{"id":"https://openalex.org/W2608196848","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","title":"Alternative CYP3A4 mRNA Isoforms Are Related to Hepatocyte Differentiation, Liver Development, and Response to Drugs","display_name":"Alternative CYP3A4 mRNA Isoforms Are Related to Hepatocyte Differentiation, Liver Development, and Response to Drugs","publication_year":2011,"publication_date":"2011-04-01","ids":{"openalex":"https://openalex.org/W2608196848","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","mag":"2608196848"},"language":"en","primary_location":{"id":"doi:10.1096/fasebj.25.1_supplement.1014.8","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100380713","display_name":"Dan Li","orcid":"https://orcid.org/0000-0002-2958-8851","observed_orcids":["https://orcid.org/0000-0002-2958-8851"]},"institutions":[{"id":"https://openalex.org/I20231570","display_name":"Peking University","ror":"https://ror.org/02v51f717","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570"]},{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Dan Li","raw_affiliation_strings":["Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing, People's Republic of China","Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing, People's Republic of China","institution_ids":["https://openalex.org/I20231570"]},{"raw_affiliation_string":"Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5048345455","display_name":"Roger Gaedigk","orcid":"https://orcid.org/0000-0001-5942-1609","observed_orcids":["https://orcid.org/0000-0001-5942-1609"]},"institutions":[{"id":"https://openalex.org/I1328594524","display_name":"Children's Mercy Hospital","ror":"https://ror.org/04zfmcq84","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1328594524"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Roger Gaedigk","raw_affiliation_strings":["Division of Clinical Pharmacology and Medical Toxicology, Children's Mercy Hospitals and Clinics, Kansas City, MO"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Clinical Pharmacology and Medical Toxicology, Children's Mercy Hospitals and Clinics, Kansas City, MO","institution_ids":["https://openalex.org/I1328594524"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085507659","display_name":"Steven N. Hart","orcid":"https://orcid.org/0000-0001-7714-2734","observed_orcids":["https://orcid.org/0000-0001-7714-2734"]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Steven N. Hart","raw_affiliation_strings":["Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040407750","display_name":"Hans Tregear","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hans Tregear","raw_affiliation_strings":["Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS","institution_ids":["https://openalex.org/I4210128618"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005624600","display_name":"James Steven Leeder","orcid":"https://orcid.org/0000-0001-6688-0504","observed_orcids":["https://orcid.org/0000-0001-6688-0504"]},"institutions":[{"id":"https://openalex.org/I1328594524","display_name":"Children's Mercy Hospital","ror":"https://ror.org/04zfmcq84","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1328594524"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J Steven Leeder","raw_affiliation_strings":["Division of Clinical Pharmacology and Medical Toxicology, Children's Mercy Hospitals and Clinics, Kansas City, MO"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Clinical Pharmacology and Medical Toxicology, Children's Mercy Hospitals and Clinics, Kansas City, MO","institution_ids":["https://openalex.org/I1328594524"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5030329823","display_name":"Xiao\u2010bo Zhong","orcid":"https://orcid.org/0000-0002-6537-5415","observed_orcids":["https://orcid.org/0000-0002-6537-5415"]},"institutions":[{"id":"https://openalex.org/I20231570","display_name":"Peking University","ror":"https://ror.org/02v51f717","country_code":"CN","type":"education","lineage":["https://openalex.org/I20231570"]},{"id":"https://openalex.org/I4210128618","display_name":"University of Kansas Medical Center","ror":"https://ror.org/036c9yv20","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4210128618"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Xiao\u2010bo Zhong","raw_affiliation_strings":["Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing, People's Republic of China","Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology, Peking University School of Basic Medical Sciences, Beijing, People's Republic of China","institution_ids":["https://openalex.org/I20231570"]},{"raw_affiliation_string":"Pharmacology, Toxicology and Therapeutics, Kansas University Medical Center, Kansas City, KS","institution_ids":["https://openalex.org/I4210128618"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3740,"currency":"USD","value_usd":3740},"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"25","issue":"S1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10375","display_name":"Pharmacogenetics and Drug Metabolism","score":0.9750999808311462,"subfield":{"id":"https://openalex.org/subfields/3004","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.01679999940097332,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cyp3a4","display_name":"CYP3A4","score":0.9959999918937683},{"id":"https://openalex.org/keywords/3-untranslated-region","display_name":"3' untranslated region","score":0.6700000166893005},{"id":"https://openalex.org/keywords/intron-retention","display_name":"intron retention","score":0.8309999704360962},{"id":"https://openalex.org/keywords/liver-development","display_name":"liver development","score":0.875},{"id":"https://openalex.org/keywords/hepatocyte-differentiation","display_name":"hepatocyte differentiation","score":1.0},{"id":"https://openalex.org/keywords/rifampicin","display_name":"rifampicin","score":0.8769999742507935},{"id":"https://openalex.org/keywords/phenobarbital","display_name":"phenobarbital","score":1.0},{"id":"https://openalex.org/keywords/dexamethasone","display_name":"dexamethasone","score":0.9990000128746033},{"id":"https://openalex.org/keywords/heparg-cells","display_name":"HepaRG cells","score":0.8980000019073486},{"id":"https://openalex.org/keywords/cytochrome-p450","display_name":"cytochrome P450","score":0.8999999761581421}],"concepts":[{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.8261699676513672},{"id":"https://openalex.org/C109650736","wikidata":"https://www.wikidata.org/wiki/Q424440","display_name":"CYP3A4","level":4,"score":0.7682592868804932},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6001086831092834},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5764972567558289},{"id":"https://openalex.org/C89604277","wikidata":"https://www.wikidata.org/wiki/Q424345","display_name":"Untranslated region","level":4,"score":0.5187273621559143},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.46149834990501404},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4607784152030945},{"id":"https://openalex.org/C187882448","wikidata":"https://www.wikidata.org/wiki/Q283478","display_name":"Complementary DNA","level":3,"score":0.44837507605552673},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.36903858184814453},{"id":"https://openalex.org/C526171541","wikidata":"https://www.wikidata.org/wiki/Q407693","display_name":"Cytochrome P450","level":3,"score":0.34585249423980713},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2485349178314209},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.15401318669319153},{"id":"https://openalex.org/C62231903","wikidata":"https://www.wikidata.org/wiki/Q1057","display_name":"Metabolism","level":2,"score":0.10400733351707458}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1096/fasebj.25.1_supplement.1014.8","doi":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","is_oa":false,"landing_page_url":"https://doi.org/10.1096/fasebj.25.1_supplement.1014.8","pdf_url":null,"source":{"id":"https://openalex.org/S25293849","display_name":"The FASEB Journal","issn_l":"0892-6638","issn":["0892-6638","1530-6860"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The FASEB Journal","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1605168009","display_name":null,"funder_award_id":"1R01GM087376\u201001A1","funder_id":"https://openalex.org/F4320310131","funder_display_name":"University of Kansas"},{"id":"https://openalex.org/G1828584993","display_name":null,"funder_award_id":"5P20\u2010RR021940","funder_id":"https://openalex.org/F4320310131","funder_display_name":"University of Kansas"},{"id":"https://openalex.org/G3706881391","display_name":null,"funder_award_id":"1R01HD058556\u201001A1","funder_id":"https://openalex.org/F4320310131","funder_display_name":"University of Kansas"},{"id":"https://openalex.org/G7679081802","display_name":null,"funder_award_id":"1R01ES019487\u201001A1","funder_id":"https://openalex.org/F4320310131","funder_display_name":"University of Kansas"}],"funders":[{"id":"https://openalex.org/F4320310131","display_name":"University of Kansas","ror":"https://ror.org/001tmjg57"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2098004164","https://openalex.org/W7099020601","https://openalex.org/W2087922132","https://openalex.org/W2136904160","https://openalex.org/W2076653387","https://openalex.org/W4403597595","https://openalex.org/W4233853770","https://openalex.org/W2068169313","https://openalex.org/W1990473863","https://openalex.org/W2041096981"],"abstract_inverted_index":{"Cytochrome":[0],"P450":[1],"3A4":[2],"(CYP3A4)":[3],"metabolizes":[4],"over":[5,26],"50%":[6],"of":[7,19,28,45,125,134,144,193,203,227],"prescribed":[8],"drugs.":[9,20,70,205],"CYP3A4":[10,51,81,178,194],"activity":[11],"changes":[12],"during":[13,61,159,197],"liver":[14,55,62,93,160,198],"development":[15,199],"and":[16,31,40,56,66,73,92,107,164,170,200,213,219],"the":[17,135,145,153,156,184,191,201,225],"administration":[18,202],"Alternative":[21],"mRNA":[22,52,82,117,179],"isoforms":[23,53,85,118,136],"occur":[24],"in":[25,35,54,67,88,165,190],"90%":[27],"human":[29,89],"genes":[30],"are":[32],"frequently":[33],"observed":[34],"cells":[36,91],"responding":[37],"to":[38,49,69,79,167],"developmental":[39],"environmental":[41],"signals.":[42],"The":[43,115,181],"purpose":[44],"this":[46],"study":[47,174,207],"was":[48,208],"identify":[50,80],"characterize":[57],"their":[58],"differential":[59],"expression":[60,142,196],"development,":[63,161],"hepatocyte":[64,162],"differentiation,":[65,163],"response":[68,166],"cDNA":[71,126],"cloning":[72],"RNA":[74],"sequencing":[75],"(RNA\u2010Seq)":[76],"were":[77,86,119],"used":[78],"isoforms.":[83,180],"Three":[84],"found":[87],"HepaRG":[90],"tissues,":[94],"one":[95,102,108],"represented":[96],"a":[97,104,110],"canonical":[98,157],"full":[99],"length":[100],"mRNA,":[101],"had":[103],"shorter":[105,146,185],"3\u2032\u2010UTR,":[106],"contained":[109],"partial":[111],"intron":[112],"6":[113],"retention.":[114],"alternative":[116],"validated":[120],"by":[121,137,210],"either":[122],"rapid":[123],"amplification":[124],"3\u2032\u2010end":[127],"(3\u2032RACE)":[128],"PCR":[129,139],"or":[130],"end\u2010point":[131],"PCR.":[132],"Quantification":[133],"real\u2010time":[138],"revealed":[140],"that":[141],"levels":[143],"3\u2032\u2010UTR":[147,158,186],"isoform":[148,154,182],"increased":[149],"significantly":[150],"more":[151],"than":[152],"with":[155,183],"rifampicin,":[168],"phenobarbital,":[169],"dexamethasone.":[171],"Conclusion":[172],"This":[173,206],"identified":[175],"two":[176],"novel":[177],"might":[187],"be":[188],"involved":[189],"regulation":[192],"gene":[195],"some":[204],"supported":[209],"1R01GM087376\u201001A1,":[211],"1R01ES019487\u201001A1,":[212],"5P20\u2010RR021940":[214],"(to":[215,229],"X.B.Z),":[216],"1R01HD058556\u201001A1":[217],"(J.S.L),":[218],"Lila":[220],"Self":[221],"Graduate":[222],"Fellowship":[223],"from":[224],"University":[226],"Kansas":[228],"S.N.H.)":[230]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2017-05-05T00:00:00"}