orcids":["https://orcid.org/0000-0001-9551-6584"]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Bruce J Hinds","raw_affiliation_strings":["Univ. of Washington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Univ. of Washington","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"MA2018-01","issue":"35","first_page":"2060","last_page":"2060"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11582","display_name":"Nanopore and Nanochannel Transport Studies","score":0.2921999990940094,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11582","display_name":"Nanopore and Nanochannel Transport Studies","score":0.2921999990940094,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12340","display_name":"Anodic Oxide Films and Nanostructures","score":0.2703000009059906,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10412","display_name":"Microfluidic and Capillary Electrophoresis Applications","score":0.21199999749660492,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-separation","display_name":"protein separation","score":0.7649999856948853},{"id":"https://openalex.org/keywords/steric-blocking","display_name":"steric blocking","score":0.7139999866485596},{"id":"https://openalex.org/keywords/imidazole-elution","display_name":"imidazole elution","score":0.8679999709129333},{"id":"https://openalex.org/keywords/electrophoretic-transport","display_name":"electrophoretic transport","score":0.7609999775886536},{"id":"https://openalex.org/keywords/continuous-separation","display_name":"continuous separation","score":0.5479999780654907}],"concepts":[{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.6074801087379456},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.551701545715332},{"id":"https://openalex.org/C82776694","wikidata":"https://www.wikidata.org/wiki/Q380049","display_name":"Mesoporous material","level":3,"score":0.5315202474594116},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.46447646617889404},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4144446849822998},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.40317440032958984},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.32866746187210083},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28574883937835693},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.2602033019065857},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1232081949710846},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1149/ma2018-01/35/2060","doi":"https://doi.org/10.1149/ma2018-01/35/2060","is_oa":false,"landing_page_url":"https://doi.org/10.1149/ma2018-01/35/2060","pdf_url":null,"source":{"id":"https://openalex.org/S4210168594","display_name":"ECS Meeting Abstracts","issn_l":"1091-8213","issn":["1091-8213","2151-2035","2151-2043","2152-8365"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310311669","host_organization_name":"Institute of Physics","host_organization_lineage":["https://openalex.org/P4310311669"],"host_organization_lineage_names":["Institute of Physics"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ECS Meeting Abstracts","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1971656521","https://openalex.org/W2160755498","https://openalex.org/W4233088337","https://openalex.org/W2050851515","https://openalex.org/W2596019539","https://openalex.org/W3165294351","https://openalex.org/W1994462764","https://openalex.org/W2007159777","https://openalex.org/W2071222564","https://openalex.org/W1513235871"],"abstract_inverted_index":{"Protein":[0],"purification":[1,28],"is":[2,87,160,193,317,345],"an":[3,174,258],"increasingly":[4],"important":[5],"problem":[6],"in":[7,121,177,220,426,475],"the":[8,74,103,115,122,127,133,136,145,148,152,164,200,217,221,233,241,252,255,267,270,273,279,287,306,310,313,321,325,328,338,342,350,354,363,367,370,389,417,435,476,495],"current":[9],"era":[10],"of":[11,24,76,100,264,269,286,301,324,337,353,366,385,413,466,517],"bioengineering,":[12],"medicine,":[13],"and":[14,44,52,117,131,159,187,209,224,282,340,378,423,447,463,479,483,488,499,530],"biological":[15],"research.":[16],"Modern":[17],"biomanufacturing":[18],"schemes":[19,33],"attribute":[20],"up":[21],"to":[22,27,114,140,162,215,266,278,348,410,507],"80%":[23],"process":[25,442],"costs":[26],"processes.":[29],"Unfortunately,":[30],"existing":[31],"separation":[32,381,441,449,496],"rely":[34],"on":[35,232,240],"batch-process":[36],"chromatography":[37,204,414],"columns":[38],"that":[39,66,96,124,182],"are":[40,170,471],"diffusion":[41],"mass":[42],"transport-limited":[43],"have":[45],"already":[46],"reached":[47],"optimal":[48],"maximum":[49],"loading":[50],"densities":[51],"throughputs":[53],"for":[54,206,383,402,444],"bioseparations.":[55],"[1]":[56,512],"Our":[57],"present":[58],"work":[59,419,432],"improves":[60],"a":[61,109,178,294,380,399,403,427,438],"previously-demonstrated":[62],"bioinspired":[63],"separations":[64,85],"concept":[65,374,436],"utilizes":[67],"chemically":[68],"functionalized,":[69],"mesoporous,":[70],"gold":[71,228],"electrodes":[72,231],"decorating":[73],"surface":[75,268,330,352],"anodized":[77],"alumina":[78,175,234],"membranes":[79],"with":[80,236,331],"well-controlled":[81],"pore":[82,238],"sizes.":[83],"This":[84,191,373,431],"platform":[86],"inspired":[88],"by":[89,195,473],"cellular":[90,149],"transmembrane":[91,299,391],"ATP-binding":[92],"cassette":[93],"(ABC)":[94],"transporters":[95],"enable":[97],"selective":[98,207],"transport":[99,222,263,300,490],"nutrients":[101],"across":[102,173],"cell":[104,457],"membrane.":[105,271],"In":[106,166,251,309],"ABC":[107],"transporters,":[108],"nutrient":[110,142,146],"first":[111,253],"binds":[112],"selectively":[113,171],"transporter":[116,123,128,137,153],"induces":[118,335],"conformational":[119],"changes":[120],"effectively":[125],"blocks":[126],"active":[129],"site":[130],"closes":[132],"transporter.":[134],"Subsequently,":[135],"hydrolyzes":[138],"ATP":[139],"induce":[141,283],"release,":[143],"eluting":[144],"into":[147,155,437],"cytoplasm.":[150],"Finally,":[151],"reforms":[154],"its":[156],"original":[157],"conformation":[158],"ready":[161],"repeat":[163],"cycle.":[165,308],"our":[167],"system,":[168],"proteins":[169,265,276,291,304],"separated":[172],"membrane":[176,235,274,326,329,355,371,407,477],"similar":[179],"two-step":[180],"fashion":[181],"alternates":[183],"between":[184],"\u201copen\u201d":[185],"pores":[186,356],"sterically":[188],"\u201cclosed\u201d":[189],"pores.":[190,372],"system":[192],"enabled":[194],"three":[196],"critical":[197],"components:":[198],"(1)":[199],"histidine":[201],"tag-based":[202],"affinity":[203,226,280],"chemistry":[205,281],"binding":[208,256,307,482],"elution,":[210],"(2)":[211],"timed":[212],"electrochemical":[213,259],"pulses":[214],"trigger":[216],"two":[218],"steps":[219],"cycle,":[223],"(3)":[225],"chemistry-modified,":[227],"nanoscale":[229],"mesoporous":[230],"characteristic":[237],"diameter":[239],"same":[242],"size":[243,248],"scale":[244],"as":[245,408],"target":[246,290],"protein":[247,339,360,392],"(typically":[249],"5-10nm).":[250],"step,":[254,257,312,315],"pulse":[260],"triggers":[261],"electrophoretic":[262,489],"At":[272],"surface,":[275],"bind":[277],"steric":[284,295],"blocking":[285,298],"mesopores.":[288],"Captured":[289],"simultaneously":[292],"create":[293],"hindrance":[296],"effect,":[297],"non-specifically":[302],"bound":[303,332],"during":[305],"second":[311],"elution":[314,336],"imidazole":[316,343],"electrophoretically":[318],"pumped":[319],"from":[320,502],"permeate":[322],"side":[323],"towards":[327],"protein.":[333],"Imidazole":[334],"thus":[341],"concentration":[344],"carefully":[346],"controlled":[347],"keep":[349],"top":[351],"blocked":[357],"while":[358],"facilitating":[359],"release":[361,484],"at":[362],"bottom":[364],"edge":[365],"electrode":[368],"inside":[369],"was":[375,420,424],"successfully":[376],"demonstrated":[377],"showed":[379],"factor":[382,497],"GFP:BSA":[384],"16:1":[386],"(mass/mass).":[387],"Additionally,":[388],"net":[390],"flux":[393,501],"(320":[394],"ng":[395,504,509],"cm-2":[396,505,510],"hr-1)":[397],"yields":[398],"daily":[400],"throughput":[401,446],"0.75":[404],"cm2":[405],"area":[406],"comparable":[409],"1":[411],"mL":[412],"resin.":[415],"However,":[416],"prior":[418],"not":[421],"optimized":[422,443],"performed":[425],"batch":[428],"operation.":[429],"[2]":[430,523],"further":[433],"develops":[434],"scalable,":[439],"continuous":[440,459],"increased":[445,500],"improved":[448,494],"factor.":[450],"A":[451],"laser-machined,":[452],"thermally-fused":[453],"thermoplastic":[454],"acrylic":[455],"flow":[456],"enables":[458],"operation,":[460],"pH":[461],"control,":[462],"finer":[464],"control":[465],"operational":[467],"details.":[468],"Process":[469],"optimizations":[470,493],"realized":[472],"improvements":[474],"fabrication":[478],"preparation":[480],"protocol,":[481],"reaction":[485],"kinetics":[486],"modeling,":[487],".":[491],"The":[492],">20":[498],"320":[503],"hr-1":[506],">1000":[508],"hr-1.":[511],"S.":[513,514],"Farid,":[515],"Journal":[516],"Chromatography":[518],"B,":[519],"848,":[520],"8\u201318":[521],"(2007).":[522],"Z.":[524],"Chen,":[525,527],"T.":[526],"X.":[528],"Sun,":[529],"B.":[531],"J.":[532],"Hinds,":[533],"Advanced":[534],"Functional":[535],"Materials,":[536],"24,":[537],"4317\u20134323":[538],"(2014).":[539]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2018-04-24T00:00:00"}