{"id":"https://openalex.org/W2771385705","doi":"https://doi.org/10.1016/j.stemcr.2017.11.004","title":"A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids","display_name":"A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids","publication_year":2017,"publication_date":"2017-12-07","ids":{"openalex":"https://openalex.org/W2771385705","doi":"https://doi.org/10.1016/j.stemcr.2017.11.004","mag":"2771385705","pmid":"https://pubmed.ncbi.nlm.nih.gov/29233552","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5768885"},"language":"en","primary_location":{"id":"doi:10.1016/j.stemcr.2017.11.004","doi":"https://doi.org/10.1016/j.stemcr.2017.11.004","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.stemcr.2017.11.004","pdf_url":"http://www.cell.com/article/S2213671117304903/pdf","source":{"id":"https://openalex.org/S2764425922","display_name":"Stem Cell Reports","issn_l":"2213-6711","issn":["2213-6711"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Reports","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"http://www.cell.com/article/S2213671117304903/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5001992157","display_name":"Yu Takahashi","orcid":"https://orcid.org/0000-0003-3186-2925","observed_orcids":["https://orcid.org/0000-0003-3186-2925"]},"institutions":[{"id":"https://openalex.org/I4210108598","display_name":"Japan Tobacco (Japan)","ror":"https://ror.org/01xdq1k91","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210108598"]},{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Yu Takahashi","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan. Electronic address: yu.takahashi@jt.com","Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan. Electronic address: yu.takahashi@jt.com","institution_ids":["https://openalex.org/I4210108598","https://openalex.org/I4405286826"]},{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]},{"raw_affiliation_string":"Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","institution_ids":["https://openalex.org/I4210108598"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030226203","display_name":"Shintaro Sato","orcid":"https://orcid.org/0000-0001-5156-3354","observed_orcids":["https://orcid.org/0000-0001-5156-3354"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Shintaro Sato","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Electronic address: shinta@biken.osaka-u.ac.jp","Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan. Electronic address: shinta@biken.osaka-u.ac.jp","institution_ids":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082845406","display_name":"Yosuke Kurashima","orcid":"https://orcid.org/0000-0001-8588-4033","observed_orcids":["https://orcid.org/0000-0001-8588-4033"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yosuke Kurashima","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073156255","display_name":"Tomohisa Yamamoto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210108598","display_name":"Japan Tobacco (Japan)","ror":"https://ror.org/01xdq1k91","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210108598"]},{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tomohisa Yamamoto","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","institution_ids":["https://openalex.org/I4210108598","https://openalex.org/I4405286826"]},{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]},{"raw_affiliation_string":"Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","institution_ids":["https://openalex.org/I4210108598"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5061215235","display_name":"Shiho Kurokawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shiho Kurokawa","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084452013","display_name":"Yoshikazu Yuki","orcid":"https://orcid.org/0000-0001-6574-9184","observed_orcids":["https://orcid.org/0000-0001-6574-9184"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshikazu Yuki","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102745434","display_name":"Naoki Takemura","orcid":"https://orcid.org/0000-0002-8858-9299","observed_orcids":["https://orcid.org/0000-0002-8858-9299"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Naoki Takemura","raw_affiliation_strings":["Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051593432","display_name":"Satoshi Uematsu","orcid":"https://orcid.org/0000-0002-5347-6737","observed_orcids":["https://orcid.org/0000-0002-5347-6737"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Satoshi Uematsu","raw_affiliation_strings":["Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Innate Immune Regulation, International Research and Development Center for Mucosal Vaccine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015271442","display_name":"Chen\u2010Yi Lai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Chen-Yi Lai","raw_affiliation_strings":["Division of Stem Cell Processing, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Stem Cell Processing, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5032378106","display_name":"Makoto Otsu","orcid":"https://orcid.org/0000-0002-9769-0217","observed_orcids":["https://orcid.org/0000-0002-9769-0217"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Otsu","raw_affiliation_strings":["Division of Stem Cell Processing, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Stem Cell Processing, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Hiroshi Matsuno","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroshi Matsuno","raw_affiliation_strings":["Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047044483","display_name":"Hideki Osawa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hideki Osawa","raw_affiliation_strings":["Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027881902","display_name":"Tsunekazu Mizushima","orcid":"https://orcid.org/0000-0002-0825-6823","observed_orcids":["https://orcid.org/0000-0002-0825-6823"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Tsunekazu Mizushima","raw_affiliation_strings":["Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067262609","display_name":"Junichi Nishimura","orcid":"https://orcid.org/0000-0001-8220-3423","observed_orcids":["https://orcid.org/0000-0001-8220-3423"]},"institutions":[{"id":"https://openalex.org/I98285908","display_name":"The University of Osaka","ror":"https://ror.org/035t8zc32","country_code":"JP","type":"education","lineage":["https://openalex.org/I98285908"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Junichi Nishimura","raw_affiliation_strings":["Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka 565-0871, Japan","institution_ids":["https://openalex.org/I98285908"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007274064","display_name":"Mikio Hayashi","orcid":"https://orcid.org/0000-0002-9670-5683","observed_orcids":["https://orcid.org/0000-0002-9670-5683"]},"institutions":[{"id":"https://openalex.org/I4210108598","display_name":"Japan Tobacco (Japan)","ror":"https://ror.org/01xdq1k91","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210108598"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mikio Hayashi","raw_affiliation_strings":["Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","institution_ids":["https://openalex.org/I4210108598"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5104779069","display_name":"Takayuki Yamaguchi","orcid":"https://orcid.org/0000-0001-5176-6792","observed_orcids":["https://orcid.org/0000-0001-5176-6792"]},"institutions":[{"id":"https://openalex.org/I4210108598","display_name":"Japan Tobacco (Japan)","ror":"https://ror.org/01xdq1k91","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210108598"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Takayuki Yamaguchi","raw_affiliation_strings":["Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Japan Tobacco Inc., Central Pharmaceutical Research Institute, 1-1 Murasaki-cho, Takatsuki, Osaka 569-1125, Japan","institution_ids":["https://openalex.org/I4210108598"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087178931","display_name":"Hiroshi Kiyono","orcid":"https://orcid.org/0000-0001-7307-4658","observed_orcids":["https://orcid.org/0000-0001-7307-4658"]},"institutions":[{"id":"https://openalex.org/I4405286826","display_name":"Institute of Medical Science, The University of Tokyo","ror":"https://ror.org/0376e0j29","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286826","https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroshi Kiyono","raw_affiliation_strings":["Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan","institution_ids":["https://openalex.org/I4405286826"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5001992157","https://openalex.org/A5030226203"],"corresponding_institution_ids":["https://openalex.org/I4210108598","https://openalex.org/I4405286826","https://openalex.org/I74801974"],"apc_list":{"value":4190,"currency":"USD","value_usd":4190},"apc_paid":{"value":4190,"currency":"USD","value_usd":4190},"fwci":3.6044,"has_fulltext":false,"cited_by_count":129,"citation_normalized_percentile":{"value":0.94561408,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":100},"biblio":{"volume":"10","issue":"1","first_page":"314","last_page":"328"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/intestinal-organoids","display_name":"intestinal organoids","score":0.9470000267028809},{"id":"https://openalex.org/keywords/human-induced-pluripotent-stem-cells","display_name":"human induced pluripotent stem cells","score":0.8989999890327454},{"id":"https://openalex.org/keywords/organoid-culture","display_name":"organoid culture","score":0.7979999780654907},{"id":"https://openalex.org/keywords/lentiviral-vectors","display_name":"lentiviral vectors","score":0.6880000233650208},{"id":"https://openalex.org/keywords/conditioned-medium","display_name":"conditioned medium","score":0.9710000157356262},{"id":"https://openalex.org/keywords/wnt3a","display_name":"Wnt3a","score":0.9509999752044678},{"id":"https://openalex.org/keywords/fibroblast-growth-factor-2","display_name":"fibroblast growth factor 2","score":0.9679999947547913},{"id":"https://openalex.org/keywords/definitive-endoderm-differentiation","display_name":"definitive endoderm differentiation","score":0.9890000224113464},{"id":"https://openalex.org/keywords/gene-transduction","display_name":"gene transduction","score":0.7070000171661377},{"id":"https://openalex.org/keywords/scaffold-free-culture","display_name":"scaffold-free culture","score":0.8500000238418579},{"id":"https://openalex.org/keywords/gastrointestinal-diseases","display_name":"gastrointestinal diseases","score":0.6060000061988831},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.9829999804496765}],"concepts":[{"id":"https://openalex.org/C31695470","wikidata":"https://www.wikidata.org/wiki/Q11293125","display_name":"Organoid","level":2,"score":0.9697059392929077},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8097052574157715},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.7660670876502991},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6063368320465088},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.5717411637306213},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.5051108002662659},{"id":"https://openalex.org/C10854531","wikidata":"https://www.wikidata.org/wiki/Q1061415","display_name":"Regenerative medicine","level":3,"score":0.4559529423713684},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4517596662044525},{"id":"https://openalex.org/C2777164004","wikidata":"https://www.wikidata.org/wiki/Q331858","display_name":"Endoderm","level":4,"score":0.43781551718711853},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.41604042053222656},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.18599727749824524},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.16115090250968933}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D004847","descriptor_name":"Epithelial Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007413","descriptor_name":"Intestinal Mucosa","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D007413","descriptor_name":"Intestinal Mucosa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009940","descriptor_name":"Organoids","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D009940","descriptor_name":"Organoids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057026","descriptor_name":"Induced Pluripotent Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D057026","descriptor_name":"Induced Pluripotent Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D060509","descriptor_name":"Wnt3A Protein","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D060509","descriptor_name":"Wnt3A Protein","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.stemcr.2017.11.004","doi":"https://doi.org/10.1016/j.stemcr.2017.11.004","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.stemcr.2017.11.004","pdf_url":"http://www.cell.com/article/S2213671117304903/pdf","source":{"id":"https://openalex.org/S2764425922","display_name":"Stem Cell Reports","issn_l":"2213-6711","issn":["2213-6711"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Reports","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:5768885","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5768885","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Reports","raw_type":"Text"},{"id":"pmid:29233552","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29233552","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem cell reports","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:c4006f10f2d34228a3837a3832bd0c9b","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/c4006f10f2d34228a3837a3832bd0c9b","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Stem Cell Reports, Vol 10, Iss 1, Pp 314-328 (2018)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.stemcr.2017.11.004","doi":"https://doi.org/10.1016/j.stemcr.2017.11.004","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.stemcr.2017.11.004","pdf_url":"http://www.cell.com/article/S2213671117304903/pdf","source":{"id":"https://openalex.org/S2764425922","display_name":"Stem Cell Reports","issn_l":"2213-6711","issn":["2213-6711"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","oarl","poland-140","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stem Cell Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9391000270843506,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G140024321","display_name":null,"funder_award_id":"25860353","funder_id":"https://openalex.org/F4320320912","funder_display_name":"Ministry of Education, Culture, Sports, Science and Technology"},{"id":"https://openalex.org/G2090642964","display_name":null,"funder_award_id":"23229004","funder_id":"https://openalex.org/F4320320912","funder_display_name":"Ministry of Education, Culture, Sports, Science and Technology"},{"id":"https://openalex.org/G7654684222","display_name":"Analysis of mesenchymal network for the mucosal healing","funder_award_id":"16H06243","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"}],"funders":[{"id":"https://openalex.org/F4320311405","display_name":"Japan Agency for Medical Research and Development","ror":"https://ror.org/004rtk039"},{"id":"https://openalex.org/F4320320912","display_name":"Ministry of Education, Culture, Sports, Science and Technology","ror":"https://ror.org/048rj2z13"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2771385705.pdf"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1496521029","https://openalex.org/W1589789540","https://openalex.org/W1601897860","https://openalex.org/W1910612799","https://openalex.org/W1965157206","https://openalex.org/W1979475230","https://openalex.org/W1986751568","https://openalex.org/W1988852350","https://openalex.org/W1995526374","https://openalex.org/W2002513358","https://openalex.org/W2010420220","https://openalex.org/W2014750054","https://openalex.org/W2020755558","https://openalex.org/W2025569286","https://openalex.org/W2031522785","https://openalex.org/W2034540804","https://openalex.org/W2036414872","https://openalex.org/W2044025778","https://openalex.org/W2045055151","https://openalex.org/W2048321002","https://openalex.org/W2052891178","https://openalex.org/W2055777218","https://openalex.org/W2055860761","https://openalex.org/W2071986963","https://openalex.org/W2074636496","https://openalex.org/W2104209550","https://openalex.org/W2105504226","https://openalex.org/W2126493828","https://openalex.org/W2128085444","https://openalex.org/W2134202966","https://openalex.org/W2143149122","https://openalex.org/W2143296478","https://openalex.org/W2146167376","https://openalex.org/W2147864074","https://openalex.org/W2157010562","https://openalex.org/W2159582900","https://openalex.org/W2159714953","https://openalex.org/W2167380395","https://openalex.org/W2170409651","https://openalex.org/W2195777839","https://openalex.org/W2233386492","https://openalex.org/W2312814902","https://openalex.org/W2485841399","https://openalex.org/W2510944967","https://openalex.org/W2645361946","https://openalex.org/W2684461861","https://openalex.org/W2740550497"],"related_works":["https://openalex.org/W2895949672","https://openalex.org/W3169200140","https://openalex.org/W4391278013","https://openalex.org/W2511303792","https://openalex.org/W3087000711","https://openalex.org/W3149503624","https://openalex.org/W7126334754","https://openalex.org/W2994915555","https://openalex.org/W2575557755","https://openalex.org/W2024681578"],"abstract_inverted_index":{"Gut":[0],"epithelial":[1],"organoids":[2,46,73,135],"are":[3,14],"routinely":[4],"used":[5],"to":[6,17,24,57,87,129],"investigate":[7],"intestinal":[8,45,66,72,134],"biology;":[9],"however,":[10],"current":[11],"culture":[12,36,117],"methods":[13],"not":[15],"amenable":[16],"genetic":[18],"manipulation,":[19],"and":[20,60,82,123,132,137,148],"it":[21],"is":[22],"difficult":[23],"generate":[25],"sufficient":[26],"numbers":[27],"for":[28,64,120,151],"high-throughput":[29,143],"studies.":[30],"Here,":[31],"we":[32,102],"present":[33],"an":[34,104],"improved":[35],"system":[37],"of":[38,100,107,145],"human":[39,65,75],"induced":[40],"pluripotent":[41],"stem":[42],"cell":[43],"(iPSC)-derived":[44],"involving":[47],"four":[48],"methodological":[49],"advances.":[50],"(1)":[51],"We":[52,70,113],"adopted":[53],"a":[54],"lentiviral":[55],"vector":[56],"readily":[58,136],"establish":[59],"optimize":[61],"conditioned":[62],"medium":[63],"organoid":[67,116],"culture.":[68],"(2)":[69],"obtained":[71],"from":[74],"iPSCs":[76],"more":[77],"efficiently":[78],"by":[79,98],"supplementing":[80],"WNT3A":[81],"fibroblast":[83],"growth":[84],"factor":[85],"2":[86],"induce":[88],"differentiation":[89],"into":[90],"definitive":[91],"endoderm.":[92],"(3)":[93],"Using":[94],"2D":[95],"culture,":[96],"followed":[97],"re-establishment":[99],"organoids,":[101],"achieved":[103],"efficient":[105],"transduction":[106],"exogenous":[108],"genes":[109],"in":[110],"organoids.":[111],"(4)":[112],"investigated":[114],"suspension":[115],"without":[118],"scaffolds":[119],"easier":[121],"harvesting":[122],"assays.":[124],"These":[125],"techniques":[126],"enable":[127],"us":[128],"develop,":[130],"maintain,":[131],"expand":[133],"quickly":[138],"at":[139],"low":[140],"cost,":[141],"facilitating":[142],"screening":[144],"pathogenic":[146],"factors":[147],"candidate":[149],"treatments":[150],"gastrointestinal":[152],"diseases.":[153]},"counts_by_year":[{"year":2026,"cited_by_count":17},{"year":2025,"cited_by_count":13},{"year":2024,"cited_by_count":11},{"year":2023,"cited_by_count":17},{"year":2022,"cited_by_count":16},{"year":2021,"cited_by_count":18},{"year":2020,"cited_by_count":17},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":13}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2017-12-22T00:00:00"}