{"id":"https://openalex.org/W2617050295","doi":null,"title":"Droplet digital PCR improves minimal residual diseasemonitoring in mature lymphoid tumors","display_name":"Droplet digital PCR improves minimal residual diseasemonitoring in mature lymphoid tumors","publication_year":2014,"publication_date":"2014-01-01","ids":{"openalex":"https://openalex.org/W2617050295","mag":"2617050295"},"language":"en","primary_location":{"id":"mag:2617050295","doi":null,"is_oa":false,"landing_page_url":"https://is.muni.cz/publication/1202744/cs/Droplet-digital-PCR-improves-minimal-residual-disease-monitoring-in-mature-lymphoid-tumors/Drandi-Besse-Ferrero-Passera","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},"volume":null,"type":"article","indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5056141793","display_name":"Daniela Drandi","orcid":"https://orcid.org/0000-0003-1825-1206","observed_orcids":["https://orcid.org/0000-0003-1825-1206"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Daniela Drandi","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-1825-1206","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038003121","display_name":"Lenka Be\u0161\u0161e","orcid":"https://orcid.org/0000-0003-4739-7618","observed_orcids":["https://orcid.org/0000-0003-4739-7618"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lenka Be\u0161\u0161e","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4739-7618","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012645361","display_name":"Simone Ferrero","orcid":"https://orcid.org/0000-0002-9711-1502","observed_orcids":["https://orcid.org/0000-0002-9711-1502"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Simone Ferrero","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9711-1502","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087781254","display_name":"Roberto Passera","orcid":"https://orcid.org/0000-0003-3372-2248","observed_orcids":["https://orcid.org/0000-0003-3372-2248"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roberto Passera","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3372-2248","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079136601","display_name":"Barbara Mantoan","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Barbara Mantoan","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036916107","display_name":"Luigia Monitillo","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Luigia Monitillo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055456835","display_name":"Manuela Gambella","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Manuela Gambella","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007558343","display_name":"Paola Omed\u00e8","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Paola Omed\u00e8","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090512266","display_name":"Roman H\u00e1jek","orcid":"https://orcid.org/0000-0001-6955-6267","observed_orcids":["https://orcid.org/0000-0001-6955-6267"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Roman H\u00e1jek","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-6955-6267","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000403848","display_name":"Umberto Vitolo","orcid":"https://orcid.org/0000-0001-7772-2747","observed_orcids":["https://orcid.org/0000-0001-7772-2747"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Umberto Vitolo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-7772-2747","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053612464","display_name":"Antonio Palumbo","orcid":"https://orcid.org/0000-0003-4588-1772","observed_orcids":["https://orcid.org/0000-0003-4588-1772"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Antonio Palumbo","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-4588-1772","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051021130","display_name":"Sergio Cortelazzo","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Sergio Cortelazzo","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018139001","display_name":"Mario Boccadoro","orcid":"https://orcid.org/0000-0001-8130-5209","observed_orcids":["https://orcid.org/0000-0001-8130-5209"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Mario Boccadoro","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-8130-5209","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006640724","display_name":"Giorgio Inghirami","orcid":"https://orcid.org/0000-0001-5566-0864","observed_orcids":["https://orcid.org/0000-0001-5566-0864","https://orcid.org/0000-0002-5298-6516"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Giorgio Inghirami","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5076353357","display_name":"Marco Ladetto","orcid":"https://orcid.org/0000-0002-8283-2681","observed_orcids":["https://orcid.org/0000-0002-8283-2681"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Marco Ladetto","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8283-2681","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.37006353,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.8393999934196472,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10185","display_name":"Lymphoma Diagnosis and Treatment","score":0.8393999934196472,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10950","display_name":"Acute Lymphoblastic Leukemia research","score":0.044599998742341995,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.031599998474121094,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/droplet-digital-pcr","display_name":"droplet digital PCR","score":0.9639999866485596},{"id":"https://openalex.org/keywords/measurable-residual-disease","display_name":"minimal residual disease","score":0.9509999752044678},{"id":"https://openalex.org/keywords/multiple-myeloma","display_name":"multiple myeloma","score":0.9919999837875366},{"id":"https://openalex.org/keywords/mantle-cell-lymphoma","display_name":"mantle cell lymphoma","score":1.0},{"id":"https://openalex.org/keywords/follicular-lymphoma","display_name":"follicular lymphoma","score":1.0},{"id":"https://openalex.org/keywords/immunoglobulin-heavy-chain-genes","display_name":"immunoglobulin heavy chain genes","score":0.6399999856948853},{"id":"https://openalex.org/keywords/absolute-quantification","display_name":"absolute quantification","score":0.7490000128746033}],"concepts":[{"id":"https://openalex.org/C2779823535","wikidata":"https://www.wikidata.org/wiki/Q6865356","display_name":"Minimal residual disease","level":3,"score":0.7801200151443481},{"id":"https://openalex.org/C48126324","wikidata":"https://www.wikidata.org/wiki/Q5276136","display_name":"Digital polymerase chain reaction","level":4,"score":0.6933721899986267},{"id":"https://openalex.org/C2777058707","wikidata":"https://www.wikidata.org/wiki/Q123251","display_name":"Follicular lymphoma","level":3,"score":0.6649649143218994},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4797307550907135},{"id":"https://openalex.org/C2779338263","wikidata":"https://www.wikidata.org/wiki/Q208414","display_name":"Lymphoma","level":2,"score":0.468472421169281},{"id":"https://openalex.org/C2777525834","wikidata":"https://www.wikidata.org/wiki/Q268713","display_name":"Mantle cell lymphoma","level":3,"score":0.45694029331207275},{"id":"https://openalex.org/C48023723","wikidata":"https://www.wikidata.org/wiki/Q856198","display_name":"Real-time polymerase chain reaction","level":3,"score":0.4429047405719757},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.3882666528224945},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.3625580668449402},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.24448472261428833},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24426302313804626},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.24178633093833923},{"id":"https://openalex.org/C2778461978","wikidata":"https://www.wikidata.org/wiki/Q29496","display_name":"Leukemia","level":2,"score":0.14765509963035583},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.11553791165351868},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"mag:2617050295","doi":null,"is_oa":false,"landing_page_url":"https://is.muni.cz/publication/1202744/cs/Droplet-digital-PCR-improves-minimal-residual-disease-monitoring-in-mature-lymphoid-tumors/Drandi-Besse-Ferrero-Passera","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9476000070571899,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2802372704","https://openalex.org/W1147404897","https://openalex.org/W2920830689","https://openalex.org/W3016696556","https://openalex.org/W135873629","https://openalex.org/W4282941976","https://openalex.org/W4285118245","https://openalex.org/W6887640428","https://openalex.org/W2124088728","https://openalex.org/W2593649206"],"abstract_inverted_index":{"Minimal":[0],"residual":[1],"disease":[2],"(MRD)":[3],"detection":[4],"allowed":[5],"acquisition":[6],"of\nvaluable":[7],"prognostic":[8],"information":[9],"in":[10,134,152],"several":[11],"mature":[12],"lymphoid\ndisorders":[13],"with":[14],"a":[15,68,113],"considerable":[16],"impact":[17],"on":[18,102],"clinical":[19],"research\n(Ferrero":[20],"S.":[21],"2011,":[22],"Pott":[23],"C.":[24,52],"2010).":[25],"Although":[26],"different":[27],"methods":[28],"can\nbe":[29],"used":[30],"for":[31,97,157],"MRD":[32,114,138],"quantification,":[33],"including":[34],"flow":[35],"cytometry":[36],"(FC),\nreal-time":[37],"quantitative":[38],"polymerase":[39],"chain":[40],"reaction":[41],"(qPCR)":[42],"remains\nthe":[43],"most":[44],"validated":[45],"and":[46,155,163],"standardized":[47],"method":[48],"(Ladetto":[49],"M.":[50],"2013,\nPott":[51],"2011).":[53],"However,":[54],"despite":[55],"remarkable":[56],"sensitivity":[57],"and\nspecificity,":[58],"qPCR":[59],"has":[60],"some":[61,123],"drawbacks":[62],"mainly":[63],"related":[64],"to":[65,84,93,106,117],"the":[66,95,108,135,159],"need\nfor":[67],"reference":[69],"standard":[70,99],"curve,":[71],"performed":[72],"by":[73],"target":[74],"serial\ndilutions.":[75],"The":[76],"introduction":[77],"of":[78,110,137],"droplet":[79],"digital":[80],"PCR":[81],"(ddPCR)":[82],"has\nallowed":[83],"overcome":[85,122],"this":[86],"limitation,":[87],"since":[88],"its":[89,127],"absolute\nquantification":[90],"competence":[91],"allows":[92],"avoid":[94],"need":[96],"a\nreference":[98],"curve.":[100],"Based":[101],"these":[103],"considerations,":[104],"we\nsought":[105],"verify":[107,118],"utility":[109],"ddPCR":[111,120],"as":[112],"monitoring":[115],"tool\nand":[116],"whether":[119],"could":[121],"qPCR\nlimitations":[124],"without":[125],"mislay":[126],"critical":[128],"advantages.":[129],"We":[130],"compared\nthe":[131],"two":[132],"approaches,":[133],"context":[136],"evaluation,":[139],"in\nmultiple":[140],"myeloma":[141],"(MM),":[142],"mantle":[143],"cell":[144],"lymphoma":[145,148],"(MCL),":[146],"and\nfollicular":[147],"(FL)":[149,167],"patients":[150],"enrolled":[151],"prospective\nclinical":[153],"trials":[154],"selected":[156],"having":[158],"immunoglobuline\ngene":[160],"(IGH)":[161],"(MM":[162],"MCL)":[164],"or":[165],"Bcl-2/MBR":[166],"molecular":[168],"marker":[169],"at\ndiagnosis.":[170]},"counts_by_year":[],"updated_date":"2026-10-09T09:14:12.587421","created_date":"2017-06-05T00:00:00"}