{"id":"https://openalex.org/W2604239362","doi":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","title":"Effects of 5-fluorouracil on endothelial nitric oxide synthase in endothelial cells in different states of confluence","display_name":"Effects of 5-fluorouracil on endothelial nitric oxide synthase in endothelial cells in different states of confluence","publication_year":2004,"publication_date":"2004-07-15","ids":{"openalex":"https://openalex.org/W2604239362","doi":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","mag":"2604239362"},"language":"en","primary_location":{"id":"doi:10.1200/jco.2004.22.14_suppl.3149","doi":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","is_oa":false,"landing_page_url":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","pdf_url":null,"source":{"id":"https://openalex.org/S15137598","display_name":"Journal of Clinical Oncology","issn_l":"0732-183X","issn":["0732-183X","1527-7755"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Oncology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035653088","display_name":"V. M. Casado-Echarren","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"V. M. Casado-Echarren","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065063723","display_name":"C. Abarrategui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"C. Abarrategui","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111599754","display_name":"R. Alvarez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"R. Alvarez","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":null,"display_name":"G. Rubio","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"G. Rubio","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022733274","display_name":"Ana Le\u00f3n","orcid":"https://orcid.org/0009-0005-2767-2274","observed_orcids":["https://orcid.org/0009-0005-2767-2274"]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"A. Le\u00f3n","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103215415","display_name":"Manuel D\u00f3mine","orcid":"https://orcid.org/0000-0003-1634-9832","observed_orcids":["https://orcid.org/0000-0003-1634-9832"]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"M. D\u00f3mine","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103037465","display_name":"Isabel Calvo","orcid":"https://orcid.org/0009-0007-0322-0833","observed_orcids":["https://orcid.org/0009-0007-0322-0833"]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"I. Calvo","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":null,"display_name":"L. G. Est\u00e9vez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"L. G. Est\u00e9vez","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002160272","display_name":"Antonio Jos\u00e9 L\u00f3pez-Farr\u00e9","orcid":"https://orcid.org/0000-0003-0301-067X","observed_orcids":["https://orcid.org/0000-0003-0301-067X"]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"A. L\u00f3pez-Farr\u00e9","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5011244825","display_name":"F. Lobo","orcid":"https://orcid.org/0000-0002-9716-9384","observed_orcids":["https://orcid.org/0000-0002-9716-9384"]},"institutions":[{"id":"https://openalex.org/I4210129357","display_name":"Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz","ror":"https://ror.org/049nvyb15","country_code":"ES","type":"healthcare","lineage":["https://openalex.org/I4210129357"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"F. Lobo","raw_affiliation_strings":["Fundacion Jimenez Diaz, Madrid, Spain"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fundacion Jimenez Diaz, Madrid, Spain","institution_ids":["https://openalex.org/I4210129357"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210129357"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"22","issue":"14_suppl","first_page":"3149","last_page":"3149"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10155","display_name":"Nitric Oxide and Endothelin Effects","score":0.95169997215271,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10422","display_name":"Angiogenesis and VEGF in Cancer","score":0.045499999076128006,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10678","display_name":"Inflammatory mediators and NSAID effects","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/5-fluorouracil","display_name":"5-fluorouracil","score":1.0},{"id":"https://openalex.org/keywords/endothelial-nitric-oxide-synthase","display_name":"endothelial nitric oxide synthase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/endothelial-cells","display_name":"endothelial cells","score":0.9520000219345093},{"id":"https://openalex.org/keywords/endothelial-cell-proliferation","display_name":"endothelial cell proliferation","score":0.847000002861023},{"id":"https://openalex.org/keywords/nitric-oxide","display_name":"nitric oxide","score":0.9660000205039978},{"id":"https://openalex.org/keywords/bovine-aortic-endothelial-cells","display_name":"bovine aortic endothelial cells","score":0.875},{"id":"https://openalex.org/keywords/circulating-tumor-cells","display_name":"circulating tumor cells","score":0.7549999952316284}],"concepts":[{"id":"https://openalex.org/C2778326061","wikidata":"https://www.wikidata.org/wiki/Q1761022","display_name":"Enos","level":4,"score":0.9745951890945435},{"id":"https://openalex.org/C2776992346","wikidata":"https://www.wikidata.org/wiki/Q111140","display_name":"Endothelium","level":2,"score":0.7160371541976929},{"id":"https://openalex.org/C519581460","wikidata":"https://www.wikidata.org/wiki/Q207843","display_name":"Nitric oxide","level":2,"score":0.5728639364242554},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.5646994113922119},{"id":"https://openalex.org/C2992676626","wikidata":"https://www.wikidata.org/wiki/Q408853","display_name":"Endothelial nitric oxide synthase","level":5,"score":0.5466412305831909},{"id":"https://openalex.org/C2777622882","wikidata":"https://www.wikidata.org/wiki/Q417619","display_name":"Nitric oxide synthase","level":3,"score":0.5010299682617188},{"id":"https://openalex.org/C123012128","wikidata":"https://www.wikidata.org/wiki/Q5376430","display_name":"Endothelial stem cell","level":3,"score":0.4948989748954773},{"id":"https://openalex.org/C2908688039","wikidata":"https://www.wikidata.org/wiki/Q408853","display_name":"Nitric Oxide Synthase Type III","level":5,"score":0.4898662269115448},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4624047875404358},{"id":"https://openalex.org/C2776415932","wikidata":"https://www.wikidata.org/wiki/Q48743","display_name":"Western blot","level":3,"score":0.42887449264526367},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.4261060953140259},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3413189649581909},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21223965287208557},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.18272298574447632},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0580366849899292}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1200/jco.2004.22.14_suppl.3149","doi":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","is_oa":false,"landing_page_url":"https://doi.org/10.1200/jco.2004.22.14_suppl.3149","pdf_url":null,"source":{"id":"https://openalex.org/S15137598","display_name":"Journal of Clinical Oncology","issn_l":"0732-183X","issn":["0732-183X","1527-7755"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Oncology","raw_type":"journal-article"},{"id":"doi:10.1200/jco.2004.22.90140.3149","doi":"https://doi.org/10.1200/jco.2004.22.90140.3149","is_oa":false,"landing_page_url":"https://doi.org/10.1200/jco.2004.22.90140.3149","pdf_url":null,"source":{"id":"https://openalex.org/S15137598","display_name":"Journal of Clinical Oncology","issn_l":"0732-183X","issn":["0732-183X","1527-7755"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Oncology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4388757322","https://openalex.org/W4250884419","https://openalex.org/W6981321284","https://openalex.org/W2365502220","https://openalex.org/W2358278407","https://openalex.org/W2119262507","https://openalex.org/W2413854466","https://openalex.org/W1532412386","https://openalex.org/W2368788437","https://openalex.org/W2095334878"],"abstract_inverted_index":{"3149":[0],"Background:":[1],"Some":[2],"studies":[3],"suggest":[4],"a":[5,91,194,206],"direct":[6,92,195],"effect":[7,78,96,196,208],"of":[8,37,44,65,79,97,105,110,118,158,197],"5-fluoruracil":[9],"(5-FU)":[10],"on":[11,81,199,209],"vascular":[12],"endothelium.":[13,26,50,211],"The":[14,116,123],"enzyme":[15,124],"endothelial":[16,55,106,113,171],"nitric-oxide":[17],"synthase":[18],"(eNOS)":[19],"produces":[20],"nitric":[21],"oxide":[22],"(NO)":[23],"in":[24,41,61,136,139,163,186,188],"the":[25,42,49,52,77,82,95,103,200],"It":[27],"is":[28,39,90],"known":[29],"that":[30],"NO":[31],"has":[32],"multiple":[33],"physiological":[34],"functions":[35],"one":[36],"which":[38],"involved":[40],"interaction":[43],"circulating":[45],"tumour":[46],"cells":[47,56,114,172],"with":[48,142,166],"On":[51],"other":[53],"hand,":[54],"(E.C)":[57],"can":[58],"be":[59],"found":[60],"two":[62],"different":[63],"states":[64],"proliferation:":[66],"confluence":[67],"(quiescent)":[68],"and":[69,85,99,102,203],"subconfluence":[70,189],"(proliferating).":[71],"Our":[72],"objetives":[73],"are":[74],"to":[75,86,144,168,177,216],"study":[76],"5-FU":[80,98,119,159,182,198],"eNOS":[83,125,161],"expression":[84,101,138,162,185],"analyse":[87],"if":[88],"there":[89],"relationship":[93],"between":[94],"eNOs":[100,137,184],"state":[104],"growth.":[107],"Methods:":[108],"Culture":[109],"bovine":[111],"aortal":[112],"(B.A.E.C).":[115],"dosage":[117],"was":[120,126],"20":[121],"ugr/mL.":[122],"measured":[127],"using":[128],"Western-blot":[129],"method.":[130],"Results:":[131],"We":[132],"observed":[133],"an":[134],"increase":[135],"subconfluent":[140,164,170],"E.C":[141,146,165,187],"respect":[143,167],"confluent":[145],"(14.6":[147,173],"densitometric":[148],"units":[149],"as":[150,175,204],"opposed":[151,176],"8.97":[152],"DU;p<0.003;":[153],"t":[154],"Student":[155],"method).The":[156],"presence":[157],"reduces":[160,183],"control":[169],"DU":[174],"9.47;":[178],"p<":[179],"0.023).":[180],"Conclusions:":[181],"state.":[190],"This":[191],"finding":[192],"suggests":[193],"proliferating":[201],"endothelium":[202],"well":[205],"possible":[207],"tumoral":[210],"No":[212],"significant":[213],"financial":[214],"relationships":[215],"disclose.":[217]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-04-14T00:00:00"}