{"id":"https://openalex.org/W2731862985","doi":null,"title":"Characterizing Structural Changes of the Magnesium channel, CorA: Implications for Gating","display_name":"Characterizing Structural Changes of the Magnesium channel, CorA: Implications for Gating","publication_year":2015,"publication_date":"2015-11-01","ids":{"openalex":"https://openalex.org/W2731862985","mag":"2731862985"},"language":"en","primary_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/71025","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/71025","pdf_url":"https://utoronto.scholaris.ca/bitstreams/01316cb2-4188-466f-b0d0-fccbfa612f87/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://utoronto.scholaris.ca/bitstreams/01316cb2-4188-466f-b0d0-fccbfa612f87/download","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040836846","display_name":"Angela S. Li","orcid":"https://orcid.org/0000-0002-0397-2193","observed_orcids":["https://orcid.org/0000-0002-0397-2193"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Angela Shaoxu Li","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5040836846"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.9839000105857849,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0038999998942017555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12280","display_name":"Magnesium in Health and Disease","score":0.003100000089034438,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cora","display_name":"Cora","score":0.968999981880188},{"id":"https://openalex.org/keywords/magnesium-channel","display_name":"magnesium channel","score":0.9309999942779541},{"id":"https://openalex.org/keywords/channel-gating","display_name":"channel gating","score":0.8410000205039978},{"id":"https://openalex.org/keywords/salt-bridges","display_name":"salt bridges","score":0.6589999794960022},{"id":"https://openalex.org/keywords/magnesium-binding","display_name":"magnesium binding","score":0.8360000252723694},{"id":"https://openalex.org/keywords/conformational-change","display_name":"conformational change","score":0.671999990940094},{"id":"https://openalex.org/keywords/limited-proteolysis","display_name":"limited proteolysis","score":0.8370000123977661},{"id":"https://openalex.org/keywords/cysteine-cross-linking","display_name":"cysteine cross-linking","score":0.9890000224113464}],"concepts":[{"id":"https://openalex.org/C194544171","wikidata":"https://www.wikidata.org/wiki/Q21105679","display_name":"Gating","level":2,"score":0.6740083694458008},{"id":"https://openalex.org/C543218039","wikidata":"https://www.wikidata.org/wiki/Q660","display_name":"Magnesium","level":2,"score":0.5816971659660339},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3398708701133728},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.24842217564582825},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.09857240319252014},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:utoronto.scholaris.ca:1807/71025","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/71025","pdf_url":"https://utoronto.scholaris.ca/bitstreams/01316cb2-4188-466f-b0d0-fccbfa612f87/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},{"id":"mag:2731862985","doi":null,"is_oa":false,"landing_page_url":"https://tspace.library.utoronto.ca/handle/1807/71025?mode=full","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:utoronto.scholaris.ca:1807/71025","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/1807/71025","pdf_url":"https://utoronto.scholaris.ca/bitstreams/01316cb2-4188-466f-b0d0-fccbfa612f87/download","source":{"id":"https://openalex.org/S4306400261","display_name":"TSpace (University of Toronto)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I185261750","host_organization_name":"University of Toronto","host_organization_lineage":["https://openalex.org/I185261750"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2731862985.pdf","grobid_xml":"https://content.openalex.org/works/W2731862985.grobid-xml"},"referenced_works_count":19,"referenced_works":["https://openalex.org/W1499345269","https://openalex.org/W1537110470","https://openalex.org/W1564624016","https://openalex.org/W1629869967","https://openalex.org/W1969384291","https://openalex.org/W2021663901","https://openalex.org/W2029020115","https://openalex.org/W2076369168","https://openalex.org/W2101937063","https://openalex.org/W2102521379","https://openalex.org/W2106287785","https://openalex.org/W2107845498","https://openalex.org/W2114431583","https://openalex.org/W2118129275","https://openalex.org/W2123772311","https://openalex.org/W2126810236","https://openalex.org/W2141673637","https://openalex.org/W2149601578","https://openalex.org/W2412410059"],"related_works":["https://openalex.org/W2092002507","https://openalex.org/W2284274913","https://openalex.org/W2989816529","https://openalex.org/W4319933708","https://openalex.org/W2253702229","https://openalex.org/W2476568427","https://openalex.org/W4295269614","https://openalex.org/W2117563536","https://openalex.org/W7129263531","https://openalex.org/W2584962655"],"abstract_inverted_index":{"CorA":[0,33,73,81,149,225],"is":[1,199],"the":[2,9,15,29,68,99,106,115,122,132,143,148,163,169,175,182,187,191,208,218,221,229,236,241,244,248,261],"primary":[3],"magnesium":[4,19],"channel":[5,133,211,262],"in":[6,72,80,120,152,235,259],"prokaryotes":[7],"and":[8,34,48,61,88,126,240],"most":[10],"extensively":[11],"studied":[12],"member":[13],"of":[14,18,32,46,59,101,114,147,162,190,220,224,243],"CorA/Mrs2/Alr1":[16],"superfamily":[17],"channels":[20],"that":[21,98,131,174],"extends":[22],"into":[23],"eukaryotes.":[24],"Existing":[25],"crystallographic":[26],"structures":[27],"define":[28],"closed":[30,171],"state":[31],"provide":[35],"a":[36,57,111,153,255],"structural":[37,77,145,233],"basis":[38,223],"for":[39],"its":[40,135],"structure-function":[41],"relationships.":[42],"However,":[43],"detailed":[44],"mechanisms":[45],"gating":[47,82,257],"ion":[49],"permeation":[50],"remain":[51],"poorly":[52],"understood.":[53],"In":[54],"this":[55,203,251],"thesis,":[56],"combination":[58],"biochemical":[60],"biophysical":[62],"methods":[63],"were":[64,83],"used":[65],"to":[66,168,185],"understand":[67],"molecular":[69,222],"details":[70],"involved":[71,79],"gating.":[74,139,212],"Specifically,":[75],"dynamic":[76],"elements":[78],"identified":[84],"through":[85],"limited":[86,124],"proteolysis":[87,125],"mass":[89,127],"spectrometry.":[90],"Cysteine":[91],"cross-linking":[92],"along":[93],"with":[94,121,254],"mutagenesis":[95],"assays":[96],"show":[97],"formation":[100],"an":[102],"inter-protomer":[103],"salt-bridge":[104],"at":[105],"membrane-cytosol":[107,136],"interface":[108,137],"may":[109,206],"be":[110],"key":[112],"component":[113],"activation":[116],"mechanism.":[117],"This":[118],"ties":[119],"earlier":[123],"spectrometry":[128],"results":[129,249],"indicating":[130],"exposes":[134],"during":[138],"Finally,":[140],"we":[141],"report":[142],"first":[144],"elucidation":[146],"soluble":[150,164,183,237],"domain":[151,165,177,184,239],"pentameric":[154],"arrangement.":[155],"The":[156],"structure":[157],"reveals":[158],"significantly":[159],"increased":[160],"closure":[161],"funnel":[166,192],"compared":[167],"regular":[170],"state,":[172],"suggesting":[173],"membrane":[176,245],"can":[178],"impose":[179],"constraints":[180],"on":[181,202,228],"resist":[186],"closing":[188],"motion":[189,242],"triggered":[193],"by":[194],"Mg2+":[195],"binding.":[196],"A":[197],"model":[198],"proposed":[200],"based":[201],"observation,":[204],"which":[205,260],"explain":[207],"mechanics":[209],"underlying":[210],"Altogether,":[213],"these":[214],"studies":[215],"have":[216],"furthered":[217],"understanding":[219],"gating,":[226],"particularly":[227],"communication":[230],"mechanism":[231,258],"linking":[232],"rearrangements":[234],"regulatory":[238],"domain.":[246],"Furthermore,":[247],"from":[250],"thesis":[252],"agree":[253],"sequential":[256],"undergoes":[263],"asymmetric":[264],"conformational":[265],"changes.":[266]},"counts_by_year":[],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2017-07-14T00:00:00"}