{"id":"https://openalex.org/W2776833389","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","title":"CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line.","display_name":"CRISPR/Cas9, a new approach to successful knockdown of ABCB1/P-glycoprotein and reversal of chemosensitivity in human epithelial ovarian cancer cell line.","publication_year":2018,"publication_date":"2018-02-01","ids":{"openalex":"https://openalex.org/W2776833389","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","mag":"2776833389","pmid":"https://pubmed.ncbi.nlm.nih.gov/29456815","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5811757"},"language":"en","primary_location":{"id":"pmh:oai:pubmedcentral.nih.gov:5811757","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iran J Basic Med Sci","raw_type":"Text"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/pdf/","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081814101","display_name":"Leyla Norouzi\u2010Barough","orcid":"https://orcid.org/0000-0002-8699-9665","observed_orcids":["https://orcid.org/0000-0002-8699-9665"]},"institutions":[{"id":"https://openalex.org/I64532579","display_name":"Qazvin University of Medical Sciences","ror":"https://ror.org/04sexa105","country_code":"IR","type":"education","lineage":["https://openalex.org/I4210110574","https://openalex.org/I64532579"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Leyla Norouzi-Barough","raw_affiliation_strings":["Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran","institution_ids":["https://openalex.org/I64532579"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113781995","display_name":"Mohammad Reza Sarookhani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I64532579","display_name":"Qazvin University of Medical Sciences","ror":"https://ror.org/04sexa105","country_code":"IR","type":"education","lineage":["https://openalex.org/I4210110574","https://openalex.org/I64532579"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mohammadreza Sarookhani","raw_affiliation_strings":["Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran","institution_ids":["https://openalex.org/I64532579"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101793490","display_name":"Rasoul Salehi","orcid":"https://orcid.org/0000-0003-4062-6166","observed_orcids":["https://orcid.org/0000-0003-4062-6166","https://orcid.org/0000-0002-1868-1735"]},"institutions":[{"id":"https://openalex.org/I114982161","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56","country_code":"IR","type":"education","lineage":["https://openalex.org/I114982161"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Rasoul Salehi","raw_affiliation_strings":["Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran","institution_ids":["https://openalex.org/I114982161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028294663","display_name":"Mohammadreza Sharifi","orcid":"https://orcid.org/0000-0002-1538-9034","observed_orcids":["https://orcid.org/0000-0002-1538-9034"]},"institutions":[{"id":"https://openalex.org/I114982161","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56","country_code":"IR","type":"education","lineage":["https://openalex.org/I114982161"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Mohammadreza Sharifi","raw_affiliation_strings":["Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran","institution_ids":["https://openalex.org/I114982161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5090066273","display_name":"Sahar Moghbelinejad","orcid":"https://orcid.org/0000-0002-7653-8689","observed_orcids":["https://orcid.org/0000-0002-7653-8689"]},"institutions":[{"id":"https://openalex.org/I64532579","display_name":"Qazvin University of Medical Sciences","ror":"https://ror.org/04sexa105","country_code":"IR","type":"education","lineage":["https://openalex.org/I4210110574","https://openalex.org/I64532579"]}],"countries":["IR"],"is_corresponding":false,"raw_author_name":"Sahar Moghbelinejad","raw_affiliation_strings":["Department of Biochemistry and Genetic, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Genetic, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran","institution_ids":["https://openalex.org/I64532579"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.8788,"has_fulltext":true,"cited_by_count":38,"citation_normalized_percentile":{"value":0.88526311,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"21","issue":"2","first_page":"181","last_page":"187"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9660000205039978},{"id":"https://openalex.org/keywords/p-glycoprotein","display_name":"P-glycoprotein","score":0.9990000128746033},{"id":"https://openalex.org/keywords/multidrug-resistance","display_name":"multidrug resistance","score":0.9539999961853027},{"id":"https://openalex.org/keywords/epithelial-ovarian-cancer","display_name":"epithelial ovarian cancer","score":0.890999972820282},{"id":"https://openalex.org/keywords/doxorubicin-sensitivity","display_name":"doxorubicin sensitivity","score":0.9440000057220459},{"id":"https://openalex.org/keywords/single-guide-rna","display_name":"single guide RNA","score":0.7440000176429749},{"id":"https://openalex.org/keywords/gene-knockdown","display_name":"gene knockdown","score":0.9340000152587891},{"id":"https://openalex.org/keywords/abc-transporters","display_name":"ABC transporters","score":0.7860000133514404}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7790813446044922},{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.7223042249679565},{"id":"https://openalex.org/C2781303535","wikidata":"https://www.wikidata.org/wiki/Q18936","display_name":"Doxorubicin","level":3,"score":0.5594712495803833},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5372341275215149},{"id":"https://openalex.org/C133936738","wikidata":"https://www.wikidata.org/wiki/Q643839","display_name":"Multiple drug resistance","level":3,"score":0.515600860118866},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4844091534614563},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.4798409640789032},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.45422983169555664},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4363016188144684},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.4215690493583679},{"id":"https://openalex.org/C2780427987","wikidata":"https://www.wikidata.org/wiki/Q172341","display_name":"Ovarian cancer","level":3,"score":0.4214783310890198},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4163203537464142},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.3631650507450104},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3355870544910431},{"id":"https://openalex.org/C114851261","wikidata":"https://www.wikidata.org/wiki/Q12147416","display_name":"Drug resistance","level":2,"score":0.3197462856769562},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.150374174118042},{"id":"https://openalex.org/C2776694085","wikidata":"https://www.wikidata.org/wiki/Q974135","display_name":"Chemotherapy","level":2,"score":0.14131012558937073},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.13432925939559937}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:pubmedcentral.nih.gov:5811757","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iran J Basic Med Sci","raw_type":"Text"},{"id":"pmh:oai:europepmc.org:4724466","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5811757","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:29456815","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29456815","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iranian journal of basic medical sciences","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:c41466c782a147b68f6de2a3f27c195d","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":true,"landing_page_url":"https://doaj.org/article/c41466c782a147b68f6de2a3f27c195d","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Iranian Journal of Basic Medical Sciences, Vol 21, Iss 2, Pp 181-187 (2018)","raw_type":"article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:5811757","doi":"https://doi.org/10.22038/ijbms.2017.25145.6230","is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC5811757/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Iran J Basic Med Sci","raw_type":"Text"},"sustainable_development_goals":[{"score":0.98580002784729,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322245","display_name":"Isfahan University of Medical Sciences","ror":"https://ror.org/04waqzz56"},{"id":"https://openalex.org/F4320323836","display_name":"Qazvin University of Medical Sciences","ror":"https://ror.org/04sexa105"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2776833389.pdf","grobid_xml":"https://content.openalex.org/works/W2776833389.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W137643597","https://openalex.org/W1804459219","https://openalex.org/W1964709116","https://openalex.org/W1978287605","https://openalex.org/W1979518586","https://openalex.org/W1982739978","https://openalex.org/W1999729424","https://openalex.org/W2014623932","https://openalex.org/W2015030879","https://openalex.org/W2026592619","https://openalex.org/W2032167680","https://openalex.org/W2054340359","https://openalex.org/W2064815984","https://openalex.org/W2065270021","https://openalex.org/W2074033060","https://openalex.org/W2097361033","https://openalex.org/W2112444085","https://openalex.org/W2116553552","https://openalex.org/W2123113463","https://openalex.org/W2134902789","https://openalex.org/W2138935969","https://openalex.org/W2148230829","https://openalex.org/W2152368724","https://openalex.org/W2160051211","https://openalex.org/W2164573650","https://openalex.org/W2178405251","https://openalex.org/W2206690149","https://openalex.org/W2260320054","https://openalex.org/W2289206353","https://openalex.org/W2309334613","https://openalex.org/W2325637194","https://openalex.org/W2329552021","https://openalex.org/W2401564240","https://openalex.org/W2553000813","https://openalex.org/W2559832666","https://openalex.org/W4212835986"],"related_works":["https://openalex.org/W2914104286","https://openalex.org/W2559832666","https://openalex.org/W2553000813","https://openalex.org/W2796350598","https://openalex.org/W4200409203","https://openalex.org/W2097361033","https://openalex.org/W7055359380","https://openalex.org/W4367054883","https://openalex.org/W3118878140","https://openalex.org/W7131752377"],"abstract_inverted_index":{"OBJECTIVES:":[0],"Multidrug":[1],"resistance":[2],"(MDR)":[3],"is":[4,26,174],"a":[5,19,27],"major":[6],"obstacle":[7],"in":[8,33,57,91,149,181,206],"the":[9,43,67,80,127,142,168,177,182,187],"successful":[10],"chemotherapy":[11],"of":[12,16,21,37,47,85,96,108,144,158,170,201,208],"ovarian":[13,61],"cancer.":[14,34],"Inhibition":[15],"P-glycoprotein":[17],"(P-gp),":[18],"member":[20],"ATP-binding":[22],"cassette":[23],"(ABC)":[24],"transporters,":[25],"well-known":[28],"strategy":[29],"to":[30,41,52,70,122,163],"overcome":[31],"MDR":[32],"The":[35,133,146],"aim":[36],"this":[38,92,171],"study":[39],"was":[40,98,124,153],"investigate":[42],"efficiency":[44],"and":[45,65,82,116,190,195],"ability":[46],"CRISPR/Cas9":[48,137],"genome":[49],"editing":[50,200],"technology":[51],"knockdown":[53],"ABCB1":[54,87,97,150],"gene":[55,88,151,189,199],"expression":[56,143,152],"adriamycin":[58],"resistant":[59],"(A2780/ADR)":[60],"cancer":[62],"cell":[63,114,203],"line":[64,115,204],"evaluate":[66],"sensitivity":[68,121,157],"changes":[69],"doxorubicin.":[71,164],"MATERIALS":[72],"AND":[73],"METHODS:":[74],"Three":[75],"single-guide":[76],"RNAs":[77],"(sgRNAs)":[78],"targeting":[79],"fourth":[81],"fifth":[83],"exons":[84],"human":[86],"were":[89],"designed":[90],"study.":[93],"Expression":[94],"level":[95],"detected":[99],"using":[100],"quantitative":[101],"real":[102],"time":[103],"PCR":[104],"(qRT-PCR)":[105],"after":[106],"co-transfection":[107],"all":[109],"three":[110],"sgRNAs":[111,162],"into":[112],"A2780/ADR":[113,202],"subsequent":[117],"antibiotic":[118],"selection.":[119],"Drug":[120],"doxorubicin":[123],"determined":[125],"by":[126],"3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium":[128],"bromide":[129],"(MTT)":[130],"assay.":[131],"RESULTS:":[132],"results":[134,169],"showed":[135],"that":[136,176],"system":[138],"could":[139],"significantly":[140],"reduce":[141],"P-gp.":[145],"dramatic":[147],"decline":[148],"associated":[154],"with":[155,161],"increased":[156],"cells":[159],"transfected":[160],"CONCLUSION:":[165],"Based":[166],"on":[167],"study,":[172,184],"it":[173],"concluded":[175],"CRISPR-based":[178],"systems,":[179],"used":[180],"present":[183],"effectively":[185],"down-regulated":[186],"target":[188],"acted":[191],"as":[192],"an":[193],"ideal":[194],"cost-effective":[196],"tool":[197],"for":[198],"resulting":[205],"restoration":[207],"nonmalignant":[209],"phenotype.":[210]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2018-01-05T00:00:00"}