{"id":"https://openalex.org/W26591985","doi":null,"title":"[Porcine kidney extract contains a specific inhibitor of the ouabain-sensitive alpha2-isoform of Na, K-ATPase present in rat diaphragm fibres].","display_name":"[Porcine kidney extract contains a specific inhibitor of the ouabain-sensitive alpha2-isoform of Na, K-ATPase present in rat diaphragm fibres].","publication_year":2003,"publication_date":"2003-11-01","ids":{"openalex":"https://openalex.org/W26591985","mag":"26591985","pmid":"https://pubmed.ncbi.nlm.nih.gov/14758659"},"language":"ru","primary_location":{"id":"pmid:14758659","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14758659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077811157","display_name":"Igor I. Krivoi","orcid":"https://orcid.org/0000-0002-2690-9226","observed_orcids":["https://orcid.org/0000-0002-2690-9226"]},"institutions":[{"id":"https://openalex.org/I172901346","display_name":"St Petersburg University","ror":"https://ror.org/023znxa73","country_code":"RU","type":"education","lineage":["https://openalex.org/I172901346"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"I I Krivo\u012d","raw_affiliation_strings":["St. Petersburg State University, 199034, St. Petersburg, 7/9 Universitetskaya Nab., Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"St. Petersburg State University, 199034, St. Petersburg, 7/9 Universitetskaya Nab., Russia","institution_ids":["https://openalex.org/I172901346"]}]},{"author_position":"middle","author":{"id":null,"display_name":"A N Vasil'ev","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A N Vasil'ev","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018977496","display_name":"Gromova Vv","orcid":"https://orcid.org/0009-0008-1808-6179","observed_orcids":["https://orcid.org/0009-0008-1808-6179"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V V Gromova","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082050156","display_name":"A E Prytkov","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A E Prytkov","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088109117","display_name":"Irina I. Marakhova","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I I Marakhova","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5030906674","display_name":"\u041a\u0440\u0430\u0432\u0446\u043e\u0432\u0430 \u0412\u0438\u043e\u043b\u0435\u0442\u0442\u0430 \u0412\u0430\u0441\u0438\u043b\u044c\u0435\u0432\u043d\u0430","orcid":"https://orcid.org/0000-0002-3382-5848","observed_orcids":["https://orcid.org/0000-0002-3382-5848"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"V V Kravtsova","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083200304","display_name":"Maxim G. Dobretsov","orcid":"https://orcid.org/0000-0001-6007-9775","observed_orcids":["https://orcid.org/0000-0001-6007-9775"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M G Dobretsov","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5064386338","display_name":"Frederic Mandel","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"F Mandel","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00196543,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"89","issue":"11","first_page":"1340","last_page":"51"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10724","display_name":"Ion Transport and Channel Regulation","score":0.9843999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10493","display_name":"Ion channel regulation and function","score":0.009100000374019146,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12763","display_name":"ATP Synthase and ATPases Research","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/na-k-atpase-alpha2-isoform","display_name":"Na,K-ATPase alpha2 isoform","score":0.9330000281333923},{"id":"https://openalex.org/keywords/ouabain","display_name":"ouabain","score":0.7319999933242798},{"id":"https://openalex.org/keywords/diaphragm","display_name":"diaphragm","score":0.6779999732971191},{"id":"https://openalex.org/keywords/acetylcholine","display_name":"acetylcholine","score":0.8799999952316284},{"id":"https://openalex.org/keywords/endogenous-digitalis-like-factor","display_name":"endogenous digitalis-like factor","score":0.9169999957084656},{"id":"https://openalex.org/keywords/cholinergic-modulation","display_name":"cholinergic modulation","score":0.6579999923706055}],"concepts":[{"id":"https://openalex.org/C2779133857","wikidata":"https://www.wikidata.org/wiki/Q285911","display_name":"Ouabain","level":3,"score":0.9438875913619995},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.7381840944290161},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.517096757888794},{"id":"https://openalex.org/C24998067","wikidata":"https://www.wikidata.org/wiki/Q4114622","display_name":"Stimulation","level":2,"score":0.49637681245803833},{"id":"https://openalex.org/C64943373","wikidata":"https://www.wikidata.org/wiki/Q2651003","display_name":"Alpha (finance)","level":4,"score":0.4822881519794464},{"id":"https://openalex.org/C23265538","wikidata":"https://www.wikidata.org/wiki/Q300033","display_name":"ATPase","level":3,"score":0.4492895305156708},{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.43632858991622925},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3634796440601349},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3570539951324463},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.35005831718444824},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.305156946182251},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.2360665500164032},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.09603071212768555},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.07710656523704529},{"id":"https://openalex.org/C49453240","wikidata":"https://www.wikidata.org/wiki/Q1592163","display_name":"Construct validity","level":3,"score":0.0},{"id":"https://openalex.org/C2775944032","wikidata":"https://www.wikidata.org/wiki/Q22907659","display_name":"Patient satisfaction","level":2,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C159110408","wikidata":"https://www.wikidata.org/wiki/Q121176","display_name":"Nursing","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000254","descriptor_name":"Sodium-Potassium-Exchanging ATPase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003964","descriptor_name":"Diaphragm","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D003964","descriptor_name":"Diaphragm","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D003964","descriptor_name":"Diaphragm","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D007668","descriptor_name":"Kidney","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008564","descriptor_name":"Membrane Potentials","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D010042","descriptor_name":"Ouabain","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014020","descriptor_name":"Tissue Extracts","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D014020","descriptor_name":"Tissue Extracts","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D051381","descriptor_name":"Rats","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:14758659","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14758659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova","raw_type":"Journal Article"},{"id":"mag:26591985","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14758659","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.5683000087738037,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1991579477","https://openalex.org/W2437817005","https://openalex.org/W2151153358","https://openalex.org/W2396068835","https://openalex.org/W3017311476","https://openalex.org/W2046530233","https://openalex.org/W2143791670","https://openalex.org/W2149584537","https://openalex.org/W2162040432","https://openalex.org/W2061921862"],"abstract_inverted_index":{"In":[0],"experiments":[1],"on":[2],"isolated":[3],"rat":[4,115,166],"diaphragm":[5],"muscle,":[6],"acetylcholine":[7],"(100":[8],"nmol/l)":[9,38],"hyperpolarized":[10],"muscle":[11,117],"fibres":[12],"due":[13],"to":[14,58,78],"activation":[15],"of":[16,21,45,65,72,86,107,122,195],"the":[17,54,66,91,102,118,131,165,191],"alpha":[18,103,119,132,167,192],"2":[19,133,168,193],"isoform":[20,106,121,134,169,194],"Na,K-ATPase.":[22,196],"This":[23],"hyperpolarization":[24],"was":[25,80],"blocked":[26],"in":[27,76,96,114],"a":[28,43,59,70,136,155,181],"dose-dependent":[29],"manner":[30],"by":[31,42,143],"ouabain":[32],"(K0.5":[33],"=":[34,104],"8":[35],"+/-":[36],"4":[37],"as":[39,41,180],"well":[40],"solution":[44],"porcine":[46],"kidney":[47,152],"extract":[48,153],"(10":[49],"kDa":[50],"cut-off":[51],"filtration),":[52],"with":[53],"K0.5":[55],"approximately":[56],"equal":[57],"1:20,000-fold":[60],"dilution.":[61],"The":[62],"inhibitory":[63],"activity":[64],"developed":[67],"slowly":[68],"over":[69],"period":[71],"3":[73],"hours":[74],"and,":[75],"contrast":[77],"ouabain,":[79],"still":[81],"present":[82],"after":[83],"1":[84,105,120],"hour":[85],"washing.":[87],"Ouabain,":[88],"but":[89],"not":[90],"extract,":[92],"inhibits":[93,164],"Rb+":[94],"uptake":[95],"human":[97],"erythrocytes":[98],"that":[99,113,162,188],"only":[100],"express":[101],"Na,":[108],"K-ATPase.":[109],"Our":[110,174],"data":[111],"suggest":[112],"skeletal":[116],"Na,K-ATPase":[123],"is":[124],"primarily":[125],"responsible":[126],"for":[127,186],"ionic":[128],"homeostasis,":[129],"while":[130],"provides":[135],"\"regulatable\"":[137],"function":[138],"and":[139,170],"may":[140],"be":[141,178],"controlled":[142],"cholinergic":[144],"stimulation":[145],"and/or":[146],"endogenous":[147],"digitalis-like":[148],"factors":[149,187],"(EDLFs).":[150],"Porcine":[151],"contains":[154],"factor":[156],"(M.":[157],"W.":[158],"<":[159],"10":[160],"kDa)":[161],"selectively":[163,189],"differs":[171],"from":[172],"ouabain.":[173],"experimental":[175],"protocol":[176],"can":[177],"used":[179],"highly":[182],"sensitive":[183],"physiological":[184],"assay":[185],"inhibit":[190]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}