{"id":"https://openalex.org/W263437072","doi":"https://doi.org/10.1007/978-1-4684-6012-4_41","title":"Comparison of Kinetic Properties of Native and Recombinant Human Cathepsin D","display_name":"Comparison of Kinetic Properties of Native and Recombinant Human Cathepsin D","publication_year":1991,"publication_date":"1991-01-01","ids":{"openalex":"https://openalex.org/W263437072","doi":"https://doi.org/10.1007/978-1-4684-6012-4_41","mag":"263437072","pmid":"https://pubmed.ncbi.nlm.nih.gov/1812725"},"language":"en","primary_location":{"id":"doi:10.1007/978-1-4684-6012-4_41","doi":"https://doi.org/10.1007/978-1-4684-6012-4_41","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4684-6012-4_41","pdf_url":null,"source":{"id":"https://openalex.org/S43790123","display_name":"Advances in experimental medicine and biology","issn_l":"0065-2598","issn":["0065-2598","2214-8019"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in Experimental Medicine and Biology","raw_type":"book-chapter"},"volume":{"id":"https://openalex.org/W2493316313","display_name":"Structure and Function of the Aspartic Proteinases"},"type":"book-chapter","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085065524","display_name":"Paula E. Scarborough","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Paula E. Scarborough","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610","[University of Florida]"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"[University of Florida]","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077871314","display_name":"Gary R. Richo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I145608581","display_name":"University of Miami","ror":"https://ror.org/02dgjyy92","country_code":"US","type":"education","lineage":["https://openalex.org/I145608581"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gary R. Richo","raw_affiliation_strings":["Department of Anatomy and Cell Biology, University of Miami, Miami, Florida, 33101, USA","Department of Anatomy and Cell Biology, University of Miami, Miami, USA","University of Miami,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Cell Biology, University of Miami, Miami, Florida, 33101, USA","institution_ids":["https://openalex.org/I145608581"]},{"raw_affiliation_string":"Department of Anatomy and Cell Biology, University of Miami, Miami, USA","institution_ids":["https://openalex.org/I145608581"]},{"raw_affiliation_string":"University of Miami,","institution_ids":["https://openalex.org/I145608581"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112058728","display_name":"John Kay","orcid":"https://orcid.org/0000-0002-2615-8506","observed_orcids":["https://orcid.org/0000-0002-2615-8506"]},"institutions":[{"id":"https://openalex.org/I97429440","display_name":"University of Wales","ror":"https://ror.org/01se4f844","country_code":"GB","type":"education","lineage":["https://openalex.org/I97429440"]}],"countries":["GB"],"is_corresponding":false,"raw_author_name":"John Kay","raw_affiliation_strings":["Department of Biochemistry, University of Wales College of Cardiff, Cardiff, UK","Department of Biochemistry, University of Wales College of Cardiff, P.O. Box 903, Cardiff, CF1 1ST, Wales, UK","University Of Wales College Of Cardiff"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, Cardiff, UK","institution_ids":["https://openalex.org/I97429440"]},{"raw_affiliation_string":"Department of Biochemistry, University of Wales College of Cardiff, P.O. Box 903, Cardiff, CF1 1ST, Wales, UK","institution_ids":["https://openalex.org/I97429440"]},{"raw_affiliation_string":"University Of Wales College Of Cardiff","institution_ids":["https://openalex.org/I97429440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108822444","display_name":"Gregory E. Conner","orcid":"https://orcid.org/0000-0003-2217-2252","observed_orcids":["https://orcid.org/0000-0003-2217-2252"]},"institutions":[{"id":"https://openalex.org/I145608581","display_name":"University of Miami","ror":"https://ror.org/02dgjyy92","country_code":"US","type":"education","lineage":["https://openalex.org/I145608581"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gregory E. Conner","raw_affiliation_strings":["Department of Anatomy and Cell Biology, University of Miami, Miami, Florida, 33101, USA","Department of Anatomy and Cell Biology, University of Miami, Miami, USA","University of Miami,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Cell Biology, University of Miami, Miami, Florida, 33101, USA","institution_ids":["https://openalex.org/I145608581"]},{"raw_affiliation_string":"Department of Anatomy and Cell Biology, University of Miami, Miami, USA","institution_ids":["https://openalex.org/I145608581"]},{"raw_affiliation_string":"University of Miami,","institution_ids":["https://openalex.org/I145608581"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009470180","display_name":"Ben M. Dunn","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ben M. Dunn","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, USA","[University of Florida]"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, USA","institution_ids":["https://openalex.org/I33213144"]},{"raw_affiliation_string":"[University of Florida]","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.9157,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.74213836,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"306","issue":null,"first_page":"343","last_page":"347"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10620","display_name":"Protease and Inhibitor Mechanisms","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12505","display_name":"Peptidase Inhibition and Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cathepsin-d","display_name":"cathepsin D","score":1.0},{"id":"https://openalex.org/keywords/aspartic-protease","display_name":"aspartic protease","score":0.9290000200271606},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.8830000162124634},{"id":"https://openalex.org/keywords/recombinant-protein","display_name":"recombinant protein","score":0.640999972820282},{"id":"https://openalex.org/keywords/zymogen-activation","display_name":"zymogen activation","score":0.7860000133514404},{"id":"https://openalex.org/keywords/substrate-specificity","display_name":"substrate specificity","score":0.6380000114440918},{"id":"https://openalex.org/keywords/chromogenic-peptide-substrates","display_name":"chromogenic peptide substrates","score":0.9169999957084656},{"id":"https://openalex.org/keywords/inclusion-body-refolding","display_name":"inclusion body refolding","score":0.7900000214576721},{"id":"https://openalex.org/keywords/lysosomal-proteolysis","display_name":"lysosomal proteolysis","score":0.7879999876022339},{"id":"https://openalex.org/keywords/human-placenta","display_name":"human placenta","score":0.7129999995231628},{"id":"https://openalex.org/keywords/human-spleen","display_name":"human spleen","score":0.8560000061988831}],"concepts":[{"id":"https://openalex.org/C2776350231","wikidata":"https://www.wikidata.org/wiki/Q7166509","display_name":"Pepstatin","level":4,"score":0.8302780389785767},{"id":"https://openalex.org/C34695024","wikidata":"https://www.wikidata.org/wiki/Q14902030","display_name":"Cathepsin O","level":4,"score":0.7759311199188232},{"id":"https://openalex.org/C174937957","wikidata":"https://www.wikidata.org/wiki/Q18248582","display_name":"Cathepsin E","level":5,"score":0.736510157585144},{"id":"https://openalex.org/C78553746","wikidata":"https://www.wikidata.org/wiki/Q423561","display_name":"Cathepsin A","level":4,"score":0.7268137335777283},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6477403044700623},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6185349822044373},{"id":"https://openalex.org/C173633252","wikidata":"https://www.wikidata.org/wiki/Q3663475","display_name":"Cathepsin D","level":3,"score":0.6170726418495178},{"id":"https://openalex.org/C81216549","wikidata":"https://www.wikidata.org/wiki/Q18022191","display_name":"Cathepsin L1","level":4,"score":0.5989501476287842},{"id":"https://openalex.org/C51618164","wikidata":"https://www.wikidata.org/wiki/Q5052484","display_name":"Cathepsin H","level":4,"score":0.5780948996543884},{"id":"https://openalex.org/C2778775115","wikidata":"https://www.wikidata.org/wiki/Q245759","display_name":"Zymogen","level":3,"score":0.5315634608268738},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4848517179489136},{"id":"https://openalex.org/C2780034444","wikidata":"https://www.wikidata.org/wiki/Q5052485","display_name":"Cathepsin L","level":4,"score":0.4772883951663971},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4708311855792999},{"id":"https://openalex.org/C167844969","wikidata":"https://www.wikidata.org/wiki/Q426245","display_name":"Cathepsin","level":3,"score":0.4465571641921997},{"id":"https://openalex.org/C175156509","wikidata":"https://www.wikidata.org/wiki/Q1990218","display_name":"Cleavage (geology)","level":3,"score":0.4392138421535492},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36551153659820557},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3645175099372864},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.09250721335411072},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.06616368889808655},{"id":"https://openalex.org/C43369102","wikidata":"https://www.wikidata.org/wiki/Q2307625","display_name":"Fracture (geology)","level":2,"score":0.0},{"id":"https://openalex.org/C151730666","wikidata":"https://www.wikidata.org/wiki/Q7205","display_name":"Paleontology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002402","descriptor_name":"Cathepsin D","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D002402","descriptor_name":"Cathepsin D","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002863","descriptor_name":"Chromogenic Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009842","descriptor_name":"Oligopeptides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016282","descriptor_name":"Aspartic Acid Endopeptidases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D066298","descriptor_name":"In Vitro Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/978-1-4684-6012-4_41","doi":"https://doi.org/10.1007/978-1-4684-6012-4_41","is_oa":false,"landing_page_url":"https://doi.org/10.1007/978-1-4684-6012-4_41","pdf_url":null,"source":{"id":"https://openalex.org/S43790123","display_name":"Advances in experimental medicine and biology","issn_l":"0065-2598","issn":["0065-2598","2214-8019"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","ki-jl-1","medline","norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in Experimental Medicine and Biology","raw_type":"book-chapter"},{"id":"pmid:1812725","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/1812725","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in experimental medicine and biology","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"display_name":"Zero hunger","score":0.6899999976158142,"id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":5,"referenced_works":["https://openalex.org/W622925457","https://openalex.org/W1574841922","https://openalex.org/W1957450506","https://openalex.org/W1986348937","https://openalex.org/W2109502383"],"related_works":["https://openalex.org/W2084347696","https://openalex.org/W2153701967","https://openalex.org/W2024209303","https://openalex.org/W2154959406","https://openalex.org/W2288815267","https://openalex.org/W1550890030","https://openalex.org/W2043452588","https://openalex.org/W2036243377","https://openalex.org/W1964852401","https://openalex.org/W147823892"],"abstract_inverted_index":null,"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}