{"id":"https://openalex.org/W2794533488","doi":"https://doi.org/10.1007/978-1-4939-7759-8_4","title":"Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip","display_name":"Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip","publication_year":2018,"publication_date":"2018-01-01","ids":{"openalex":"https://openalex.org/W2794533488","doi":"https://doi.org/10.1007/978-1-4939-7759-8_4","mag":"2794533488","pmid":"https://pubmed.ncbi.nlm.nih.gov/29605908"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-4939-7759-8_4","doi":"https://doi.org/10.1007/978-1-4939-7759-8_4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-7759-8_4","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W4253565130","display_name":"Protein Complex Assembly"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5098972963","display_name":"Sandip Kaledhonkar","orcid":"https://orcid.org/0000-0003-3264-9878","observed_orcids":["https://orcid.org/0000-0003-3264-9878"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sandip Kaledhonkar","raw_affiliation_strings":["Department of Biochemistry and Molecular Biophysics, Columbia University, New York, 10032, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, 10032, NY, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006396432","display_name":"Ziao Fu","orcid":"https://orcid.org/0000-0001-5036-1945","observed_orcids":["https://orcid.org/0000-0001-5036-1945"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ziao Fu","raw_affiliation_strings":["Integrated Program in Cellular, Molecular and Biomolecular Studies, Columbia University College of Physicians and Surgeons, New York, 10032, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Integrated Program in Cellular, Molecular and Biomolecular Studies, Columbia University College of Physicians and Surgeons, New York, 10032, NY, USA","institution_ids":["https://openalex.org/I78577930"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029073594","display_name":"Howard D. White","orcid":"https://orcid.org/0000-0003-1066-2051","observed_orcids":["https://orcid.org/0000-0003-1066-2051"]},"institutions":[{"id":"https://openalex.org/I156802259","display_name":"Eastern Virginia Medical School","ror":"https://ror.org/056hr4255","country_code":"US","type":"education","lineage":["https://openalex.org/I156802259"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Howard White","raw_affiliation_strings":["Physiological Sciences, Eastern Virginia Medical School, Norfolk, 23507, VA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiological Sciences, Eastern Virginia Medical School, Norfolk, 23507, VA, USA","institution_ids":["https://openalex.org/I156802259"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5042892654","display_name":"Joachim Frank","orcid":"https://orcid.org/0000-0001-5449-6943","observed_orcids":["https://orcid.org/0000-0001-5449-6943","https://orcid.org/0000-0002-2442-1112"]},"institutions":[{"id":"https://openalex.org/I78577930","display_name":"Columbia University","ror":"https://ror.org/00hj8s172","country_code":"US","type":"education","lineage":["https://openalex.org/I78577930"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Joachim Frank","raw_affiliation_strings":["Department of Biochemistry and Molecular Biophysics, Columbia University, New York, 10032, NY, USA. jf2192@cumc.columbia.edu","Department of Biological Sciences, Columbia University, New York, 10027, NY, USA. jf2192@cumc.columbia.edu","Department of Biological Sciences, Columbia University, New York, 10027, NY, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biophysics, Columbia University, New York, 10032, NY, USA. jf2192@cumc.columbia.edu","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Biological Sciences, Columbia University, New York, 10027, NY, USA. jf2192@cumc.columbia.edu","institution_ids":["https://openalex.org/I78577930"]},{"raw_affiliation_string":"Department of Biological Sciences, Columbia University, New York, 10027, NY, USA","institution_ids":["https://openalex.org/I78577930"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5042892654"],"corresponding_institution_ids":["https://openalex.org/I78577930"],"apc_list":null,"apc_paid":null,"fwci":16.9951,"has_fulltext":false,"cited_by_count":40,"citation_normalized_percentile":{"value":1.0,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"1764","issue":null,"first_page":"59","last_page":"71"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microfluidics","display_name":"microfluidics","score":0.9800000190734863},{"id":"https://openalex.org/keywords/kinetic-experiments","display_name":"kinetic experiments","score":0.3840000033378601},{"id":"https://openalex.org/keywords/intermediate-state","display_name":"intermediate state","score":0.609000027179718},{"id":"https://openalex.org/keywords/millisecond-time-scale","display_name":"millisecond time scale","score":0.7369999885559082},{"id":"https://openalex.org/keywords/direct-electron-detectors","display_name":"direct electron detectors","score":0.6909999847412109},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.609000027179718}],"concepts":[{"id":"https://openalex.org/C60327585","wikidata":"https://www.wikidata.org/wiki/Q723733","display_name":"Millisecond","level":2,"score":0.8541415929794312},{"id":"https://openalex.org/C20702342","wikidata":"https://www.wikidata.org/wiki/Q5190506","display_name":"Cryo-electron microscopy","level":2,"score":0.7265375852584839},{"id":"https://openalex.org/C8673954","wikidata":"https://www.wikidata.org/wiki/Q138845","display_name":"Microfluidics","level":2,"score":0.5309065580368042},{"id":"https://openalex.org/C138777275","wikidata":"https://www.wikidata.org/wiki/Q6884054","display_name":"Mixing (physics)","level":2,"score":0.5279820561408997},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.49188971519470215},{"id":"https://openalex.org/C94915269","wikidata":"https://www.wikidata.org/wiki/Q1834857","display_name":"Detector","level":2,"score":0.4706539213657379},{"id":"https://openalex.org/C2778755073","wikidata":"https://www.wikidata.org/wiki/Q10858537","display_name":"Scale (ratio)","level":2,"score":0.465224951505661},{"id":"https://openalex.org/C198531522","wikidata":"https://www.wikidata.org/wiki/Q485146","display_name":"Sample (material)","level":2,"score":0.45044058561325073},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.4383810758590698},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.4282488226890564},{"id":"https://openalex.org/C147120987","wikidata":"https://www.wikidata.org/wiki/Q2225","display_name":"Electron","level":2,"score":0.41655072569847107},{"id":"https://openalex.org/C93877712","wikidata":"https://www.wikidata.org/wiki/Q132560","display_name":"Electron microscope","level":2,"score":0.41470903158187866},{"id":"https://openalex.org/C159467904","wikidata":"https://www.wikidata.org/wiki/Q2001702","display_name":"Chemical physics","level":1,"score":0.32047855854034424},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2554929554462433},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.18392863869667053},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.1630522608757019},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.07530173659324646},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.07488390803337097},{"id":"https://openalex.org/C46141821","wikidata":"https://www.wikidata.org/wiki/Q209402","display_name":"Nuclear magnetic resonance","level":1,"score":0.07241332530975342},{"id":"https://openalex.org/C1276947","wikidata":"https://www.wikidata.org/wiki/Q333","display_name":"Astronomy","level":1,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008968","descriptor_name":"Molecular Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000648","qualifier_name":"ultrastructure","is_major_topic":true},{"descriptor_ui":"D020285","descriptor_name":"Cryoelectron Microscopy","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D020285","descriptor_name":"Cryoelectron Microscopy","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D044085","descriptor_name":"Microfluidics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D046208","descriptor_name":"Microchip Analytical Procedures","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-4939-7759-8_4","doi":"https://doi.org/10.1007/978-1-4939-7759-8_4","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4939-7759-8_4","pdf_url":null,"source":{"id":"https://openalex.org/S4210172139","display_name":"Methods in molecular biology","issn_l":"1064-3745","issn":["1064-3745","1940-6029"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3","vabb-shw"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in Molecular Biology","raw_type":"book-chapter"},{"id":"pmid:29605908","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29605908","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Methods in molecular biology (Clifton, N.J.)","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1539491410","https://openalex.org/W1979864007","https://openalex.org/W1994306051","https://openalex.org/W2010359172","https://openalex.org/W2076449818","https://openalex.org/W2078668410","https://openalex.org/W2078726019","https://openalex.org/W2104234755","https://openalex.org/W2140426008","https://openalex.org/W2148941629","https://openalex.org/W2548199696","https://openalex.org/W2592535877","https://openalex.org/W2593511418","https://openalex.org/W2950967305","https://openalex.org/W2953320394"],"related_works":["https://openalex.org/W4415606977","https://openalex.org/W4385349011","https://openalex.org/W7129365020","https://openalex.org/W3046393576","https://openalex.org/W4308869048","https://openalex.org/W4389767599","https://openalex.org/W2021063875","https://openalex.org/W4385072053","https://openalex.org/W3042080880","https://openalex.org/W7130553609"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":9},{"year":2019,"cited_by_count":8},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2018-04-06T00:00:00"}