{"id":"https://openalex.org/W2620120516","doi":"https://doi.org/10.1038/nmeth.4495","title":"High-throughput, image-based screening of pooled genetic-variant libraries","display_name":"High-throughput, image-based screening of pooled genetic-variant libraries","publication_year":2017,"publication_date":"2017-10-30","ids":{"openalex":"https://openalex.org/W2620120516","doi":"https://doi.org/10.1038/nmeth.4495","mag":"2620120516","pmid":"https://pubmed.ncbi.nlm.nih.gov/29083401","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/5958624"},"language":"en","primary_location":{"id":"doi:10.1038/nmeth.4495","doi":"https://doi.org/10.1038/nmeth.4495","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nmeth.4495","pdf_url":null,"source":{"id":"https://openalex.org/S127827428","display_name":"Nature Methods","issn_l":"1548-7091","issn":["1548-7091","1548-7105"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.biorxiv.org/content/biorxiv/early/2017/05/30/143966.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5074666046","display_name":"George Emanuel","orcid":"https://orcid.org/0000-0002-4033-3251","observed_orcids":["https://orcid.org/0000-0002-4033-3251"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"George Emanuel","raw_affiliation_strings":["Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","Graduate Program in Biophysics, Harvard University, Cambridge, Massachusetts, USA","Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA"],"raw_orcid":"https://orcid.org/0000-0002-4033-3251","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Graduate Program in Biophysics, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I136199984"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087838801","display_name":"Jeffrey R. Moffitt","orcid":"https://orcid.org/0000-0002-3836-3101","observed_orcids":["https://orcid.org/0000-0002-3836-3101"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Jeffrey R Moffitt","raw_affiliation_strings":["Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087959577","display_name":"Xiaowei Zhuang","orcid":"https://orcid.org/0000-0002-6034-7853","observed_orcids":["https://orcid.org/0000-0002-6034-7853"]},"institutions":[{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xiaowei Zhuang","raw_affiliation_strings":["Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA"],"raw_orcid":"https://orcid.org/0000-0002-6034-7853","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Chemical Biology and Department of Physics, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I136199984"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA","institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I136199984"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5087838801","https://openalex.org/A5087959577"],"corresponding_institution_ids":["https://openalex.org/I1344073410","https://openalex.org/I136199984"],"apc_list":{"value":12690,"currency":"USD","value_usd":12690},"apc_paid":null,"fwci":3.2112,"has_fulltext":false,"cited_by_count":76,"citation_normalized_percentile":{"value":0.92443999,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"14","issue":"12","first_page":"1159","last_page":"1162"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.6851000189781189,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.6851000189781189,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.1316000074148178,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.03790000081062317,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/image-based-screening","display_name":"image-based screening","score":0.7289999723434448},{"id":"https://openalex.org/keywords/fluorescence-in-situ-hybridization","display_name":"fluorescence in situ hybridization","score":0.8259999752044678},{"id":"https://openalex.org/keywords/single-cell-genotyping","display_name":"single-cell genotyping","score":0.7590000033378601},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.7739999890327454},{"id":"https://openalex.org/keywords/fluorescent-protein-variants","display_name":"fluorescent protein variants","score":0.7730000019073486},{"id":"https://openalex.org/keywords/photostability","display_name":"photostability","score":0.9340000152587891}],"concepts":[{"id":"https://openalex.org/C31467283","wikidata":"https://www.wikidata.org/wiki/Q912147","display_name":"Genotyping","level":4,"score":0.706253170967102},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.604396641254425},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5767319202423096},{"id":"https://openalex.org/C2993967602","wikidata":"https://www.wikidata.org/wiki/Q5532932","display_name":"Genetic variants","level":4,"score":0.5580757856369019},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.5519046187400818},{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.5036322474479675},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.4814550280570984},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.42043134570121765},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.3901960253715515},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.29383036494255066},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.19590801000595093},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017404","descriptor_name":"In Situ Hybridization, Fluorescence","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D058893","descriptor_name":"DNA Barcoding, Taxonomic","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.1038/nmeth.4495","doi":"https://doi.org/10.1038/nmeth.4495","is_oa":false,"landing_page_url":"https://doi.org/10.1038/nmeth.4495","pdf_url":null,"source":{"id":"https://openalex.org/S127827428","display_name":"Nature Methods","issn_l":"1548-7091","issn":["1548-7091","1548-7105"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Methods","raw_type":"journal-article"},{"id":"doi:10.1101/143966","doi":"https://doi.org/10.1101/143966","is_oa":true,"landing_page_url":"https://doi.org/10.1101/143966","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2017/05/30/143966.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:29083401","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/29083401","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature methods","raw_type":"Journal Article"},{"id":"pmh:oai:europepmc.org:4876652","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC5958624","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:5958624","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5958624","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Nat Methods","raw_type":"Text"},{"id":"pmh:oai:dash.harvard.edu:1/37160366","doi":null,"is_oa":true,"landing_page_url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:37160366","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Journal Article"},{"id":"pmh:oai:dash.harvard.edu:1/40050109","doi":null,"is_oa":true,"landing_page_url":"http://nrs.harvard.edu/urn-3:HUL.InstRepos:40050109","pdf_url":null,"source":{"id":"https://openalex.org/S4306401540","display_name":"Digital Access to Scholarship at Harvard (DASH) (Harvard University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I136199984","host_organization_name":"Harvard University","host_organization_lineage":["https://openalex.org/I136199984"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis or Dissertation"}],"best_oa_location":{"id":"doi:10.1101/143966","doi":"https://doi.org/10.1101/143966","is_oa":true,"landing_page_url":"https://doi.org/10.1101/143966","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2017/05/30/143966.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4590526960","display_name":"In situ transcriptome imaging in single cells","funder_award_id":"R01MH113094","funder_id":"https://openalex.org/F4320337346","funder_display_name":"National Institute of Mental Health"}],"funders":[{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2620120516.pdf","grobid_xml":"https://content.openalex.org/works/W2620120516.grobid-xml"},"referenced_works_count":39,"referenced_works":["https://openalex.org/W1965411905","https://openalex.org/W1969241857","https://openalex.org/W1971439989","https://openalex.org/W1973260880","https://openalex.org/W1982873976","https://openalex.org/W1999383152","https://openalex.org/W2008778576","https://openalex.org/W2013412105","https://openalex.org/W2017985531","https://openalex.org/W2023719640","https://openalex.org/W2026592619","https://openalex.org/W2029583307","https://openalex.org/W2033788106","https://openalex.org/W2037780745","https://openalex.org/W2041677964","https://openalex.org/W2042789810","https://openalex.org/W2051943484","https://openalex.org/W2068778527","https://openalex.org/W2070360032","https://openalex.org/W2071218392","https://openalex.org/W2086629747","https://openalex.org/W2107629837","https://openalex.org/W2112562104","https://openalex.org/W2127024395","https://openalex.org/W2129016093","https://openalex.org/W2131395804","https://openalex.org/W2135636849","https://openalex.org/W2144147853","https://openalex.org/W2148641266","https://openalex.org/W2150392611","https://openalex.org/W2152505144","https://openalex.org/W2196795138","https://openalex.org/W2520858613","https://openalex.org/W2561508962","https://openalex.org/W2561754210","https://openalex.org/W2562947047","https://openalex.org/W2577994754","https://openalex.org/W2765891490","https://openalex.org/W4256266823"],"related_works":["https://openalex.org/W2765891490","https://openalex.org/W4250484193","https://openalex.org/W3129998905","https://openalex.org/W4206247338","https://openalex.org/W4220665371","https://openalex.org/W6982342174","https://openalex.org/W2981110120","https://openalex.org/W4412351240","https://openalex.org/W4415938890","https://openalex.org/W4385302648"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":12},{"year":2024,"cited_by_count":6},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":13},{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2017-06-05T00:00:00"}
