{"id":"https://openalex.org/W2755280714","doi":"https://doi.org/10.3389/fimmu.2017.01157","title":"Novel Method for High-Throughput Full-Length IGHV-D-J Sequencing of the Immune Repertoire from Bulk B-Cells with Single-Cell Resolution","display_name":"Novel Method for High-Throughput Full-Length IGHV-D-J Sequencing of the Immune Repertoire from Bulk B-Cells with Single-Cell Resolution","publication_year":2017,"publication_date":"2017-09-14","ids":{"openalex":"https://openalex.org/W2755280714","doi":"https://doi.org/10.3389/fimmu.2017.01157","mag":"2755280714","pmid":"https://pubmed.ncbi.nlm.nih.gov/28959265"},"language":"en","primary_location":{"id":"doi:10.3389/fimmu.2017.01157","doi":"https://doi.org/10.3389/fimmu.2017.01157","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2017.01157","pdf_url":"https://www.frontiersin.org/articles/10.3389/fimmu.2017.01157/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.frontiersin.org/articles/10.3389/fimmu.2017.01157/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014410063","display_name":"Stefano Vergani","orcid":"https://orcid.org/0000-0003-0524-5093","observed_orcids":["https://orcid.org/0000-0003-0524-5093"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]},{"id":"https://openalex.org/I139290212","display_name":"Hofstra University","ror":"https://ror.org/03pm18j10","country_code":"US","type":"education","lineage":["https://openalex.org/I139290212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stefano Vergani","raw_affiliation_strings":["Hofstra-Northwell Health School of Medicine, Hempstead, NY, United States","Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Hofstra-Northwell Health School of Medicine, Hempstead, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I139290212"]},{"raw_affiliation_string":"Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5049625162","display_name":"Ilya Korsunsky","orcid":"https://orcid.org/0000-0003-4848-3948","observed_orcids":["https://orcid.org/0000-0003-4848-3948"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ilya Korsunsky","raw_affiliation_strings":["Robert S. Boas Center for Genomics & Human Genetics, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Robert S. Boas Center for Genomics & Human Genetics, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069452467","display_name":"Andrea Nicola Mazzarello","orcid":"https://orcid.org/0000-0003-2167-8817","observed_orcids":["https://orcid.org/0000-0003-2167-8817"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrea Nicola Mazzarello","raw_affiliation_strings":["Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052838795","display_name":"Gerardo Ferrer","orcid":"https://orcid.org/0000-0002-4084-6815","observed_orcids":["https://orcid.org/0000-0002-4084-6815"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gerardo Ferrer","raw_affiliation_strings":["Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040500242","display_name":"Nicholas Chiorazzi","orcid":"https://orcid.org/0000-0003-1023-6650","observed_orcids":["https://orcid.org/0000-0003-1023-6650"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nicholas Chiorazzi","raw_affiliation_strings":["Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013360540","display_name":"Davide Bagnara","orcid":"https://orcid.org/0000-0001-7889-8103","observed_orcids":["https://orcid.org/0000-0001-7889-8103"]},"institutions":[{"id":"https://openalex.org/I1302444339","display_name":"Northwell Health","ror":"https://ror.org/02bxt4m23","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1302444339"]},{"id":"https://openalex.org/I1327576362","display_name":"Feinstein Institute for Medical Research","ror":"https://ror.org/05dnene97","country_code":"US","type":"facility","lineage":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]},{"id":"https://openalex.org/I83816512","display_name":"University of Genoa","ror":"https://ror.org/0107c5v14","country_code":"IT","type":"education","lineage":["https://openalex.org/I83816512"]}],"countries":["IT","US"],"is_corresponding":true,"raw_author_name":"Davide Bagnara","raw_affiliation_strings":["Department of Experimental Medicine, University of Genoa, Genoa, Italy","Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Experimental Medicine, University of Genoa, Genoa, Italy","institution_ids":["https://openalex.org/I83816512"]},{"raw_affiliation_string":"Karches Centre for Chronic Lymphocytic Leukemia Research, The Feinstein Institute for Medical Research, Northwell Health, Manhasset, NY, United States","institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5013360540"],"corresponding_institution_ids":["https://openalex.org/I1302444339","https://openalex.org/I1327576362","https://openalex.org/I83816512"],"apc_list":{"value":3150,"currency":"CHF","value_usd":3801},"apc_paid":{"value":3150,"currency":"CHF","value_usd":3801},"fwci":1.674,"has_fulltext":true,"cited_by_count":46,"citation_normalized_percentile":{"value":0.83170596,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"8","issue":null,"first_page":"1157","last_page":"1157"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.46950000524520874,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.46950000524520874,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.2167000025510788,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.14830000698566437,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/immunoglobulin-heavy-chain-variable-region","display_name":"immunoglobulin heavy chain variable region","score":0.8259999752044678},{"id":"https://openalex.org/keywords/adaptive-immune-receptor-repertoire","display_name":"adaptive immune receptor repertoire","score":0.8130000233650208},{"id":"https://openalex.org/keywords/b-cell-repertoire","display_name":"B cell receptor repertoire","score":0.5320000052452087},{"id":"https://openalex.org/keywords/single-cell-sequencing","display_name":"single-cell sequencing","score":0.6639999747276306},{"id":"https://openalex.org/keywords/library-preparation","display_name":"library preparation","score":0.7070000171661377},{"id":"https://openalex.org/keywords/unique-molecular-identifiers","display_name":"unique molecular identifiers","score":0.8600000143051147},{"id":"https://openalex.org/keywords/low-input-sequencing","display_name":"low-input sequencing","score":0.47099998593330383},{"id":"https://openalex.org/keywords/immune-diversity","display_name":"immune diversity","score":0.48100000619888306}],"concepts":[{"id":"https://openalex.org/C2777609679","wikidata":"https://www.wikidata.org/wiki/Q5971015","display_name":"IGHV@","level":4,"score":0.7812846899032593},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.6134511828422546},{"id":"https://openalex.org/C2778473898","wikidata":"https://www.wikidata.org/wiki/Q2145110","display_name":"Repertoire","level":2,"score":0.5987725853919983},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5754218697547913},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.5362213850021362},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4678657054901123},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3268853425979614},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.3079450726509094},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.27325665950775146},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.16477733850479126},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.07765433192253113},{"id":"https://openalex.org/C2777938653","wikidata":"https://www.wikidata.org/wiki/Q1088156","display_name":"Chronic lymphocytic leukemia","level":3,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.0},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.3389/fimmu.2017.01157","doi":"https://doi.org/10.3389/fimmu.2017.01157","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2017.01157","pdf_url":"https://www.frontiersin.org/articles/10.3389/fimmu.2017.01157/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},{"id":"pmid:28959265","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28959265","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in immunology","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:4538215","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5603803","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5603803","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5603803","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Front Immunol","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:9f55996cad62435c895bf1abdc69f8df","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/9f55996cad62435c895bf1abdc69f8df","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Frontiers in Immunology, Vol 8 (2017)","raw_type":"article"},{"id":"pmh:oai:iris.unige.it:11567/1039532","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/11567/1039532","pdf_url":null,"source":{"id":"https://openalex.org/S4377196291","display_name":"CINECA IRIS Institutial Research Information System (University of Genoa)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I83816512","host_organization_name":"University of Genoa","host_organization_lineage":["https://openalex.org/I83816512"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.3389/fimmu.2017.01157","doi":"https://doi.org/10.3389/fimmu.2017.01157","is_oa":true,"landing_page_url":"https://doi.org/10.3389/fimmu.2017.01157","pdf_url":"https://www.frontiersin.org/articles/10.3389/fimmu.2017.01157/pdf","source":{"id":"https://openalex.org/S2595292759","display_name":"Frontiers in Immunology","issn_l":"1664-3224","issn":["1664-3224"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320527","host_organization_name":"Frontiers Media","host_organization_lineage":["https://openalex.org/P4310320527"],"host_organization_lineage_names":["Frontiers Media"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Frontiers in Immunology","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.5683000087738037}],"sustainable_development_goals_aurora":[{"id":"https://metadata.un.org/sdg/15","display_name":"Life on land","score":0.47999998927116394}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320322823","display_name":"Fondazione Umberto Veronesi","ror":"https://ror.org/041g7jm83"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2755280714.pdf","grobid_xml":"https://content.openalex.org/works/W2755280714.grobid-xml"},"referenced_works_count":17,"referenced_works":["https://openalex.org/W135571511","https://openalex.org/W1423495995","https://openalex.org/W1516874144","https://openalex.org/W1563668677","https://openalex.org/W1750023785","https://openalex.org/W1782798663","https://openalex.org/W1940255384","https://openalex.org/W2009518556","https://openalex.org/W2051241958","https://openalex.org/W2087704868","https://openalex.org/W2113392525","https://openalex.org/W2151558930","https://openalex.org/W2271544406","https://openalex.org/W2295956658","https://openalex.org/W2310172832","https://openalex.org/W2338760001","https://openalex.org/W2492693765"],"related_works":["https://openalex.org/W2587348978","https://openalex.org/W2951333186","https://openalex.org/W4283363206","https://openalex.org/W2597315323","https://openalex.org/W2170215372","https://openalex.org/W2555584159","https://openalex.org/W1988567016","https://openalex.org/W2119572797","https://openalex.org/W3196202188","https://openalex.org/W2949171194"],"abstract_inverted_index":{"Efficient":[0],"and":[1,22,28,106,143,157,166,188],"accurate":[2],"high-throughput":[3],"DNA":[4],"sequencing":[5,52,56,85],"of":[6,30,38,45,73,104,112,118,134,147,159,194],"the":[7,31,35,46,63,102,108,116,126,132,140,145,153,185],"adaptive":[8],"immune":[9,17],"receptor":[10],"repertoire":[11,92,111],"(AIRR)":[12],"is":[13,98,175,197],"necessary":[14],"to":[15,60],"study":[16],"diversity":[18,29,49],"in":[19,88,121,125,139,178,184],"healthy":[20],"subjects":[21],"disease-related":[23],"conditions.":[24],"The":[25,129],"high":[26],"complexity":[27],"AIRR":[32,55,65],"coupled":[33],"with":[34],"limited":[36,192],"amount":[37,156,193],"starting":[39,127,154,195],"material,":[40],"which":[41],"can":[42],"compromise":[43],"identification":[44],"full":[47],"biological":[48],"makes":[50],"such":[51],"particularly":[53],"challenging.":[54],"protocols":[57],"often":[58],"fail":[59],"fully":[61],"capture":[62],"sampled":[64,96,186],"diversity,":[66],"especially":[67],"for":[68,83,180],"samples":[69],"containing":[70],"restricted":[71],"numbers":[72],"B":[74],"lymphocytes.":[75],"Here,":[76],"we":[77],"describe":[78],"a":[79,113,191],"library":[80],"preparation":[81],"method":[82],"immunoglobulin":[84],"that":[86],"results":[87],"an":[89],"exhaustive":[90],"full-length":[91],"where":[93],"virtually":[94],"every":[95],"B-cell":[97],"sequenced.":[99],"This":[100],"maximizes":[101],"likelihood":[103],"identifying":[105],"quantifying":[107],"entire":[109],"IGHV-D-J":[110],"sample,":[114],"including":[115],"detection":[117],"rearrangements":[119,183],"present":[120],"only":[122,190],"one":[123],"cell":[124],"population.":[128],"methodology":[130,174],"establishes":[131],"importance":[133],"circumventing":[135],"genetic":[136],"material":[137,155,196],"dilution":[138],"preamplification":[141],"phases":[142],"incorporates":[144],"use":[146],"certain":[148],"described":[149],"concepts:":[150],"(1)":[151],"balancing":[152],"depth":[158],"sequencing,":[160],"(2)":[161],"avoiding":[162],"IGHV":[163],"gene-specific":[164],"amplification,":[165],"(3)":[167],"using":[168],"Unique":[169],"Molecular":[170],"Identifier.":[171],"Together,":[172],"this":[173],"highly":[176],"efficient,":[177],"particular":[179],"detecting":[181],"rare":[182],"population":[187],"when":[189],"available.":[198]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":4}],"updated_date":"2026-10-21T09:56:26.102985","created_date":"2025-10-10T00:00:00"}