{"id":"https://openalex.org/W2611401331","doi":"https://doi.org/10.21519/0234-2758-2016-32-6-60-67","title":"Optimization of Medium Composition for Cultivation of CHO Line Cells Producing Adalimumab Monoclonal Antibody to Tumor Necrosis Factor Alfa","display_name":"Optimization of Medium Composition for Cultivation of CHO Line Cells Producing Adalimumab Monoclonal Antibody to Tumor Necrosis Factor Alfa","publication_year":2016,"publication_date":"2016-01-01","ids":{"openalex":"https://openalex.org/W2611401331","doi":"https://doi.org/10.21519/0234-2758-2016-32-6-60-67","mag":"2611401331"},"language":"en","primary_location":{"id":"doi:10.21519/0234-2758-2016-32-6-60-67","doi":"https://doi.org/10.21519/0234-2758-2016-32-6-60-67","is_oa":false,"landing_page_url":"http://doi.org/10.21519/0234-2758-2016-32-6-60-67","pdf_url":null,"source":{"id":"https://openalex.org/S4210167523","display_name":"Biotekhnologiya","issn_l":"0234-2758","issn":["0234-2758","2500-2341"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotekhnologiya","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"E. V. VORONINA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E. V. VORONINA","raw_affiliation_strings":["\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103401979","display_name":"N. V. Lobanova","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"N. V. LOBANOVA","raw_affiliation_strings":["\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5059045497","display_name":"Alexey V. Sukhozhenko","orcid":"https://orcid.org/0000-0003-1152-4929","observed_orcids":["https://orcid.org/0000-0003-1152-4929"]},"institutions":[{"id":"https://openalex.org/I4210163576","display_name":"Genetika","ror":"https://ror.org/05hr7qe62","country_code":"RU","type":"facility","lineage":["https://openalex.org/I4210163576"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"A. V. SUKHOZHENKO","raw_affiliation_strings":["\u0424\u0413\u0423\u041f \u00ab\u0413\u043e\u0441\u0443\u0434\u0430\u0440\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0439 \u043d\u0430\u0443\u0447\u043d\u043e-\u0438\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u0441\u043a\u0438\u0439 \u0438\u043d\u0441\u0442\u0438\u0442\u0443\u0442 \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0438 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438 \u043f\u0440\u043e\u043c\u044b\u0448\u043b\u0435\u043d\u043d\u044b\u0445 \u043c\u0438\u043a\u0440\u043e\u043e\u0440\u0433\u0430\u043d\u0438\u0437\u043c\u043e\u0432\u00bb (\u0413\u043e\u0441\u041d\u0418\u0418\u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0430), \u0433. \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u0424\u0413\u0423\u041f \u00ab\u0413\u043e\u0441\u0443\u0434\u0430\u0440\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0439 \u043d\u0430\u0443\u0447\u043d\u043e-\u0438\u0441\u0441\u043b\u0435\u0434\u043e\u0432\u0430\u0442\u0435\u043b\u044c\u0441\u043a\u0438\u0439 \u0438\u043d\u0441\u0442\u0438\u0442\u0443\u0442 \u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0438 \u0438 \u0441\u0435\u043b\u0435\u043a\u0446\u0438\u0438 \u043f\u0440\u043e\u043c\u044b\u0448\u043b\u0435\u043d\u043d\u044b\u0445 \u043c\u0438\u043a\u0440\u043e\u043e\u0440\u0433\u0430\u043d\u0438\u0437\u043c\u043e\u0432\u00bb (\u0413\u043e\u0441\u041d\u0418\u0418\u0433\u0435\u043d\u0435\u0442\u0438\u043a\u0430), \u0433. \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":["https://openalex.org/I4210163576"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053314789","display_name":"A. A. Klishin","orcid":"https://orcid.org/0000-0002-5497-6688","observed_orcids":["https://orcid.org/0000-0002-5497-6688"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"A. A. KLISHIN","raw_affiliation_strings":["\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"I. N. SAVINOVA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"I. N. SAVINOVA","raw_affiliation_strings":["\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013660444","display_name":"Yu. A. Seryogin","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yu. A. SERYOGIN","raw_affiliation_strings":["\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"\u041e\u041e\u041e \u00ab\u0424\u0430\u0440\u043c\u0430\u043f\u0430\u0440\u043a\u00bb, \u041c\u043e\u0441\u043a\u0432\u0430","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.15355522,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"32","issue":"6","first_page":"60","last_page":"67"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adalimumab","display_name":"adalimumab","score":0.9879999756813049},{"id":"https://openalex.org/keywords/cho-cell-culture","display_name":"CHO cell culture","score":0.6579999923706055},{"id":"https://openalex.org/keywords/culture-medium-optimization","display_name":"culture medium optimization","score":0.7400000095367432},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.7979999780654907},{"id":"https://openalex.org/keywords/oligosaccharides","display_name":"oligosaccharides","score":0.7829999923706055},{"id":"https://openalex.org/keywords/fucosylated-glycans","display_name":"fucosylated glycans","score":0.8629999756813049},{"id":"https://openalex.org/keywords/antibody-productivity","display_name":"antibody productivity","score":0.5400000214576721}],"concepts":[{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.775250256061554},{"id":"https://openalex.org/C2780132546","wikidata":"https://www.wikidata.org/wiki/Q348260","display_name":"Adalimumab","level":3,"score":0.5643414258956909},{"id":"https://openalex.org/C17991360","wikidata":"https://www.wikidata.org/wiki/Q21173843","display_name":"Tumor necrosis factor alpha","level":2,"score":0.5534541606903076},{"id":"https://openalex.org/C40231798","wikidata":"https://www.wikidata.org/wiki/Q1333743","display_name":"Composition (language)","level":2,"score":0.48765766620635986},{"id":"https://openalex.org/C198352243","wikidata":"https://www.wikidata.org/wiki/Q37105","display_name":"Line (geometry)","level":2,"score":0.46309804916381836},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.455655574798584},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.41349104046821594},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37140533328056335},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.3683595657348633},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32204920053482056},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3000885844230652},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.24077388644218445},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2067316770553589},{"id":"https://openalex.org/C142362112","wikidata":"https://www.wikidata.org/wiki/Q735","display_name":"Art","level":0,"score":0.11423566937446594},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10415142774581909},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.08738100528717041},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C124952713","wikidata":"https://www.wikidata.org/wiki/Q8242","display_name":"Literature","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.21519/0234-2758-2016-32-6-60-67","doi":"https://doi.org/10.21519/0234-2758-2016-32-6-60-67","is_oa":false,"landing_page_url":"http://doi.org/10.21519/0234-2758-2016-32-6-60-67","pdf_url":null,"source":{"id":"https://openalex.org/S4210167523","display_name":"Biotekhnologiya","issn_l":"0234-2758","issn":["0234-2758","2500-2341"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":["poland-20","russia-white-list-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotekhnologiya","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.46810001134872437,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2131231783","https://openalex.org/W7037039966","https://openalex.org/W7169776708","https://openalex.org/W7197038754","https://openalex.org/W2783298268","https://openalex.org/W2095522936","https://openalex.org/W2924508260","https://openalex.org/W2157531491","https://openalex.org/W4283809767","https://openalex.org/W4411210772"],"abstract_inverted_index":{"A":[0,67],"strategy":[1],"for":[2,134,164,218],"optimization":[3],"of":[4,8,27,36,42,63,70,80,85,98,117,159,183,194,210,252,275,285,303,305,319],"the":[5,37,61,64,90,101,108,118,131,138,141,171,181,184,207,219,226,230,235,250,263,273,300,306,310,320],"glycosylation":[6,185],"profile":[7,186],"Adalimumab,":[9],"a":[10,28,34,40,51,192,276,323],"monoclonal":[11,29],"antibody":[12,30,102,190,208],"to":[13,262],"TNF6":[14],"produced":[15,136],"by":[16,113,137,167],"CH":[17],"O":[18],"culture":[19],"has":[20],"been":[21],"described.":[22],"Adalimumab":[23],"is":[24],"an":[25,316],"analog":[26],"that":[31,127,205,225],"serves":[32],"as":[33,257],"substance":[35],"Humira":[38],"preparation,":[39],"product":[41],"Abbot":[43],"GmbH":[44],"&":[45],"Co.":[46],"KG":[47],"(USA).":[48],"Culturing":[49],"in":[50,229,234,246,272,291],"closed":[52],"volume":[53],"with":[54,107,152],"periodical":[55],"feed":[56,204],"addition":[57],"(fed-batch":[58],"regime)":[59],"was":[60,125,161,216,223,269],"basis":[62],"developed":[65],"technology.":[66],"great":[68],"number":[69],"media":[71],"and":[72,88,104,123,145,149,187,199,238,255],"feeds":[73],"from":[74,100],"various":[75],"manufacturers":[76],"were":[77,111,121,244,313],"tested.":[78],"All":[79],"them":[81],"contained":[82,130,174],"no":[83],"components":[84,259],"animal":[86],"origin":[87],"supported":[89],"CHO":[91,139,168],"cell":[92],"line":[93],"suspension":[94],"cultivation.":[95],"The":[96,157,266,283],"analysis":[97,182],"separated":[99],"oligosaccharides":[103],"their":[105,258],"comparison":[106],"original":[109,236,307],"preparation":[110,120],"performed":[112],"chromatomass-spectrometry.":[114],"Three":[115],"lots":[116],"obtained":[119,166,231],"analyzed,":[122],"it":[124],"shown":[126],"they":[128,297,312],"all":[129],"typical":[132,163],"glycans":[133,151,160,254],"antibodies":[135,165],"cells:":[140],"complex":[142],"fucosylated":[143],"G0F/G1F/G2F":[144],"nonfucosylated":[146],"G0/G1":[147],"forms,":[148],"also":[150,162],"5":[153],"mannose":[154],"residues":[155],"(Man5).":[156],"ratio":[158],"cells":[169],"culturing:":[170],"predominating":[172],"forms":[173],"fucose":[175,239],"rather":[176],"than":[177],"galactose.":[178],"Based":[179],"on":[180,189,294,309],"data":[188],"productivity,":[191],"combination":[193],"Dynamis":[195],"(Gibco,":[196],"USA)":[197],"medium":[198],"ActiCHOA/B":[200],"(GE":[201],"Healthcare,":[202],"Austria)":[203],"provided":[206],"productivity":[209],"1.58":[211],"g/L":[212],"during":[213,322],"fed-batch":[214],"culturing":[215],"selected":[217],"further":[220],"investigation.":[221],"It":[222],"established":[224],"galactose":[227],"(19.6%":[228],"versus":[232,241,281],"23.5%":[233],"preparation)":[237],"(85.7%":[240],"88.7%)":[242],"content":[243,274],"similar":[245],"both":[247],"preparations,":[248],"i.e.":[249],"amounts":[251],"main":[253],"monosacharides":[256],"are":[260,298],"close":[261],"desired":[264],"status.":[265],"significant":[267],"difference":[268],"only":[270],"observed":[271,314],"minor":[277],"glycan,":[278],"Man5":[279],"(1.7%":[280],"6.3%).":[282],"importance":[284],"those":[286],"differences":[287],"can":[288],"be":[289],"assessed":[290],"two":[292],"ways:":[293],"one":[295],"hand,":[296],"beyond":[299],"permissible":[301],"limits":[302],"variability":[304],"preparation;":[308],"other,":[311],"at":[315],"early":[317],"stage":[318],"technology,":[321],"small-volu-me":[324],"culturing.":[325]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2017-05-12T00:00:00"}