{"id":"https://openalex.org/W2597052847","doi":"https://doi.org/10.1182/blood-2016-08-735605","title":"Human neutrophils mediate trogocytosis rather than phagocytosis of CLL B cells opsonized with anti-CD20 antibodies","display_name":"Human neutrophils mediate trogocytosis rather than phagocytosis of CLL B cells opsonized with anti-CD20 antibodies","publication_year":2017,"publication_date":"2017-03-14","ids":{"openalex":"https://openalex.org/W2597052847","doi":"https://doi.org/10.1182/blood-2016-08-735605","mag":"2597052847","pmid":"https://pubmed.ncbi.nlm.nih.gov/28288980"},"language":"en","primary_location":{"id":"doi:10.1182/blood-2016-08-735605","doi":"https://doi.org/10.1182/blood-2016-08-735605","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood-2016-08-735605","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010646680","display_name":"Rut Valgardsdottir","orcid":"https://orcid.org/0000-0002-8925-2516","observed_orcids":["https://orcid.org/0000-0002-8925-2516"]},"institutions":[{"id":"https://openalex.org/I4210106645","display_name":"Ospedale Papa Giovanni XXIII","ror":"https://ror.org/01savtv33","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210106645"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Rut Valgardsdottir","raw_affiliation_strings":["Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;"],"raw_orcid":"https://orcid.org/0000-0002-8925-2516","affiliations":[{"raw_affiliation_string":"Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;","institution_ids":["https://openalex.org/I4210106645"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064542411","display_name":"Irene Cattaneo","orcid":"https://orcid.org/0000-0002-5094-7150","observed_orcids":["https://orcid.org/0000-0002-5094-7150"]},"institutions":[{"id":"https://openalex.org/I19630809","display_name":"Mario Negri Institute for Pharmacological Research","ror":"https://ror.org/05aspc753","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I19630809","https://openalex.org/I4210110338","https://openalex.org/I4210153126"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Irene Cattaneo","raw_affiliation_strings":["Department of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri, Istituto di Ricovero e Cura a Carattere Scientifico, Bergamo, Italy; and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri, Istituto di Ricovero e Cura a Carattere Scientifico, Bergamo, Italy; and","institution_ids":["https://openalex.org/I19630809"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067162282","display_name":"Christian Klein","orcid":"https://orcid.org/0000-0001-7594-7280","observed_orcids":["https://orcid.org/0000-0001-7594-7280"]},"institutions":[{"id":"https://openalex.org/I118019719","display_name":"F. Hoffmann-La Roche AG","ror":"https://ror.org/00by1q217","country_code":"CH","type":"company","lineage":["https://openalex.org/I118019719"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Christian Klein","raw_affiliation_strings":["Roche Pharma Research and Early Development, Roche Innovation Center Zurich, Schlieren, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Roche Pharma Research and Early Development, Roche Innovation Center Zurich, Schlieren, Switzerland","institution_ids":["https://openalex.org/I118019719"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035673194","display_name":"Martino Introna","orcid":"https://orcid.org/0000-0001-6216-0892","observed_orcids":["https://orcid.org/0000-0001-6216-0892"]},"institutions":[{"id":"https://openalex.org/I4210106645","display_name":"Ospedale Papa Giovanni XXIII","ror":"https://ror.org/01savtv33","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210106645"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Martino Introna","raw_affiliation_strings":["Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;","institution_ids":["https://openalex.org/I4210106645"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041268006","display_name":"Marina Figliuzzi","orcid":"https://orcid.org/0000-0002-3239-2585","observed_orcids":["https://orcid.org/0000-0002-3239-2585"]},"institutions":[{"id":"https://openalex.org/I19630809","display_name":"Mario Negri Institute for Pharmacological Research","ror":"https://ror.org/05aspc753","country_code":"IT","type":"nonprofit","lineage":["https://openalex.org/I19630809","https://openalex.org/I4210110338","https://openalex.org/I4210153126"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Marina Figliuzzi","raw_affiliation_strings":["Department of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri, Istituto di Ricovero e Cura a Carattere Scientifico, Bergamo, Italy; and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, Istituto di Ricerche Farmacologiche Mario Negri, Istituto di Ricovero e Cura a Carattere Scientifico, Bergamo, Italy; and","institution_ids":["https://openalex.org/I19630809"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5018208681","display_name":"Jos\u00e9e Golay","orcid":"https://orcid.org/0000-0002-7932-909X","observed_orcids":["https://orcid.org/0000-0002-7932-909X"]},"institutions":[{"id":"https://openalex.org/I4210106645","display_name":"Ospedale Papa Giovanni XXIII","ror":"https://ror.org/01savtv33","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210106645"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Jos\u00e9e Golay","raw_affiliation_strings":["Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;"],"raw_orcid":"https://orcid.org/0000-0002-7932-909X","affiliations":[{"raw_affiliation_string":"Center of Cellular Therapy G. Lanzani, Division of Hematology, Azienda Socio Sanitaria Territoriale Papa Giovanni XXIII, Bergamo, Italy;","institution_ids":["https://openalex.org/I4210106645"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5500,"currency":"USD","value_usd":5500},"apc_paid":null,"fwci":10.0516,"has_fulltext":false,"cited_by_count":113,"citation_normalized_percentile":{"value":0.98718679,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":99,"max":100},"biblio":{"volume":"129","issue":"19","first_page":"2636","last_page":"2644"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11157","display_name":"Chronic Lymphocytic Leukemia Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11157","display_name":"Chronic Lymphocytic Leukemia Research","score":0.9804999828338623,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11597","display_name":"Neutrophil, Myeloperoxidase and Oxidative Mechanisms","score":0.002899999963119626,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12672","display_name":"Phagocytosis and Immune Regulation","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trogocytosis","display_name":"trogocytosis","score":0.8769999742507935},{"id":"https://openalex.org/keywords/phagocytosis","display_name":"phagocytosis","score":0.9399999976158142},{"id":"https://openalex.org/keywords/neutrophils","display_name":"neutrophils","score":0.8989999890327454},{"id":"https://openalex.org/keywords/chronic-lymphocytic-leukemia","display_name":"chronic lymphocytic leukemia","score":0.9950000047683716},{"id":"https://openalex.org/keywords/anti-cd20-monoclonal-antibody","display_name":"anti-CD20 monoclonal antibody","score":0.8700000047683716},{"id":"https://openalex.org/keywords/rituximab","display_name":"rituximab","score":0.9340000152587891},{"id":"https://openalex.org/keywords/obinutuzumab","display_name":"obinutuzumab","score":1.0},{"id":"https://openalex.org/keywords/b-cells","display_name":"B cells","score":0.5879999995231628},{"id":"https://openalex.org/keywords/cd20","display_name":"CD20","score":0.6729999780654907},{"id":"https://openalex.org/keywords/antibody-opsonization","display_name":"antibody opsonization","score":0.6019999980926514},{"id":"https://openalex.org/keywords/b-cell-depletion","display_name":"B-cell depletion","score":0.46399998664855957}],"concepts":[{"id":"https://openalex.org/C2777607594","wikidata":"https://www.wikidata.org/wiki/Q389496","display_name":"Obinutuzumab","level":4,"score":0.9371174573898315},{"id":"https://openalex.org/C160448771","wikidata":"https://www.wikidata.org/wiki/Q184726","display_name":"Phagocytosis","level":2,"score":0.7993961572647095},{"id":"https://openalex.org/C89372155","wikidata":"https://www.wikidata.org/wiki/Q420107","display_name":"Opsonin","level":3,"score":0.7716101408004761},{"id":"https://openalex.org/C18031839","wikidata":"https://www.wikidata.org/wiki/Q5009796","display_name":"CD20","level":3,"score":0.566072404384613},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.4986836910247803},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.4434165954589844},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.43012234568595886},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.320108562707901}],"mesh":[{"descriptor_ui":"D000069283","descriptor_name":"Rituximab","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D000970","descriptor_name":"Antineoplastic Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D002470","descriptor_name":"Cell Survival","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D009504","descriptor_name":"Neutrophils","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D010587","descriptor_name":"Phagocytosis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D015451","descriptor_name":"Leukemia, Lymphocytic, Chronic, B-Cell","qualifier_ui":"Q000188","qualifier_name":"drug therapy","is_major_topic":false},{"descriptor_ui":"D015451","descriptor_name":"Leukemia, Lymphocytic, Chronic, B-Cell","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":true},{"descriptor_ui":"D015451","descriptor_name":"Leukemia, Lymphocytic, Chronic, B-Cell","qualifier_ui":"Q000473","qualifier_name":"pathology","is_major_topic":false},{"descriptor_ui":"D018951","descriptor_name":"Antigens, CD20","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D061067","descriptor_name":"Antibodies, Monoclonal, Humanized","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1182/blood-2016-08-735605","doi":"https://doi.org/10.1182/blood-2016-08-735605","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood-2016-08-735605","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},{"id":"pmid:28288980","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/28288980","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"Journal Article"},{"id":"pmh:oai:boa.unimib.it:10281/446346","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/10281/446346","pdf_url":null,"source":{"id":"https://openalex.org/S4306401259","display_name":"BOA (University of Milano-Bicocca)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I66752286","host_organization_name":"University of Milano-Bicocca","host_organization_lineage":["https://openalex.org/I66752286"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.9476000070571899,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1553227584","https://openalex.org/W1559033760","https://openalex.org/W1600924978","https://openalex.org/W1619850380","https://openalex.org/W1815693934","https://openalex.org/W1890466892","https://openalex.org/W1897592311","https://openalex.org/W1935886040","https://openalex.org/W1969824500","https://openalex.org/W1995589036","https://openalex.org/W2009109361","https://openalex.org/W2010329820","https://openalex.org/W2012521762","https://openalex.org/W2017098129","https://openalex.org/W2017768282","https://openalex.org/W2021374814","https://openalex.org/W2021681226","https://openalex.org/W2027374210","https://openalex.org/W2029850226","https://openalex.org/W2042725832","https://openalex.org/W2056714255","https://openalex.org/W2088659831","https://openalex.org/W2091057822","https://openalex.org/W2106545303","https://openalex.org/W2115351802","https://openalex.org/W2120319857","https://openalex.org/W2122786380","https://openalex.org/W2132467087","https://openalex.org/W2135802989","https://openalex.org/W2141139539","https://openalex.org/W2143728536","https://openalex.org/W2146167520","https://openalex.org/W2146425185","https://openalex.org/W2146682305","https://openalex.org/W2152652891","https://openalex.org/W2312431486","https://openalex.org/W2397170433"],"related_works":["https://openalex.org/W2612572431","https://openalex.org/W2979718894","https://openalex.org/W4235428905","https://openalex.org/W2122786380","https://openalex.org/W2735723297","https://openalex.org/W2588549653","https://openalex.org/W2413351369","https://openalex.org/W161815630","https://openalex.org/W4406091381","https://openalex.org/W2049456898"],"abstract_inverted_index":{"Polymorphonuclear":[0],"neutrophils":[1],"(PMNs)":[2],"have":[3,24,35],"previously":[4],"been":[5],"reported":[6],"to":[7,38,45],"mediate":[8,32,178],"phagocytosis":[9,62,183],"of":[10,66,112,116,122,159,184,194],"anti-CD20-opsonized":[11,67,185],"B":[12,69,127,164,187],"cells":[13,128],"from":[14,125,162],"patients":[15,198],"with":[16,96,103,135,169,199,202],"chronic":[17],"lymphocytic":[18],"leukemia":[19],"(CLL).":[20],"However,":[21],"recent":[22],"data":[23],"suggested":[25,84],"that":[26,109,148,176],"PMNs,":[27],"like":[28],"macrophages,":[29],"can":[30],"also":[31],"trogocytosis.":[33,85],"We":[34,174],"performed":[36],"experiments":[37],"more":[39],"precisely":[40],"investigate":[41],"this":[42,195],"point":[43],"and":[44,49,81,101,114,131,189],"discriminate":[46],"between":[47,79],"trogocytosis":[48,149,180],"phagocytosis.":[50,153],"In":[51],"live-cell":[52],"time-lapse":[53],"microscopy":[54],"experiments,":[55],"we":[56,107,190],"could":[57,72],"not":[58,172],"detect":[59,73],"any":[60],"significant":[61,137],"by":[63,157],"purified":[64],"PMNs":[65,117,177],"CLL":[68,92,126,144,163,186,200],"cells,":[70,165,188],"but":[71,171],"only":[74],"the":[75,97,123,192],"repeated":[76],"close":[77],"contact":[78],"effectors":[80],"targets,":[82],"which":[83,166],"Similarly,":[86],"in":[87,139,197,206],"flow":[88],"cytometry":[89],"assays":[90],"using":[91],"B-cell":[93,145],"targets":[94],"labeled":[95],"membrane":[98,160],"dye":[99,124],"PKH67":[100],"opsonized":[102],"rituximab":[104,170,203],"or":[105,142,204],"obinutuzumab,":[106],"observed":[108],"a":[110,120],"mean":[111],"50%":[113],"75%":[115],"had":[118],"taken":[119],"fraction":[121],"at":[129],"3":[130],"20":[132],"hours,":[133],"respectively,":[134],"no":[136],"decrease":[138],"absolute":[140],"live":[141],"total":[143],"numbers,":[146],"confirming":[147],"occurs,":[150],"rather":[151,181],"than":[152,182],"Trogocytosis":[154],"was":[155,167],"accompanied":[156],"loss":[158],"CD20":[161],"evident":[168],"obinutuzumab.":[173],"conclude":[175],"mostly":[179],"discuss":[191],"implications":[193],"finding":[196],"treated":[201],"obinutuzumab":[205],"vivo.":[207]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":12},{"year":2022,"cited_by_count":12},{"year":2021,"cited_by_count":17},{"year":2020,"cited_by_count":13},{"year":2019,"cited_by_count":12},{"year":2018,"cited_by_count":16},{"year":2017,"cited_by_count":7}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2017-03-23T00:00:00"}