{"id":"https://openalex.org/W273620554","doi":null,"title":"Development of principle culture medium formulations for Saccharomyces cerevisiae","display_name":"Development of principle culture medium formulations for Saccharomyces cerevisiae","publication_year":2014,"publication_date":"2014-09-01","ids":{"openalex":"https://openalex.org/W273620554","mag":"273620554"},"language":"en","primary_location":{"id":"pmh:oai:run.unl.pt:10362/14148","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/14148","pdf_url":"https://run.unl.pt/bitstreams/614a49c2-2c2d-4f4a-aeb0-3ea76ddc6521/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://run.unl.pt/bitstreams/614a49c2-2c2d-4f4a-aeb0-3ea76ddc6521/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021630822","display_name":"Valter Renato Silva Frade","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Valter Renato Silva Frade","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5021630822"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9990000128746033},{"id":"https://openalex.org/keywords/defined-culture-medium","display_name":"defined culture medium","score":0.6710000038146973},{"id":"https://openalex.org/keywords/medium-formulation","display_name":"medium formulation","score":0.6330000162124634},{"id":"https://openalex.org/keywords/elementary-flux-modes","display_name":"elementary flux modes","score":0.9589999914169312},{"id":"https://openalex.org/keywords/rational-design","display_name":"rational design","score":0.5220000147819519},{"id":"https://openalex.org/keywords/metabolic-networks","display_name":"metabolic networks","score":0.6039999723434448},{"id":"https://openalex.org/keywords/amino-acid-supplementation","display_name":"amino acid supplementation","score":0.7490000128746033},{"id":"https://openalex.org/keywords/biomass-production","display_name":"biomass production","score":0.6050000190734863},{"id":"https://openalex.org/keywords/cellular-respiration","display_name":"cellular respiration","score":0.796999990940094},{"id":"https://openalex.org/keywords/glucose-utilization","display_name":"glucose utilization","score":0.7179999947547913},{"id":"https://openalex.org/keywords/batch-to-batch-variability","display_name":"batch-to-batch variation","score":0.7480000257492065},{"id":"https://openalex.org/keywords/culture-medium-optimization","display_name":"culture medium optimization","score":0.36000001430511475},{"id":"https://openalex.org/keywords/co2-production","display_name":"CO2 production","score":0.44999998807907104},{"id":"https://openalex.org/keywords/metabolic-flux-analysis","display_name":"metabolic flux analysis","score":0.33899998664855957}],"concepts":[{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.8449125289916992},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.5080671310424805},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.39213109016418457},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.35787078738212585},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.2782675325870514},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.25536978244781494},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.20291230082511902},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18360808491706848}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:run.unl.pt:10362/14148","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/14148","pdf_url":"https://run.unl.pt/bitstreams/614a49c2-2c2d-4f4a-aeb0-3ea76ddc6521/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},{"id":"mag:273620554","doi":null,"is_oa":false,"landing_page_url":"https://run.unl.pt/bitstream/10362/14148/1/Frade_2014.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:run.unl.pt:10362/14148","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/10362/14148","pdf_url":"https://run.unl.pt/bitstreams/614a49c2-2c2d-4f4a-aeb0-3ea76ddc6521/download","source":{"id":"https://openalex.org/S4306402433","display_name":"Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"master thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W273620554.pdf","grobid_xml":"https://content.openalex.org/works/W273620554.grobid-xml"},"referenced_works_count":60,"referenced_works":["https://openalex.org/W574008402","https://openalex.org/W1487841058","https://openalex.org/W1529898704","https://openalex.org/W1579228577","https://openalex.org/W1758395617","https://openalex.org/W1964491374","https://openalex.org/W1969583812","https://openalex.org/W1979183203","https://openalex.org/W1981096637","https://openalex.org/W1984378303","https://openalex.org/W1985517408","https://openalex.org/W1986641657","https://openalex.org/W1989965628","https://openalex.org/W1992322276","https://openalex.org/W1995044503","https://openalex.org/W1997686890","https://openalex.org/W1998977949","https://openalex.org/W1999640817","https://openalex.org/W2003835605","https://openalex.org/W2010443078","https://openalex.org/W2017422800","https://openalex.org/W2023825654","https://openalex.org/W2026089579","https://openalex.org/W2028232771","https://openalex.org/W2031210162","https://openalex.org/W2039569973","https://openalex.org/W2041117835","https://openalex.org/W2046760236","https://openalex.org/W2049642322","https://openalex.org/W2063475537","https://openalex.org/W2067025578","https://openalex.org/W2072096798","https://openalex.org/W2078071144","https://openalex.org/W2086429585","https://openalex.org/W2088422134","https://openalex.org/W2091497745","https://openalex.org/W2091682884","https://openalex.org/W2091793452","https://openalex.org/W2094399313","https://openalex.org/W2095197013","https://openalex.org/W2105406757","https://openalex.org/W2107792714","https://openalex.org/W2110355460","https://openalex.org/W2114371670","https://openalex.org/W2116324021","https://openalex.org/W2120714068","https://openalex.org/W2122638857","https://openalex.org/W2132617512","https://openalex.org/W2133826923","https://openalex.org/W2134994535","https://openalex.org/W2140670503","https://openalex.org/W2143861551","https://openalex.org/W2144372567","https://openalex.org/W2155829013","https://openalex.org/W2162480880","https://openalex.org/W2163823651","https://openalex.org/W2168153938","https://openalex.org/W2169913476","https://openalex.org/W2170254146","https://openalex.org/W2170739315"],"related_works":["https://openalex.org/W4409122390","https://openalex.org/W4247008839","https://openalex.org/W2272884472","https://openalex.org/W2120893682","https://openalex.org/W3189700398","https://openalex.org/W2368613590","https://openalex.org/W4407803692","https://openalex.org/W2524067501","https://openalex.org/W7096670507","https://openalex.org/W4382631567"],"abstract_inverted_index":{"Saccharomyces":[0,189,206,247],"cerevisiae":[1,190,207,248],"as":[2,4,179],"well":[3],"other":[5,374],"microorganisms":[6],"are":[7,61,128],"frequently":[8],"used":[9,238],"in":[10,25,226,239,402],"industry":[11],"with":[12,106,140,163],"the":[13,40,53,56,69,84,123,143,155,175,243,262,287,315,322,325,335,344,350,354,367,370,373,389,392,396],"purpose":[14],"of":[15,19,55,72,87,116,145,160,205,220,309,324,357,372],"obtain":[16,36,137],"different":[17,147],"kind":[18,115],"products":[20],"that":[21,127,172,223],"can":[22,173],"be":[23,151],"applied":[24],"several":[26,212],"areas":[27],"(research":[28],"investigation,":[29],"pharmaceutical":[30],"compounds,":[31],"etc.).":[32],"In":[33,134,182,198],"order":[34,135,240],"to":[35,46,82,91,95,102,136,150,168,241],"high":[37,125,177],"yields":[38,60,126,178],"for":[39,188,245,334,343,388,413],"desired":[41],"product,":[42],"it":[43,79,165],"is":[44,75,80,166,208],"necessary":[45],"make":[47],"an":[48],"adequate":[49],"medium":[50,187,375],"supplementation":[51],"during":[52],"growth":[54],"microorganisms.":[57],"The":[58,234,307,399],"higher":[59],"typically":[62],"reached":[63],"by":[64,130,314,379,386],"using":[65,131,193],"complex":[66,88,132,180],"media,":[67,89,105,117],"however":[68],"exact":[70,85],"formulation":[71],"these":[73,114],"media":[74,142,171,414],"not":[76,121,215],"known.":[77],"Moreover,":[78],"difficult":[81],"control":[83],"composition":[86],"leading":[90],"batch-to-batch":[92],"variations.":[93],"So,":[94],"overcome":[96],"this":[97,183,199,403],"problem,":[98],"some":[99],"industries":[100,156],"choose":[101],"use":[103,157],"defined":[104,108,141,170,186],"a":[107,158,185,194,201,211,381,407],"and":[109,214,270,278,294,385,395,409],"known":[110],"chemical":[111],"composition.":[112],"However":[113],"many":[118,146],"times,":[119],"do":[120],"reach":[122,174],"same":[124,176],"obtained":[129],"media.":[133,181],"similar":[138],"yield":[139],"addition":[144],"compounds":[148,376,393],"has":[149],"tested":[152],"experimentally.":[153],"Therefore,":[154],"set":[159],"empirical":[161],"methods":[162],"which":[164],"tried":[167],"formulate":[169],"thesis,":[184],"was":[191,237],"developed":[192],"rational":[195],"design":[196],"approach.":[197],"approach":[200,400],"given":[202],"metabolic":[203,221,249,254,259,264,352,359],"network":[204,265,289,327,353],"divided":[209,272,296],"into":[210,363],"unique":[213],"further":[216],"decomposable":[217],"sub":[218],"networks":[219,250],"reactions":[222],"work":[224],"coherently":[225],"steady":[227],"state,":[228],"so":[229],"called":[230],"elementary":[231],"flux":[232],"modes.":[233],"EFMtool":[235],"algorithm":[236],"calculate":[242],"EFM\u2019s":[244,267,275,280,291,299,303,308,346,356],"two":[246],"(amino":[251],"acids":[252,257],"supplemented":[253,263,326],"network;":[255],"amino":[256],"non-supplemented":[258,288,351],"network).":[260],"For":[261,286,349],"1352172":[266],"were":[268,292,312,328,361,377],"calculated":[269,293,378],"then":[271,295],"into:":[273,297,331],"1306854":[274,336],"producing":[276,282,300,304,338,347],"biomass,":[277],"18582":[279,345],"exclusively":[281,305],"CO2":[283],"(cellular":[284],"respiration).":[285],"635":[290],"215":[298],"biomass;":[301],"420":[302],"CO2.":[306,348],"each":[310,358],"group":[311],"normalized":[313],"respective":[316,355],"glucose":[317,383,397],"consumption":[318],"value.":[319],"After":[320,366],"that,":[321],"EFMs\u2019":[323],"grouped":[329,362],"again":[330],"30":[332],"clusters":[333,342],"EFMs":[337],"biomass":[339],"and,":[340],"20":[341],"function":[360],"10":[364],"clusters.":[365],"clustering":[368],"step,":[369],"concentrations":[371,394],"considering":[380],"reasonable":[382],"amount":[384],"accounting":[387],"proportionality":[390],"between":[391],"ratios.":[398],"adopted/developed":[401],"thesis":[404],"may":[405],"allow":[406],"faster":[408],"more":[410],"economical":[411],"way":[412],"development.":[415]},"counts_by_year":[],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2016-06-24T00:00:00"}