{"id":"https://openalex.org/W2768812396","doi":"https://doi.org/10.1094/pdis-09-17-1450-sr","title":"Integrating Local Lesion Assays with Conventional RT-PCR for Detection of Interspecies Tospovirus Reassortants and Mixed Tospovirus Infections","display_name":"Integrating Local Lesion Assays with Conventional RT-PCR for Detection of Interspecies Tospovirus Reassortants and Mixed Tospovirus Infections","publication_year":2017,"publication_date":"2017-11-16","ids":{"openalex":"https://openalex.org/W2768812396","doi":"https://doi.org/10.1094/pdis-09-17-1450-sr","mag":"2768812396","pmid":"https://pubmed.ncbi.nlm.nih.gov/30673408"},"language":"en","primary_location":{"id":"doi:10.1094/pdis-09-17-1450-sr","doi":"https://doi.org/10.1094/pdis-09-17-1450-sr","is_oa":true,"landing_page_url":"https://doi.org/10.1094/pdis-09-17-1450-sr","pdf_url":"https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-17-1450-SR","source":{"id":"https://openalex.org/S135458494","display_name":"Plant Disease","issn_l":"0191-2917","issn":["0191-2917","1943-7692"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":"public-domain","license_id":"https://openalex.org/licenses/public-domain","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Disease","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-17-1450-SR","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5068783562","display_name":"Yaowapa Tantiwanich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I1315830910","display_name":"Ministry of Agriculture and Cooperatives","ror":"https://ror.org/03np0rt96","country_code":"TH","type":"government","lineage":["https://openalex.org/I1315830910"]},{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I198105771","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21","country_code":"TH","type":"education","lineage":["https://openalex.org/I198105771"]},{"id":"https://openalex.org/I4210155113","display_name":"U.S. Horticultural Research Laboratory","ror":"https://ror.org/04a4m2h70","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210122704","https://openalex.org/I4210155113"]},{"id":"https://openalex.org/I4405273255","display_name":"Department of Agriculture","ror":"https://ror.org/05jbwak83","country_code":"TH","type":"government","lineage":["https://openalex.org/I4405273255"]},{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["TH","US"],"is_corresponding":false,"raw_author_name":"Yaowapa Tantiwanich","raw_affiliation_strings":["Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Ministry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand","United States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945","Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350; and Scott Adkins,","Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Min-istry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand; Pissawan Chiemsombat, Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Ministry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand","institution_ids":["https://openalex.org/I1315830910","https://openalex.org/I4405273255"]},{"raw_affiliation_string":"United States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210155113"]},{"raw_affiliation_string":"Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350; and Scott Adkins,","institution_ids":["https://openalex.org/I72951846"]},{"raw_affiliation_string":"Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Min-istry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand; Pissawan Chiemsombat, Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand;","institution_ids":["https://openalex.org/I198105771","https://openalex.org/I4405273255"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013895671","display_name":"Pissawan Chiemsombat","orcid":"https://orcid.org/0000-0002-1670-2184","observed_orcids":["https://orcid.org/0000-0002-1670-2184"]},"institutions":[{"id":"https://openalex.org/I198105771","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21","country_code":"TH","type":"education","lineage":["https://openalex.org/I198105771"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Pissawan Chiemsombat","raw_affiliation_strings":["Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand","institution_ids":["https://openalex.org/I198105771"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065677891","display_name":"R. A. Naidu","orcid":"https://orcid.org/0000-0002-7273-5116","observed_orcids":["https://orcid.org/0000-0002-7273-5116"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I198105771","display_name":"Kasetsart University","ror":"https://ror.org/05gzceg21","country_code":"TH","type":"education","lineage":["https://openalex.org/I198105771"]},{"id":"https://openalex.org/I4210155113","display_name":"U.S. Horticultural Research Laboratory","ror":"https://ror.org/04a4m2h70","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210122704","https://openalex.org/I4210155113"]},{"id":"https://openalex.org/I4405273255","display_name":"Department of Agriculture","ror":"https://ror.org/05jbwak83","country_code":"TH","type":"government","lineage":["https://openalex.org/I4405273255"]},{"id":"https://openalex.org/I72951846","display_name":"Washington State University","ror":"https://ror.org/05dk0ce17","country_code":"US","type":"education","lineage":["https://openalex.org/I72951846"]}],"countries":["TH","US"],"is_corresponding":false,"raw_author_name":"Rayapati A. Naidu","raw_affiliation_strings":["Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350","United States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945","Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350; and Scott Adkins,","Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Min-istry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand; Pissawan Chiemsombat, Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350","institution_ids":["https://openalex.org/I72951846"]},{"raw_affiliation_string":"United States Department of Agriculture-Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210155113"]},{"raw_affiliation_string":"Department of Plant Pathology, Washington State University, Irrigated Agriculture Research and Extension Center, Prosser 99350; and Scott Adkins,","institution_ids":["https://openalex.org/I72951846"]},{"raw_affiliation_string":"Plant Pathology Research Group, Plant Protection Research and Development Office, Department of Agriculture, Min-istry of Agriculture and Cooperatives, Chatuchuk, Bangkok, 10900, Thailand; Pissawan Chiemsombat, Department of Plant Pathology, Faculty of Agriculture at Kamphaengsaen, Kasetsart University, Kamphaengsaen, Nakhon Pathom, 73140, Thailand;","institution_ids":["https://openalex.org/I198105771","https://openalex.org/I4405273255"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027080394","display_name":"Scott T. Adkins","orcid":"https://orcid.org/0000-0003-0095-8414","observed_orcids":["https://orcid.org/0000-0003-0095-8414"]},"institutions":[{"id":"https://openalex.org/I1312222531","display_name":"Agricultural Research Service","ror":"https://ror.org/02d2m2044","country_code":"US","type":"government","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I1336096307","display_name":"United States Department of Agriculture","ror":"https://ror.org/01na82s61","country_code":"US","type":"government","lineage":["https://openalex.org/I1336096307"]},{"id":"https://openalex.org/I4210155113","display_name":"U.S. Horticultural Research Laboratory","ror":"https://ror.org/04a4m2h70","country_code":"US","type":"facility","lineage":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210122704","https://openalex.org/I4210155113"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Scott Adkins","raw_affiliation_strings":["United States Department of Agriculture\u2013Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"United States Department of Agriculture\u2013Agricultural Research Service, U.S. Horticultural Research Laboratory, Fort Pierce, FL 34945","institution_ids":["https://openalex.org/I1312222531","https://openalex.org/I1336096307","https://openalex.org/I4210155113"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":7,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"102","issue":"4","first_page":"715","last_page":"719"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10494","display_name":"Plant Virus Research Studies","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13220","display_name":"Plant and Fungal Interactions Research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11750","display_name":"Phytoplasmas and Hemiptera pathogens","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tospoviruses","display_name":"tospoviruses","score":0.9070000052452087},{"id":"https://openalex.org/keywords/tomato-spotted-wilt-virus","display_name":"tomato spotted wilt virus","score":0.9269999861717224},{"id":"https://openalex.org/keywords/groundnut-ringspot-virus","display_name":"Groundnut ringspot virus","score":0.9950000047683716},{"id":"https://openalex.org/keywords/tomato-chlorotic-spot-virus","display_name":"Tomato chlorotic spot virus","score":1.0},{"id":"https://openalex.org/keywords/viral-coinfection","display_name":"viral coinfection","score":0.7760000228881836},{"id":"https://openalex.org/keywords/virus-detection","display_name":"virus detection","score":0.5109999775886536},{"id":"https://openalex.org/keywords/thrips-vectors","display_name":"thrips vectors","score":0.7409999966621399},{"id":"https://openalex.org/keywords/plant-virus-detection","display_name":"plant virus detection","score":0.3479999899864197}],"concepts":[{"id":"https://openalex.org/C2779553184","wikidata":"https://www.wikidata.org/wiki/Q2716408","display_name":"Tospovirus","level":5,"score":0.9611597061157227},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9059367179870605},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.7823425531387329},{"id":"https://openalex.org/C2779271554","wikidata":"https://www.wikidata.org/wiki/Q29000551","display_name":"Bunyaviridae","level":3,"score":0.7268754839897156},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.5686349868774414},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.5007996559143066},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.4317770004272461},{"id":"https://openalex.org/C109110057","wikidata":"https://www.wikidata.org/wiki/Q1495434","display_name":"Plant virus","level":3,"score":0.39992815256118774},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2611023485660553},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.22653308510780334},{"id":"https://openalex.org/C2910476756","wikidata":"https://www.wikidata.org/wiki/Q1706774","display_name":"Tomato spotted wilt virus","level":4,"score":0.18982627987861633}],"mesh":[{"descriptor_ui":"D010935","descriptor_name":"Plant Diseases","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":true},{"descriptor_ui":"D014026","descriptor_name":"Nicotiana","qualifier_ui":"Q000821","qualifier_name":"virology","is_major_topic":true},{"descriptor_ui":"D016865","descriptor_name":"Reassortant Viruses","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017865","descriptor_name":"Tospovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017865","descriptor_name":"Tospovirus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D020133","descriptor_name":"Reverse Transcriptase Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1094/pdis-09-17-1450-sr","doi":"https://doi.org/10.1094/pdis-09-17-1450-sr","is_oa":true,"landing_page_url":"https://doi.org/10.1094/pdis-09-17-1450-sr","pdf_url":"https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-17-1450-SR","source":{"id":"https://openalex.org/S135458494","display_name":"Plant Disease","issn_l":"0191-2917","issn":["0191-2917","1943-7692"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":"public-domain","license_id":"https://openalex.org/licenses/public-domain","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Disease","raw_type":"journal-article"},{"id":"pmid:30673408","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/30673408","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant disease","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1094/pdis-09-17-1450-sr","doi":"https://doi.org/10.1094/pdis-09-17-1450-sr","is_oa":true,"landing_page_url":"https://doi.org/10.1094/pdis-09-17-1450-sr","pdf_url":"https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-09-17-1450-SR","source":{"id":"https://openalex.org/S135458494","display_name":"Plant Disease","issn_l":"0191-2917","issn":["0191-2917","1943-7692"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320276","host_organization_name":"American Phytopathological Society","host_organization_lineage":["https://openalex.org/P4310320276"],"host_organization_lineage_names":["American Phytopathological Society"],"type":"journal"},"license":"public-domain","license_id":"https://openalex.org/licenses/public-domain","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Plant Disease","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8409000039100647,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7263133586","display_name":null,"funder_award_id":"2012-68004-20166","funder_id":"https://openalex.org/F4320306114","funder_display_name":"U.S. Department of Agriculture"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2768812396.pdf","grobid_xml":"https://content.openalex.org/works/W2768812396.grobid-xml"},"referenced_works_count":34,"referenced_works":["https://openalex.org/W47239484","https://openalex.org/W572223481","https://openalex.org/W1492465290","https://openalex.org/W1968083632","https://openalex.org/W1975316819","https://openalex.org/W1986526676","https://openalex.org/W2000863691","https://openalex.org/W2010556403","https://openalex.org/W2037681442","https://openalex.org/W2037710315","https://openalex.org/W2041118467","https://openalex.org/W2050242597","https://openalex.org/W2057440048","https://openalex.org/W2064499825","https://openalex.org/W2078001317","https://openalex.org/W2080064023","https://openalex.org/W2088993213","https://openalex.org/W2114918273","https://openalex.org/W2118911862","https://openalex.org/W2151528904","https://openalex.org/W2166169320","https://openalex.org/W2167661218","https://openalex.org/W2302356034","https://openalex.org/W2317110807","https://openalex.org/W2340777637","https://openalex.org/W2468629026","https://openalex.org/W2472708491","https://openalex.org/W2548355583","https://openalex.org/W2569504572","https://openalex.org/W2590863492","https://openalex.org/W2594535230","https://openalex.org/W2883445395","https://openalex.org/W2883682896","https://openalex.org/W2884064426"],"related_works":["https://openalex.org/W2063029882","https://openalex.org/W1972188901","https://openalex.org/W2385907218","https://openalex.org/W2063786409","https://openalex.org/W3145064483","https://openalex.org/W2106422891","https://openalex.org/W1967334536","https://openalex.org/W2018235741","https://openalex.org/W2888387516","https://openalex.org/W2783098103"],"abstract_inverted_index":{"Tomato":[0,23],"spotted":[1],"wilt":[2],"virus":[3,20,26,151],"(TSWV)":[4],"has":[5,33],"historically":[6],"been":[7],"the":[8,31,88,98,132,153,190],"major":[9],"tospovirus":[10],"present":[11],"in":[12,28,39,50,97,146,159,174,184,189],"North":[13],"America.":[14],"Recent":[15],"emergence":[16],"of":[17,37,44,71,79,95,131,139,166,180],"Groundnut":[18],"ringspot":[19],"(GRSV)":[21],"and":[22,30,54,65,82,118,135,142,177],"chlorotic":[24],"spot":[25],"(TCSV)":[27],"Florida":[29],"Caribbean":[32],"complicated":[34],"reliable":[35,178],"identification":[36,179],"tospoviruses":[38,47,183],"this":[40,160],"region.":[41],"Field":[42],"symptoms":[43],"these":[45,113,181],"three":[46,182],"are":[48,101],"indistinguishable":[49],"most":[51],"host":[52],"plants,":[53],"commercially":[55],"available":[56],"TSWV":[57],"lateral-flow":[58],"immunoassay":[59],"reagents":[60],"cross":[61],"react":[62],"with":[63,148],"GRSV":[64,72,96],"TCSV,":[66],"leading":[67],"to":[68],"incorrect":[69],"diagnoses":[70],"or":[73,168],"TCSV":[74,83],"as":[75],"TSWV.":[76],"Reliable":[77],"diagnosis":[78],"TSWV,":[80,140],"GRSV,":[81,141],"is":[84],"further":[85],"confounded":[86],"by":[87],"fact":[89],"that":[90],"all":[91],"currently":[92],"known":[93],"isolates":[94],"United":[99],"States":[100],"reassortants":[102,173],"containing":[103],"one":[104],"genomic":[105],"RNA":[106,137],"segment":[107],"derived":[108],"from":[109],"TCSV.":[110,143],"To":[111],"address":[112],"practical":[114],"challenges,":[115],"we":[116],"developed":[117,158],"validated":[119],"genome":[120,154],"segment-specific":[121,155],"primers":[122],"for":[123,171],"conventional":[124],"reverse-transcription":[125],"polymerase":[126],"chain":[127],"reaction":[128],"(RT-PCR)":[129],"detection":[130],"large,":[133],"medium,":[134],"small":[136],"segments":[138],"When":[144],"used":[145],"conjunction":[147],"local":[149],"lesion-passaged":[150],"isolates,":[152],"RT-PCR":[156],"assays":[157],"study":[161],"will":[162],"facilitate":[163],"high-throughput":[164],"screening":[165],"plant":[167],"thrips":[169],"samples":[170],"interspecies":[172],"epidemiological":[175],"studies":[176],"mixed":[185],"infections":[186],"commonly":[187],"observed":[188],"field.":[191]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-12-04T00:00:00"}