{"id":"https://openalex.org/W2740901754","doi":"https://doi.org/10.1161/str.46.suppl_1.wp388","title":"Abstract W P388: High Fat and High Glucose Synergistically Impair Brain Microvascular Endothelial Cell Survival and Angiogenic Potential After Hypoxia","display_name":"Abstract W P388: High Fat and High Glucose Synergistically Impair Brain Microvascular Endothelial Cell Survival and Angiogenic Potential After Hypoxia","publication_year":2015,"publication_date":"2015-02-01","ids":{"openalex":"https://openalex.org/W2740901754","doi":"https://doi.org/10.1161/str.46.suppl_1.wp388","mag":"2740901754"},"language":"en","primary_location":{"id":"doi:10.1161/str.46.suppl_1.wp388","doi":"https://doi.org/10.1161/str.46.suppl_1.wp388","is_oa":false,"landing_page_url":"https://doi.org/10.1161/str.46.suppl_1.wp388","pdf_url":null,"source":{"id":"https://openalex.org/S62532593","display_name":"Stroke","issn_l":"0039-2499","issn":["0039-2499","1524-4628"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stroke","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103818770","display_name":"John Paul Valenzuela","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"John P Valenzuela","raw_affiliation_strings":["Physiology, Georgia Regents Univ, Augusta, GA","John P Valenzuela"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology, Georgia Regents Univ, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"John P Valenzuela","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065745832","display_name":"Zhi Xiang Qu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zhi Qu","raw_affiliation_strings":["Physiology, Georgia Regents Univ, Augusta, GA","Zhi Qu"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology, Georgia Regents Univ, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Zhi Qu","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100770263","display_name":"Weiguo Li","orcid":"https://orcid.org/0000-0003-3550-018X","observed_orcids":["https://orcid.org/0000-0003-3550-018X"]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Weiguo Li","raw_affiliation_strings":["Physiology, Georgia Regents Univ, Augusta, GA","Weiguo Li"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology, Georgia Regents Univ, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Weiguo Li","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042934677","display_name":"Azza B. El\u2010Remessy","orcid":"https://orcid.org/0000-0003-4386-1989","observed_orcids":["https://orcid.org/0000-0003-4386-1989"]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Azza B El-Remessy","raw_affiliation_strings":["Physiology, Georgia Regents Univ, Augusta, GA","Azza B El-Remessy"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology, Georgia Regents Univ, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Azza B El-Remessy","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033273684","display_name":"Islam Mohamed","orcid":"https://orcid.org/0000-0003-3356-4681","observed_orcids":["https://orcid.org/0000-0003-3356-4681"]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Islam Mohamed","raw_affiliation_strings":["Clinical & Administrative Pharmacy, Univ of Georgia, Augusta, GA","Islam Mohamed"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical & Administrative Pharmacy, Univ of Georgia, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Islam Mohamed","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103352580","display_name":"Susan C. Fagan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan C Fagan","raw_affiliation_strings":["Clinical & Administrative Pharmacy, Univ of Georgia, Augusta, GA","Susan C Fagan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinical & Administrative Pharmacy, Univ of Georgia, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Susan C Fagan","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072730832","display_name":"Adviye Ergul","orcid":"https://orcid.org/0000-0003-0873-6015","observed_orcids":["https://orcid.org/0000-0003-0873-6015"]},"institutions":[{"id":"https://openalex.org/I25041050","display_name":"Augusta University","ror":"https://ror.org/012mef835","country_code":"US","type":"education","lineage":["https://openalex.org/I25041050"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Adviye Ergul","raw_affiliation_strings":["Physiology, Georgia Regents Univ, Augusta, GA","Adviye Ergul"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Physiology, Georgia Regents Univ, Augusta, GA","institution_ids":["https://openalex.org/I25041050"]},{"raw_affiliation_string":"Adviye Ergul","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I25041050"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"46","issue":"suppl_1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.7813000082969666,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11872","display_name":"Barrier Structure and Function Studies","score":0.7813000082969666,"subfield":{"id":"https://openalex.org/subfields/2808","display_name":"Neurology"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.07900000363588333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10227","display_name":"Acute Ischemic Stroke Management","score":0.04019999876618385,"subfield":{"id":"https://openalex.org/subfields/2728","display_name":"Clinical Neurology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/high-fat-diet","display_name":"high-fat diet","score":0.9359999895095825},{"id":"https://openalex.org/keywords/high-glucose","display_name":"high glucose","score":0.8980000019073486},{"id":"https://openalex.org/keywords/brain-microvascular-endothelial-cells","display_name":"brain microvascular endothelial cells","score":0.9399999976158142},{"id":"https://openalex.org/keywords/hypoxia","display_name":"hypoxia","score":0.8069999814033508},{"id":"https://openalex.org/keywords/oxygen-glucose-deprivation","display_name":"oxygen-glucose deprivation","score":0.8349999785423279},{"id":"https://openalex.org/keywords/angiogenesis","display_name":"angiogenesis","score":0.8410000205039978},{"id":"https://openalex.org/keywords/endothelial-cell-survival","display_name":"endothelial cell survival","score":0.9100000262260437},{"id":"https://openalex.org/keywords/palmitate","display_name":"palmitate","score":0.984000027179718},{"id":"https://openalex.org/keywords/free-fatty-acids","display_name":"free fatty acids","score":0.9039999842643738},{"id":"https://openalex.org/keywords/tnf-alpha","display_name":"TNF-\u03b1","score":0.7730000019073486},{"id":"https://openalex.org/keywords/tlr4","display_name":"TLR4","score":0.8320000171661377},{"id":"https://openalex.org/keywords/inflammation","display_name":"inflammation","score":0.781000018119812},{"id":"https://openalex.org/keywords/ischemic-stroke","display_name":"ischemic stroke","score":0.8669999837875366},{"id":"https://openalex.org/keywords/diabetes-mellitus","display_name":"diabetes mellitus","score":0.36500000953674316}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.7798976898193359},{"id":"https://openalex.org/C7836513","wikidata":"https://www.wikidata.org/wiki/Q1641506","display_name":"Hypoxia (environmental)","level":3,"score":0.6812533140182495},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.6508232355117798},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.6122175455093384},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.5764437317848206},{"id":"https://openalex.org/C2780394083","wikidata":"https://www.wikidata.org/wiki/Q539568","display_name":"Angiogenesis","level":2,"score":0.5751499533653259},{"id":"https://openalex.org/C555293320","wikidata":"https://www.wikidata.org/wiki/Q12206","display_name":"Diabetes mellitus","level":2,"score":0.44227445125579834},{"id":"https://openalex.org/C2780972559","wikidata":"https://www.wikidata.org/wiki/Q550061","display_name":"Endothelial dysfunction","level":2,"score":0.42545416951179504},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17775052785873413},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1719343364238739},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.1255303919315338},{"id":"https://openalex.org/C540031477","wikidata":"https://www.wikidata.org/wiki/Q629","display_name":"Oxygen","level":2,"score":0.12384209036827087},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1161/str.46.suppl_1.wp388","doi":"https://doi.org/10.1161/str.46.suppl_1.wp388","is_oa":false,"landing_page_url":"https://doi.org/10.1161/str.46.suppl_1.wp388","pdf_url":null,"source":{"id":"https://openalex.org/S62532593","display_name":"Stroke","issn_l":"0039-2499","issn":["0039-2499","1524-4628"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stroke","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":1.0}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2164611913","https://openalex.org/W4389918488","https://openalex.org/W7098426428","https://openalex.org/W1996980256","https://openalex.org/W2475798720","https://openalex.org/W4396746164","https://openalex.org/W1526336551","https://openalex.org/W3095834082","https://openalex.org/W2367662406","https://openalex.org/W4282013064"],"abstract_inverted_index":{"Diet-induced":[0],"metabolic":[1],"disease":[2],"is":[3],"the":[4,14,21,42,50,94],"most":[5,113],"rapidly":[6,114],"increasing":[7],"risk":[8],"factor":[9],"for":[10,127],"ischemic":[11,39],"stroke":[12],"and":[13,35,55,65,145,151,154,158,176,179,182,199,205,211],"leading":[15],"cause":[16],"of":[17,44],"long-term":[18],"disability":[19],"in":[20,70,82,93,116,197],"United":[22],"States.":[23],"We":[24],"have":[25],"shown":[26,111],"that":[27,52,108,193],"high":[28,53,56,86,95,120],"fat":[29,54,96,121],"diet-induced":[30],"diabetes":[31],"impedes":[32],"vascular":[33],"restoration":[34],"functional":[36],"recovery":[37,209],"after":[38,68,118],"stroke.":[40],"Accordingly,":[41],"objective":[43],"this":[45,219],"study":[46],"was":[47],"to":[48,112,132],"test":[49],"hypothesis":[51],"glucose":[57,84,87,137,198],"combination":[58,167],"impairs":[59],"brain":[60,75],"microvascular":[61,76],"endothelial":[62,77,207],"cell":[63,146,170,208],"survival":[64,142],"angiogenic":[66,148],"potential":[67],"hypoxia":[69,217],"a":[71,103,119,212],"synergistic":[72],"manner.":[73],"Primary":[74],"cells":[78],"(BMVECs)":[79],"were":[80,162],"cultured":[81],"normal":[83],"(Control),":[85],"(HG,":[88],"12.5":[89],"mM,":[90],"levels":[91,184],"observed":[92],"diet-fed":[97],"animals),":[98],"palmitate":[99],"(Pal,":[100],"200":[101],"\u03bcM,":[102],"saturated":[104],"free":[105,200],"fatty":[106,201],"acid":[107],"has":[109],"been":[110],"increase":[115],"plasma":[117],"meal),":[122],"or":[123,135],"HG":[124,164],"plus":[125,165],"Pal":[126,166],"14":[128],"hours":[129],"before":[130],"exposing":[131],"normoxia":[133],"(N)":[134],"oxygen":[136],"deprivation":[138],"(OGD),":[139],"n=4-6.":[140],"Cell":[141],"(MTT":[143],"assay":[144],"counts),":[147],"properties":[149],"(migration":[150],"tube":[152,177],"formation)":[153],"inflammatory":[155],"mediators":[156],"(TNF\u03b1":[157],"toll":[159],"like":[160],"receptor-TLR4)":[161],"measured.":[163],"significantly":[168],"reduced":[169],"number,":[171],"%":[172,174],"viability,":[173],"migration":[175],"length":[178],"increased":[180],"TNF\u03b1":[181],"TLR4":[183],"especially":[185],"under":[186],"OGD":[187],"conditions":[188],"(Table).":[189],"These":[190],"results":[191],"suggest":[192],"even":[194],"mild":[195],"elevations":[196],"acids":[202],"promote":[203],"inflammation":[204],"impair":[206],"mechanisms,":[210],"second":[213],"hit":[214],"such":[215],"as":[216],"exacerbates":[218],"effect.":[220]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2017-08-08T00:00:00"}