{"id":"https://openalex.org/W2761811946","doi":"https://doi.org/10.1007/s10930-017-9744-8","title":"High Yield Preparation of Functionally Active Catalytic-Translocation Domain Module of Botulinum Neurotoxin Type A That Exhibits Uniquely Different Enzyme Kinetics","display_name":"High Yield Preparation of Functionally Active Catalytic-Translocation Domain Module of Botulinum Neurotoxin Type A That Exhibits Uniquely Different Enzyme Kinetics","publication_year":2017,"publication_date":"2017-10-13","ids":{"openalex":"https://openalex.org/W2761811946","doi":"https://doi.org/10.1007/s10930-017-9744-8","mag":"2761811946","pmid":"https://pubmed.ncbi.nlm.nih.gov/29030733","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5705016"},"language":"en","primary_location":{"id":"doi:10.1007/s10930-017-9744-8","doi":"https://doi.org/10.1007/s10930-017-9744-8","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s10930-017-9744-8","pdf_url":null,"source":{"id":"https://openalex.org/S201049572","display_name":"The Protein Journal","issn_l":"1572-3887","issn":["1572-3887","1875-8355"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Protein Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5705016","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021061968","display_name":"Harkiranpreet Kaur Dhaliwal","orcid":"https://orcid.org/0000-0002-1888-6554","observed_orcids":["https://orcid.org/0000-0002-1888-6554"]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]},{"id":"https://openalex.org/I12912129","display_name":"Northeastern University","ror":"https://ror.org/04t5xt781","country_code":"US","type":"education","lineage":["https://openalex.org/I12912129"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Harkiranpreet Kaur Dhaliwal","raw_affiliation_strings":["Biomedical Engineering and Biotechnology Program, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","Department of Pharmaceutical Sciences, Northeastern University, Boston, 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering and Biotechnology Program, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","institution_ids":["https://openalex.org/I100633361"]},{"raw_affiliation_string":"Department of Pharmaceutical Sciences, Northeastern University, Boston, 02115, USA","institution_ids":["https://openalex.org/I12912129"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074780344","display_name":"Nagarajan Thiruvanakarasu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]},{"id":"https://openalex.org/I1320320070","display_name":"United States Food and Drug Administration","ror":"https://ror.org/034xvzb47","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1320320070"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nagarajan Thiruvanakarasu","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","US Food and Drug Administration, Silver Spring, MD, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","institution_ids":["https://openalex.org/I100633361"]},{"raw_affiliation_string":"US Food and Drug Administration, Silver Spring, MD, USA","institution_ids":["https://openalex.org/I1320320070"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007780827","display_name":"Raj Kumar","orcid":"https://orcid.org/0000-0003-2328-8521","observed_orcids":["https://orcid.org/0000-0003-2328-8521","https://orcid.org/0000-0001-7479-696X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Raj Kumar","raw_affiliation_strings":["Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088184767","display_name":"Kruti B. Patel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kruti Patel","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","institution_ids":["https://openalex.org/I100633361"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042220982","display_name":"Ghuncha Ambrin","orcid":"https://orcid.org/0000-0002-9122-2455","observed_orcids":["https://orcid.org/0000-0002-9122-2455"]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ghuncha Ambrin","raw_affiliation_strings":["Biomedical Engineering and Biotechnology Program, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biomedical Engineering and Biotechnology Program, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","institution_ids":["https://openalex.org/I100633361"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087529113","display_name":"Shouwei Cai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I100633361","display_name":"University of Massachusetts Dartmouth","ror":"https://ror.org/00fzmm222","country_code":"US","type":"education","lineage":["https://openalex.org/I100633361"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shouwei Cai","raw_affiliation_strings":["Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, North Dartmouth, MA, 02747, USA","institution_ids":["https://openalex.org/I100633361"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083903249","display_name":"Bal Ram Singh","orcid":"https://orcid.org/0000-0002-9069-1487","observed_orcids":["https://orcid.org/0000-0002-9069-1487"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Bal Ram Singh","raw_affiliation_strings":["Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA. bsingh@inads.org","Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA. bsingh@inads.org","institution_ids":[]},{"raw_affiliation_string":"Botulinum Research Center, Institute of Advanced Sciences, Dartmouth, MA, 02747, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5083903249"],"corresponding_institution_ids":[],"apc_list":{"value":2890,"currency":"EUR","value_usd":3690},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.17685581,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"36","issue":"6","first_page":"489","last_page":"501"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11274","display_name":"Botulinum Toxin and Related Neurological Disorders","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12477","display_name":"Toxin Mechanisms and Immunotoxins","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/botulinum-toxin-type-a","display_name":"botulinum toxin type A","score":0.9890000224113464},{"id":"https://openalex.org/keywords/light-chain","display_name":"light chain","score":0.7250000238418579},{"id":"https://openalex.org/keywords/translocation-domain","display_name":"translocation domain","score":0.9539999961853027},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8679999709129333},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.9549999833106995},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.7509999871253967},{"id":"https://openalex.org/keywords/protein-purification","display_name":"protein purification","score":0.6240000128746033},{"id":"https://openalex.org/keywords/endosomal-translocation","display_name":"endosomal translocation","score":0.5910000205039978},{"id":"https://openalex.org/keywords/substrate-affinity","display_name":"substrate affinity","score":0.41999998688697815}],"concepts":[{"id":"https://openalex.org/C148898269","wikidata":"https://www.wikidata.org/wiki/Q1108792","display_name":"Kinetics","level":2,"score":0.6705623269081116},{"id":"https://openalex.org/C2994342964","wikidata":"https://www.wikidata.org/wiki/Q208413","display_name":"Botulinum neurotoxin","level":3,"score":0.6565225720405579},{"id":"https://openalex.org/C134121241","wikidata":"https://www.wikidata.org/wiki/Q899301","display_name":"Yield (engineering)","level":2,"score":0.5862001776695251},{"id":"https://openalex.org/C56856141","wikidata":"https://www.wikidata.org/wiki/Q883112","display_name":"Enzyme kinetics","level":4,"score":0.5847017168998718},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5689818859100342},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.52838134765625},{"id":"https://openalex.org/C2780358027","wikidata":"https://www.wikidata.org/wiki/Q407752","display_name":"Neurotoxin","level":2,"score":0.5238680243492126},{"id":"https://openalex.org/C138626823","wikidata":"https://www.wikidata.org/wiki/Q916504","display_name":"Chromosomal translocation","level":3,"score":0.45721033215522766},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.42238831520080566},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.4115399122238159},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3673921823501587},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.260913610458374},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22713470458984375},{"id":"https://openalex.org/C2777367657","wikidata":"https://www.wikidata.org/wiki/Q184651","display_name":"Toxin","level":2,"score":0.0835098922252655},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.08084633946418762},{"id":"https://openalex.org/C191897082","wikidata":"https://www.wikidata.org/wiki/Q11467","display_name":"Metallurgy","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072417","descriptor_name":"Protein Domains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D016297","descriptor_name":"Mutagenesis, Site-Directed","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019274","descriptor_name":"Botulinum Toxins, Type A","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1007/s10930-017-9744-8","doi":"https://doi.org/10.1007/s10930-017-9744-8","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s10930-017-9744-8","pdf_url":null,"source":{"id":"https://openalex.org/S201049572","display_name":"The Protein Journal","issn_l":"1572-3887","issn":["1572-3887","1875-8355"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Protein Journal","raw_type":"journal-article"},{"id":"pmid:29030733","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29030733","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The protein journal","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:5232467","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5705016","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5705016","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5705016","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Protein J","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:5232467","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5705016","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4692218304","display_name":"Rapid detection of botulinum toxins using a high sensitivity SPR sensor","funder_award_id":"U01AI078070","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G6204505633","display_name":null,"funder_award_id":"1U01A1078070-02","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W438900549","https://openalex.org/W1487774565","https://openalex.org/W1515102646","https://openalex.org/W1969711536","https://openalex.org/W1973515844","https://openalex.org/W1979084295","https://openalex.org/W1979139649","https://openalex.org/W1980598644","https://openalex.org/W1982185698","https://openalex.org/W1983805250","https://openalex.org/W1984138111","https://openalex.org/W1984235249","https://openalex.org/W1988075499","https://openalex.org/W1989703672","https://openalex.org/W1998965573","https://openalex.org/W2000758651","https://openalex.org/W2000968769","https://openalex.org/W2015098348","https://openalex.org/W2015269687","https://openalex.org/W2018289835","https://openalex.org/W2018321735","https://openalex.org/W2028996235","https://openalex.org/W2033921425","https://openalex.org/W2040326336","https://openalex.org/W2041010702","https://openalex.org/W2070155290","https://openalex.org/W2075826540","https://openalex.org/W2078970687","https://openalex.org/W2091460376","https://openalex.org/W2100837269","https://openalex.org/W2101108802","https://openalex.org/W2117050452","https://openalex.org/W2122977798","https://openalex.org/W2127251085","https://openalex.org/W2130939465","https://openalex.org/W2134886492","https://openalex.org/W2160463128","https://openalex.org/W2163264253"],"related_works":["https://openalex.org/W1979084295","https://openalex.org/W2070155290","https://openalex.org/W1979139649","https://openalex.org/W2137211663","https://openalex.org/W3188850497","https://openalex.org/W228613302","https://openalex.org/W1967680616","https://openalex.org/W2593197492","https://openalex.org/W2006500200","https://openalex.org/W2000968769"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-10-20T00:00:00"}
