{"id":"https://openalex.org/W2755055770","doi":"https://doi.org/10.19182/remvt.9353","title":"S\u00e9quence, expression \u00e9lev\u00e9e et purification d\u2019une prot\u00e9ine recombinante de 21 kDa de Cowdria raminantium, \u00e0 partir d\u2019 Escherichia coli","display_name":"S\u00e9quence, expression \u00e9lev\u00e9e et purification d\u2019une prot\u00e9ine recombinante de 21 kDa de Cowdria raminantium, \u00e0 partir d\u2019 Escherichia coli","publication_year":1993,"publication_date":"1993-01-01","ids":{"openalex":"https://openalex.org/W2755055770","doi":"https://doi.org/10.19182/remvt.9353","mag":"2755055770"},"language":"fr","primary_location":{"id":"doi:10.19182/remvt.9353","doi":"https://doi.org/10.19182/remvt.9353","is_oa":true,"landing_page_url":"https://doi.org/10.19182/remvt.9353","pdf_url":"https://revues.cirad.fr/index.php/REMVT/article/download/9353/9347","source":{"id":"https://openalex.org/S2756326209","display_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","issn_l":"0035-1865","issn":["0035-1865","1951-6711"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["ddh","doaj","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310311453","host_organization_name":"French Agricultural Research Centre for International Development","host_organization_lineage":["https://openalex.org/P4310311453"],"host_organization_lineage_names":["French Agricultural Research Centre for International Development"],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://revues.cirad.fr/index.php/REMVT/article/download/9353/9347","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5034751455","display_name":"Anthony F. Barbet","orcid":"https://orcid.org/0000-0002-6891-0380","observed_orcids":["https://orcid.org/0000-0002-6891-0380"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Anthony F. Barbet","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109242959","display_name":"Travis C. McGuire","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Travis McGuire","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5111558844","display_name":"Suman M. Mahan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Suman M. Mahan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.33052277,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"46","issue":"1-2","first_page":"165","last_page":"165"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.8974999785423279,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.8974999785423279,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.027699999511241913,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.010599999688565731,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cowdria-ruminantium","display_name":"Cowdria ruminantium","score":0.9950000047683716},{"id":"https://openalex.org/keywords/cowdriosis","display_name":"cowdriosis","score":0.7319999933242798},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8240000009536743},{"id":"https://openalex.org/keywords/immunodominant-proteins","display_name":"immunodominant proteins","score":0.8700000047683716},{"id":"https://openalex.org/keywords/open-reading-frames","display_name":"open reading frames","score":0.6940000057220459},{"id":"https://openalex.org/keywords/gene-sequencing","display_name":"gene sequencing","score":0.41499999165534973},{"id":"https://openalex.org/keywords/deletion-mapping","display_name":"deletion mapping","score":0.4429999887943268},{"id":"https://openalex.org/keywords/fusion-protein-purification","display_name":"fusion protein purification","score":0.7210000157356262},{"id":"https://openalex.org/keywords/cattle-vaccines","display_name":"cattle vaccines","score":0.367000013589859},{"id":"https://openalex.org/keywords/anaplasma-marginale","display_name":"Anaplasma marginale","score":0.7990000247955322}],"concepts":[{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.8387282490730286},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4869297742843628},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3704811632633209}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.19182/remvt.9353","doi":"https://doi.org/10.19182/remvt.9353","is_oa":true,"landing_page_url":"https://doi.org/10.19182/remvt.9353","pdf_url":"https://revues.cirad.fr/index.php/REMVT/article/download/9353/9347","source":{"id":"https://openalex.org/S2756326209","display_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","issn_l":"0035-1865","issn":["0035-1865","1951-6711"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["ddh","doaj","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310311453","host_organization_name":"French Agricultural Research Centre for International Development","host_organization_lineage":["https://openalex.org/P4310311453"],"host_organization_lineage_names":["French Agricultural Research Centre for International Development"],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:781de200769344e38f15b28b5d6fd878","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/781de200769344e38f15b28b5d6fd878","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Revue d\u2019Elevage et de M\u00e9decine V\u00e9t\u00e9rinaire des Pays Tropicaux, Vol 46, Iss 1-2 (1993)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.19182/remvt.9353","doi":"https://doi.org/10.19182/remvt.9353","is_oa":true,"landing_page_url":"https://doi.org/10.19182/remvt.9353","pdf_url":"https://revues.cirad.fr/index.php/REMVT/article/download/9353/9347","source":{"id":"https://openalex.org/S2756326209","display_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","issn_l":"0035-1865","issn":["0035-1865","1951-6711"],"is_oa":true,"is_in_doaj":true,"is_core":false,"listed_in":["ddh","doaj","norway-1","poland-20","russia-white-list-4"],"host_organization":"https://openalex.org/P4310311453","host_organization_name":"French Agricultural Research Centre for International Development","host_organization_lineage":["https://openalex.org/P4310311453"],"host_organization_lineage_names":["French Agricultural Research Centre for International Development"],"type":"journal"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Revue d\u2019\u00e9levage et de m\u00e9decine v\u00e9t\u00e9rinaire des pays tropicaux","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2755055770.pdf","grobid_xml":"https://content.openalex.org/works/W2755055770.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1967906684","https://openalex.org/W4300820285","https://openalex.org/W2411234697","https://openalex.org/W7097870652","https://openalex.org/W7064959392","https://openalex.org/W2734869413","https://openalex.org/W2382883597","https://openalex.org/W626191367","https://openalex.org/W7098534134","https://openalex.org/W2410167696"],"abstract_inverted_index":{"Un":[0],"des":[1,21,26,120,132,154],"buts":[2],"de":[3,7,15,37,45,67,75,87,90,95,128,139,142,149,171,188,192,195,204,208],"notre":[4],"projet":[5],"est":[6],"fournir":[8],"une":[9,42,201],"source":[10],"commode,":[11],"\u00e9conomique":[12],"et":[13,24,56,61,71,92,104,113,174],"pure":[14],"prot\u00e9ine":[16,43,147,187,206],"pour":[17,119,145,160],"\u00eatre":[18],"test\u00e9e":[19],"dans":[20,25,33,176],"r\u00e9actions":[22],"diagnostiques":[23],"vaccins":[27],"contre":[28],"la":[29,38,108,146,172,205],"cowdriose.":[30],"L'insertion":[31],"d'ADN":[32],"E.":[34],"coli":[35],"recombinante":[36,207],"colonie":[39],"F5.2,":[40],"exprimant":[41],"immunodominante":[44,148],"Cowdria":[46],"ruminantium,":[47],"a":[48,164,183,199],"\u00e9t\u00e9":[49,165,184],"s\u00e9quenc\u00e9e.":[50],"L'ADN":[51],"\u00e9tait":[52],"riche":[53],"en":[54,102,111,169],"A":[55],"T":[57],"(74":[58],"p.":[59],"100),":[60],"hybridait":[62],"avec":[63,74],"tous":[64],"les":[65,114],"isolats":[66],"C.":[68,150,209],"ruminantium":[69,151],"test\u00e9s":[70],"non":[72],"pas":[73],"l'ADN":[76],"bovin":[77],"ou":[78],"d'Anaplasma":[79],"marginale.":[80],"Il":[81],"contient":[82],"deux":[83,115],"longs":[84],"cadres":[85],"ouverts":[86],"lecture":[88],"(COL)":[89],"627":[91,140],"831":[93],"paires":[94,141],"bases.":[96],"Les":[97],"COL":[98,116,138,159],"\u00e9taient":[99],"plus":[100],"riches":[101],"G":[103],"C,":[105],"compar\u00e9s":[106],"\u00e0":[107],"composition":[109],"globale":[110],"bases":[112,143],"codaient":[117],"potentiellement":[118],"prot\u00e9ines":[121],"contenant":[122],"un":[123,177],"peptide":[124,191],"N-terminal.":[125],"Des":[126],"exp\u00e9riences":[127],"d\u00e9l\u00e9tion":[129],"suivies":[130],"par":[131,153,167],"tests":[133],"d'expression":[134,179],"sugg\u00e9raient":[135],"que":[136],"le":[137],"codait":[144],"reconnue":[152],"s\u00e9rums":[155],"d'animaux":[156],"infect\u00e9s.":[157],"Le":[158,190],"ce":[161],"polypeptide":[162],"mature":[163],"amplifi\u00e9":[166],"r\u00e9action":[168],"cha\u00eene":[170],"polym\u00e9rase":[173],"ins\u00e9r\u00e9":[175],"vecteur":[178],"\u00e9lev\u00e9e":[180],"o\u00f9":[181],"il":[182],"exprim\u00e9":[185],"comme":[186],"fusion.":[189],"fusion":[193],"attach\u00e9,":[194],"9":[196],"acides":[197],"amin\u00e9s,":[198],"permis":[200],"purification":[202],"rapide":[203],"ruminantium.":[210]},"counts_by_year":[],"updated_date":"2026-09-06T09:10:53.072986","created_date":"2017-09-25T00:00:00"}