{"id":"https://openalex.org/W2597084949","doi":null,"title":"Genomic Analysis of microRNAs in Mouse Skin: Quantification, Biogenesis, Target Recognition and Regulatory Functions","display_name":"Genomic Analysis of microRNAs in Mouse Skin: Quantification, Biogenesis, Target Recognition and Regulatory Functions","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2597084949","mag":"2597084949"},"language":"en","primary_location":{"id":"pmh:oai:cuscholar:8w32r580d","doi":null,"is_oa":true,"landing_page_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","pdf_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","source":{"id":"https://openalex.org/S4306401957","display_name":"CU Scholar (University of Colorado Boulder)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I188538660","host_organization_name":"University of Colorado Boulder","host_organization_lineage":["https://openalex.org/I188538660"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5080414951","display_name":"Zhaojie Zhang","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Zhang, Zhaojie","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5080414951"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/microrna","display_name":"microRNA","score":0.9800000190734863},{"id":"https://openalex.org/keywords/mouse-skin","display_name":"mouse skin","score":0.9649999737739563},{"id":"https://openalex.org/keywords/microrna-biogenesis","display_name":"microRNA biogenesis","score":0.9509999752044678},{"id":"https://openalex.org/keywords/argonaute-proteins","display_name":"Argonaute proteins","score":0.9509999752044678},{"id":"https://openalex.org/keywords/mirna-isoforms","display_name":"miRNA isoforms","score":0.9539999961853027},{"id":"https://openalex.org/keywords/mirna-target-recognition","display_name":"miRNA target recognition","score":0.7649999856948853},{"id":"https://openalex.org/keywords/mir-203","display_name":"miR-203","score":0.906000018119812},{"id":"https://openalex.org/keywords/microrna-expression-profiling","display_name":"miRNA expression profiling","score":0.7940000295639038},{"id":"https://openalex.org/keywords/epidermal-differentiation","display_name":"epidermal differentiation","score":0.8989999890327454},{"id":"https://openalex.org/keywords/stem-cell-self-renewal","display_name":"stem cell self-renewal","score":0.9369999766349792},{"id":"https://openalex.org/keywords/dna-damage-response","display_name":"DNA damage response","score":0.8550000190734863},{"id":"https://openalex.org/keywords/cell-cycle-regulation","display_name":"cell cycle regulation","score":0.8970000147819519}],"concepts":[{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.6781052947044373},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5381107926368713},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.48964935541152954},{"id":"https://openalex.org/C131934819","wikidata":"https://www.wikidata.org/wiki/Q135390","display_name":"Biogenesis","level":3,"score":0.46371057629585266},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.46007686853408813},{"id":"https://openalex.org/C21592294","wikidata":"https://www.wikidata.org/wiki/Q3238407","display_name":"Regulatory sequence","level":4,"score":0.4422880709171295},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3898586332798004},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.3407437205314636}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:cuscholar:8w32r580d","doi":null,"is_oa":true,"landing_page_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","pdf_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","source":{"id":"https://openalex.org/S4306401957","display_name":"CU Scholar (University of Colorado Boulder)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I188538660","host_organization_name":"University of Colorado Boulder","host_organization_lineage":["https://openalex.org/I188538660"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},{"id":"pmh:oai:scholar.colorado.edu:mcdb_gradetds-1024","doi":null,"is_oa":true,"landing_page_url":"https://scholar.colorado.edu/mcdb_gradetds/23","pdf_url":"https://scholar.colorado.edu/mcdb_gradetds/23","source":{"id":"https://openalex.org/S4306401957","display_name":"CU Scholar (University of Colorado Boulder)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I188538660","host_organization_name":"University of Colorado Boulder","host_organization_lineage":["https://openalex.org/I188538660"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Molecular, Cellular, and Developmental Biology Graduate Theses &amp; Dissertations","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:cuscholar:8w32r580d","doi":null,"is_oa":true,"landing_page_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","pdf_url":"https://scholar.colorado.edu/concern/graduate_thesis_or_dissertations/8w32r580d","source":{"id":"https://openalex.org/S4306401957","display_name":"CU Scholar (University of Colorado Boulder)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I188538660","host_organization_name":"University of Colorado Boulder","host_organization_lineage":["https://openalex.org/I188538660"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dissertation"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2597084949.pdf","grobid_xml":"https://content.openalex.org/works/W2597084949.grobid-xml"},"referenced_works_count":157,"referenced_works":["https://openalex.org/W144423133","https://openalex.org/W1481189688","https://openalex.org/W1578977662","https://openalex.org/W1850260711","https://openalex.org/W1958284284","https://openalex.org/W1964872204","https://openalex.org/W1968525732","https://openalex.org/W1970801772","https://openalex.org/W1971099302","https://openalex.org/W1976564658","https://openalex.org/W1979814807","https://openalex.org/W1988249075","https://openalex.org/W1989015932","https://openalex.org/W1991007428","https://openalex.org/W1992941735","https://openalex.org/W1993799748","https://openalex.org/W1998479150","https://openalex.org/W2003952411","https://openalex.org/W2004087690","https://openalex.org/W2004601877","https://openalex.org/W2005356516","https://openalex.org/W2010763857","https://openalex.org/W2011202983","https://openalex.org/W2014946489","https://openalex.org/W2017642141","https://openalex.org/W2018449783","https://openalex.org/W2018507242","https://openalex.org/W2019774078","https://openalex.org/W2019993012","https://openalex.org/W2022243783","https://openalex.org/W2026570544","https://openalex.org/W2027954222","https://openalex.org/W2027986625","https://openalex.org/W2028990252","https://openalex.org/W2031014255","https://openalex.org/W2031837743","https://openalex.org/W2032640887","https://openalex.org/W2033518550","https://openalex.org/W2033533378","https://openalex.org/W2034394760","https://openalex.org/W2034432190","https://openalex.org/W2035340311","https://openalex.org/W2036500104","https://openalex.org/W2039606472","https://openalex.org/W2041693867","https://openalex.org/W2043254922","https://openalex.org/W2043828851","https://openalex.org/W2044285871","https://openalex.org/W2045178946","https://openalex.org/W2045402423","https://openalex.org/W2046314136","https://openalex.org/W2046486243","https://openalex.org/W2046930479","https://openalex.org/W2047271769","https://openalex.org/W2048085228","https://openalex.org/W2049836150","https://openalex.org/W2050066317","https://openalex.org/W2050642406","https://openalex.org/W2052210687","https://openalex.org/W2052677987","https://openalex.org/W2053103314","https://openalex.org/W2054109341","https://openalex.org/W2055043387","https://openalex.org/W2055309264","https://openalex.org/W2059073003","https://openalex.org/W2059168179","https://openalex.org/W2062332984","https://openalex.org/W2062779744","https://openalex.org/W2064557873","https://openalex.org/W2065129911","https://openalex.org/W2070062799","https://openalex.org/W2071596792","https://openalex.org/W2072442043","https://openalex.org/W2072636314","https://openalex.org/W2072943471","https://openalex.org/W2073014932","https://openalex.org/W2082213106","https://openalex.org/W2084807845","https://openalex.org/W2086227770","https://openalex.org/W2087966784","https://openalex.org/W2092018614","https://openalex.org/W2092113020","https://openalex.org/W2095125813","https://openalex.org/W2095388109","https://openalex.org/W2095905881","https://openalex.org/W2096224834","https://openalex.org/W2096744132","https://openalex.org/W2099517697","https://openalex.org/W2099855036","https://openalex.org/W2101117526","https://openalex.org/W2102619694","https://openalex.org/W2107961632","https://openalex.org/W2108234281","https://openalex.org/W2109972881","https://openalex.org/W2110441629","https://openalex.org/W2111831519","https://openalex.org/W2112785261","https://openalex.org/W2113666425","https://openalex.org/W2113934040","https://openalex.org/W2114058102","https://openalex.org/W2114417755","https://openalex.org/W2115559525","https://openalex.org/W2118818674","https://openalex.org/W2121876008","https://openalex.org/W2123023254","https://openalex.org/W2124985265","https://openalex.org/W2125285877","https://openalex.org/W2125855796","https://openalex.org/W2126184583","https://openalex.org/W2128016314","https://openalex.org/W2132691262","https://openalex.org/W2132888671","https://openalex.org/W2135106752","https://openalex.org/W2136991248","https://openalex.org/W2138664009","https://openalex.org/W2139115320","https://openalex.org/W2140692632","https://openalex.org/W2141729181","https://openalex.org/W2144674491","https://openalex.org/W2144696007","https://openalex.org/W2147019494","https://openalex.org/W2148440440","https://openalex.org/W2148612363","https://openalex.org/W2149748804","https://openalex.org/W2150116770","https://openalex.org/W2150518449","https://openalex.org/W2151922790","https://openalex.org/W2152041515","https://openalex.org/W2154841861","https://openalex.org/W2155649150","https://openalex.org/W2156629568","https://openalex.org/W2158010566","https://openalex.org/W2159978426","https://openalex.org/W2160467221","https://openalex.org/W2160697532","https://openalex.org/W2161560970","https://openalex.org/W2161892393","https://openalex.org/W2162674813","https://openalex.org/W2162855805","https://openalex.org/W2163032823","https://openalex.org/W2163106706","https://openalex.org/W2163199119","https://openalex.org/W2164112796","https://openalex.org/W2164286670","https://openalex.org/W2165586566","https://openalex.org/W2166724005","https://openalex.org/W2166903671","https://openalex.org/W2167557255","https://openalex.org/W2169570250","https://openalex.org/W2169590997","https://openalex.org/W2169630576","https://openalex.org/W2171559697","https://openalex.org/W2171997622","https://openalex.org/W2186408667","https://openalex.org/W2327893181","https://openalex.org/W2346111759","https://openalex.org/W7100139990"],"related_works":["https://openalex.org/W1546939152","https://openalex.org/W2196277032","https://openalex.org/W2154793065","https://openalex.org/W1990394449","https://openalex.org/W2156186883","https://openalex.org/W2149146453","https://openalex.org/W2042996013","https://openalex.org/W1908439727","https://openalex.org/W2278086553","https://openalex.org/W1531953029"],"abstract_inverted_index":{"MiRNAs":[0],"are":[1,193,298,369],"a":[2,17,76,96,111,132,143,154,275],"class":[3],"of":[4,20,27,35,42,59,90,118,124,171,175,184,198,213,231,242,245,267,278,288,304,330,340,345],"small":[5,97],"RNAs,":[6],"approximately":[7],"20~22":[8],"nucleotides":[9],"in":[10,92,106,127,251,285,301,313,327],"length.":[11],"These":[12,319],"broadly":[13],"conserved":[14],"molecules":[15],"represent":[16],"novel":[18,265],"layer":[19],"gene":[21],"regulation":[22,303],"that":[23,101,190,223,272,294],"fine-tunes":[24],"the":[25,28,33,39,43,51,81,88,107,115,122,136,182,211,229,236,246,252,286,302,314,322,338,346],"output":[26],"transcriptome":[29],"and":[30,56,121,141,181,202,239,259,307],"contributes":[31],"to":[32,79,134,177,196,316,371],"robustness":[34],"biological":[36],"pathways.":[37],"However,":[38,349],"quantitative":[40,155],"picture":[41],"miRNA":[44,82,160,172,186,221],"pathway":[45,83],"is":[46,208,361],"unclear.":[47],"Fundamental":[48],"questions":[49],"concerning":[50],"biogenesis":[52],"pathway,":[53],"target":[54,237],"recognition":[55,238],"regulatory":[57,240],"functions":[58],"individual":[60,119,178,367],"miRNAs":[61,91,120,176,192,250,368],"within":[62],"mammalian":[63,191],"tissues":[64],"remain":[65],"poorly":[66],"understood.":[67],"In":[68],"this":[69,289],"thesis,":[70],"I":[71,109,130,151,188,217,262,292,336],"employ":[72],"mouse":[73,93,257],"skin":[74,94,357],"as":[75,310,312],"model":[77,258],"system":[78,133],"examine":[80],"with":[84,95,163,210,280,342,363],"genomic":[85],"approaches.\\nI":[86],"profiled":[87],"expression":[89,116,161],"RNA":[98],"cloning":[99],"protocol":[100],"has":[102],"been":[103],"widely":[104],"used":[105],"field.":[108],"observed":[110,354],"significant":[112],"disagreement":[113],"between":[114],"levels":[117,162],"frequencies":[123],"corresponding":[125],"clones":[126],"cDNA":[128,146],"libraries.":[129],"established":[131,153],"benchmark":[135],"biases,":[137],"optimized":[138],"each":[139,214],"step":[140],"achieved":[142],"highly":[144,248,299],"efficient":[145],"library":[147],"construction":[148],"strategy.":[149],"Collectively,":[150],"have":[152,225],"pipeline":[156],"for":[157,228,325,375],"accurately":[158],"profiling":[159],"deep":[164],"sequencing.\\nI":[165],"next":[166],"investigated":[167],"two":[168],"important":[169,226],"aspects":[170],"biogenesis,":[173],"sorting":[174],"Argonaute":[179,200,206,215],"proteins":[180,201,207],"diversity":[183],"mature":[185],"sequences.":[187],"demonstrated":[189],"randomly":[194],"sorted":[195],"one":[197,244],"four":[199],"their":[203],"distribution":[204],"among":[205],"correlated":[209],"abundance":[212],"protein.":[216],"also":[218],"characterized":[219],"many":[220,264,364],"isoforms":[222],"potentially":[224],"implications":[227],"function":[230,241],"miRNAs.\\nI":[232],"then":[233],"focused":[234],"on":[235],"miR-203,":[243],"most":[247],"expressed":[249],"skin.":[253],"With":[254],"an":[255],"inducible":[256],"microarray":[260],"analysis,":[261],"identified":[263],"targets":[266,279,297],"miR-203.":[268],"My":[269],"results":[270,320],"revealed":[271],"miR-203":[273,296,326],"recognizes":[274],"large":[276],"number":[277],"its":[281],"seed":[282],"sequences":[283],"located":[284],"5\u2019":[287],"miRNA.":[290],"Furthermore,":[291],"showed":[293],"these":[295],"enriched":[300],"cell":[305,308],"cycle":[306],"division":[309],"well":[311],"response":[315],"DNA":[317],"damage.":[318],"establish":[321],"widespread":[323],"role":[324],"suppressing":[328],"self-renewal":[329],"stem":[331],"cells":[332],"during":[333,355],"epidermal":[334],"differentiation.\\nFinally,":[335],"studied":[337],"phenotypes":[339,352],"animals":[341],"genetic":[343],"deletion":[344],"miR-143/145":[347],"cluster.":[348],"no":[350],"discernible":[351],"were":[353],"normal":[356],"development.":[358,377],"This":[359],"observation":[360],"consistent":[362],"studies":[365],"where":[366],"shown":[370],"be":[372],"not":[373],"required":[374],"animal":[376]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-10T00:00:00"}
