{"id":"https://openalex.org/W2743552389","doi":"https://doi.org/10.1371/journal.pone.0183000","title":"Single cell qPCR reveals that additional HAND2 and microRNA-1 facilitate the early reprogramming progress of seven-factor-induced human myocytes","display_name":"Single cell qPCR reveals that additional HAND2 and microRNA-1 facilitate the early reprogramming progress of seven-factor-induced human myocytes","publication_year":2017,"publication_date":"2017-08-10","ids":{"openalex":"https://openalex.org/W2743552389","doi":"https://doi.org/10.1371/journal.pone.0183000","mag":"2743552389","pmid":"https://pubmed.ncbi.nlm.nih.gov/28796841","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5552090"},"language":"en","primary_location":{"id":"doi:10.1371/journal.pone.0183000","doi":"https://doi.org/10.1371/journal.pone.0183000","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0183000","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183000&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183000&type=printable","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085191104","display_name":"Emre Bektik","orcid":"https://orcid.org/0000-0002-3558-2886","observed_orcids":["https://orcid.org/0000-0002-3558-2886"]},"institutions":[{"id":"https://openalex.org/I1299218025","display_name":"MetroHealth","ror":"https://ror.org/0377srw41","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025"]},{"id":"https://openalex.org/I146399215","display_name":"University of Tsukuba","ror":"https://ror.org/02956yf07","country_code":"JP","type":"education","lineage":["https://openalex.org/I146399215"]},{"id":"https://openalex.org/I4210157264","display_name":"MetroHealth Medical Center","ror":"https://ror.org/05j4p5w63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025","https://openalex.org/I4210157264"]},{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["JP","US"],"is_corresponding":false,"raw_author_name":"Emre Bektik","raw_affiliation_strings":["Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America","School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America","institution_ids":["https://openalex.org/I1299218025","https://openalex.org/I4210157264","https://openalex.org/I58956616"]},{"raw_affiliation_string":"School of Integrative and Global Majors, University of Tsukuba, Tsukuba, Japan","institution_ids":["https://openalex.org/I146399215"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057585429","display_name":"Adrienne T. Dennis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1299218025","display_name":"MetroHealth","ror":"https://ror.org/0377srw41","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025"]},{"id":"https://openalex.org/I4210157264","display_name":"MetroHealth Medical Center","ror":"https://ror.org/05j4p5w63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025","https://openalex.org/I4210157264"]},{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adrienne Dennis","raw_affiliation_strings":["Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America","institution_ids":["https://openalex.org/I1299218025","https://openalex.org/I4210157264","https://openalex.org/I58956616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036343196","display_name":"Prateek Prasanna","orcid":"https://orcid.org/0000-0002-3068-3573","observed_orcids":["https://orcid.org/0000-0002-3068-3573"]},"institutions":[{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Prateek Prasanna","raw_affiliation_strings":["Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, United States of America","institution_ids":["https://openalex.org/I58956616"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027642699","display_name":"Anant Madabhushi","orcid":"https://orcid.org/0000-0002-5741-0399","observed_orcids":["https://orcid.org/0000-0002-5741-0399"]},"institutions":[{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anant Madabhushi","raw_affiliation_strings":["Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, United States of America","institution_ids":["https://openalex.org/I58956616"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005728106","display_name":"Ji\u2010Dong Fu","orcid":"https://orcid.org/0000-0003-2797-5193","observed_orcids":["https://orcid.org/0000-0003-2797-5193"]},"institutions":[{"id":"https://openalex.org/I1299218025","display_name":"MetroHealth","ror":"https://ror.org/0377srw41","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025"]},{"id":"https://openalex.org/I4210157264","display_name":"MetroHealth Medical Center","ror":"https://ror.org/05j4p5w63","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1299218025","https://openalex.org/I4210157264"]},{"id":"https://openalex.org/I58956616","display_name":"Case Western Reserve University","ror":"https://ror.org/051fd9666","country_code":"US","type":"education","lineage":["https://openalex.org/I58956616"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ji-Dong Fu","raw_affiliation_strings":["Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America"],"raw_orcid":"https://orcid.org/0000-0003-2797-5193","affiliations":[{"raw_affiliation_string":"Department of Medicine, Heart and Vascular Research Center, MetroHealth Campus, Case Western Reserve University, Cleveland, Ohio, United States of America","institution_ids":["https://openalex.org/I1299218025","https://openalex.org/I4210157264","https://openalex.org/I58956616"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5005728106"],"corresponding_institution_ids":["https://openalex.org/I1299218025","https://openalex.org/I4210157264","https://openalex.org/I58956616"],"apc_list":{"value":2382,"currency":"USD","value_usd":2382},"apc_paid":{"value":2382,"currency":"USD","value_usd":2382},"fwci":1.0048,"has_fulltext":true,"cited_by_count":19,"citation_normalized_percentile":{"value":0.73685583,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"12","issue":"8","first_page":"e0183000","last_page":"e0183000"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.9603999853134155,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.03739999979734421,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/direct-reprogramming","display_name":"direct reprogramming","score":0.7820000052452087},{"id":"https://openalex.org/keywords/induced-cardiomyocytes","display_name":"induced cardiomyocytes","score":0.8389999866485596},{"id":"https://openalex.org/keywords/cardiac-fibroblasts","display_name":"cardiac fibroblasts","score":0.8619999885559082},{"id":"https://openalex.org/keywords/hand2","display_name":"Hand2","score":0.949999988079071},{"id":"https://openalex.org/keywords/mir-1","display_name":"miR-1","score":0.9929999709129333},{"id":"https://openalex.org/keywords/single-cell-gene-expression","display_name":"single-cell gene expression","score":0.7390000224113464},{"id":"https://openalex.org/keywords/human-embryonic-stem-cell-derived-cardiomyocytes","display_name":"human embryonic stem cell-derived cardiomyocytes","score":0.8659999966621399},{"id":"https://openalex.org/keywords/reprogramming-efficiency","display_name":"reprogramming efficiency","score":0.5260000228881836}],"concepts":[{"id":"https://openalex.org/C77255625","wikidata":"https://www.wikidata.org/wiki/Q402093","display_name":"Reprogramming","level":3,"score":0.9579696655273438},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7356631755828857},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5846098065376282},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5277212858200073},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.4419192671775818},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.4271031618118286},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.3742198944091797},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3415220379829407},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32728856801986694},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.30464035272598267}],"mesh":[{"descriptor_ui":"D000066449","descriptor_name":"Human Embryonic Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D000066449","descriptor_name":"Human Embryonic Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000067470","descriptor_name":"Cellular Reprogramming Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002454","descriptor_name":"Cell Differentiation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D005347","descriptor_name":"Fibroblasts","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D032383","descriptor_name":"Myocytes, Cardiac","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D032383","descriptor_name":"Myocytes, Cardiac","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D035683","descriptor_name":"MicroRNAs","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D051792","descriptor_name":"Basic Helix-Loop-Helix Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D059467","descriptor_name":"Transcriptome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D065150","descriptor_name":"Cellular Reprogramming","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1371/journal.pone.0183000","doi":"https://doi.org/10.1371/journal.pone.0183000","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0183000","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183000&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:5552090","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5552090","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLoS One","raw_type":"Text"},{"id":"pmid:28796841","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28796841","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PloS one","raw_type":"Journal Article"},{"id":"pmh:oai:RePEc:plo:pone00:0183000","doi":null,"is_oa":false,"landing_page_url":"https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0183000","pdf_url":null,"source":{"id":"https://openalex.org/S4306401271","display_name":"RePEc: Research Papers in Economics","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I77793887","host_organization_name":"Federal Reserve Bank of St. Louis","host_organization_lineage":["https://openalex.org/I77793887"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"},{"id":"pmh:oai:doaj.org/article:c3b65f63afd14da6b842eb9480ac2bfd","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/c3b65f63afd14da6b842eb9480ac2bfd","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"PLoS ONE, Vol 12, Iss 8, p e0183000 (2017)","raw_type":"article"},{"id":"pmh:oai:figshare.com:article/5298133","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Single_cell_qPCR_reveals_that_additional_i_HAND2_i_and_microRNA-1_facilitate_the_early_reprogramming_progress_of_seven-factor-induced_human_myocytes/5298133","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"}],"best_oa_location":{"id":"doi:10.1371/journal.pone.0183000","doi":"https://doi.org/10.1371/journal.pone.0183000","is_oa":true,"landing_page_url":"https://doi.org/10.1371/journal.pone.0183000","pdf_url":"https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183000&type=printable","source":{"id":"https://openalex.org/S202381698","display_name":"PLoS ONE","issn_l":"1932-6203","issn":["1932-6203"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","erih-plus","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315706","host_organization_name":"Public Library of Science","host_organization_lineage":["https://openalex.org/P4310315706"],"host_organization_lineage_names":["Public Library of Science"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"PLOS ONE","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9391000270843506}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3258120987","display_name":null,"funder_award_id":"13SDG14580035","funder_id":"https://openalex.org/F4320306230","funder_display_name":"American Heart Association"},{"id":"https://openalex.org/G9000904185","display_name":"Biophysical Modulation of Cardiac Ion Channels by MicroRNA","funder_award_id":"R01HL139006","funder_id":"https://openalex.org/F4320337338","funder_display_name":"National Heart, Lung, and Blood Institute"}],"funders":[{"id":"https://openalex.org/F4320306230","display_name":"American Heart Association","ror":"https://ror.org/013kjyp64"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337338","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2743552389.pdf","grobid_xml":"https://content.openalex.org/works/W2743552389.grobid-xml"},"referenced_works_count":38,"referenced_works":["https://openalex.org/W1976747060","https://openalex.org/W1991340987","https://openalex.org/W1991533533","https://openalex.org/W1994101772","https://openalex.org/W1997806336","https://openalex.org/W2001356598","https://openalex.org/W2003756136","https://openalex.org/W2025899080","https://openalex.org/W2044370692","https://openalex.org/W2058553784","https://openalex.org/W2058950912","https://openalex.org/W2075134815","https://openalex.org/W2076486071","https://openalex.org/W2082714048","https://openalex.org/W2101514652","https://openalex.org/W2105435666","https://openalex.org/W2106586369","https://openalex.org/W2108407164","https://openalex.org/W2108862261","https://openalex.org/W2117431532","https://openalex.org/W2127388725","https://openalex.org/W2136541538","https://openalex.org/W2143690064","https://openalex.org/W2144907960","https://openalex.org/W2149469368","https://openalex.org/W2156140279","https://openalex.org/W2160092129","https://openalex.org/W2166305181","https://openalex.org/W2167202160","https://openalex.org/W2169187183","https://openalex.org/W2173185327","https://openalex.org/W2187089797","https://openalex.org/W2260943715","https://openalex.org/W2290864968","https://openalex.org/W2510157877","https://openalex.org/W2521482126","https://openalex.org/W2554457530","https://openalex.org/W2915142973"],"related_works":["https://openalex.org/W2775570570","https://openalex.org/W3036191479","https://openalex.org/W2108407164","https://openalex.org/W2805979385","https://openalex.org/W2127388725","https://openalex.org/W2395546567","https://openalex.org/W4293568572","https://openalex.org/W2587944271","https://openalex.org/W4383314854","https://openalex.org/W4301016105"],"abstract_inverted_index":{"The":[0,110],"direct":[1,225],"reprogramming":[2,33,37,60,70,120,146,176],"of":[3,80,94,113,143,152,167,175,209,224],"cardiac":[4,189],"fibroblasts":[5,24,40],"into":[6],"induced":[7],"cardiomyocyte":[8],"(CM)-like":[9],"cells":[10,30],"(iCMs)":[11],"holds":[12],"great":[13],"promise":[14],"in":[15,38,50,121,177,192,227],"restoring":[16],"heart":[17],"function.":[18],"We":[19,75],"previously":[20],"found":[21],"that":[22,49,83,158],"human":[23,39,68,100,108,138,212,228],"could":[25,62,163,200],"be":[26],"reprogrammed":[27,128,179],"toward":[28],"CM-like":[29],"by":[31,71,129],"7":[32,130,202],"factors;":[34],"however,":[35],"iCM":[36,69,139],"is":[41],"both":[42],"more":[43,46,178,183],"difficult":[44],"and":[45,87,107,170,198],"time-intensive":[47],"than":[48],"mouse":[51],"cells.":[52,229],"In":[53,195],"this":[54],"study,":[55],"we":[56],"investigated":[57],"if":[58],"additional":[59,144,159],"factors":[61,203],"quantitatively":[63],"and/or":[64],"qualitatively":[65],"improve":[66],"7-factor-mediated":[67],"single-cell":[72,155],"quantitative":[73],"PCR.":[74],"first":[76],"validated":[77],"46":[78,115],"pairs":[79],"TaqMan\u00ae":[81],"primers/probes":[82],"had":[84],"sufficient":[85],"efficiency":[86],"sensitivity":[88],"to":[89,125,204,219],"detect":[90],"the":[91,165,182,186,206],"significant":[92],"difference":[93],"gene":[95],"expression":[96,111],"between":[97],"individual":[98],"H9":[99],"embryonic":[101],"stem":[102],"cell":[103],"(ESC)-differentiated":[104],"CMs":[105],"(H9CMs)":[106],"fibroblasts.":[109,213],"profile":[112],"these":[114],"genes":[116,169,190],"revealed":[117,157],"an":[118],"improved":[119],"12-week":[122],"iCMs":[123,127],"compared":[124],"4-week":[126],"factors,":[131],"indicating":[132],"a":[133,149,172,222],"prolonged":[134],"stochastic":[135],"phase":[136],"during":[137],"reprogramming.":[140],"Although":[141],"none":[142],"one":[145],"factor":[147],"yielded":[148],"greater":[150],"number":[151],"iCMs,":[153],"our":[154],"qPCR":[156],"HAND2":[160,184,197],"or":[161],"microRNA-1":[162,199],"facilitate":[164,205],"silencing":[166],"fibroblast":[168],"yield":[171],"better":[173],"degree":[174],"iCMs.":[180,194],"Noticeably,":[181],"expressed,":[185],"higher-level":[187],"were":[188],"activated":[191],"7Fs+HAND2-reprogrammed":[193],"conclusion,":[196],"help":[201],"early":[207],"progress":[208],"iCM-reprogramming":[210,226],"from":[211],"Our":[214],"study":[215],"provides":[216],"valuable":[217],"information":[218],"further":[220],"optimize":[221],"method":[223]},"counts_by_year":[{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2017-08-17T00:00:00"}