{"id":"https://openalex.org/W2740779154","doi":null,"title":"Single-molecule Fluorescence Study on Structural Roles of Guide RNAs in Activation of Cas9 Endonuclease","display_name":"Single-molecule Fluorescence Study on Structural Roles of Guide RNAs in Activation of Cas9 Endonuclease","publication_year":2017,"publication_date":"2017-01-01","ids":{"openalex":"https://openalex.org/W2740779154","mag":"2740779154"},"language":"en","primary_location":{"id":"pmh:oai:s-space.snu.ac.kr:10371/134949","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10371/134949","pdf_url":null,"source":{"id":"https://openalex.org/S4306401345","display_name":"Seoul National University Open Repository (Seoul National University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139264467","host_organization_name":"Seoul National University","host_organization_lineage":["https://openalex.org/I139264467"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"\uc131\uae30\uc6d0","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"\uc131\uae30\uc6d0","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cas9-endonuclease","display_name":"Cas9 endonuclease","score":0.8610000014305115},{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.722000002861023},{"id":"https://openalex.org/keywords/guide-rna","display_name":"guide RNA","score":0.8220000267028809},{"id":"https://openalex.org/keywords/tracrrna","display_name":"tracrRNA","score":0.9359999895095825},{"id":"https://openalex.org/keywords/crrna","display_name":"crRNA","score":0.9390000104904175},{"id":"https://openalex.org/keywords/single-molecule-fluorescence","display_name":"single-molecule fluorescence","score":0.7289999723434448},{"id":"https://openalex.org/keywords/protein-rna-complex","display_name":"protein-RNA complex","score":0.5730000138282776},{"id":"https://openalex.org/keywords/dna-cleavage","display_name":"DNA cleavage","score":0.41600000858306885},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.4699999988079071},{"id":"https://openalex.org/keywords/single-molecule-fret","display_name":"single-molecule FRET","score":0.38199999928474426}],"concepts":[{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.7524313926696777},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.5961413383483887},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5145972967147827},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.43426939845085144},{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.43308913707733154},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4245457351207733},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.415205180644989},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3472139239311218},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2723260819911957},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.27081096172332764},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.25588423013687134},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.12511220574378967},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.05989763140678406},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:s-space.snu.ac.kr:10371/134949","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10371/134949","pdf_url":null,"source":{"id":"https://openalex.org/S4306401345","display_name":"Seoul National University Open Repository (Seoul National University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139264467","host_organization_name":"Seoul National University","host_organization_lineage":["https://openalex.org/I139264467"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2740779154","doi":null,"is_oa":false,"landing_page_url":"https://s-space.snu.ac.kr/bitstream/10371/134949/1/000000141057.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":31,"referenced_works":["https://openalex.org/W638709276","https://openalex.org/W1506494596","https://openalex.org/W1970196890","https://openalex.org/W1996641218","https://openalex.org/W2003171404","https://openalex.org/W2034048377","https://openalex.org/W2045435533","https://openalex.org/W2060177604","https://openalex.org/W2064815984","https://openalex.org/W2066224431","https://openalex.org/W2068280643","https://openalex.org/W2077190448","https://openalex.org/W2094517427","https://openalex.org/W2104820165","https://openalex.org/W2107959600","https://openalex.org/W2108038398","https://openalex.org/W2110058183","https://openalex.org/W2120503144","https://openalex.org/W2122563582","https://openalex.org/W2123746851","https://openalex.org/W2124812694","https://openalex.org/W2135598273","https://openalex.org/W2142515819","https://openalex.org/W2142844453","https://openalex.org/W2149751339","https://openalex.org/W2153344788","https://openalex.org/W2154261378","https://openalex.org/W2163241233","https://openalex.org/W2166139447","https://openalex.org/W2169244146","https://openalex.org/W2237671266"],"related_works":["https://openalex.org/W3163432097","https://openalex.org/W1583429871","https://openalex.org/W6925221084","https://openalex.org/W4243313167","https://openalex.org/W7100848901","https://openalex.org/W6892354429","https://openalex.org/W3209692752","https://openalex.org/W7037144320","https://openalex.org/W3214495034","https://openalex.org/W6912808446"],"abstract_inverted_index":{"\uc6d0\ud575\uc0dd\ubb3c\uc758":[0],"\ud6c4\ucc9c\uba74\uc5ed\uccb4\uacc4\ub85c\uc11c":[1],"\ucd5c\uadfc\ub4e4\uc5b4":[2],"\uc0c8\ub86d\uac8c":[3],"\ubc1c\uacac\ub41c":[4],"\ud06c\ub9ac\uc2a4\ud37c":[5,29],"\uc2dc\uc2a4\ud15c":[6,30,128],"(Clustered":[7],"regularly":[8],"interspaced":[9],"short":[10],"palindromic":[11],"repeats":[12],"(CRISPR)":[13],"/":[14],"CRISPR-associated":[15],"(Cas)":[16],"system)":[17],"\uc740":[18],"\uc678\ubd80\uc758":[19],"\ubc14\uc774\ub7ec\uc2a4\ub098":[20],"\ud50c\ub77c\uc2a4\ubbf8\ub4dc\ub85c\ubd80\ud130":[21],"\uac1c\uccb4":[22],"\uc2a4\uc2a4\ub85c\ub97c":[23],"\ubcf4\ud638\ud558\ub294":[24],"\uae30\ub2a5\uc744":[25],"\uc218\ud589\ud55c\ub2e4.":[26],"\uc5ec\ub7ec":[27],"\ud0c0\uc785\uc758":[28],"\uc911,":[31],"\ud0c0\uc785-II":[32,61],"\uc2dc\uc2a4\ud15c\uc740":[33],"Cas9\uc774\ub77c\ub294":[34],"\ud575\uc0b0\uc808\ub2e8\ud6a8\uc18c":[35,108],"\ub2e8\ubc31\uc9c8\uacfc":[36],"\ub450":[37,104,219,227],"\uac00\uc9c0\uc758":[38],"\uac00\uc774\ub4dc":[39,105],"RNA":[40,43],"(gRNA:":[41],"CRISPR":[42],"(crRNA)":[44],"\uc640":[45],"trans-activating":[46],"crRNA":[47],"(tracrRNA))\ub85c":[48],"\uad6c\uc131\ub418\uba70,":[49],"\uc774":[50,60,138,226],"\uad6c\uc131\uc694\uc18c\ub4e4\uc774":[51],"\ub2e8\ubc31\uc9c8-RNA":[52],"\ubcf5\ud569\uccb4\ub97c":[53],"\uc774\ub8e8\uc5b4":[54],"\ud45c\uc801":[55,188],"DNA":[56,205],"\ub97c":[57],"\uc778\uc2dd\ud558\uace0":[58],"\uc808\ub2e8\ud55c\ub2e4.":[59],"\ud06c\ub9ac\uc2a4\ud37c/Cas9":[62],"\uc2dc\uc2a4\ud15c\uc774":[63],"\ucc28\uc138\ub300":[64],"\uc720\uc804\uc790":[65],"\uc870\uc791":[66],"\uae30\uc220\ub85c":[67],"\uc720\uc804\uacf5\ud559":[68],"\ubd84\uc57c\uc5d0":[69],"\uc131\uacf5\uc801\uc73c\ub85c":[70],"\ub3c4\uc785\ub418\uba74\uc11c,":[71],"Cas9-gRNA":[72,147],"\ubcf5\ud569\uccb4\uc5d0":[73],"\ub300\ud55c":[74,89,93,267],"\uad6c\uc870\uc801":[75,112,262],"\ud2b9\uc131":[76],"\uc5f0\uad6c\ub3c4":[77],"\ud65c\ubc1c\ud788":[78],"\uc9c4\ud589\ub418\uc5c8\ub2e4.":[79],"\uadf8\ub7ec\ub098":[80],"\uc5ec\uc804\ud788":[81],"gRNA\uac00":[82],"Cas9":[83,107,127,159,195,252,260],"\ud575\uc0b0\uc808\ub2e8\ud6a8\uc18c\ub97c":[84],"\uc5b4\ub5bb\uac8c":[85],"\ud65c\uc131\ud654\uc2dc\ud0a4\ub294\uc9c0":[86],"\uadf8":[87,116],"\uad6c\uc870\ubcc0\ud654\uc5d0":[88],"\uad6c\uccb4\uc801\uc778":[90],"\ub3d9\uc5ed\ud559\uc801":[91,233,250],"\uba54\ucee4\ub2c8\uc998\uc5d0":[92],"\uaddc\uba85\uc740":[94],"\uc774\ub8e8\uc5b4\uc9c0\uc9c0":[95,164],"\uc54a\uc740":[96],"\uc2e4\uc815\uc774\ub2e4.\\n":[97],"\uc774\uc5d0,":[98],"\uc6b0\ub9ac\ub294":[99],"\ub2e8\ubd84\uc790":[100],"\ud615\uad11":[101],"\ubd84\uad11\ubc95\uc744":[102],"\ub3c4\uc785\ud558\uc5ec":[103],"RNA\uac00":[106],"\ud65c\uc131\ud654\uc5d0":[109,254,263],"\uc788\uc5b4":[110,151,264],"\uc218\ud589\ud558\ub294":[111],"\uc5ed\ud560\uc5d0":[113,266],"\ub300\ud574":[114,140],"\uc5f0\uad6c\ud558\uc600\ub2e4.":[115],"\uacb0\uacfc,":[117],"\uc815\uc801\uc778":[118],"\uc559\uc0c1\ube14":[119],"\ud3c9\uade0\uac12\ub9cc\uc744":[120],"\uad00\ucc30\ud558\ub294":[121],"\uae30\uc874\uc758":[122],"\uc2e4\ud5d8\ubc95\ub4e4\ub85c\ub294":[123],"\uc54c\uc544\ub0bc":[124],"\uc218":[125,134],"\uc5c6\uc5c8\ub358":[126],"\uc791\ub3d9":[129],"\uc0c1\uc758":[130],"\uc138\ubd80\uc801":[131],"\uba54\ucee4\ub2c8\uc998\uc744":[132],"\uaddc\uba85\ud560":[133],"\uc788\uc5c8\uc73c\uba70,":[135],"\ubcf8":[136],"\ud559\uc704\ub17c\ubb38\uc5d0\uc11c\ub294":[137],"\uc5f0\uad6c\uc5d0":[139],"\ub17c\ud558\uae30\ub85c":[141],"\ud55c\ub2e4.":[142],"\uccab\uc9f8\ub85c,":[143],"tracrRNA\ub294":[144],"\uc815\uc0c1":[145],"\uae30\ub2a5\ud558\ub294":[146],"\ubcf5\ud569\uccb4":[148,193],"(Cas9:gRNA)\uc758":[149],"\ud615\uc131\uacfc\uc815\uc5d0":[150],"\ud575\uc2ec\uc801\uc778":[152],"\uc5ed\ud560\uc744":[153],"\uc218\ud589\ud568\uc744":[154],"\ubc1d\ud600\ub0b4\uc5c8\ub2e4.":[155,256],"tracrRNA\uac00":[156,180],"\uc5c6\ub294":[157],"\uacbd\uc6b0,":[158],"\ub2e8\ubc31\uc9c8\uc774":[160],"\ube44\ud65c\uc131\ud654\ub418\uc5b4":[161],"Cas9:gRNA":[162,181],"\ud615\uc131\uc774":[163],"\uc54a\uc558\ub2e4.":[165,178],"\ubc18\uba74,":[166],"tracrRNA-Cas9":[167],"\uc0c1\ud638\uc791\uc6a9\uc774":[168],"\uac00\ub2a5\ud55c":[169],"\uacbd\uc6b0\uc5d0\ub294":[170],"Cas9:gRNA\uac00":[171,187],"\ud615\uc131\ub418\ub294":[172,207],"\ubc18\uc751\uacbd\ub85c\uac00":[173],"\uc6b0\uc138\ud574\uc9c0\uba74\uc11c":[174],"Cas9\uc758":[175],"\ube44\ud65c\uc131\ud654\ub294":[176],"\uad00\ucc30\ub418\uc9c0":[177],"\ub458\uc9f8\ub85c,":[179],"\ud615\uc131\uc5d0":[182],"\uad00\uc5ec\ud558\ub294":[183],"\uac83\uacfc\ub294":[184],"\ub2ec\ub9ac,":[185],"crRNA\ub294":[186],"DNA\uc640":[189],"\uacb0\ud569\ud55c":[190],"Cas9-gRNA-DNA":[191],"\uc0bc\uc911":[192],"(Cas9:gRNA:DNA)\uc5d0\uc11c":[194],"\ud575\uc0b0\uc808\ub2e8\ud6a8\uc18c\uc758":[196,261],"\ud65c\uc131\ud654":[197],"\uc5ec\ubd80\ub97c":[198],"\uc870\uc808\ud558\ub294":[199],"\uac83\uc73c\ub85c":[200],"\ubc1d\ud600\uc84c\ub2e4.":[201],"Cas9:gRNA:DNA":[202],"\ub0b4\uc758":[203],"crRNA\uc640":[204,247],"\uc0ac\uc774\uc5d0":[206],"R-loop":[208,223],"\uad6c\uc870\ub294":[209,224],"\uc5f4\ub9b0":[210],"\uad6c\uc870":[211,215,249],"(open":[212],"conformation)\uc640":[213],"\uc7a0\uae34":[214,239],"(zipped":[216],"conformation)\ub77c\uace0":[217],"\uba85\uba85\ud55c":[218],"\ud558\uc704":[220],"\uad6c\uc870\ub85c":[221],"\uc874\uc7ac\ud558\uba70,":[222],"\uc2e4\uc81c\uc801\uc73c\ub85c":[225],"\uad6c\uc870\ub97c":[228],"\uacc4\uc18d\ud574\uc11c":[229],"\uc654\ub2e4\uac14\ub2e4":[230],"\ubc18\ubcf5\ud558\ub294":[231],"\ub0b4\uc7ac\uc801\uc778":[232],"\ud2b9\uc131\uc744":[234],"\uc9c0\ub2c8\uace0":[235],"\uc788\uc74c\uc774":[236],"\ubc1c\uacac\ub418\uc5c8\ub2e4.":[237],"\ub098\uc544\uac00,":[238],"\uad6c\uc870\uac00":[240],"\ub9cc\ub4e4\uc5b4\uc838\uc57c\ub9cc":[241],"DNA\uac00":[242],"\uc808\ub2e8\ub41c\ub2e4\ub294":[243],"\uac83\uc744":[244],"\uad00\ucc30\ud568\uc73c\ub85c\uc368,":[245],"\uc774\ub7ec\ud55c":[246,258],"\uc5f0\uad00\ub41c":[248],"\ud2b9\uc131\uc774":[251],"\uc808\ub2e8\ud6a8\uc18c":[253],"\ud575\uc2ec\uc784\uc744":[255],"\uc885\ud569\ud558\uc790\uba74,":[257],"\uacb0\uacfc\ub4e4\uc740":[259],"gRNA\uc758":[265],"\uc138\ubd80":[268],"\uba54\ucee4\ub2c8\uc998":[269],"\uc218\uc900\uc5d0\uc11c\uc758":[270],"\uc774\ud574\ub97c":[271],"\uac00\ub2a5\ucf00":[272],"\ud558\uc600\ub2e4.":[273]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2017-08-08T00:00:00"}