{"id":"https://openalex.org/W269634592","doi":null,"title":"Manipulating posttranslational modification in natural product biosynthesis","display_name":"Manipulating posttranslational modification in natural product biosynthesis","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W269634592","mag":"269634592"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt0sn8v44w","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/0sn8v44w","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082178342","display_name":"Timothy L. Foley","orcid":"https://orcid.org/0000-0002-5987-5253","observed_orcids":["https://orcid.org/0000-0002-5987-5253"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Timothy L. Foley","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5987-5253","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5082178342"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.9794999957084656,"subfield":{"id":"https://openalex.org/subfields/2402","display_name":"Applied Microbiology and Biotechnology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11440","display_name":"Click Chemistry and Applications","score":0.0010000000474974513,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/post-translational-modification","display_name":"post-translational modification","score":0.9570000171661377},{"id":"https://openalex.org/keywords/phosphopantetheinyl-transferase","display_name":"phosphopantetheinyl transferase","score":0.8399999737739563},{"id":"https://openalex.org/keywords/fatty-acid-synthase","display_name":"fatty acid synthase","score":0.6539999842643738},{"id":"https://openalex.org/keywords/polyketide-synthase","display_name":"polyketide synthase","score":0.9829999804496765},{"id":"https://openalex.org/keywords/nonribosomal-peptide-synthetase","display_name":"nonribosomal peptide synthetase","score":0.9490000009536743},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.6140000224113464}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6749367713928223},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.6290990114212036},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6198279857635498},{"id":"https://openalex.org/C2779123261","wikidata":"https://www.wikidata.org/wiki/Q407635","display_name":"Coenzyme A","level":4,"score":0.6029582619667053},{"id":"https://openalex.org/C2777379556","wikidata":"https://www.wikidata.org/wiki/Q1443801","display_name":"Nonribosomal peptide","level":4,"score":0.5730398893356323},{"id":"https://openalex.org/C2779396153","wikidata":"https://www.wikidata.org/wiki/Q901227","display_name":"Natural product","level":2,"score":0.4856802225112915},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.47606366872787476},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3740924000740051},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3538297414779663},{"id":"https://openalex.org/C134651460","wikidata":"https://www.wikidata.org/wiki/Q3100717","display_name":"Reductase","level":3,"score":0.09545823931694031}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt0sn8v44w","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/0sn8v44w","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:269634592","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt0sn8v44w/qt0sn8v44w.pdf?t=ml35w0","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":166,"referenced_works":["https://openalex.org/W1494581922","https://openalex.org/W1514973939","https://openalex.org/W1518071796","https://openalex.org/W1529188376","https://openalex.org/W1543635827","https://openalex.org/W1546422804","https://openalex.org/W1547779977","https://openalex.org/W1552834733","https://openalex.org/W1557513215","https://openalex.org/W1565067506","https://openalex.org/W1569049012","https://openalex.org/W1574437110","https://openalex.org/W1593550392","https://openalex.org/W1603339657","https://openalex.org/W1640748652","https://openalex.org/W1875675415","https://openalex.org/W1887414277","https://openalex.org/W1892570967","https://openalex.org/W1907713996","https://openalex.org/W1916437228","https://openalex.org/W1921854009","https://openalex.org/W1964141703","https://openalex.org/W1964789691","https://openalex.org/W1964812851","https://openalex.org/W1964952712","https://openalex.org/W1965085059","https://openalex.org/W1968222033","https://openalex.org/W1968261923","https://openalex.org/W1968839115","https://openalex.org/W1968870211","https://openalex.org/W1974146776","https://openalex.org/W1975604260","https://openalex.org/W1977704501","https://openalex.org/W1979700983","https://openalex.org/W1980530950","https://openalex.org/W1981565802","https://openalex.org/W1981659539","https://openalex.org/W1985328966","https://openalex.org/W1985888380","https://openalex.org/W1986461283","https://openalex.org/W1989281113","https://openalex.org/W1991001144","https://openalex.org/W1994454916","https://openalex.org/W1994477951","https://openalex.org/W1994506742","https://openalex.org/W1994968612","https://openalex.org/W1995087187","https://openalex.org/W1996342509","https://openalex.org/W1998108947","https://openalex.org/W2000254868","https://openalex.org/W2009471270","https://openalex.org/W2009509502","https://openalex.org/W2013972132","https://openalex.org/W2015915616","https://openalex.org/W2017835340","https://openalex.org/W2018150265","https://openalex.org/W2018600328","https://openalex.org/W2019619229","https://openalex.org/W2019744607","https://openalex.org/W2020542402","https://openalex.org/W2023928413","https://openalex.org/W2025562232","https://openalex.org/W2027461974","https://openalex.org/W2028440011","https://openalex.org/W2028908252","https://openalex.org/W2029369726","https://openalex.org/W2029441567","https://openalex.org/W2031019580","https://openalex.org/W2031298372","https://openalex.org/W2031802191","https://openalex.org/W2035272067","https://openalex.org/W2036080658","https://openalex.org/W2036561818","https://openalex.org/W2036922942","https://openalex.org/W2037546764","https://openalex.org/W2038372594","https://openalex.org/W2039412129","https://openalex.org/W2041047203","https://openalex.org/W2041849614","https://openalex.org/W2042636542","https://openalex.org/W2046439590","https://openalex.org/W2046726975","https://openalex.org/W2048822538","https://openalex.org/W2048885992","https://openalex.org/W2051067004","https://openalex.org/W2052157498","https://openalex.org/W2052767109","https://openalex.org/W2054853420","https://openalex.org/W2055057185","https://openalex.org/W2057673975","https://openalex.org/W2057702371","https://openalex.org/W2060144215","https://openalex.org/W2060391020","https://openalex.org/W2060746246","https://openalex.org/W2061078070","https://openalex.org/W2061581252","https://openalex.org/W2062462877","https://openalex.org/W2063432830","https://openalex.org/W2064826444","https://openalex.org/W2065770997","https://openalex.org/W2065915163","https://openalex.org/W2067853451","https://openalex.org/W2069008664","https://openalex.org/W2069285547","https://openalex.org/W2069654377","https://openalex.org/W2070858181","https://openalex.org/W2071300717","https://openalex.org/W2077525285","https://openalex.org/W2078085246","https://openalex.org/W2078560823","https://openalex.org/W2079267703","https://openalex.org/W2083010593","https://openalex.org/W2083107730","https://openalex.org/W2083190822","https://openalex.org/W2089693526","https://openalex.org/W2089941368","https://openalex.org/W2089972364","https://openalex.org/W2092199526","https://openalex.org/W2092856811","https://openalex.org/W2102581601","https://openalex.org/W2103825820","https://openalex.org/W2105842004","https://openalex.org/W2105888055","https://openalex.org/W2106804637","https://openalex.org/W2109131377","https://openalex.org/W2110265381","https://openalex.org/W2113000828","https://openalex.org/W2114611316","https://openalex.org/W2125148255","https://openalex.org/W2125551012","https://openalex.org/W2126770666","https://openalex.org/W2127734104","https://openalex.org/W2128315172","https://openalex.org/W2128635872","https://openalex.org/W2128879247","https://openalex.org/W2129761526","https://openalex.org/W2129995310","https://openalex.org/W2132042949","https://openalex.org/W2132629607","https://openalex.org/W2135328894","https://openalex.org/W2136514461","https://openalex.org/W2140465906","https://openalex.org/W2141474275","https://openalex.org/W2142314320","https://openalex.org/W2148713472","https://openalex.org/W2149830384","https://openalex.org/W2150158773","https://openalex.org/W2150527746","https://openalex.org/W2150881786","https://openalex.org/W2153903162","https://openalex.org/W2158763887","https://openalex.org/W2158821132","https://openalex.org/W2159266419","https://openalex.org/W2162331245","https://openalex.org/W2162700367","https://openalex.org/W2164679484","https://openalex.org/W2164744505","https://openalex.org/W2166220683","https://openalex.org/W2175202929","https://openalex.org/W2178610289","https://openalex.org/W2328394317","https://openalex.org/W2409075142","https://openalex.org/W2414189439","https://openalex.org/W2414693889","https://openalex.org/W2419624695","https://openalex.org/W2950606382"],"related_works":["https://openalex.org/W2765176356","https://openalex.org/W2003006345","https://openalex.org/W2027609269","https://openalex.org/W1832040010","https://openalex.org/W1537277961","https://openalex.org/W1557513215","https://openalex.org/W2222347016","https://openalex.org/W4230753930","https://openalex.org/W2012004265","https://openalex.org/W2123169446"],"abstract_inverted_index":{"Fatty":[0],"acids,":[1],"polyketides":[2],"and":[3,20,47,115,197,255,293,372,384],"nonribosomal":[4],"peptides":[5],"are":[6],"three":[7],"classes":[8,55],"of":[9,29,41,56,62,102,129,142,152,159,184,211,224,234,241,266,273,291,306,308,327,339,355,369,380],"natural":[10],"products":[11],"that":[12,23,105,187],"play":[13],"critical":[14],"roles":[15],"in":[16,344],"human":[17],"health,":[18],"disease":[19],"therapy.":[21],"Investigations":[22],"lead":[24,390],"to":[25,37,88,94,122,126,155,162,170,176,204,208,237,244,252,318,357,387,395],"a":[26,67,72,86,168,274,320,389,397],"broader":[27],"understanding":[28],"their":[30,63],"biosynthesis":[31],"may":[32],"one":[33],"day":[34],"allow":[35],"scientists":[36],"engineer":[38],"the":[39,59,100,110,127,140,150,157,182,192,209,222,232,239,259,263,271,289,303,325,340,353,367],"biomanufacture":[40],"novel":[42],"compounds":[43,57],"with":[44,66,113,195],"tailored":[45],"chemistries":[46],"biological":[48],"activities.":[49],"A":[50,74],"unifying":[51],"characteristic":[52],"between":[53],"these":[54,163,245,309],"is":[58],"posttranslational":[60],"modification":[61],"synthase":[64,97,179],"enzymes":[65,98,180],"4'-phosphopantetheinyl":[68],"(4'-PP)":[69],"arm":[70],"from":[71,131,213],"coenzyme":[73],"(CoA)":[75],"donor,":[76],"installed":[77],"by":[78,139,221,324],"phosphopantetheinyl":[79],"transferase":[80],"(PPTase).":[81],"We":[82,118,200],"have":[83,106,119,165,188,201],"previously":[84,166],"described":[85,167,343],"method":[87,169],"chemoenzymatically":[89,171],"install":[90,172],"modified":[91,173],"4'-PP":[92,174],"arms":[93,175],"heterologously":[95,177],"expressed":[96,178],"through":[99,181,374],"use":[101,151,183,233],"CoA":[103,185],"analogues":[104,186],"been":[107,189],"alkylated":[108,190],"at":[109,191],"thiol":[111,193],"terminus":[112,194],"fluorescent":[114,196],"affinity":[116,198],"probes.":[117,199],"since":[120,202],"sought":[121,203],"extend":[123,205],"this":[124,135,206,217,253,300,313,333,348,359],"technique":[125,161,207,243],"isolation":[128,210],"synthases":[130,212],"producer":[132,214],"organisms,":[133,215],"but":[134,216],"application":[136,218],"was":[137,219],"complicated":[138,220],"efficiency":[141,223],"endogenous":[143,225,286],"modification.":[144,226],"This":[145,227],"dissertation":[146,228,314],"focuses":[147,229],"on":[148,230],"evaluating":[149,231],"PPTase":[153,235,296,398],"inhibitors":[154,236],"enable":[156,238],"extension":[158,240],"our":[160,242],"organisms.We":[164],"organisms.":[246],"Chapter":[247,256,268,282,335,345,350,364],"1":[248],"provides":[249],"an":[250,375],"introduction":[251],"work,":[254],"2":[257],"reviews":[258],"current":[260],"literature":[261],"regarding":[262],"site-specific":[264],"labeling":[265],"proteins.":[267],"3":[269,346],"details":[270,352,385],"development":[272],"homogenous":[275],"resonance":[276],"energy":[277],"transfer":[278],"assay":[279,341],"for":[280,284,299,347,392],"PPTase.":[281],"4":[283],"confirms":[285],"phosphopantetheinylation":[287],"as":[288],"source":[290],"complication,":[292],"evaluates":[294],"known":[295],"inhibitor":[297,322],"architectures":[298],"application;":[301],"revealing":[302],"limited":[304],"spectrum":[305],"activity":[307],"compounds.":[310],"As":[311],"such,":[312],"also":[315],"reports":[316],"efforts":[317,386],"identify":[319],"new":[321],"scaffolds":[323],"screening":[326],"large":[328,360],"chemical":[329,399],"libraries.":[330],"Studies":[331],"toward":[332],"include":[334],"5,":[336],"describing":[337],"optimization":[338,394],"process":[342],"purpose.":[349],"6":[351,373],"preparation":[354],"reporters":[356],"support":[358],"scale":[361],"robotic":[362],"screen.":[363],"7":[365],"applies":[366],"results":[368],"Chapters":[370],"5":[371],"automated":[376],"high":[377],"throughput":[378],"screen":[379],"over":[381],"311,000":[382],"compounds,":[383],"nominate":[388],"structure":[391],"further":[393],"develop":[396],"probe":[400]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
