{"id":"https://openalex.org/W2761298933","doi":null,"title":"Additive effects among uterine paracrine factors in promoting bovine trophoblast cell proliferation","display_name":"Additive effects among uterine paracrine factors in promoting bovine trophoblast cell proliferation","publication_year":2014,"publication_date":"2014-06-10","ids":{"openalex":"https://openalex.org/W2761298933","mag":"2761298933"},"language":"en","primary_location":{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/79562","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10919/79562","pdf_url":null,"source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047995826","display_name":"Markus Ming Xie","orcid":"https://orcid.org/0000-0003-1350-7364","observed_orcids":["https://orcid.org/0000-0003-1350-7364"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Markus M. Xie","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1350-7364","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5047995826"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11510","display_name":"Reproductive System and Pregnancy","score":0.0017000000225380063,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trophoblast-proliferation","display_name":"trophoblast proliferation","score":0.796999990940094},{"id":"https://openalex.org/keywords/epidermal-growth-factor","display_name":"epidermal growth factor","score":0.9959999918937683},{"id":"https://openalex.org/keywords/fibroblast-growth-factor-2","display_name":"fibroblast growth factor 2","score":0.9959999918937683},{"id":"https://openalex.org/keywords/insulin-like-growth-factor-i","display_name":"insulin-like growth factor I","score":1.0},{"id":"https://openalex.org/keywords/mapk-signaling","display_name":"MAPK signaling","score":0.843999981880188},{"id":"https://openalex.org/keywords/pi3k-akt-signaling","display_name":"PI3K/Akt signaling","score":0.9819999933242798},{"id":"https://openalex.org/keywords/pregnancy-establishment","display_name":"pregnancy establishment","score":0.6549999713897705},{"id":"https://openalex.org/keywords/pd98059","display_name":"PD98059","score":0.753000020980835}],"concepts":[{"id":"https://openalex.org/C7876069","wikidata":"https://www.wikidata.org/wiki/Q14873666","display_name":"Paracrine signalling","level":3,"score":0.7789403200149536},{"id":"https://openalex.org/C2777495146","wikidata":"https://www.wikidata.org/wiki/Q1889778","display_name":"Trophoblast","level":5,"score":0.759379506111145},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.4712424874305725},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4663485884666443},{"id":"https://openalex.org/C62112901","wikidata":"https://www.wikidata.org/wiki/Q189159","display_name":"Cell growth","level":2,"score":0.42596665024757385},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40046313405036926},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3809716999530792},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3154202103614807},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.2535248398780823},{"id":"https://openalex.org/C2779234561","wikidata":"https://www.wikidata.org/wiki/Q11995","display_name":"Pregnancy","level":2,"score":0.15437227487564087},{"id":"https://openalex.org/C2776953305","wikidata":"https://www.wikidata.org/wiki/Q1212935","display_name":"Placenta","level":4,"score":0.1402517557144165},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.12063562870025635},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.08936479687690735},{"id":"https://openalex.org/C172680121","wikidata":"https://www.wikidata.org/wiki/Q26513","display_name":"Fetus","level":3,"score":0.08436211943626404}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:vtechworks.lib.vt.edu:10919/79562","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10919/79562","pdf_url":null,"source":{"id":"https://openalex.org/S4306400248","display_name":"VTechWorks (Virginia Tech)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I859038795","host_organization_name":"Virginia Tech","host_organization_lineage":["https://openalex.org/I859038795"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2761298933","doi":null,"is_oa":false,"landing_page_url":"https://vtechworks.lib.vt.edu/handle/10919/79562","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":186,"referenced_works":["https://openalex.org/W7362341","https://openalex.org/W28002526","https://openalex.org/W57508992","https://openalex.org/W109256707","https://openalex.org/W1501383613","https://openalex.org/W1502488818","https://openalex.org/W1513049769","https://openalex.org/W1513451331","https://openalex.org/W1597459972","https://openalex.org/W1602920058","https://openalex.org/W1633784328","https://openalex.org/W1842329478","https://openalex.org/W1894641937","https://openalex.org/W1934389460","https://openalex.org/W1965831124","https://openalex.org/W1967478020","https://openalex.org/W1967723511","https://openalex.org/W1969704062","https://openalex.org/W1971582979","https://openalex.org/W1971639164","https://openalex.org/W1973990980","https://openalex.org/W1977057042","https://openalex.org/W1977100927","https://openalex.org/W1979549345","https://openalex.org/W1981088384","https://openalex.org/W1985080991","https://openalex.org/W1985288580","https://openalex.org/W1985748545","https://openalex.org/W1986912819","https://openalex.org/W1987803647","https://openalex.org/W1989841936","https://openalex.org/W1990905205","https://openalex.org/W1992913621","https://openalex.org/W1993991418","https://openalex.org/W1994144105","https://openalex.org/W1996476194","https://openalex.org/W1997119504","https://openalex.org/W1998338586","https://openalex.org/W1998683777","https://openalex.org/W2003467497","https://openalex.org/W2004102023","https://openalex.org/W2006499356","https://openalex.org/W2006601239","https://openalex.org/W2007556251","https://openalex.org/W2007634751","https://openalex.org/W2007963940","https://openalex.org/W2011641307","https://openalex.org/W2011850551","https://openalex.org/W2015217968","https://openalex.org/W2015851954","https://openalex.org/W2017411073","https://openalex.org/W2017851662","https://openalex.org/W2018260139","https://openalex.org/W2019890950","https://openalex.org/W2022176169","https://openalex.org/W2024055184","https://openalex.org/W2024508748","https://openalex.org/W2024936891","https://openalex.org/W2025483003","https://openalex.org/W2025505154","https://openalex.org/W2027200579","https://openalex.org/W2028885049","https://openalex.org/W2029362083","https://openalex.org/W2030601224","https://openalex.org/W2033604950","https://openalex.org/W2035563619","https://openalex.org/W2035693752","https://openalex.org/W2036610908","https://openalex.org/W2038507723","https://openalex.org/W2040102422","https://openalex.org/W2043362534","https://openalex.org/W2043582769","https://openalex.org/W2045120449","https://openalex.org/W2048357629","https://openalex.org/W2048720023","https://openalex.org/W2049318191","https://openalex.org/W2049742094","https://openalex.org/W2051006144","https://openalex.org/W2054151200","https://openalex.org/W2054913265","https://openalex.org/W2055011302","https://openalex.org/W2056298924","https://openalex.org/W2056983875","https://openalex.org/W2057245423","https://openalex.org/W2057300185","https://openalex.org/W2058177024","https://openalex.org/W2063334316","https://openalex.org/W2065242378","https://openalex.org/W2065643273","https://openalex.org/W2067381552","https://openalex.org/W2068565307","https://openalex.org/W2068628120","https://openalex.org/W2069375339","https://openalex.org/W2072216957","https://openalex.org/W2073046927","https://openalex.org/W2076812265","https://openalex.org/W2077950681","https://openalex.org/W2078259117","https://openalex.org/W2078408892","https://openalex.org/W2079186956","https://openalex.org/W2085252324","https://openalex.org/W2086424770","https://openalex.org/W2090663991","https://openalex.org/W2092594329","https://openalex.org/W2093269823","https://openalex.org/W2093291689","https://openalex.org/W2096150082","https://openalex.org/W2096421845","https://openalex.org/W2096813946","https://openalex.org/W2097469040","https://openalex.org/W2098274784","https://openalex.org/W2100745995","https://openalex.org/W2101761638","https://openalex.org/W2102319995","https://openalex.org/W2103490973","https://openalex.org/W2105126882","https://openalex.org/W2106342309","https://openalex.org/W2106965515","https://openalex.org/W2108364190","https://openalex.org/W2108662489","https://openalex.org/W2108760842","https://openalex.org/W2110042849","https://openalex.org/W2113103296","https://openalex.org/W2115519682","https://openalex.org/W2116515338","https://openalex.org/W2117674538","https://openalex.org/W2118651279","https://openalex.org/W2118830070","https://openalex.org/W2118890219","https://openalex.org/W2118976848","https://openalex.org/W2119424482","https://openalex.org/W2120580424","https://openalex.org/W2121212588","https://openalex.org/W2122778024","https://openalex.org/W2122892477","https://openalex.org/W2126851387","https://openalex.org/W2129589687","https://openalex.org/W2131074211","https://openalex.org/W2131835832","https://openalex.org/W2132140559","https://openalex.org/W2132673127","https://openalex.org/W2132751927","https://openalex.org/W2133200532","https://openalex.org/W2136081034","https://openalex.org/W2136167435","https://openalex.org/W2136215734","https://openalex.org/W2136600930","https://openalex.org/W2136960342","https://openalex.org/W2138142695","https://openalex.org/W2138178479","https://openalex.org/W2139519097","https://openalex.org/W2139695472","https://openalex.org/W2140122956","https://openalex.org/W2140576646","https://openalex.org/W2141127610","https://openalex.org/W2141269411","https://openalex.org/W2141697501","https://openalex.org/W2147680328","https://openalex.org/W2147995078","https://openalex.org/W2149711164","https://openalex.org/W2150163104","https://openalex.org/W2150295764","https://openalex.org/W2150801318","https://openalex.org/W2151335262","https://openalex.org/W2152613547","https://openalex.org/W2153484787","https://openalex.org/W2153540754","https://openalex.org/W2154472800","https://openalex.org/W2156808865","https://openalex.org/W2157096811","https://openalex.org/W2159400233","https://openalex.org/W2164415361","https://openalex.org/W2165236774","https://openalex.org/W2165655523","https://openalex.org/W2167314607","https://openalex.org/W2168172261","https://openalex.org/W2168583421","https://openalex.org/W2170064419","https://openalex.org/W2170165366","https://openalex.org/W2273436924","https://openalex.org/W2394567496","https://openalex.org/W2409723446","https://openalex.org/W2416838657","https://openalex.org/W2936228358","https://openalex.org/W2938655246","https://openalex.org/W3092083595"],"related_works":["https://openalex.org/W2135089155","https://openalex.org/W2024936891","https://openalex.org/W2017411073","https://openalex.org/W2904744650","https://openalex.org/W1966852842","https://openalex.org/W2073046927","https://openalex.org/W1971618910","https://openalex.org/W2068628120","https://openalex.org/W1989841936","https://openalex.org/W2918803443"],"abstract_inverted_index":{"Several":[0],"uterine-derived":[1,443],"paracrine":[2,212],"factors":[3,21,71,156,170,181,286,321,355,402,444],"have":[4],"been":[5],"implicated":[6],"as":[7,227],"critical":[8],"regulators":[9],"of":[10,32,95,116,166,187,210,259,295,318,333,353,371,380,399,415,425,442,460,485],"conceptus":[11,449],"development":[12,450],"in":[13,104,224,268,279,393,451,462,483],"cattle,":[14],"but":[15,242,302,472],"it":[16],"remains":[17],"unclear":[18],"how":[19,98,193,330],"these":[20,69,211,435,468,486],"work":[22,34],"together":[23],"to":[24,36,61,72,109,191,362,409,430,479],"establish":[25,37],"and":[26,50,60,198,207,216,265,307,336,374,383,418,448,458,474],"maintain":[27],"pregnancies.":[28],"The":[29,92,310,369,464],"primary":[30],"objectives":[31],"this":[33],"were":[35,157,182,287,347,391],"if":[38],"cooperative":[39,175,208,316,440],"interactions":[40,441],"between":[41],"epidermal":[42],"growth":[43,47,52,70,83,169,354,401],"factor":[44,84,100],"(EGF),":[45],"fibroblast":[46],"factor-2":[48],"(FGF2)":[49],"insulin-like":[51],"factor-1":[53],"(IGF1)":[54],"promote":[55,445,455],"bovine":[56,87,339,345],"trophoblast":[57,88,446],"cell":[58,74,89,270,280,324],"proliferation,":[59,301],"decipher":[62],"the":[63,174,205,277,315,331,351,378,397,413,423,456],"intracellular":[64],"signaling":[65,202,476],"mechanisms":[66,465],"employed":[67],"by":[68,127,229],"regulate":[73],"proliferation.":[75,112,271,325],"Pilot":[76],"studies":[77,96,188],"established":[78],"effective":[79],"concentrations":[80],"for":[81,467],"each":[82,99,107],"on":[85,322,403],"a":[86,120,147,256,292],"line":[90],"(CT1).":[91],"first":[93],"set":[94,186],"examined":[97,329],"worked":[101],"individually":[102],"or":[103,136,356],"conjunction":[105],"with":[106,129,142,255,291,350,396,412],"other":[108],"impact":[110,305],"CT1":[111,225,269,300,323],"Mitotic":[113],"index":[114],"(percentage":[115],"EdU-positive":[117],"nuclei":[118],"after":[119],"45":[121],"min":[122],"challenge)":[123],"was":[124,151,189],"increased":[125,243,376,421],"(P<0.05)":[126,150,377,389,422],"supplementation":[128,165],"10":[130,133],"ng/ml":[131,134,138],"EGF,":[132,334,372,416],"FGF2,":[135,335,373,417],"50":[137],"IGF1":[139,217,250,308,337,375,420],"when":[140,153,178,283,407],"compared":[141,408],"non-treated":[143],"controls.":[144,387,410],"In":[145,342,433],"addition,":[146],"greater":[148],"increase":[149],"detected":[152,392],"all":[154,179,284,319],"three":[155,180,285,320,400],"supplemented":[158,288],"together.":[159,289],"A":[160,184,326],"follow-up":[161],"study":[162,328],"determined":[163,228],"that":[164,439,453],"any":[167],"two":[168],"could":[171],"not":[172,237,304],"replicate":[173],"effect":[176],"noted":[177],"provided.":[183],"second":[185],"undertaken":[190],"examine":[192],"mitogen-activated":[194,220],"protein":[195,221],"kinase":[196],"(MAPK)":[197],"phosphoinositide":[199],"3-kinase/AKT":[200],"(PI3K/AKT)":[201],"systems":[203,477],"mediate":[204],"independent":[206],"effects":[209,317],"factors.":[213],"Both":[214],"EGF":[215,248],"transiently":[218],"activated":[219],"kinase3/1":[222],"(MAPK3/1)":[223],"cells":[226],"Western":[230],"Blot":[231],"analysis.":[232],"By":[233],"contrast,":[234],"FGF2":[235],"did":[236,303],"affect":[238,338],"MAPK3/1":[239,260,473],"phosphorylation":[240,245],"status,":[241],"AKT":[244,252,296,311],"status.":[246],"Neither":[247],"nor":[249],"impacted":[251],"activity.":[253],"Supplementation":[254,290],"pharmacological":[257,293],"inhibitor":[258,273,294,312],"(PD98059)":[261],"prevented":[262],"EGF-,":[263],"IGF1-,":[264],"FGF2-dependent":[266],"increases":[267,278],"This":[272],"also":[274,419],"completely":[275],"abolished":[276],"proliferation":[281,447],"observed":[282],"(Wortmannin)":[297],"reduced":[298],"FGF2-stimulated":[299],"EGF-":[306],"effects.":[309],"partially":[313],"attenuated":[314],"final":[327],"combination":[332,352,370,398,414],"embryo":[340],"development.":[341],"vitro":[343,366],"produced":[344],"blastocysts":[346,382,394],"cultured":[348],"either":[349],"vehicle":[357],"only":[358],"from":[359,427],"day":[360,363,404,428],"8":[361,429],"12":[364,405,431],"post-in":[365],"fertilization":[367],"(IVF).":[368],"percentage":[379],"hatched":[381],"outgrowth":[384],"formation":[385],"versus":[386],"Increased":[388],"diameters":[390],"treated":[395],"post-IVF":[406],"Treatment":[411],"change":[424],"diameter":[426],"post-IVF.":[432],"summary,":[434],"observations":[436],"provide":[437],"evidence":[438],"ways":[452],"may":[454],"establishment":[457],"maintenance":[459],"pregnancy":[461],"cattle.":[463],"utilized":[466],"activities":[469],"remain":[470],"unresolved,":[471],"PI3K/AKT":[475],"appear":[478],"play":[480],"integral":[481],"roles":[482],"some":[484],"processes.":[487]},"counts_by_year":[],"updated_date":"2026-09-26T08:19:03.415552","created_date":"2017-10-20T00:00:00"}