{"id":"https://openalex.org/W2602148613","doi":"https://doi.org/10.1117/12.2248537","title":"Using wavelength-normalized optical spectroscopy to improve the accuracy of bacteria growth rate quantification","display_name":"Using wavelength-normalized optical spectroscopy to improve the accuracy of bacteria growth rate quantification","publication_year":2017,"publication_date":"2017-03-28","ids":{"openalex":"https://openalex.org/W2602148613","doi":"https://doi.org/10.1117/12.2248537","mag":"2602148613"},"language":"en","primary_location":{"id":"doi:10.1117/12.2248537","doi":"https://doi.org/10.1117/12.2248537","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2248537","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"},"volume":null,"type":"conference-paper","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036057359","display_name":"Samantha E. McBirney","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samantha E. McBirney","raw_affiliation_strings":["The Univ. of Southern California (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Univ. of Southern California (United States)","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Kristy Trinh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kristy Trinh","raw_affiliation_strings":["The Univ. of Southern California (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Univ. of Southern California (United States)","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013513119","display_name":"Annie Wong\u2010Beringer","orcid":"https://orcid.org/0000-0003-3302-1409","observed_orcids":["https://orcid.org/0000-0003-3302-1409"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Annie Wong-Beringer","raw_affiliation_strings":["The Univ. of Southern California (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Univ. of Southern California (United States)","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5101679309","display_name":"Andrea M. Armani","orcid":"https://orcid.org/0000-0001-9890-5104","observed_orcids":["https://orcid.org/0000-0001-9890-5104"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrea M. Armani","raw_affiliation_strings":["The Univ. of Southern California (United States)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Univ. of Southern California (United States)","institution_ids":["https://openalex.org/I1174212"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1174212"],"apc_list":null,"apc_paid":null,"fwci":0.9369,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.69442533,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"10068","issue":null,"first_page":"1006817","last_page":"1006817"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.7221999764442444,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11324","display_name":"Spectroscopy Techniques in Biomedical and Chemical Research","score":0.7221999764442444,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10977","display_name":"Optical Imaging and Spectroscopy Techniques","score":0.10440000146627426,"subfield":{"id":"https://openalex.org/subfields/2741","display_name":"Radiology, Nuclear Medicine and Imaging"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12167","display_name":"Bacterial Identification and Susceptibility Testing","score":0.030700000002980232,"subfield":{"id":"https://openalex.org/subfields/1308","display_name":"Clinical Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/optical-density-measurement","display_name":"optical density measurement","score":0.828000009059906},{"id":"https://openalex.org/keywords/od600","display_name":"OD600","score":0.8339999914169312},{"id":"https://openalex.org/keywords/pseudomonas-aeruginosa","display_name":"Pseudomonas aeruginosa","score":0.9649999737739563},{"id":"https://openalex.org/keywords/staphylococcus-aureus","display_name":"Staphylococcus aureus","score":1.0},{"id":"https://openalex.org/keywords/cell-concentration-measurement","display_name":"cell concentration measurement","score":0.6959999799728394},{"id":"https://openalex.org/keywords/measurement-error","display_name":"measurement error","score":0.5329999923706055},{"id":"https://openalex.org/keywords/nosocomial-pathogens","display_name":"nosocomial pathogens","score":0.7149999737739563},{"id":"https://openalex.org/keywords/spectrophotometry","display_name":"spectrophotometry","score":0.7160000205039978}],"concepts":[{"id":"https://openalex.org/C6260449","wikidata":"https://www.wikidata.org/wiki/Q41364","display_name":"Wavelength","level":2,"score":0.4917958080768585},{"id":"https://openalex.org/C17741926","wikidata":"https://www.wikidata.org/wiki/Q762045","display_name":"Bacterial growth","level":3,"score":0.44993332028388977},{"id":"https://openalex.org/C2778883795","wikidata":"https://www.wikidata.org/wiki/Q804517","display_name":"Pathogenic bacteria","level":3,"score":0.42522549629211426},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.418049693107605},{"id":"https://openalex.org/C120456961","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Light scattering","level":3,"score":0.4125468134880066},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.3884100317955017},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.38213515281677246},{"id":"https://openalex.org/C186060115","wikidata":"https://www.wikidata.org/wiki/Q30336093","display_name":"Biological system","level":1,"score":0.36394113302230835},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.36200857162475586},{"id":"https://openalex.org/C191486275","wikidata":"https://www.wikidata.org/wiki/Q210028","display_name":"Scattering","level":2,"score":0.33700108528137207},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.3309900760650635},{"id":"https://openalex.org/C49040817","wikidata":"https://www.wikidata.org/wiki/Q193091","display_name":"Optoelectronics","level":1,"score":0.285680890083313},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.18905451893806458},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.1530236303806305},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1117/12.2248537","doi":"https://doi.org/10.1117/12.2248537","is_oa":false,"landing_page_url":"https://doi.org/10.1117/12.2248537","pdf_url":null,"source":{"id":"https://openalex.org/S183492911","display_name":"Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE","issn_l":"0277-786X","issn":["0277-786X","1996-756X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","norway-1"],"host_organization":"https://openalex.org/P4310315543","host_organization_name":"SPIE","host_organization_lineage":["https://openalex.org/P4310315543"],"host_organization_lineage_names":["SPIE"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"SPIE Proceedings","raw_type":"proceedings-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1821086255","https://openalex.org/W1908329802","https://openalex.org/W1975334302","https://openalex.org/W1975738274","https://openalex.org/W1983442486","https://openalex.org/W1984693521","https://openalex.org/W1994424472","https://openalex.org/W2003484929","https://openalex.org/W2006026079","https://openalex.org/W2029615274","https://openalex.org/W2066897136","https://openalex.org/W2076633952","https://openalex.org/W2094506363","https://openalex.org/W2117706768","https://openalex.org/W2127903085","https://openalex.org/W2130868860","https://openalex.org/W2134999596","https://openalex.org/W2137191488","https://openalex.org/W2165928400","https://openalex.org/W2170010570","https://openalex.org/W2299698829","https://openalex.org/W2761545848","https://openalex.org/W2964231477","https://openalex.org/W3102921130","https://openalex.org/W4243501399"],"related_works":["https://openalex.org/W2520242609","https://openalex.org/W7170787938","https://openalex.org/W2809804369","https://openalex.org/W4405721350","https://openalex.org/W4404902391","https://openalex.org/W2952668961","https://openalex.org/W2742166286","https://openalex.org/W2355323006","https://openalex.org/W2614383768","https://openalex.org/W2020153950"],"abstract_inverted_index":{"One":[0],"of":[1,30,78,85,103,175,181,199,216,220,235,238,249],"the":[2,42,55,61,75,86,101,110,134,147,173,217,236,247],"fundamental":[3],"analytical":[4],"measurements":[5,52],"performed":[6],"in":[7,15,26,195],"microbiology":[8],"is":[9,39,139],"monitoring":[10,187],"and":[11,81,106,129,162,179,186,205,209,227],"characterizing":[12],"cell":[13],"concentration":[14],"culture":[16],"media.":[17],"Measurement":[18],"error":[19],"will":[20,229],"give":[21],"rise":[22],"to":[23,34,73,93,126,141,154,207,223,231,242],"reproducibility":[24],"problems":[25],"a":[27,66,176,192,196,213,232,250],"wide":[28,197],"range":[29,198],"applications,":[31],"from":[32,202],"biomanufacturing":[33],"basic":[35],"research.":[36],"Therefore,":[37],"it":[38,170],"critical":[40,193],"that":[41,169],"generated":[43],"results":[44],"are":[45],"consistent.":[46],"Single":[47],"wavelength":[48],"optical":[49,70],"density":[50],"(OD)":[51],"have":[53],"become":[54],"preferred":[56],"approach.":[57],"Here,":[58],"we":[59],"compare":[60],"conventional":[62],"OD600":[63,136],"technique":[64],"with":[65,90,117],"multi-wavelength":[67,97],"normalized":[68],"scattering":[69,130],"spectroscopy":[71],"method":[72,138],"measure":[74],"growth":[76,115,148,157,189,218],"rates":[77,116,158,190],"Pseudomonas":[79],"aeruginosa":[80],"Staphylococcus":[82],"aureus,":[83],"two":[84],"leading":[87,241],"nosocomial":[88],"pathogens":[89],"proven":[91],"abilities":[92],"develop":[94],"resistance.":[95],"The":[96],"normalization":[98],"process":[99],"minimizes":[100],"impact":[102],"bacteria":[104,143,156,188,221],"byproducts":[105],"environmental":[107],"noise":[108],"on":[109],"signal,":[111],"thereby":[112],"accurately":[113],"quantifying":[114],"high":[118],"fidelity":[119],"at":[120,131],"low":[121],"concentrations.":[122],"In":[123],"contrast,":[124],"due":[125],"poor":[127],"absorbance":[128],"600":[132],"nm,":[133],"classic":[135],"measurement":[137],"able":[140],"detect":[142,155],"but":[144],"cannot":[145],"quantify":[146],"rate":[149],"reliably.":[150],"Our":[151],"wavelength-normalization":[152],"protocol":[153],"can":[159],"be":[160],"readily":[161],"easily":[163],"adopted":[164],"by":[165],"research":[166],"labs,":[167],"given":[168],"only":[171],"requires":[172],"use":[174],"standard":[177],"spectrophotometer":[178],"implementation":[180],"straightforward":[182],"data":[183],"analysis.":[184],"Measuring":[185],"play":[191],"role":[194],"settings,":[200],"spanning":[201],"therapeutic":[203],"design":[204],"development":[206,248],"diagnostics":[208],"disease":[210],"prevention.":[211],"Having":[212],"full":[214],"understanding":[215,234],"cycles":[219],"known":[222],"cause":[224],"severe":[225],"infections":[226],"diseases":[228],"lead":[230],"better":[233,243],"pathogenesis":[237],"these":[239],"illnesses,":[240],"treatment":[244],"and,":[245],"ultimately,":[246],"cure.":[251]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2017-04-07T00:00:00"}